1. Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes
- Author
-
Konstantin G. Chernov, Vladislav V. Verkhusha, and Olena S. Oliinyk
- Subjects
0301 basic medicine ,animal structures ,allophycocyanin ,Infrared Rays ,cyanobacteriochrome ,Review ,Biosensing Techniques ,Biology ,Catalysis ,lcsh:Chemistry ,Inorganic Chemistry ,03 medical and health sciences ,Bacterial Proteins ,Light energy ,Physical and Theoretical Chemistry ,Absorbance spectra ,lcsh:QH301-705.5 ,Molecular Biology ,neoplasms ,Spectroscopy ,Fluorescent Dyes ,phytochrome ,bacterial photoreceptor ,030102 biochemistry & molecular biology ,Phytochrome ,Bacteria ,Organic Chemistry ,Near-infrared spectroscopy ,technology, industry, and agriculture ,Phycocyanin ,Far-red ,General Medicine ,equipment and supplies ,Fluorescence ,Computer Science Applications ,030104 developmental biology ,Synechocystis sp ,surgical procedures, operative ,lcsh:Biology (General) ,lcsh:QD1-999 ,Biochemistry ,near-infrared fluorescent protein ,tetrapyrrole ,Cyanobacteriochrome - Abstract
Bacterial photoreceptors absorb light energy and transform it into intracellular signals that regulate metabolism. Bacterial phytochrome photoreceptors (BphPs), some cyanobacteriochromes (CBCRs) and allophycocyanins (APCs) possess the near-infrared (NIR) absorbance spectra that make them promising molecular templates to design NIR fluorescent proteins (FPs) and biosensors for studies in mammalian cells and whole animals. Here, we review structures, photochemical properties and molecular functions of several families of bacterial photoreceptors. We next analyze molecular evolution approaches to develop NIR FPs and biosensors. We then discuss phenotypes of current BphP-based NIR FPs and compare them with FPs derived from CBCRs and APCs. Lastly, we overview imaging applications of NIR FPs in live cells and in vivo. Our review provides guidelines for selection of existing NIR FPs, as well as engineering approaches to develop NIR FPs from the novel natural templates such as CBCRs.
- Published
- 2017