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The purpose of this study is to characterize the ocular surface microbiota of patients with an anophthalmic cavity. An eNAT with 1 mL of Liquid Amies Medium was used to collect samples. Microbial DNA from anophthalmic socket and healthy fellow control eye samples was isolated and sequenced. Raw reads were analyzed with GAIA (v 2.02). The richness and Shannon alpha diversity metrics, as well as Bray–Curtis beta diversity and Wilcoxon signed-rank test values, were computed with R packages such as phyloseq, mia, or DESeq2 to allow for microbiome analysis. Principal coordinate analysis (PCoA) was performed using the function plotReducedDim from the R package scater. The different taxonomic profiles were described under the concept of eye community state type (ECST). The microbiomes of both eyes from 25 patients with an anophthalmic cavity were analyzed in this study. While the microbial communities of paired eyes from the same patients showed notable dissimilarity, no consistent patterns emerged when comparing healthy eyes to anophthalmic sockets. Alpha diversity values did not significantly differ between healthy eyes and anophthalmic socket samples, though there was considerable variability within each group. Notably, anophthalmic socket samples generally exhibited lower abundances of genera such as Staphylococcus, Enterococcus, Paenibacillus, and Sediminibacterium compared to their healthy counterparts. Microbial variability between healthy eyes and anophthalmic sockets may be due to anatomical differences. Further research is needed to determine whether patients without anophthalmic sockets exhibit similar microbiome patterns in both eyes. [ABSTRACT FROM AUTHOR]

Extensive livestock farming plays a crucial role in the economy of agrosilvopastoral systems of the southwestern Iberian Peninsula (known as dehesas and montados in Spanish and Portuguese, respectively) as well as providing essential ecosystem services. The existence of livestock in these areas heavily relies on the effective management of natural resources (annual pastures and water stored in ponds built ad hoc). The present work aims to assess the water availability in these ponds by developing equations to estimate the water volume based on the surface area, which can be quantified by means of remote sensing techniques. For this purpose, field surveys were carried out in September 2021, 2022 and 2023 at ponds located in representative farms, using unmanned aerial vehicles (UAVs) equipped with RGB sensors and survey-grade global navigation satellite systems and inertial measurement units (GNSS-IMU). These datasets were used to produce high-resolution 3D models by means of Structure-from-Motion and Multi-View Stereo photogrammetry, facilitating the estimation of the stored water volume within a Geographic Information System (GIS). The Volume–Area–Height relationships were calibrated to allow conversions between these parameters. Regression analyses were performed using the maximum volume and area data to derive mathematical models (power and quadratic functions) that resulted in significant statistical relationships (r2 > 0.90, p < 0.0001). The root mean square error (RMSE) varied from 1.59 to 17.06 m3 and 0.16 to 3.93 m3 for the power and quadratic function, respectively. Both obtained equations (i.e., power and quadratic general functions) were applied to the estimated water storage in similar water bodies using available aerial or satellite imagery for the period from 1984 to 2021. [ABSTRACT FROM AUTHOR]

When analyzing occupational safety factors, a human factor is associated with 80–90% of incidents and accidents that occur. Controlling this factor is essential when it comes to creating healthy and safe organizations. Personality traits have shown great relevance when understanding the behavior of safety or self-protection in the workplace, affecting the motivation of subjects and, therefore, their behavior. The objective of this study is to analyze the influence of the extraversion trait on compliance with safety rules and behaviors, while evaluating the mediating effect of motivation for safety in health professionals. The sample is composed of 183 nurses and wardens of a hospital in Spain. The obtained results confirm the hypotheses raised, validating the negative influences of extraversion on motivation for security and compliance with norms, behaviors, and security, while verifying the effect of the total mediation of motivation for security in the influence of extraversion on security compliance. This confirms the effect of this personality trait on employees' safety behaviors, and the possibility of reducing this influence by controlling personal motivations with interventions from the organization. [ABSTRACT FROM AUTHOR]

Cigarette butts (CBs) are non-biodegradable harmful residues of synthetic origin and are widespread in marine environments around the world. Although environmental factors are often primarily responsible for the fragmentation of microplastics in the marine environment, biotic factors have recently been shown to be equally important in plastic debris. This study evaluates the role of the Hediste diversicolor polychaete in the fragmentation of CBs in the marine environment. Polychaetes were exposed to three concentrations of CB (0 (as the control), 0.25, and 1 butt L−1) at two different temperatures (15 °C and 23 °C) for 28 days. At each temperature, aquaria without polychaetes were used to study the effect of the burrowing activity of the polychaete on CB fragmentation. Toxicants analysed from exposed sediments increased their concentration in a dose-dependent manner to the CB concentration at a temperature of 15 °C but not at 23 °C. CBs did not directly decrease Hediste survival, but prolonged elevated temperatures increased the polychaetes' susceptibility. The negative effects of CBs on burial success and burrowing behaviour could not be offset by the reduced start time caused by elevated temperatures. Regardless of temperature, both the weight loss and physical fragmentation of CBs buried in polychaete-contaminated sediments were significantly higher than those without Hediste, with no differences between the two concentrations tested. FTIR-ATR analysis used to evaluate CB degradation in relation to cellulose acetate decomposition showed a greater degradation of this compound in treatments with Hediste than in those without polychaetes (~2.75 times), but these differences were not significant. This study is a promising initial step for future research, as any factor that facilitates the fragmentation of this prevalent and hazardous waste must be carefully studied to extract the maximum benefit to help to reduce CBs in the marine environment. [ABSTRACT FROM AUTHOR]

In the present paper, we address the following general question in the framework of classical first-order logic. Assume that a certain mathematical principle can be formalized in a first-order language by a set E of conditional formulas of the form α (v) → β (v) . Given a base theory T, we can use the set of conditional formulas E to extend the base theory in two natural ways. Either we add to T each formula in E as a new axiom (thus obtaining a theory denoted by T + E ) or we extend T by using the formulas in E as instances of an inference rule (thus obtaining a theory denoted by T + E – Rule ). The theory T + E will be stronger than T + E – Rule , but how much stronger can T + E be? More specifically, is T + E conservative over T + E – Rule for theorems of some fixed syntactical complexity Γ ? Under very general assumptions on the set of conditional formulas E, we obtain two main conservation results in this regard. Firstly, if the formulas in E have low syntactical complexity with respect to some prescribed class of formulas Π and in the applications of E – Rule side formulas from the class Π and can be eliminated (in a certain precise sense), then T + E is ∀ B (Π) -conservative over T + E – Rule . Secondly, if, in addition, E is a finite set with m conditional sentences, then nested applications of E – Rule of a depth at most of m suffice to obtain ∀ B (Π) conservativity. These conservation results between axioms and inference rules extend well-known conservation theorems for fragments of first-order arithmetics to a general, purely logical framework. [ABSTRACT FROM AUTHOR]

Purpose: The purpose of this study was to check the efficacy and safety of a novel tear substitute containing hyaluronic acid and low-dose hydrocortisone in the treatment of moderate dry eye disease. Methods: In this prospective randomized study, 38 patients with moderate dry eye disease were divided into two treatment groups: Group 1 received one drop of 0.2% sodium hyaluronate and 0.001% hydrocortisone four times daily for 3 months, while Group 2 received 0.15% sodium hyaluronate and 3% trehalose at the same dosage. OSDI and SANDE questionnaires, Non-Invasive Break-Up time (NIBUT), Tear Meniscus Height (TMH), meibography, Lipid Layer Thickness (LLT), Tear Break-Up Time (TBUT), Corneal Staining Score (CFS), and Intraocular Pressure (IOP) were evaluated at baseline and after 1, 2, and 3 months of treatment. Results: During the treatment period, Group 1 showed statistically significant improvement in OSDI score (p = 0.002), SANDE score (p = 0.01), NIBUT (p < 0.0001), LLT (p < 0.0001), TBUT (p = 0.01), and CFS (p = 0.02). In Group 2, significant improvement was observed only in the TBUT score (p < 0.05). Comparison of the two groups showed that NIBUT and LLT were significantly different at the end of treatment (p = 0.001 for both comparisons), with more favorable results for sodium hyaluronate and hydrocortisone than for sodium hyaluronate and trehalose. No significant variations in intraocular pressure were observed in either group during the treatment period (p > 0.05). Conclusions: The study confirms that a 3-months treatment with hyaluronic acid 0.2% in combination with low-dose hydrocortisone 0.001% improves the signs and symptoms of moderate DED and that a low-dosage 0.001% hydrocortisone can be helpful in preventing the progression to chronic stages of DED. [ABSTRACT FROM AUTHOR]

Transparent ZnMn2O4 thin films on indium tin oxide (ITO) were prepared through spray pyrolysis and implemented as electrodes in symmetric supercapacitors (SSCs). A specific capacitance value of 752 F g−1 at 0.5 A g−1 and a 70% retention over 3000 galvanostatic charge–discharge (GCD) cycles were reached with a 1.0 M Na2SO4 electrolyte in a three-electrode electrochemical cell. Analysis of the cycled electrodes with 1.0 M Na2SO4 revealed a local loss of electrode material; this loss increases when electrodes are used in SCCs. To avoid this drawback, solid polyvinylpyrrolidone-LiClO4 (PVP-LiClO4) and quasi-solid polyvinylpyrrolidone-ionic liquid (PVP-ionic liquid) electrolytes were tested in SSCs as substitutes for aqueous Na2SO4. An improvement in capacitance retention without a loss of electrode material was observed for the PVP-ionic liquid and PVP-LiClO4 electrolytes. With these non-aqueous electrolytes, the tetragonal structure of the ZnMn2O4 spinel was maintained throughout the cyclic voltammetry (CV) cycles, although changes occurred in the stoichiometry from ZnMn2O4 to Mn-rich Zn1−xMn3−xO4. In the case of the electrolyte 1.0 M Na2SO4, the loss of Zn2+ led to the formation of MnO2 via Zn1-xM3-xO4. The location of the three SCCs in the Ragone plot shows supercapacitor behavior. The electrochemical results prove that the pseudocapacitance is the major contributor to the electrode capacitance, and the SCCs can therefore be considered as pseudocapacitors. [ABSTRACT FROM AUTHOR]

The inherent capacity of all organisms to evolve under selective pressure has created multidrug-resistant microbes and cells that are now threatening decades of progress in human health introduced by the advent of antibiotics. This resistance is developed by all pathogens, from bacteria to cancer cells, through fungi, parasites, or the seemingly simpler entities, i.e., viruses. In this review, we give an overview on this phenomenon, describing the mechanisms by which resistant organisms manage to evade the action of drugs. We also review existing therapies, including some of the most recent. This bibliographic review shows how, despite the encouraging progress that has been achieved in many areas, a permanent effort from scientists is necessary and will always be needed in order to compensate for the continuous microbial response intrinsically linked to the evolutionary process. [ABSTRACT FROM AUTHOR]

Heart disease is a major contributor to mortality and disability on a global scale. Hence, there is a need for research to improve non-invasive diagnostic techniques. Diseases in dogs with characteristics very similar to those of human pathologies hold promise as a source of data for evaluating and developing echocardiographic techniques and devices. Methods: We conducted a structured literature search from June 2022 to January 2023 to evaluate the relevance of dogs as a translational model for echocardiographic clinical research. We searched various academic databases, including PubMed Central (PMC), Core, DIGITAL.CSIC, DOAB, DOAJ, EBSCO host, Elsevier B.V, Redib, Scopus, and Web of Science, available through the Academic Information System of the Autonomous University of Baja California. Results: Out of the 243 articles initially screened, we identified 119 relevant articles that met our inclusion criteria for further analysis. This review is an introduction to the canine model by analyzing the cardiovascular anatomical similarities between the two species, the pathophysiological overlaps in some diseases, the parallels in echocardiographic techniques in dogs compared to humans, and the suitability of dogs with a naturally occurring cardiac disease as a model for translational clinical research compared to other animal species. Conclusions: This review emphasizes the importance of canine patients as an ideal cardiac disease symmetrical clinical model since they share common heart diseases with humans. Furthermore, dogs have a shorter lifespan, leading to the relatively rapid evolution of these diseases, which makes studying these pathologies and developing echocardiographic techniques more feasible. The results strongly indicate the need for interdisciplinary collaboration and translational medical research to create innovative echocardiographic technologies and improve the connection between veterinary and human cardiac imaging research. [ABSTRACT FROM AUTHOR]

Background: SAAP-148 is an antimicrobial peptide derived from LL-37. It exhibits excellent activity against drug-resistant bacteria and biofilms while resisting degradation in physiological conditions. Despite its optimal pharmacological properties, its mechanism of action at the molecular level has not been explored. Methods: The structural properties of SAAP-148 and its interaction with phospholipid membranes mimicking mammalian and bacterial cells were studied using liquid and solid-state NMR spectroscopy as well as molecular dynamics simulations. Results: SAAP-148 is partially structured in solution and stabilizes its helical conformation when interacting with DPC micelles. The orientation of the helix within the micelles was defined by paramagnetic relaxation enhancements and found similar to that obtained using solid-state NMR, where the tilt and pitch angles were determined based on 15N chemical shift in oriented models of bacterial membranes (POPE/POPG). Molecular dynamic simulations revealed that SAAP-148 approaches the bacterial membrane by forming salt bridges between lysine and arginine residues and lipid phosphate groups while interacting minimally with mammalian models containing POPC and cholesterol. Conclusions: SAAP-148 stabilizes its helical fold onto bacterial-like membranes, placing its helix axis almost perpendicular to the surface normal, thus probably acting by a carpet-like mechanism on the bacterial membrane rather than forming well-defined pores. [ABSTRACT FROM AUTHOR]

Simple Summary: Maternal adipose tissue grows during pregnancy to secure the fetus's nutritional supply, and too much visceral adipose tissue at the start of pregnancy can increase metabolic risk and gestational problems. The distribution of fat, and more particularly the rise in visceral fat or central obesity, has been found to be more closely linked to the onset of cardiovascular disease and metabolic syndrome than obesity itself. Our goal is to examine the association between the thickness of the mother's visceral fat, as determined by a first trimester ultrasound exam, and the risk of poor pregnancy outcomes. We observed that women who experienced complications during pregnancy had greater levels of maternal visceral fat, especially gestational diabetes, which was linked to metabolic risk factors including insulin resistance and arterial blood pressure. This fact may imply that the risk of complications would increase more when the distribution of visceral fat (associated with metabolic risk) is greater than expected for a given degree of obesity/body mass index. Obese women are more likely to experience pregnancy complications. The distribution of fat, and more particularly the rise in visceral fat, is well established to be more closely linked to the onset of cardiovascular disease and metabolic syndrome than obesity itself. We aim to examine the relationship between maternal visceral fat assessment in the first trimester and the appearance of adverse pregnancy outcomes. A prospective cohort study including 416 pregnant women was conducted. During the first trimester scan (11–13 + 6 weeks), all individuals had their visceral fat and subcutaneous thicknesses measured by ultrasonography. Blood samples were obtained, and maternal demographics and clinical information were documented. After delivery, the obstetric outcomes were evaluated. We contrasted two groups: one with healthy pregnancies and the other with adverse pregnancy outcomes (APO), defined as the development of at least one of the following complications: gestational diabetes mellitus, hypertensive disorders of pregnancy, abnormal fetal growth, preterm delivery or preterm premature rupture of membranes. Median maternal age was 33 and 34 years old for the uncomplicated and adverse pregnancy outcomes groups, respectively. We found that women with adverse pregnancy outcomes had higher VFT (median 30 vs. 26.5 mm, p = 0.001) and SFT (median 18.9 vs. 17.1 mm, p = 0.03). However, the visceral/subcutaneous fat ratio was not statistically different between groups. Finally, we performed a subanalysis for metabolic and placental vascular dysfunction complications. After performing a multivariate logistic regression analysis adjusted for maternal age, smoking, and mean arterial pressure, both the VFT (aOR 1.03, p < 0.001) and the ratio of visceral/subcutaneous fat (aOR 1.37, p = 0.04) were significantly associated with the development of adverse pregnancy outcomes; however, the associations of VFT and the VFT-to-SFT ratio were higher for the occurrence of gestational diabetes (aOR 1.07, p < 0.001; aOR 2.09, p = 0.001; respectively) and showed no relationships with placental complications. When conducting a first-trimester ultrasound assessment, sonographers may measure VFT without additional time or cost involved. Identification of pregnant women with increased VFT (>37 mm) may benefit from a close follow-up, especially for the development of gestational diabetes, independent of BMI. [ABSTRACT FROM AUTHOR]

Introduction: Extracorporeal wave lithotripsy (ESWL) is considered a first-line treatment for renal and ureteral stones up to 10–20 mm in diameter. Complications are uncommon, with a reported rate of 0–6% in the literature. Bowel perforation has only been described in a few case reports but requires rapid diagnosis and treatment. Methods: A review of the literature from PubMed/Medline, Embase, Cochrane, and Web of Science databases was performed including studies reporting bowel perforation secondary to ESWL between January 1990 and June 2022. Results: We found 16 case reports of intestinal perforation in the literature. Although some patients had previously undergone abdominal surgery or had inflammatory intestinal disease, others were without comorbidities that could lead to complications. Abdominal pain was the main symptom and imaging was required to confirm the diagnosis, which usually necessitated a surgical intervention. As regards the ESWL technique, it appears that the combination of a high energy level and the prone position constitutes a risk factor for these rare complications. At the authors' centre, only one case has been reported among 24,000 ESWL procedures over 20 years: A 59-year-old female who underwent ESWL for a distal right ureteral stone presented acute abdominal pain and free intraperitoneal pelvic fluid on ultrasound. A CT scan revealed a small bowel perforation requiring open laparotomy with primary closure. Conclusions: In conclusion, although bowel perforation after ESWL is rare, progressive abdominal pain with tenderness at physical examination requires proper imaging evaluation to exclude bowel perforation and prompt intervention if required. [ABSTRACT FROM AUTHOR]

The Arabidopsis PROSCOOP genes belong to a family predicted to encode secreted pro-peptides, which undergo maturation steps to produce peptides named SCOOP. Some of them are involved in defence signalling through their perception by a receptor complex including MIK2, BAK1 and BKK1. Here, we focused on the PROSCOOP10 gene, which is highly and constitutively expressed in aerial organs. The MS/MS analyses of leaf apoplastic fluids allowed the identification of two distinct peptides (named SCOOP10#1 and SCOOP10#2) covering two different regions of PROSCOOP10. They both possess the canonical S-X-S family motif and have hydroxylated prolines. This identification in apoplastic fluids confirms the biological reality of SCOOP peptides for the first time. NMR and molecular dynamics studies showed that the SCOOP10 peptides, although largely unstructured in solution, tend to assume a hairpin-like fold, exposing the two serine residues previously identified as essential for the peptide activity. Furthermore, PROSCOOP10 mutations led to an early-flowering phenotype and increased expression of the floral integrators SOC1 and LEAFY, consistent with the de-regulated transcription of PROSCOOP10 in several other mutants displaying early- or late-flowering phenotypes. These results suggest a role for PROSCOOP10 in flowering time, highlighting the functional diversity within the PROSCOOP family. [ABSTRACT FROM AUTHOR]

Pseudopterygium is a non-progressive conjunctival adhesion to the peripheral cornea secondary to a corneal-limbus damage. According to the literature, the main etiology is a previous eye trauma. Nevertheless, this could be biased by the existence of other underdiagnosed causes of pseudopterygium, some of which may have severe consequences for the integrity of the eye and patient's life. This comprehensive literature review was performed based on a search on the PubMed and Google Scholar databases of relevant pseudopterygium published papers according to our current knowledge and seeks to gather the existing evidence about its diverse etiologies and clinical features, as well as to propose a diagnostic algorithm to simplify its correct approach. [ABSTRACT FROM AUTHOR]

Background: Health-related quality of life (HRQoL) allows knowing the subject's feelings in distress and well-being, as well as perception of current and future health. Objective: To assess associations between health-related quality of life, fitness status, and adherence to the Mediterranean diet in Spanish children and adolescents. Methods: A cross-sectional study was carried out in a sample of 305 (47.2% women) children and adolescents aged between 8 and 16 years, in the primary and secondary schools of the province of Ávila (central Spain) (NCT05380674). Subjects were classified according to their quality of life: group 1 (highest quality of life) > group 2 (medium quality of life) > group 3 (lowest quality of life). Results: More participants in group 1 showed higher adherence to Mediterranean diet (70.8%) than other groups (group 2: 55.0%; group 3: 43.4%). It was less likely to find optimal levels of muscle strength as quality of life decreased (OR; 95% CI: group 2: 0.535; 0.303–0.955; and group 3: 0.424; 0.234–0.768). Similar trends were found for speed and agility, but only group 3 showed significant results (OR; 95% CI: group 3: 0.297; 0.162–0.545). Flexibility was also the worst in groups 2 and 3 (OR; 95% CI: G2: 0.403; 0.213–0.762; and group 3: 0.520; 0.282–0.958). Conclusion: High fitness status and adherence to the Mediterranean diet were associated with improved health-related quality of life in schoolchildren of central Spain. [ABSTRACT FROM AUTHOR]

This paper deals with the seismic column design of 35 elevated RC water storage tanks. Tanks comprise a top conic trunk reservoir, a column with variable hollow square cross-sections, and a shallow foundation on a sand layer. The five-column heights considered are 20, 25, 30, 35, and 40 m. The five tanks are subjected to seven degrees of seismic loading characterized by the reference peak ground acceleration in Eurocode 8. The elevated tanks are designed against the full prescriptions of Eurocode 2, Eurocode 8, and the Spaniard Structural Code of Practice. This includes variable loads for seismicity, wind, snow, etc., together with the action of self-weight and dead loads. The optimization design method considered is a variant of the old bachelor algorithm, an adaptive threshold acceptance method with a neighborhood move based on the mutation operator from genetic algorithms. Column results show the high nonlinearity of the problem since the horizontal seismic forces depend on the rigidity and height of the columns. The main features of the optimized tanks give guidance for the practical design of this kind of elevated RC water tank. [ABSTRACT FROM AUTHOR]

(1) Background: New pulse modulation (PM) technologies in Holmium:YAG lasers are available for urinary stone treatment, but little is known about them. We aim to systematically evaluate the published evidence in terms of their lithotripsy performance. (2) Methods: A systematic electronic search was performed (MEDLINE, Scopus, and Cochrane databases). We included all relevant publications, including randomized controlled trials, non-randomized comparative and non-comparative studies, and in-vitro studies investigating Holmium:YAG lithotripsy performance employing any new PM. (3) Results: Initial search yielded 203 studies; 24 studies were included after selection: 15 in-vitro, 9 in-vivo. 10 In-vitro compared Moses with regular PM, 1 compared Quanta's, 1 Dornier MedTech's, 2 Moses with super Thulium Fiber Laser, and 1 compared Moses with Quanta PMs. Six out of seven comparative studies found a statistically significant difference in favor of new-generation PM technologies in terms of operative time and five out of six in fragmentation time; two studies evaluated retropulsion, both in favor of new-generation PM. There were no statistically significant differences regarding stone-free rate, lasing and operative time, and complications between Moses and regular PM when data were meta-analyzed. (4) Conclusions: Moses PM seems to have better lithotripsy performance than regular modes in in-vitro studies, but there are still some doubts about its in-vivo results. Little is known about the other PMs. Although some results favor Quanta PMs, further studies are needed. [ABSTRACT FROM AUTHOR]

Pompe disease (PD) is a rare disorder caused by mutations in the acid alpha-glucosidase (GAA) gene. Most gene therapies (GT) partially rely on the cross-correction of unmodified cells through the uptake of the GAA enzyme secreted by corrected cells. In the present study, we generated isogenic murine GAA-KO cell lines resembling severe mutations from Pompe patients. All of the generated GAA-KO cells lacked GAA activity and presented an increased autophagy and increased glycogen content by means of myotube differentiation as well as the downregulation of mannose 6-phosphate receptors (CI-MPRs), validating them as models for PD. Additionally, different chimeric murine GAA proteins (IFG, IFLG and 2G) were designed with the aim to improve their therapeutic activity. Phenotypic rescue analyses using lentiviral vectors point to IFG chimera as the best candidate in restoring GAA activity, normalising the autophagic marker p62 and surface levels of CI-MPRs. Interestingly, in vivo administration of liver-directed AAVs expressing the chimeras further confirmed the good behaviour of IFG, achieving cross-correction in heart tissue. In summary, we generated different isogenic murine muscle cell lines mimicking the severe PD phenotype, as well as validating their applicability as preclinical models in order to reduce animal experimentation. [ABSTRACT FROM AUTHOR]

Despite the remarkable similarity in amino acid composition, many anticancer peptides (ACPs) display significant differences in terms of activity. This strongly suggests that particular relative dispositions of amino acids (motifs) play a role in the interaction with their biological target, which is often the cell membrane. To better verify this hypothesis, we intentionally modify HB43, an ACP active against a wide variety of cancers. Sequence alignment of related ACPs by ADAPTABLE web server highlighted the conserved motifs that could be at the origin of the activity. In this study, we show that changing the order of amino acids in such motifs results in a significant loss of activity against colon and breast cancer cell lines. On the contrary, amino acid substitution in key motifs may reinforce or weaken the activity, even when the alteration does not perturb the amphipathicity of the helix formed by HB43 on liposomes mimicking their surface. NMR and MD simulations with different membrane models (micelles, bicelles, and vesicles) indicate that the activity reflects the insertion capability in cancer-mimicking serine-exposing membranes, supported by the insertion of N-terminal phenylalanine in the FAK motif and the anchoring to the carboxylate of phosphatidylserine by means of arginine side chains. [ABSTRACT FROM AUTHOR]

It is shown how the decoding algorithms of Pellikaan and Rosenthal can be coupled to produce a decoding algorithm for convolutional codes. Bounds for the computational cost per decoded codeword are also computed. As a case study, our results are applied to a family of convolutional codes constructed by Rosenthal–Schumacher–York and, in this situation, the previous bounds turn out to be polynomial on the degree of the code. [ABSTRACT FROM AUTHOR]

The aim of this study was to develop and validate a scale for measuring the different areas of competence of the social and emotional learning model in students in compulsory secondary education. For this purpose, following the recommendations and quality standards established by the specialised literature, the Social and Emotional Learning Scale was constructed and administered to a convenience sample of 1385 students in compulsory secondary education. The results reveal adequate reliability of the instrument and fairly satisfactory fit indices for the structural model proposed. In addition, external evidence is provided for the validity of the instrument with the variables life satisfaction and school performance. The Social and Emotional Learning Scale is of satisfactory metric quality and provides a detailed and consolidated picture of adolescents' level of self-awareness, social awareness, self-control, interpersonal skills and responsible decision making, which provides students with an instrument that can help to understand their interpersonal and intrapersonal skills and to establish mechanisms for their improvement. [ABSTRACT FROM AUTHOR]

There is a growing interest in the development of sensitive, portable, and low-cost instrumentation for optical chemical (bio)sensing. Such instrumentation can allow real-time decision-making for industry, farmers, and researchers. The combination of optical fiber schemes, luminescence spectroscopy techniques, and new materials for sensor immobilization has allowed the growth of optical sensors. This article focuses on the development of low-cost optoelectronic instrumentation and measurement strategies for optical chemical (bio)sensing. Most of the articles in this field have focused on the chemical sensors themselves, although few have covered the design process for optoelectronic instrumentation. This article tries to fill this gap by presenting designs for real applications, as carried out by the authors. We also offer an introduction to the optical devices and optical measurement techniques used in this field to allow a full understanding of the applications. [ABSTRACT FROM AUTHOR]

The perception and identification of visual stimuli from the environment is a fundamental capacity of autonomous mobile robots. Current deep learning techniques make it possible to identify and segment objects of interest in an image. This paper presents a novel algorithm to segment the object's space from a deep segmentation of an image taken by a 3D camera. The proposed approach solves the boundary pixel problem that appears when a direct mapping from segmented pixels to their correspondence in the point cloud is used. We validate our approach by comparing baseline approaches using real images taken by a 3D camera, showing that our method outperforms their results in terms of accuracy and reliability. As an application of the proposed algorithm, we present a semantic mapping approach for a mobile robot's indoor environments. [ABSTRACT FROM AUTHOR]

This paper presents the analytical method capacity of the upper bound theorem, under modular approach, to extend its application possibilities. Traditionally, this method has been applied in forging processes, considering plane strain condition and parts with double symmetry configuration. However, in this study, the double symmetry is eliminated by means of a fluency plane whose position comes from the center of mass calculated. The study of the load required to ensure the plastic deformation will be focus on the profile of the part, independently on both sides of the fluence plane, modifying the number and the shape of the modules that form the two halves in which the part is defined. This way, it is possible to calculate the necessary load to cause the plastic deformation, whatever its geometric profile. [ABSTRACT FROM AUTHOR]

The application of incremental processes in the manufacturing industry is having a great development in recent years. The first stage of an Incremental Forming Process can be defined as an indentation. Because of this, the indentation process is starting to be widely studied, not only as a hardening test but also as a forming process. Thus, in this work, an analysis of the indentation process under the new Modular Upper Bound perspective has been performed. The modular implementation has several advantages, including the possibility of the introduction of different parameters to extend the study, such as the friction effect, the temperature or the hardening effect studied in this paper. The main objective of the present work is to analyze the three hardening models developed depending on the material characteristics. In order to support the validation of the hardening models, finite element analyses of diverse materials under an indentation are carried out. Results obtained from the Modular Upper Bound are in concordance with the results obtained from the numerical analyses. In addition, the numerical and analytical methods are in concordance with the results previously obtained in the experimental indentation of annealed aluminum A92030. Due to the introduction of the hardening factor, the new modular distribution is a suitable option for the analysis of indentation process. [ABSTRACT FROM AUTHOR]

Focusing on incremental bulk metal forming processes, the indentation process is gaining interest as a fundamental part of these kinds of processes. This paper presents the analysis of the pressure obtained in indentation under the influence of different punch geometries. To this end, an innovative Upper Bound Theorem (UBT) based solution is introduced. This new solution can be easily applied to estimate the necessary force that guarantees plastic deformation by an indentation process. In this work, we propose an accurate analytical approach to analyse indentation under different punches. The new Modular Upper Bound (MUB) method presents a simpler and faster application. Additionally, its complexity is not considerably increased by the addition of more Triangular Rigid Zones. In addition, a two-dimensional indentation model is designed and implemented using the Finite Element Method (FEM). The comparison of the two methods applied to the indentation process analysed--the new Modular Upper Bound technique and the Finite Element Method--reveal close similarities, the new Modular Upper Bound being more computationally efficient. [ABSTRACT FROM AUTHOR]

An electro-quasistatic analysis of an induction micromotor has been realized by using the Cell Method. We employed the direct Finite Formulation (FF) of the electromagnetic laws, hence, avoiding a further discretization. The Cell Method (CM) is used for solving the field equations at the entire domain (2D space) of the micromotor. We have reformulated the field laws in a direct FF and analyzed physical quantities to make explicit the relationship between magnitudes and laws. We applied a primal-dual barycentric discretization of the 2D space. The electric potential has been calculated on each node of the primal mesh using CM. For verification purpose, an analytical electric potential equation is introduced as reference. In frequency domain, results demonstrate the error in calculating potential quantity is neglected (<3‰). In time domain, the potential value in transient state tends to the steady state value. [ABSTRACT FROM AUTHOR]

Job demands are factors that are associated with a physical and psychological cost when it comes to coping with them, but which can also positively affect the motivational process. Demands such as overload, defined as an excessive workload, have not presented positive results in any of the studies that have related it to employee engagement. The present study aims to delve into the possible positive effect of this demand on the intellectual bonding of employees. It is hypothesized that: (a) Initially, the increase in the perception of work overload will show a negative influence on the intellectual engagement of the employees; but (b) high perceptions of overload will change this effect, producing an increase in the intellectual dimension of engagement. The sample is made up of 706 employees of a Spanish multinational company. The results support this asymmetric curvilinear influence. The level of intellectual engagement is significantly reduced when the role overload increases from the lower values of the scale. However, upon reaching high levels of role overload, the intellectual engagement response begins to grow. These results challenge the conceptualization of overload as only negative and opens the door to consider that the positive response to a demand can also occur at high levels of it. [ABSTRACT FROM AUTHOR]

The irruption of information and communication technologies has brought about an abrupt change in the demands placed on future professionals. In this sense, in recent years, information competencies have gained importance in university education from a cross-cutting perspective, which advocates as its main purpose the training of young people in information search, evaluation, processing and communication skills, especially through a digital network. Based on this idea, the present work aims to develop the Information Competence Scale for Future Teachers (ICS-FT), in order to measure the level of self-perceived skills in this area. For this purpose, a work design is presented below concerning an empirical validation, divided into different phases: the validation of content, construct and reliability, which has taken as a pilot sample a total of 259 university students studying the degree of Primary Education. The results of the validation determined the optimal conditions of content, construct and reliability that allowed the application of this scale as a generic approach to determine the level of competence in the information skills of future teachers. [ABSTRACT FROM AUTHOR]

This paper presents the results of a comparative evaluation of the tensile strength behaviors of parts obtained by additive manufacturing using fused filament fabrication (FFF) technology. The study investigated the influences of the deposition printing parameters for both polymers and fiber-reinforced polymers. Polymeric materials that are widely used in FFF were selected, including acrylonitrile butadiene styrene (ABS), polylactic acid (PLA), and nylon. Carbon and glass continuous fibers were used to reinforce the nylon matrix in composite materials. The study utilized two manufacturing methods. Polymers were manufactured using an Ultimaker 2 Extended+ device and the fiber-reinforced polymer specimens were obtained using a Markforged Mark Two printer. The entire set of specimens was eventually subjected to destructive monoaxial tensile tests to measure their responses. The main goal of this study was to estimate the effect of the different infill patterns applied (zig-zag, concentric, and four different orientations lines) on the mechanical properties of pure thermoplastic materials and reinforced polymers. Results show a spectacular increase in the tensile stress at break, which for polymers reaches an average value of 27.53 MPa compared to 94.51 MPa in the case of composites (increase of 70.87%). A similar increase occurs in the case of tensile stress at yield with values of 31.87 MPa and 105.98 MPa, respectively, which represents an increase of 69.93%. The influence of the infill of the fiber is decisive, reaching, in the 0-0 arrangement, mean values of 220.18 MPa for tensile stress at break and 198.26 MPa for tensile stress at yield. [ABSTRACT FROM AUTHOR]

Brain–computer interfaces (BCI) are a type of assistive technology that uses the brain signals of users to establish a communication and control channel between them and an external device. BCI systems may be a suitable tool to restore communication skills in severely motor-disabled patients, as BCI do not rely on muscular control. The loss of communication is one of the most negative consequences reported by such patients. This paper presents a BCI system focused on the control of four mainstream messaging applications running in a smartphone: WhatsApp, Telegram, e-mail and short message service (SMS). The control of the BCI is achieved through the well-known visual P300 row-column paradigm (RCP), allowing the user to select control commands as well as spelling characters. For the control of the smartphone, the system sends synthesized voice commands that are interpreted by a virtual assistant running in the smartphone. Four tasks related to the four mentioned messaging services were tested with 15 healthy volunteers, most of whom were able to accomplish the tasks, which included sending free text e-mails to an address proposed by the subjects themselves. The online performance results obtained, as well as the results of subjective questionnaires, support the viability of the proposed system. [ABSTRACT FROM AUTHOR]

The main objective of this article has been to evaluate the effect that the implementation of the EXPLORIA project has had on the Engineering Degree in Industrial Design and Product Development. The EXPLORIA project aims to develop an integrated competence map of the learning process, where the subjects are no longer considered as isolated contents, by elaborating an integrated learning process where the competences and learning outcomes of the subjects are considered as a whole, global and comprehensive learning. The EXPLORIA project connects the competencies of the different STEAM subjects that make up the degree, designing a learning process as a logical, sequential and incremental itinerary. Through concepts on which the foundations of design are based—shape, volume, colour, space and structure—the competencies of the different subjects are defined in incremental learning levels: understanding, applying, experimenting and developing, all taken from Bloom's taxonomy. Mathematics is linked to the rest of learning through active learning methodologies that make learning useful. This new methodology changes the student's affective domain towards mathematics in which positive emotions are transformed into positive attitudes that will improve the learning result and therefore, the students' academic results. To validate it, at the end of the paper, the academic results compared with previous years are shown, as well as an ad hoc survey of the students' assessment of the new teaching methodology. [ABSTRACT FROM AUTHOR]

In modern education nowadays, the use of game-based learning as a teaching and learning method is popular in all school subjects, including mathematics. There are numerous studies dealing with the influences of this teaching method on the students' achievements. Modern teaching theories consider an important effect of education on the development of students' affective domain, connected with the subject and its teaching. In this work, the author studies journal articles that the use game-based learning in mathematics to assess its effects on the students, with the aim to analyze its impact on students' affective domain. To achieve this, a systematic review with the use of a PRISMA statement is applied. The data sources are 57 journal articles from the area of interest listed in the Web of Sciences and Scopus. The results indicate that 54% of the articles consider the affective domain in the measurement of the effects of game-based learning in mathematics education. These articles report mostly (84%) the positive influences of game-based learning on students' motivation, engagement, attitudes, enjoyment, state of flow, etc. The rest of the articles show mixed results, with the authors' conclusions possibly affected by flaws in the research instruments, selection of study groups, and game design, therefore, stressing the importance of these elements in future research on this topic. [ABSTRACT FROM AUTHOR]

This document reports how formative assessment strategies promote the knowledge of modeling of pre-service mathematics teachers. This knowledge is understood from content and vehicle points of view. Formative assessment strategies were designed and experimented with 14 participants in a mathematical modeling course offered to pre-service teachers in a Colombian university. Thematic analysis was conducted on lesson plans built by pre-service teachers. In those plans, they evinced knowledge of class management, mathematics teaching, problem solving, and modeling teaching. Finally, the collective construction of assessment rubrics is highlighted. Its contributions and limitations as a formative assessment tool are reported. The role played by the advisors' feedback and support to pre-service teachers is also presented. [ABSTRACT FROM AUTHOR]

In this paper, we develop a review of the research focused on the teaching and learning of ordinary differential equations with the following three purposes: to get an overview of the existing literature of the topic, to contribute to the integration of the actual knowledge, and to define some possible challenges and perspectives for the further research in the topic. The methodology we followed is a combination of a systematic literature review and a bibliometric analysis. The contributions of the paper are given by the following: shed light on the latest research in this area, present a characterization of the actual research lines regarding the teaching and learning of ordinary differential equations, present some topics to be addressed in the next years and define a starting point for researchers who are interested in developing research in this field. [ABSTRACT FROM AUTHOR]

Hepatitis B (HBV) is a pathogen virus with transmission mechanisms that include contact with the infected blood or bodily fluids of the infected organism. Nowadays, healthcare workers are one of the most exposed groups to HBV. Conventionally, completing a vaccine series dosage with Engerix B® lowers this risk by providing workers with immunity to the virus. However, through the years, we have encountered nonresponsive health personnel to the Engerix B® vaccine; hence, the Occupational Health Service of Poniente Hospital studied the Fendrix® adjuvanted vaccine as an alternative vaccine to develop immunological responses in healthcare workers who do not respond to vaccination with Engerix B®. In our study, we employed a vaccination schedule with the Fendrix® vaccine, performing serology tests on the cases after the application of each dose. The results obtained showed humoral immunity in 92.3% of the cases, with a remarkable increase in antibody titer after the first doses. These encouraging results support the future inclusion of this vaccine as one possible alternative for the immunization to HBV for healthcare workers nonresponsive to Engerix B®. [ABSTRACT FROM AUTHOR]

Spatial intelligence is an essential skill for understanding and solving real-world problems. These visuospatial skills are fundamental in the learning of different Science, Technology, Engineering and Mathematics (STEM) subjects, such as Technical Drawing, Physics, Robotics, etc., in order to build mental models of objects or graphic representations from algebraic expressions, two-dimensional designs, or oral descriptions. It must be taken into account that spatial intelligence is not an innate skill but a dynamic skill, which can be enhanced by interacting with real and/or virtual objects. This ability can be enhanced by applying new technologies such as augmented reality, capable of illustrating mathematical procedures through images and graphics, which help students considerably to visualize, understand, and master concepts related to mathematical functions. The aim of this study is to find out whether the integration of the Geogebra AR (Augmented Reality) within a contextualized methodological environment affects the academic performance and spatial skills of fourth year compulsory secondary education mathematics students. [ABSTRACT FROM AUTHOR]

Esophageal cancer is an aggressive lethal malignancy causing thousands of deaths every year. While current treatments have poor outcomes, cecropinXJ (CXJ) is one of the very few peptides with demonstrated in vivo activity. The great interest in CXJ stems from its low toxicity and additional activity against most ESKAPE bacteria and fungi. Here, we present the first study of its mechanism of action based on molecular dynamics (MD) simulations and sequence-property alignment. Although unstructured in solution, predictions highlight the presence of two helices separated by a flexible hinge containing P24 and stabilized by the interaction of W2 with target biomembranes: an amphipathic helix-I and a poorly structured helix-II. Both MD and sequence-property alignment point to the important role of helix I in both the activity and the interaction with biomembranes. MD reveals that CXJ interacts mainly with phosphatidylserine (PS) but also with phosphatidylethanolamine (PE) headgroups, both found in the outer leaflet of cancer cells, while salt bridges with phosphate moieties are prevalent in bacterial biomimetic membranes composed of PE, phosphatidylglycerol (PG) and cardiolipin (CL). The antibacterial activity of CXJ might also explain its interaction with mitochondria, whose phospholipid composition recalls that of bacteria and its capability to induce apoptosis in cancer cells. [ABSTRACT FROM AUTHOR]

Public procurement has recently taken into account social and sustainable factors, increasing academic literature in this field. The aim of this paper is to map the intellectual structure of social and sustainable public procurement research by means of delimiting the scientific domain's research traditions, its disciplinary composition, and influential research topics. Given that there is a literature gap in bibliometric studies applied to this field, we conducted a co-citation analysis to identify the intellectual structure of this area of knowledge. Co-citation analyses identify networks of interconnections and, consequently, detect the most (and the least) active research areas, being a good complement to traditional literature reviews. This article contributes to science development because it is the first paper to carry out a bibliometric analysis in the field of social and sustainable public procurement, as well as the first one to conduct a co-citation analysis among public procurement research. Consequently, it is also the first article to detect which papers have become burst in this research field. The results show twelve different clusters of publications that were cited by researchers who wrote papers on social and sustainable public procurement. In other words, the sources of knowledge that scholars used as references are analysed, identifying papers that can be considered turning points, as well as those that became specially cited over a discrete period of time. Six different research trends were identified over the last decade in regard to social and sustainable public procurement research. The conclusions highlight the relevance of the findings, especially because they provide guidance to researchers when conducting literature reviews, given that the most significant journals and papers are identified. [ABSTRACT FROM AUTHOR]

K11 is a synthetic peptide originating from the introduction of a lysine residue in position 11 within the sequence of a rationally designed antibacterial scaffold. Despite its remarkable antibacterial properties towards many ESKAPE bacteria and its optimal therapeutic index (320), a detailed description of its mechanism of action is missing. As most antimicrobial peptides act by destabilizing the membranes of the target organisms, we investigated the interaction of K11 with biomimetic membranes of various phospholipid compositions by liquid and solid-state NMR. Our data show that K11 can selectively destabilize bacterial biomimetic membranes and torque the surface of their bilayers. The same is observed for membranes containing other negatively charged phospholipids which might suggest additional biological activities. Molecular dynamic simulations reveal that K11 can penetrate the membrane in four steps: after binding to phosphate groups by means of the lysine residue at the N-terminus (anchoring), three couples of lysine residues act subsequently to exert a torque in the membrane (twisting) which allows the insertion of aromatic side chains at both termini (insertion) eventually leading to the flip of the amphipathic helix inside the bilayer core (helix flip and internalization). [ABSTRACT FROM AUTHOR]

Currently, the use of technology has become one of the most popular educational trends in Higher Education. One of the most popular methods on the Higher Education stage is the Flipped Classroom, characterised by the use of both face-to-face and virtual teaching through videos and online material, promoting more autonomous, flexible and dynamic teaching for students. In this work, we started to compile the main articles that used Flipped Classroom within the mathematical area in Higher Education, with the aim of analysing their main characteristics, as well as the impact caused on students. To do so, the method of systematic review was used, focusing on those empirical experiences published in Web of Sciences and Scopus. The results indicated that, in most cases, the implementation of Flipped Classroom led to an improvement in students' knowledge and attitudes towards mathematical content and discipline. In addition, aspects such as collaborative work, autonomy, self-regulation towards learning or academic performance were benefited through this method. [ABSTRACT FROM AUTHOR]

The existence of ethical and deontological codes is a reality integrated in highly competitive sectors, such as the medical sector or the legal profession. Most studies on ethics and deontology focus on the way in which they are communicated and their effectiveness. However, no special interest has been paid to the existence, application and content of ethics and deontology in the university sector and its relationship with the transmission of ethical and deontological principles to students. Professionals, employees and managers who will play their role in society, perform their work in companies or develop strategic plans, are trained at universities, which must play an important role in the ethical and deontological training of future social actors. Therefore, it is necessary to respond to whether public universities have codes of ethics and whether the fact of having these codes implies a greater commitment to the inclusion of ethical values in their training programs. To this end, Spanish public universities are analyzed and the results, which are grouped by areas of knowledge, are presented. The results achieved provide useful insight for university managers concerned with implementing ethical and responsible policies. [ABSTRACT FROM AUTHOR]

The objective of this research was two-fold. First, to determine the impact of a Science, Technology, Engineering and Mathematics (STEM) Education program on school performance amongst primary education students. Second, to identify the potential benefits of this program on the key competences of university students in Primary Education Teacher Training. The primary education students' sub-sample, after being matched on key covariates, was randomly assigned either to the experimental (N = 25) or control group (N = 25). The university students' sub-sample consisted of 26 students self-selected from the Primary Education Teacher Training degree. The intervention consisted of 20 two-hour weekly sessions of highly structured after-school tutoring delivered by previously trained university students. Although statistical significance was not reached for the hypotheses tested, notably, the results showed between small and moderate effect sizes (i.e., magnitude and direction of the program impact) for primary education students on core STEM subjects (e.g., mathematics d = 0.29, natural science d = 0.26), and for university students on some key employability competencies (e.g., action orientation d = 0.27 or team orientation d = 0.54). [ABSTRACT FROM AUTHOR]

Ice accretion presents serious safety issues, as airplanes are exposed to supercooled water droplets both on the ground and while flying through clouds in the troposphere. Prevention of icing is a main concern for both developers and users of aircraft. The successful solution of this problem implies the combination of active and passive methods and the use of advanced sensors for early detection of icing and monitoring of ice accretion and de-icing processes. This paper focuses on the development of passive solutions. These include advanced anti-icing coatings deposited by a variety of chemical methods including sol-gel, advanced paints based on polyester combined with fluorinated derivatives and applied by electrostatic spray deposition and conventional silicone-based paints modified by adding alumina nanoparticles. Water contact angle has been measured in all cases, demonstrating the hydrophobic character of the coatings. An ice accretion test has been carried out in a laboratory scale icing wind tunnel (IWT) located in a cold climate chamber. Three different studies have been undertaken: ice accretion measurement, durability of the anti-icing behavior after several icing/de-icing cycles and ice adhesion testing by means of the double lap shear test (DLST) methodology. All the studied coatings have shown significant anti-icing behavior which has been maintained, in some cases, beyond 25 cycles. Although these results are still far from any possible application for aeronautic components, they provide interesting insights for new developments and validate the laboratory scale tests. [ABSTRACT FROM AUTHOR]

Robots have begun to populate the everyday environments of human beings. These social robots must perform their tasks without disturbing the people with whom they share their environment. This paper proposes a navigation algorithm for robots that is acceptable to people. Robots will detect the personal areas of humans, to carry out their tasks, generating navigation routes that have less impact on human activities. The main novelty of this work is that the robot will perceive the moods of people to adjust the size of proxemic areas. This work will contribute to making the presence of robots in human-populated environments more acceptable. As a result, we have integrated this approach into a cognitive architecture designed to perform tasks in human-populated environments. The paper provides quantitative experimental results in two scenarios: controlled, including social navigation metrics in comparison with a traditional navigation method, and non-controlled, in robotic competitions where different studies of social robotics are measured. [ABSTRACT FROM AUTHOR]

In this work we analyse the temperature distribution in a conductor disk in transitory regime. The disk is in motion in a stationary magnetic field generated by a permanent magnet and so, the electric currents induced inside it generate heat. The system acts as a magnetic brake and is analysed using infrared sensor techniques. In addition, for the simulation and analysis of the magnetic brake, a new thermal convective matrix for the 3D Cell Method (CM) is proposed. The results of the simulation have been verified by comparing the numerical results with those obtained by the Finite Element Method (FEM) and with experimental data obtained by infrared technology. The difference between the experimental results obtained by infrared sensors and those obtained in the simulations is less than 0.0459%. [ABSTRACT FROM AUTHOR]