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A porcine model was used to investigate the feasibility of using VIS-NIR spectroscopy to differentiate between degrees of ischemia–reperfusion injury in the small intestine. Ten pigs were used in this study and four segments were created in the small intestine of each pig: (1) control, (2) full arterial and venous mesenteric occlusion for 8 h, (3) arterial and venous mesenteric occlusion for 2 h followed by reperfusion for 6 h, and (4) arterial and venous mesenteric occlusion for 4 h followed by reperfusion for 4 h. Two models were built using partial least square discriminant analysis. The first model was able to differentiate between the control, ischemic, and reperfused intestinal segments with an average accuracy of 99.2% with 10-fold cross-validation, and the second model was able to discriminate between the viable versus non-viable intestinal segments with an average accuracy of 96.0% using 10-fold cross-validation. Moreover, histopathology was used to investigate the borderline between viable and non-viable intestinal segments. The VIS-NIR spectroscopy method together with a PLS-DA model showed promising results and appears to be well-suited as a potentially real-time intraoperative method for assessing intestinal ischemia–reperfusion injury, due to its easy-to-use and non-invasive nature. [ABSTRACT FROM AUTHOR]

Dry matter (DM) content is one of the most important quality features of potatoes. It defines the physical properties of the potatoes and determines what kind of product the potatoes can be used for. This paper presents the results obtained by a novel prototype NIR (near-infrared) instrument designed to measure DM content in single potatoes in process. The instrument is based on interaction measurements to measure deeper into the potatoes. It measures rapidly, up to 50 measurements per second, allowing several moving potatoes to be measured per second. The instrument also enables several interactance distances to be recorded for each measurement. The instrument was calibrated based on three different potato varieties and the calibration measurements were done in a process plant, making the calibration model suitable for in-line use. A good calibration for DM was obtained by partial least squares regression (RMSECV = 0.78% DM, R2 = 0.91). The instrument was tested in-line in the process plant and several batches of potatoes were monitored for the estimation of the DM distribution per batch. Accuracy of DM determination as function of measurement position on the potato was studied, and results indicate that NIR scans along the center part of the potatoes give slightly better results compared to scans taken on either side of the center. Small differences in optical measurement geometry influence the accuracy of the calibration models, underlining the importance of optimizing instrument design for successful measurements. [ABSTRACT FROM AUTHOR]