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Berberis vulgaris (L.) has remarkable ethnopharmacological properties and is widely used in traditional medicine. The present study investigated B. vulgaris stem bark (Berberidis cortex) by extraction with 50% ethanol. The main secondary metabolites were quantified, resulting in a polyphenols content of 17.6780 ± 3.9320 mg Eq tannic acid/100 g extract, phenolic acids amount of 3.3886 ± 0.3481 mg Eq chlorogenic acid/100 g extract and 78.95 µg/g berberine. The dried hydro-ethanolic extract (BVE) was thoroughly analyzed using Ultra-High-Performance Liquid Chromatography coupled with High-Resolution Mass Spectrometry (UHPLC–HRMS/MS) and HPLC, and 40 bioactive phenolic constituents were identified. Then, the antioxidant potential of BVE was evaluated using three methods. Our results could explain the protective effects of Berberidis cortex EC50FRAP = 0.1398 mg/mL, IC50ABTS = 0.0442 mg/mL, IC50DPPH = 0.2610 mg/mL compared to ascorbic acid (IC50 = 0.0165 mg/mL). Next, the acute toxicity and teratogenicity of BVE and berberine—berberine sulfate hydrate (BS)—investigated on Daphnia sp. revealed significant BS toxicity after 24 h, while BVE revealed considerable toxicity after 48 h and induced embryonic developmental delays. Finally, the anticancer effects of BVE and BS were evaluated in different tumor cell lines after 24 and 48 h of treatments. The MTS assay evidenced dose- and time-dependent antiproliferative activity, which was higher for BS than BVE. The strongest diminution of tumor cell viability was recorded in the breast (MDA-MB-231), colon (LoVo) cancer, and OSCC (PE/CA-PJ49) cell lines after 48 h of exposure (IC50 < 100 µg/mL). However, no cytotoxicity was reported in the normal epithelial cells (HUVEC) and hepatocellular carcinoma (HT-29) cell lines. Extensive data analysis supports our results, showing a significant correlation between the BVE concentration, phenolic compounds content, antioxidant activity, exposure time, and the viability rate of various normal cells and cancer cell lines. [ABSTRACT FROM AUTHOR]

Agroindustry waste has exponentially increased in recent years, generating economic losses and environmental problems. In addition, new ways to generate sustainable alternative electrical energy are currently being sought to satisfy energy demand. This investigation proposes using avocado waste as fuel for electricity generation in single-chamber MFCs. The avocado waste initially operated with an ambient temperature (22.4 ± 0.01 °C), DO of 2.54 ± 0.01 mg/L, TDS of 1358 ± 1 mg/L and COD of 1487.25 ± 0.01 mg/L. This research managed to generate its maximum voltage (0.861 ± 0.241 V) and current (3.781 ± 0.667 mA) on the fourteenth day, operating at an optimal pH of 7.386 ± 0.147, all with 126.032 ± 8.888 mS/cm of electrical conductivity in the substrate. An internal resistance of 67.683 ± 2.456 Ω was found on day 14 with a PD of 365.16 ± 9.88 mW/cm2 for a CD of 5.744 A/cm2. Micrographs show the formation of porous biofilms on both the anodic and cathodic electrodes. This study gives preliminary results of using avocado waste as fuel, which can provide outstanding solutions to agro-industrial companies dedicated to selling this fruit. [ABSTRACT FROM AUTHOR]

The present study evaluated the performance of some enzymatic and non-enzymatic antioxidant systems against oxidative stress for 10 to 30 d of refrigeration (R) and 15 to 50 d in controlled atmosphere (CA) conditions in both exocarp and mesocarp of Hass avocados from early and late harvests and at shelf life (SL) or consumption maturity. The possible relationship of the antioxidant systems with the occurrence of physiological disorders is also evaluated. The results indicate that the enzymatic system—superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), phenylalanine ammonium lyase (PAL) and polyphenoloxidase (PPO)—as well as the non-enzymatic system—such as phenolic compounds (PC)—showed different responses to the stress generated during storage and shelf life. In general, SOD, CAT, PAL and PPO did not significantly vary in storage (R or CA). At consumption maturity, SOD, POD and PAL activities decreased in the mesocarp (RSL and CASL), while CAT increased in the exocarp for CASL15-50d. PC instead decreased in the exocarp as the harvest period progressed while it increased in the mesocarp. Physiological disorders (dark spots) showed only in refrigeration on the exocarp at R30d and in mesocarp at RSL30d coincident with low SOD and low SOD and POD activity values, as well as low PC contents (p-coumaric and its derivatives and caffeic acid derivatives), respectively. The results support the use of CA as a postharvest technology to prevent the development of physiological disorders through the joint action of antioxidative defenses during avocado transport to distant markets until consumption maturity is reached. [ABSTRACT FROM AUTHOR]

Mulching suppresses weeds, improves soil biology, and increases physical or bioactive fruit yield in fruit orchards. However, there is no information on its impact on calafate (Berberis microphylla G. Forst.) orchards, which produce berries with high antioxidant content. To address this gap, in 2021, an experiment was conducted to evaluate the effect of 5 years of mulching on soil, plants, and calafate fruit. Four mulching treatments were established: no mulch (control), geotextile, oat straw, and hazelnut shell. All mulches suppressed weeds (43%) and maintained more soil moisture (5%) than the control. Soil microbial activity increased only with hazelnut shell compared with the control, up to 46%. Only oat straw and hazelnut shell increased basal respiration and urease up to 31% and 15% more than the control. Oat straw produced the highest fruit yield with 0.44 t ha−1, while the lowest yield was produced by the control and hazelnut shell with 0.1 and 0.15 t ha−1, respectively. The geotextile with 0.35 t ha−1 of fruit produced no differences between treatments. The ORAC antioxidant capacity was only higher in the control and hazelnut shell, with a mean of 3272 µmol TE 100 g−1. Hazelnut shell mulch is recommended to improve the biological functions of the soil and the antioxidant capacity of the calafate fruit. [ABSTRACT FROM AUTHOR]

To improve the yield and quality of wheat is of great importance for food security worldwide. One of the most effective and significant approaches to achieve this goal is to enhance the nitrogen use efficiency (NUE) in wheat. In this review, a comprehensive understanding of the factors involved in the process of the wheat nitrogen uptake, assimilation and remobilization of nitrogen in wheat were introduced. An appropriate definition of NUE is vital prior to its precise evaluation for the following gene identification and breeding process. Apart from grain yield (GY) and grain protein content (GPC), the commonly recognized major indicators of NUE, grain protein deviation (GPD) could also be considered as a potential trait for NUE evaluation. As a complex quantitative trait, NUE is affected by transporter proteins, kinases, transcription factors (TFs) and micro RNAs (miRNAs), which participate in the nitrogen uptake process, as well as key enzymes, circadian regulators, cross-talks between carbon metabolism, which are associated with nitrogen assimilation and remobilization. A series of quantitative genetic loci (QTLs) and linking markers were compiled in the hope to help discover more efficient and useful genetic resources for breeding program. For future NUE improvement, an exploration for other criteria during selection process that incorporates morphological, physiological and biochemical traits is needed. Applying new technologies from phenomics will allow high-throughput NUE phenotyping and accelerate the breeding process. A combination of multi-omics techniques and the previously verified QTLs and molecular markers will facilitate the NUE QTL-mapping and novel gene identification. [ABSTRACT FROM AUTHOR]

Genetic improvement for nitrogen use efficiency (NUE) can play a very crucial role in sustainable agriculture. Root traits have hardly been explored in major wheat breeding programs, more so in spring germplasm, largely because of the difficulty in their scoring. A total of 175 advanced/improved Indian spring wheat genotypes were screened for root traits and nitrogen uptake and nitrogen utilization at varying nitrogen levels in hydroponic conditions to dissect the complex NUE trait into its component traits and to study the extent of variability that exists for those traits in Indian germplasm. Analysis of genetic variance showed a considerable amount of genetic variability for nitrogen uptake efficiency (NUpE), nitrogen utilization efficiency (NUtE), and most of the root and shoot traits. Improved spring wheat breeding lines were found to have very large variability for maximum root length (MRL) and root dry weights (RDW) with strong genetic advance. In contrast to high nitrogen (HN), a low nitrogen (LN) environment was more effective in differentiating wheat genotypes for NUE and its component traits. Shoot dry weight (SDW), RDW, MRL, and NUpE were found to have a strong association with NUE. Further study revealed the role of root surface area (RSA) and total root length (TRL) in RDW formation as well as in nitrogen uptake and therefore can be targeted for selection to further the genetic gain for grain yield under high input or sustainable agriculture under limited inputs. [ABSTRACT FROM AUTHOR]

Purple corn seeds germinated at 25 °C and 35 °C for 5 days were used to obtain purple corn protein concentrate (PCPC25 and PCPC35). PCPC25 and PCPC35 were subject to gastrointestinal hydrolysis. PCPC and its hydrolysates were used to evaluate their functional and biological properties. Total flavonoid content (TFC), total flavonol content (TFLC), total anthocyanin content (TAC) and total proanthocyanin (TAP) were determined. The polyphenols and flavonoids were identified by UPLC-QDa. Protein solubility (PS), water and oil absorption capacity (WAC and OAC) were determined. The antioxidant activity was evaluated by the FRAP, ABTS and DPPH methods. PCPC35 showed the highest TFLC values (11,091.37 mg rutin equivalents (RE)/100 g dry weight DW), and TFL presented values of 7975.59 mg RE/100 g DW. The PCPCs presented better functional properties than the hydrolysates. PCPC25 presented a PS of 59.43%PS at pH 8.0, 27.77%WAC and 24.94%OAC. PCPC25 and PCPC35 showed high values of antioxidant activity. PCPC25 showed ABTS values (570.97 µmol trolox equivalents (TE)/g DW) and FRAP (772.85 µmol TE/g DW). PCPCs hydrolysates were less active with ABTS values (74.12 µmol TE/g DW) and FRAP (59.42 µmol TE/g DW). [ABSTRACT FROM AUTHOR]

To evaluate the potential health-promoting benefits of Berberis nummularia and B. atrocarpa fruits, the biochemical properties (nutrition component, mineral substance, organic acids), total phenolic and flavonoid content and antioxidant (DPPH, FRAP, ABTS and ORAC) capacity of ethanol extracts of B. nummularia and B. atrocarpa fruits wild-grown in Xinjiang were analyzed. The results indicated that there were no meaningful differences (p > 0.05) between the ash (1 ± 0.1 and 1 ± 0.0 g/100 g), fiber (16 ± 1.0 and 18 ± 1.4) and carbohydrate (57 ± 1.8 and 56 ± 1.8 g/100 g) content, respectively, in the dry fruits of B. nummularia and B. atrocarpa. The total fat (7 ± 0.4 and 5 ± 0.1 mg/100 g), soluble sugar (23 ± 0.6 and 12 ± 1.4 g/100 g), titratable acidity (18 ± 2.5% and 14 ± 1.3%) content, and energy value (330.86 and 314.41 kcal/100 g) of B. nummularia was significantly higher than that of B. atrocarpa fruits. Both species contain malic acid, acetic acid, tartaric acid, citric acid and fumaric acid, in which, malic acid is the dominant organic acid. The organic acid and mineral components of B. nummularia fruits were significantly higher than that of B. atrocarpa (p < 0.05). The total phenolic and flavonoid content of B. nummularia were 2 ± 0.0 mg GA/g DW and 2 ± 0.0 mg RE/g DW, respectively, which were significantly lower than the total phenolic and flavonoid content of B. atrocarpa (12 ± 0.1 mg GA/g DW and 9 ± 0.0 mg RE/g DW). The antioxidant capacity of B. nummularia (4 ± 0.1 mg Ascorbic acid/g DW for DPPH, 32 ± 0.1 mg Trolox/g DW for FRAP, 80 ± 3.0 mg Trolox/g DW for ABTS and 60 ± 3.6 mg Trolox/g for ORAC was significantly lower than that of B. atrocarpa (12 ± 0.0 mg Ascorbic acid/g DW for DPPH, 645 ± 1.1 mg Trolox/g DW for FRAP, 304 ± 3.0 mg Trolox/g DW for ABTS and 155 ± 2.8 mg Trolox/g for ORAC). B. atrocarpa fruits showed significantly higher antioxidant capacity than that of B. nummularia. The fruits of the two species can be used in food coloring and nutritional supplements, and consumption of the fruits can aid in weight control and reduce blood glucose or cholesterol. [ABSTRACT FROM AUTHOR]

Simple Summary: Experimental mice are the most commonly used laboratory animals for biomedical research and comparative studies. However, microbial infection may alter the mouse phenotype and confound interpretation results. The genus Corynebacterium, Gram-positive diphtheroid rod-shaped bacteria, induces severe diseases, such as hyperkeratosis and pseudotuberculosis, in immunodeficient mice. In this report, we described the population of Corynebacterium spp. isolated from laboratory mice in Korea using different approaches, comparing the accuracy and problems associated with each method. When identified based on molecular methods such as 16S rRNA and rpoB gene sequence analysis, the main Corynebacterium species were C. mastitidis (44.8%), C. bovis (25.5%), C. lowii (21.2%), and C. amycolatum (8.5%). In addition, matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) yielded results that were 77.9% identical to the molecular results, whereas biochemical methods showed only 15.5% identical to molecular identification. Collectively, our findings indicate that the different results may be obtained depending on the method used to identify Corynebacterium isolated from experimental mice, highlighting the importance of selecting an appropriate Corynebacterium identification method in obtaining accurate identification results. This result will help to increase the reliability of Corynebacterium diagnosis result from experimental mice. The genus Corynebacterium, composed of Gram-positive diphtheroid rod-shaped bacteria, induces severe diseases, such as Corynebacterium-associated hyperkeratosis and pseudotuberculosis, in immunodeficient mice. We isolated and identified a total of 165 strains of Corynebacterium species from experimental mice in Korean laboratories, diagnosed using several methods. When identified based on molecular methods, namely, 16S rRNA and rpoB gene sequence analysis, the main Corynebacterium species isolated in Korean laboratory mice were C. mastitidis (44.8%, n = 74), C. bovis (25.5%, n = 42), C. lowii (21.2%, n = 35), and C. amycolatum (8.5%, n = 14). Diagnoses were also performed using matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) and biochemical methods. MALDI-TOF MS yielded results that were 77.9% identical to the molecular identification results, whereas biochemical methods showed only 15.5% identical to molecular identification, partly owing to difficulties in distinguishing among C. mastitidis strains. Collectively, our findings indicate that molecular biological methods are better suited for detecting and identifying Corynebacterium species candidates isolated from mice than biochemical methods. Because of limitations associated with the use of MALDI-TOF MS, more precise results will be obtained by complementing this approach with other methods when used for rapid identification testing. [ABSTRACT FROM AUTHOR]

This study examined the leaves of Baccharis macrantha to obtain extracts of Baccharis macrantha (EBM) and to determine the total flavonoid content (TFC) and the total polyphenol content (TPC). The main objective of this work was to quantify TPC and TFC of extracts of B. macrantha from Ecuador and evaluate its antioxidant and anti-inflammatory activities and inhibition of lipid peroxidation. The extraction method was optimized with solvents, ethanol, and methanol, at temperatures of 30–60 °C and extraction times of 5–20 min. The optimal TFC extraction conditions were at EtOH25% at 50 °C for 10 min. The optimal TPC extraction conditions were at EtOH50% at 50 °C for 10 min. EBM was characterized by TLC and HPLC with three standards: gallic acid, catechin, and quercetin. EBM-EtOH25% and EBM-EtOH50% obtained at 50 °C for 10 min were used to identify quercetin and evaluate biologicals activities. Quercetin was detected in EBM (EtOH25% and EtOH50%). EBM anti-inflammatory activity was evaluated with the red blood cell stabilization (RBC) method. The RBC model showed values of 49.72% of protection lysis RBC to EBM-EtOH25% and 50.71% of protection lysis RBC to EBM-EtOH50%. The EBM in vitro inhibition of lipid peroxidation showed a protection of 77.00% (EtOH25%) and 73.11% (EtOH50%) when the TBARs method was used. EBM-EtOH25% and EtOH50% showed high antioxidant activity. EBM-EtOH25% presented values of ABTS (1172 µmol TE/g EBM), DPPH (836 µmol TE/g, EBM), and FRAP (85.70 µmol TE/g, EBM). [ABSTRACT FROM AUTHOR]

Simple Summary: The demand for consumption of protein of animal and vegetable origin increases with the growth of the world population. Therefore, new sources of animal protein that are nutritious, safe and sustainable for the environment are needed. In this situation, edible insects become a very attractive alternative due to their high protein content, their good functional and biological properties, and their environmental sustainability. Insects as food are not attractive to consumers who do not have them incorporated into their food culture, but products derived from them are accepted by consumers and can be used as functional ingredients by the food industry. Insect protein isolates and concentrates can be used as functional ingredients. The main objective of this work was to obtain protein concentrate from cricket (Gryllus assimilis) flour to evaluate their functional and biological properties. In addition to characterizing its protein profile and digestibility. Edible insects can represent an alternative to obtain high-quality proteins with positive biological properties for human consumption. Cricket flour (Gryllus assimilis) was used to obtain cricket protein concentrate (CPC) using pHs (10.0 and 12.0) of extraction and pHs (3.0, 4.0, 5.0, and 6.0) of isoelectric precipitation (pI). Protein content, water and oil absorption capacity, protein solubility, antioxidant, and anti-inflammatory activities were determined. In addition, the protein profile was characterized by electrophoresis and the in vitro CPC digestibility was evaluated. Cricket flour presented 45.75% of protein content and CPC 12–5.0 presented a value of 71.16% protein content using the Dumas method. All samples were more soluble at pH 9.0 and 12.0. CPC 12–3.0 presented a percentage of water-binding capacity (WBC) of 41.25%. CPC 12–6.0 presented a percentage of oil-binding capacity (OBC) of 72.93%. All samples presented a high antioxidant and anti-inflammatory activity. CPC 12–4.0 presented a value FRAP of 70,034 umol trolox equivalents (TE)/g CPC, CPC 12–6.0 presented a value ABTS of 124,300 umol TE/g CPC and CPC 10–3.0 presented a DPPH value of 68,009 umol TE/g CPC. CPC 10–6.0 and CPC 12–6.0 presented high anti-inflammatory activity, with values of 93.55% and 93.15% of protection, respectively. CPCs can be used as functional ingredients in the food industry for their excellent functional and biological properties. [ABSTRACT FROM AUTHOR]

Cereals are keystone crops for achieving food security and socioeconomic equilibrium, but rainfed cropland is highly sensitive to environmental anomalies that impact yields. The impact of soil moisture on cereal yield is particularly overlooked. This study evaluates the impact of root-zone soil moisture on yield compared to nine common climatic variables: maximum and minimum temperature, diurnal temperature range, growing degree days, accumulated rainfall, radiation, photothermal quotient, relative humidity of the air, and vapor pressure deficit. This study used the climatic database E-OBSv23 and the soil moisture databases ERA5-Land and LISFLOOD, focused on wheat and barley over the main cereal areas of Spain. Correlation analysis between annual yield and daily soil moisture and climatic data provided indicated the prevalence and concurrence of the impact factors on phenological stages of the Zadoks scale. Critical periods of impact on wheat and barley yields primarily concentrate during the growth and reproductive phases of spring. Soil moisture exceeds all other factors in magnitude and duration of influence, and our results suggest a complex interplay of factors during the critical spring period. This study highlights the preeminent role of soil moisture over climatic factors on the variability of rainfed cereal yields in water-limited areas. [ABSTRACT FROM AUTHOR]

Studies related to teacher training in primary education indicate a lack of knowledge, low levels of teaching self-efficacy, and negative emotions towards the teaching of physics. The main objective of this research was to compare the influence of two teaching methodologies on the learning and teaching self-efficacy of teachers-in-training on optics content related to light and color. The research design was quasi-experimental with control and experimental groups, a pre-test, and two post-tests. A non-probabilistic sample of 173 trainee primary school teachers was used. The control group used an academic–expositional teaching methodology, and the experimental group used a practical methodology based on the use of STEM teaching tools. Measuring instruments were designed and applied before and after the interventions to measure the evolution of cognitive and competence variables of the participating sample, depending on the methodology applied, both in the short and long term. The results reveal statistically significant improvements between the control group and the experimental group, both in the knowledge level variable and in the teaching self-efficacy variable. [ABSTRACT FROM AUTHOR]

The review describes the development of batch solid phase extraction procedures based on dispersive (micro)solid phase extraction with molecularly imprinted polymers (MIPs) and magnetic MIPs (MMIPs). Advantages and disadvantages of the various MIPs for dispersive solid phase extraction and dispersive (micro)solid phase extraction are discussed. In addition, an effort has also been made to condense the information regarding MMIPs since there are a great variety of supports (magnetite and magnetite composites with carbon nanotubes, graphene oxide, or organic metal framework) and magnetite surface functionalization mechanisms for enhancing MIP synthesis, including reversible addition-fragmentation chain-transfer (RAFT) polymerization. Finally, drawbacks and future prospects for improving molecularly imprinted (micro)solid phase extraction (MIMSPE) are also appraised. [ABSTRACT FROM AUTHOR]

Ionic liquids (ILs), known as environmentally benign "green" solvents, were developed as an optimal solvent for the green extraction and separation field. In this paper, an ionic liquid-based ultrasonic-assisted extraction (IL-UAE) of flavonoids (FVs) from bamboo leaves of Phyllostachys heterocycla was developed for the first time. First, 1-butyl-3-methylimidazolium bromide ([Bmim] Br), with the best extraction efficiency, was selected from fifteen ionic liquids with diverse structure, like carbon chains or anions. Then, the influencing parameters of ionic liquid (IL) concentration, liquid-solid ratio, ultrasonic time, and ultrasonic power, were investigated by single factor tests, and further optimized using response surface methodology (RSM). In the optimization experiment, the best conditions were 1.5 mol/L [BMIM]Br aqueous solution, liquid-solid ratio 41 mL/g, ultrasonic time 90 min, and ultrasonic power 300 W. Furthermore, the microstructures of bamboo leaves and the recovery of FVs and [BMIM]Br were also studied. Therefore, this simple, green and effective IL-UAE method has potentiality for the extraction of FVs from bamboo leaves for the large-scale operations. [ABSTRACT FROM AUTHOR]

Remote sensing offers a low-cost method for developing spatially continuous crop production statistics across large areas and through time. Nevertheless, it has been difficult to characterize the production of individual smallholder farms, given that the land-holding size in most areas of South Asia (<2 ha) is smaller than the spatial resolution of most freely available satellite imagery, like Landsat and MODIS. In addition, existing methods to map yield require field-level data to develop and parameterize predictive algorithms that translate satellite vegetation indices to yield, yet these data are costly or difficult to obtain in many smallholder systems. To overcome these challenges, this study explores two issues. First, we employ new high spatial (2 m) and temporal (bi-weekly) resolution micro-satellite SkySat data to map sowing dates and yields of smallholder wheat fields in Bihar, India in the 2014–2015 and 2015–2016 growing seasons. Second, we compare how well we predict sowing date and yield when using ground data, like crop cuts and self-reports, versus using crop models, which require no on-the-ground data, to develop and parameterize prediction models. Overall, sow dates were predicted well (R2 = 0.41 in 2014–2015 and R2 = 0.62 in 2015–2016), particularly when using models that were parameterized using self-report sow dates collected close to the time of planting and when using imagery that spanned the entire growing season. We were also able to map yields fairly well (R2 = 0.27 in 2014–2015 and R2 = 0.33 in 2015–2016), with crop cut parameterized models resulting in the highest accuracies. While less accurate, we were able to capture the large range in sow dates and yields across farms when using models parameterized with crop model data and these estimates were able to detect known relationships between management factors (e.g., sow date, fertilizer, and irrigation) and yield. While these results are specific to our study site in India, it is likely that the methods employed and the lessons learned are applicable to smallholder systems more generally across the globe. This is of particular interest given that similar high spatio-temporal resolution micro-satellite data will become increasingly available in the coming years. [ABSTRACT FROM AUTHOR]

A new ionic liquid loaded silica gel amine (SG-APTMS-N,N-EPANTf2) was developed, as an adsorptive material, for selective adsorption and determination of zirconium, Zr(IV), without the need for a chelating intermediate. Based on a selectivity study, the SG-APTMS-N,N-EPANTf2 phase showed a perfect selectivity towards Zr(IV) at pH 4 as compared to other metallic ions, including gold [Au(III)], copper [Cu(II)], cobalt [Co(II)], chromium [Cr(III)], lead [Pb(II)], selenium [Se(IV)] and mercury [Hg(II)] ions. The influence of pH, Zr(IV) concentration, contact time and interfering ions on SG-APTMS-N,N-EPANTf2 uptake for Zr(IV) was evaluated. The presence of incorporated donor atoms in newly synthesized SG-APTMS-N,N-EPANTf2 phase played a significant role in enhancing its uptake capacity of Zr(IV) by 78.64% in contrast to silica gel (activated). The equilibrium and kinetic information of Zr(IV) adsorption onto SG-APTMS-N,N-EPANTf2 were best expressed by Langmuir and pseudo second-order kinetic models, respectively. General co-existing cations did not interfere with the extraction and detection of Zr(IV). Finally, the analytical efficiency of the newly developed method was also confirmed by implementing it for the determination of Zr(IV) in several water samples. [ABSTRACT FROM AUTHOR]

A series of imidazolium and pyridinium ionic liquids with different anions (Cl–, Br–, BF4–, PF6–) has been evaluated for their adsorption activity on silica gel. Quantification of the ionic liquids has been performed by the use of RP-HPLC with organic-aqueous eluents containing an acidic buffer and a chaotropic salt. Pseudo-second order kinetic models were applied to the experimental data in order to investigate the kinetics of the adsorption process. The experimental data showed good fitting with this model, confirmed by considerably high correlation coefficients. The adsorption kinetic parameters were determined and analyzed. The relative error between the calculated and experimental amount of ionic liquid adsorbed at equilibrium was within 7%. The effect of various factors such as initial ionic liquid concentration, temperature, kind of solvent, kind of ionic liquid anion and cation on adsorption efficiency were all examined in a lab-scale study. Consequently, silica gel showed better adsorptive characteristics for imidazolium-based ionic liquids with chaotropic anions from aqueous solutions in comparison to pyridinium ionic liquids. The adsorption was found to decrease with the addition of organic solvents (methanol, acetonitrile) but it was not sensitive to the change of temperature in the range of 5–40 °C. [ABSTRACT FROM AUTHOR]

Morocco belongs to the countries ranked at a high-risk level for entry, establishment, and spread of Xylella fastidiosa, which has recently re-emerged as a plant pathogen of global importance causing olive quick decline syndrome (OQDS). Symptomatic infection by X. fastidiosa leads to devastating diseases and important economic losses. To prevent such losses and damages, countries without current outbreaks like Morocco need to first understand their host plant responses to X. fastidiosa. The assessment of the macro and micro-elements content (ionome) in leaves can give basic and useful information along with being a powerful tool for the sustainable management of diseases caused by this devastating pathogen. Herein, we compare the leaf ionome of four important autochthonous Moroccan olive cultivars ('Picholine Marocaine', 'Haouzia', 'Menara', and 'Meslalla'), and eight Mediterranean varieties introduced in Morocco ('Arbequina', 'Arbosana', 'Leccino', 'Ogliarola salentina', 'Cellina di Nardo', 'Frantoio', 'Leucocarpa', and 'Picholine de Languedoc'), to develop hypotheses related to the resistance or susceptibility of the Moroccan olive trees to X. fastidiosa infection. Leaf ionomes, mainly Ca, Cu, Fe, Mg, Mn, Na, Zn, and P, were determined using inductively coupled plasma optical emission spectroscopy (ICP-OES). These varieties were also screened for their total phenolics and flavonoids content. Data were then involved in a comparative scheme to determine the plasticity of the pathogen. Our results showed that the varieties 'Leccino', 'Arbosana', 'Arbequina' consistently contained higher Mn, Cu, and Zn and lower Ca and Na levels compared with the higher pathogen-sensitive 'Ogliarola salentina' and 'Cellina di Nardò'. Our findings suggest that 'Arbozana', 'Arbiquina', 'Menara', and 'Haouzia' may tolerate the infection by X. fastidiosa to varying degrees, provides additional support for 'Leccino' having resistance to X. fastidiosa, and suggests that both 'Ogliarola salentina' and 'Cellina di Nardö' are likely sensitive to X. fastidiosa infection. [ABSTRACT FROM AUTHOR]

The authenticity and typicity of monocultivar oils and knowledge of the changes that environmental olive growing conditions bring to naturally present antioxidants and sensory attributes of virgin olive oils (VOO) are important for quality and safety improvement. This study delivers a comprehensive evaluation of the factors affecting phenolics, fatty acid composition and sensory characteristics of cultivars Oblica and Leccino VOOs throughout ripening season at two distinct olive growing environments during three consecutive crop years, and ranks the importance of each factor. Specified parameters were significantly influenced by olive growing environmental conditions. At the colder location of higher altitude, both cultivars gained higher amount of stearic, linoleic and linolenic fatty acids, as well as a higher proportion of phenolic compounds, but lower amounts of oleic fatty acid. At the warmer location of lower altitude, both cultivars had oils with lower level of fruitiness, bitterness and pungency. Analysis of the main components showed that VOOs were primarily differentiated by the cultivar, then main groups were divided with regard to the growing site, while harvest period affected the biosynthesis of natural VOOs antioxidants but had the least impact. These results reveal that the composition of fatty acids, phenolic content and sensory profile are predominantly characteristics of a cultivar. [ABSTRACT FROM AUTHOR]

We describe a new on-line sensitive and selective procedure for the determination of trivalent and hexavalent chromium in liquid samples by a tailor-made contactor (TMC), specifically a liquid membrane annular TMC, coupled with inductively coupled plasma with optical detection. The TMC was designed and developed to integrate the extraction and stripping phases of the analyte in one module to minimize the membrane solvent's consumption and maximize the speed of transport through the liquid membrane. Moreover, the particular geometry studied, which consists of two coaxial hollow fibers, allows the TMC to be used for both separating and preconcentrating purposes. Both (−)-N-dodecyl-N-methylephedrinium bromide (30 mM) in dichloroethane and HNO3 (0.75 M) were used as the liquid membrane and receiving solution, respectively. The proposed method's performance was evaluated in terms of the hexavalent chromium extraction efficiency and the coefficient of variation percentages; these were higher than 85% and less than 5%, respectively. In addition, the proposed procedure was applied to two real samples: a tap water sample and an eluate from solid urban waste. In both cases, the analytical performances were good and comparable to those obtained using synthetic standard solutions. [ABSTRACT FROM AUTHOR]

This work studied the effects of centrifugal block freeze crystallization (CBFC) on physicochemical parameters, total phenolic compound content (TPCC), antioxidant activity (AA), and process parameters applied to fresh murta and arrayan juices. In the last cycle, for fresh murta and arrayan juices, the total soluble solids (TSS) showed values close to 48 and 54 Brix, and TPCC exhibited values of approximately 20 and 66 mg gallic acid equivalents/100 grams dry matter (d.m.) for total polyphenol content, 13 and 25 mg cyanidin-3-glucoside equivalents/100 grams d.m. for total anthocyanin content, and 9 and 17 mg quercetin equivalents/100 grams d.m. for total flavonoid content, respectively. Moreover, the TPCC retention indicated values over 78% for murta juice, and 82% for arrayan juice. Similarly, the AA presented an increase over 2.1 times in relation to the correspondent initial AA value. Thus, the process parameters values were between 69% and 85% for efficiency, 70% and 88% for percentage of concentrate, and 0.72% and 0.88 (kg solutes/kg initial solutes) for solute yield. Therefore, this work provides insight about CBFC on valuable properties in fresh Patagonian berries juices, for future applications in health and industrial scale. [ABSTRACT FROM AUTHOR]

The phenolic composition and mineral content of Cornezuelo, Cornicabra and Picual olive fruit varieties were investigated during olive ripening in two different harvesting seasons (2017/2018 and 2018/2019). Phytochemical profiles were evaluated by high-performance liquid chromatography (HPLC) with diode-array and mass spectrometry detection. Mineral contents were determined by inductively coupled plasma-mass spectrometry (ICP-MS). Twenty-five compounds were characterized and the main ones quantified. These compounds corresponded mostly to secoiridoids, the main ones being oleuropein, oleoside/secologanoside, oleoside-11-methylester, and oleuropein and comselogoside isomers. Total phenolic contents reached the highest values between December and January, coinciding with the usual harvesting date. This trend was observed in both harvesting seasons, although higher phenolic contents were recorded in season 2018/2019. This was due to the different weather conditions, which caused a lower olive production in season 2017/2018. No clear tendency was observed between mineral content and harvest time in any of the studied seasons. The highest concentration of total phenolics was obtained in Cornezuelo variety (840 mg/100 g) in January 2019 (season 2018/2019). Picual and Cornicabra varieties reached concentrations of 670 mg/100 g and 530 mg/100 g, respectively, also in the last harvesting dates of season 2018/2019. [ABSTRACT FROM AUTHOR]

Corynebacterium species are commonly found in the conjunctiva of healthy adults and are recognized as non-pathogenic bacteria. In recent years, however, Corynebacterium species have been reported to be potentially pathogenic in various tissues. We investigated Corynebacterium species on the ocular surface and reviewed various species of Corynebacterium in terms of their antimicrobial susceptibility and the underlying molecular resistance mechanisms. We identified a risk for Corynebacterium-related ocular infections in patients with poor immunity, such as patients with diabetes or long-term users of topical steroids, and in those with corneal epithelial damage due to trauma, contact lens wear, lagophthalmos, and trichiasis. The predominant strain in the conjunctiva was C. macginleyi, and the species associated with keratitis and conjunctivitis were C. macginleyi, C. propinquum, C. mastitidis, C. pseudodiphtheriticum, C. accolens, C. striatum, C. xerosis, and C. bovis. Overall, Corynebacterium species present on the ocular surface were resistant to quinolones, whereas those in the nasal cavity were more susceptible. The prevalence of fluoroquinolone-resistant Corynebacterium has not changed in the past 10 years; however, Corynebacterium species remain susceptible to third-generation cephems. In conclusion, the use of third-generation cephems should be a reasonable and pragmatic approach for treatment of ocular infections caused by Corynebacterium species. [ABSTRACT FROM AUTHOR]

Wheat is a major cool-season forage crop in the southern United States. The objective of this study is to understand the effect of nitrogen (N) fertilization on wheat biomass yield, quality, nitrogen use efficiency (NUE), and nitrogen nutrition index (NNI). The experiments were conducted in a greenhouse and a hoop house in a split-plot design, with three replications. Twenty wheat cultivars/lines were evaluated at four N rates (0, 75, 150, and 300 mg N.kg−1 soil) in the greenhouse and (0, 50, 100, and 200 mg N.kg−1 soil) in the hoop house. In general, high-NUE lines had lower crude protein content than the low-NUE lines. None of the cultivars/lines reached a plateau for biomass production or crude protein at the highest N rate. The line × N rate interaction for NUE was not significant in the greenhouse (p = 0.854) but was highly significant in the hoop house (p < 0.001). NNI had a negative correlation with NUE and biomass. NUE had strong positive correlations with shoot biomass and total biomass but low to moderate correlations with root biomass. NUE also had a strong positive correlation with N uptake efficiency. Lines with high NUE can be used in breeding programs to enhance NUE in wheat for forage use. [ABSTRACT FROM AUTHOR]

Ecuador Andean purple corn (Zea mays L.) was subjected to a germination process at 15–40 °C for 24–168 incubation hours. Purple corn protein concentrates (PCPCs) were obtained by alkaline extraction at pH 8.0 and pH 10.0, followed by an isoelectric precipitation process at pH 4.0, pH 5.0 and pH 6.0. Proteins and phenolic content of PCPCs was calculated. PCPC antioxidant properties were determined by the ferric-reducing antioxidant power (FRAP) in vitro method and by the 2,2-azinobis, 3-ethyl-benzothiazoline-6-sulfonic acid, (ABTS) in vitro method. Andean purple corn seeds were able to germinate under the germination conditions tested in this study. The higher percentage of germination was of 63.33% at 168 h/25 °C. The PCPCs protein profile was characterized for the presence of six bands with molecular weights of 14.50 kDa, 20.12 kDa, 25.18 kDa, 41.85 kDa, 59.59 kDa, and 65.87 kDa. Germinated PCPC presented a high TPC content with ranges of 605.71–1820.00 mg gallic acid equivalents (GAE)/g PCPC dry weight (DW), germinated PCPC/72 h/25 °C presented a higher value of 1820.00 mg GAE/g PCPC, DW. All germinated PCPCs samples assayed presented strong antioxidant activity when measured by the ABTS and FRAP methods. Germinated PCPC/144 h/35 °C presented high antioxidant activity by ABTS with 804.35 µmol Trolox equivalents (TE)/g PCPC DW and germinated PCPC/144 h/30 °C presented a high value by the FRAP method, 987.83 µmol TE/g PCPC DW. [ABSTRACT FROM AUTHOR]

Andean blackberries (Rubus glaucus Benth) are fruits rich in phytocomponents with high antioxidant activity. In this work, the changes in the total polyphenol content (TPC), the total flavonoid content (TFC), and the total anthocyanin content (TAC) of four blackberry varieties at three maturity stages (E1-25%, E2-50%, and E3-100%) were measured. The antioxidant activity (AA) was evaluated using the 2,2'azinobis-(3-ethylbenzthiazolin 6-sulphonic acid (ABTS) and ferric reducing antioxidant power (FRAP) methods. TPC and TFC content decreased with the increase in the maturity stage. The blackberry Brazos cultivar presented TPC values of 51.26, 38.16, and 31.59 mg of gallic acid equivalents (GAE)/g dry weight (DW) at E1, E2, and E3, respectively. The TAC and soluble solids increased with the increase in the maturity stage of the fruits. The Andimora variety at E3 presented a high TPC content, and the Colombiana variety presented a high TFC content. The blackberry Colombiana cultivar presented TAC values of 1.40, 2.95, and 12.26 mg cy-3-glu/100g DW at E1, E2, and E3, respectively. The blackberry Colombiana cultivar presented a high AA value at 1278.63 µmol TE/g DW according to the ABTS method and 1284.55 µmol TE/g DW according to the FRAP method. The TPC and TFC showed a high correlation with the AA according to the ABTS and the FRAP methods. The Pearson correlation between the TFC and AA/ABTS has a value of r = 0.92. The TFC and AA/FRAP present a value of r = 0.94. [ABSTRACT FROM AUTHOR]

Global nitrogen use efficiency (NUE) for cereal production is marginal and is estimated to be about 33%. Remote sensing tools have tremendous potential for improving NUE in crops through efficient nitrogen management as well as the identification of high-NUE genotypes. The objectives of this study were (i) to identify and quantify the variation in NUE across 24 winter wheat genotypes (Triticum aestivum L.) and (ii) to determine if the normalized difference vegetation index (NDVI) could characterize the variability in NUE across wheat genotypes. This study was conducted in 2010 and 2011 in the semi-arid climate of Northeastern Colorado across dryland and irrigated conditions. Our results indicate significant variation in the NUE among genotypes across two irrigation conditions. We observed a strong relationship between the NDVI and NUE—as PFP (partial factor productivity) and PNB (partial nitrogen balance)—across the 24 wheat genotypes under dryland conditions (average R2 for PFP and PNB = 0.84) at Feekes growth stage 11.1, for site year II. However, poor association was observed under irrigated conditions (average R2 for PFP and PNB = 0.29) at Feekes growth stage 3 to 4 for site year II. This study demonstrates the potential and limitations of active canopy sensing to successfully characterize the variability in NUE across wheat genotypes. [ABSTRACT FROM AUTHOR]

Grain number and weight within a spikelet are major yield components which determine the grain yield in wheat. The objective of this study was to explore genetic gains in grain performance within wheat spikelets at the individual grain level and its effect on grain yield and evaluate genetic progress in stem internode length and other yield-related traits. We conducted field experiments across three growing seasons in the western Yellow and Huai Valley of China; incorporating 17 bread wheat cultivars released from 1948 to 2012. Yields were significantly correlated with year of release. Yield gains equated to 3.95 g m−2 yr−1 in response to increases in total grain weight per m2 (GW) in proximal (G1 and G2) and distal (G3 and G4) grains, despite a decline in the proportional contribution of proximal grains to yield and increase in the proportional contribution of distal grains to yield with year of release. Grain number per m2 (GN), thousand-grain weight (TGW), and harvest index (HI) increased with year of release, but plant height decreased. Both grain number per spikelet of proximal and distal grain contributed to the increase in total GN. However, the contribution ratio of GN in proximal grains to total GN declined, and the proportion in distal grains increased. Average single grain weight (SGW) increased linearly at G1, G2, G3, and G4 with year of release and contributed to the increase in TGW. The G3 and G4 grain positions had much lower individual grain weights but increased at a faster rate than G1 and G2. At G1, G2, and G3 grain positions, from bottom to top spikelets, the newly released cultivars had the heaviest grains and the old cultivars had the lightest grains. New cultivars had more spikelets than old cultivars and the number of grains (proximal and distal grains) in the apical spike increased with year of release. The length of five internodes decreased significantly with year of release, more so in the upper than lower internodes, all of which contributed to the decline in plant height. In summary, increasing the number and weight of distal grains could increase grain yield, TGW, and GN. [ABSTRACT FROM AUTHOR]

In India, a significant reduction of wheat yield would cause a widespread impact on food security for 1.35 billion people. The two highest wheat producing states, Punjab and Haryana in northern India, experienced a prolonged period of anomalously low wheat yield during 2002–2010. The extent of climate variability and change in influencing this prolonged reduction in wheat yield was examined. Daily air temperature (Tmax and Tave) was used to calculate the number of days above optimum temperature and growing degree days (GDD) anomaly. Two drought indices, the standard precipitation and evapotranspiration index and the radiation-based precipitation index, were used to describe the drought conditions. Groundwater variability was assessed via satellite-based approximation. The analysis results indicate that the wheat yield loss corresponds to the increase in the number of days with a temperature above 35 °C during the maturity stage (March). Reduction in monsoon rainfall led to a depletion of groundwater and reduced surface water for irrigation in the wheat growing season (November–March). Higher temperatures, coupled with water shortage and irregular irrigation, also appear to impact the yield reduction. In hindsight, improving the agronomic practices to minimize crop water usage could be an adaptation strategy to maintain the desired wheat yield in the face of climate-induced drought and precipitation anomaly. [ABSTRACT FROM AUTHOR]