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Hexavalent chromium (Cr[VI]) is a widespread environmental pollutant in air and water that is primarily attributed to industrial pollution. The current maximum contaminant levels (MCLs) for drinking water from the World Health Organization and the U.S. Environmental Protection Agency (0.05 and 0.1 mg/L, respectively) were set based on contact dermatitis and warrant further toxicological investigation. While Cr(VI) is neurotoxic and accumulates in the brain, most animal studies only report whole-brain Cr, leaving large knowledge gaps. Few studies consider differences between ages or sexes, and fewer consider essential metal dyshomeostasis. We sought to investigate where Cr accumulates in the brain, considering sex and age differences, following a 90-day drinking water exposure to current MCLs. Here, we report Cr levels in six brain regions of rats exposed to drinking water Cr(VI). We observed Cr only accumulated in the hippocampus, and only in older females. We further assessed changes to essential metals in the hippocampus, observing opposite effects across sexes and between young rats compared to older rats. In sum, our data indicate drinking water Cr(VI) selectively targeted the hippocampus, with geriatric females accumulating the most Cr, and induced significant essential metal dyshomeostasis even in tissues lacking evident Cr accumulation. [ABSTRACT FROM AUTHOR]

With the increasing utilization of urban underground space, new tunnels frequently intersect with existing tunnels and operational railways. However, sometimes the excavation and unloading of new tunnels can cause deformation of adjacent existing tunnels and railways, significantly affecting their normal operation. We used finite element software to predict the influence of new tunnel construction on overcrossing existing tunnels and down-traversing operational railways by a dynamic tunneling model based on a connection channel project of the east and west squares of a railway station. This article is not only control the distance between the two tunnels, but the new tunnel and the existing tunnel, as well as the new tunnel and the operation of the railway, the positional relationship between the three, the deformation laws of existing tunnels and operational railways during the construction of new tunnels with different buried depths are analyzed. The results show that the deformation curves of existing tunnels and operational railways present a normal distribution. The maximum deformation position is at the intersection with the new tunnel upon completion of the new tunnel excavation construction. Moreover, an increase in the buried depth of the new tunnel increases the deformation of the operational railway and the existing tunnel. The influence of the depth change of the new tunnel on the settlement of the operational railway is greater than that of the existing tunnel. [ABSTRACT FROM AUTHOR]

We are facing a critical aging crisis, with geriatric populations (65+) growing to unprecedented proportions and ~4 million people (a 6.5-fold increase) expected to become centenarians by 2050. This is compounded by environmental pollution, which affects individuals of all ages and contributes to age-related diseases. As we have a limited understanding of how environmental pollutants affect older populations distinctly from younger populations, these longer-lived geriatric populations present a key knowledge gap. To address this knowledge gap, we employ a "Toxic Aging Coin" approach: heads consider how age impacts chemical toxicity, and tails consider how chemicals act as gerontogens—or how they accelerate biological aging. We employed this approach to investigate hexavalent chromium (Cr[VI]) impacts on female rats exposed to 0.05 or 0.1 mg Cr(VI)/L in drinking water for 90 days; these are the maximum contaminant levels (i.e., the highest levels permitted) from the World Health Organization and U.S. Environmental Protection Agency, respectively. During exposure, rats performed a battery of behavior assays to assess grip strength, locomotor coordination, anxiety, spatial memory, sociability, and social novelty preference. We observed age differences in Cr(VI) neurotoxicity, with grip strength, locomotor function, and spatial memory in middle-aged females being particularly affected. We further compared these results in females to results in males, noting many sex differences, especially in middle-aged rats. These data emphasize the need to consider age and sex as variables in toxicology and to revisit drinking water regulations for Cr(VI). [ABSTRACT FROM AUTHOR]

Permanent magnet motors (PMMs) have emerged as key components in numerous industrial applications due to their high efficiency, compact size, and robust performance characteristics. However, to attain optimal performance in PMM drives, accurately predicting and mitigating core losses is paramount. This paper aims to provide a comprehensive review of advancements and methodologies for enhancing the performance of PMM drives by integrating equivalent circuit models (ECMs) that account for core losses. Firstly, the significance of core losses in motor drives is underscored, alongside a survey of research endeavors dedicated to core loss reduction. Notably, emphasis is placed on mathematical models offering both swift computation and reasonable accuracy. Subsequently, the paper delves into the development of ECMs, focusing on approaches adept at capturing core loss effects across diverse operating conditions. Moreover, this paper explores the utilization of these improved ECMs in the design and control of PMMs to achieve enhanced performance. By integrating core loss considerations into design and control strategies, PMM drives can optimize efficiency, torque production, and overall system performance. In summary, this paper may consolidate the current state-of-the-art techniques for enhancing PMM performance through the integration of core-loss-aware ECMs. It highlights key research directions and opportunities for further advancements in this critical area, aiming to foster the development of more efficient and reliable PMM-based systems for a wide range of industrial applications. [ABSTRACT FROM AUTHOR]

Hexavalent chromium [Cr(VI)] is a known human lung carcinogen with widespread exposure in environmental and occupational settings. Despite well-known cancer risks, the molecular mechanisms of Cr(VI)-induced carcinogenesis are not well understood, but a major driver of Cr(VI) carcinogenesis is chromosome instability. Previously, we reported Cr(VI) induced numerical chromosome instability, premature centriole disengagement, centrosome amplification, premature centromere division, and spindle assembly checkpoint bypass. A key regulator of these events is securin, which acts by regulating the cleavage ability of separase. Thus, in this study we investigated securin disruption by Cr(VI) exposure. We exposed human lung cells to a particulate Cr(VI) compound, zinc chromate, for acute (24 h) and prolonged (120 h) time points. We found prolonged Cr(VI) exposure caused marked decrease in securin levels and function. After prolonged exposure at the highest concentration, securin protein levels were decreased to 15.3% of control cells, while securin mRNA quantification was 7.9% relative to control cells. Additionally, loss of securin function led to increased separase activity manifested as enhanced cleavage of separase substrates; separase, kendrin, and SCC1. These data show securin is targeted by prolonged Cr(VI) exposure in human lung cells. Thus, a new mechanistic model for Cr(VI)-induced carcinogenesis emerges with centrosome and centromere disruption as key components of numerical chromosome instability, a key driver in Cr(VI) carcinogenesis. [ABSTRACT FROM AUTHOR]

Monocytes (Mos) are believed to play important roles during the generation of immune response. In our previous study, CVC1302, a complex of PRRs agonists, was demonstrated to recruit Mo into lymph nodes (LNs) in order to present antigen and secret chemokines (CXCL9 and CXCL10), which attracted antigen-specific CD4+ T cells. As it is known that Mos in mice are divided into two main Mo subsets (Ly6C+ Mo and Ly6C− Mo), we aimed to clarify the CVC1302-recruiting Mo subset and functions in the establishment of immunity. In this study, we found that CVC1302 attracted both Ly6C+ Mo and Ly6C− Mo into draining LNs, which infiltrated from different origins, injection muscles and high endothelial venule (HEV), respectively. We also found that the numbers of OVA+ Ly6C+ Mo in the draining LNs were significantly higher compared with OVA+ Ly6C− Mo. However, the levels of CXCL9 and CXCL10 produced by Ly6C− Mo were significantly higher than Ly6C+ Mo, which plays important roles in attracting antigen-specific CD4+ T cells. Under the analysis of their functions in initiating immune responses, we found that the ability of the Ly6C+ monocyte was mainly capturing and presenting antigens, otherwise; the ability of the Ly6C− monocyte was mainly secreting CXCL9 and CXCL10, which attracted antigen-specific CD4+ T cells through CXCR3. These results will provide new insights into the development of new immunopotentiators and vaccines. [ABSTRACT FROM AUTHOR]

This study found a higher percentage of CD8+ T cells in piglets immunized with a CVC1302-adjuvanted inactivated foot-and-mouth disease virus (FMDV) vaccine. We wondered whether the CVC1302-adjuvanted inactivated FMDV vaccine promoted cellular immunity by promoting the antigen cross-presentation efficiency of ovalbumin (OVA) through dendritic cells (DCs), mainly via cytosolic pathways. This was demonstrated by the enhanced levels of lysosomal escape of OVA in the DCs loaded with OVA and CVC1302. The higher levels of ROS and significantly enhanced elevated lysosomal pH levels in the DCs facilitated the lysosomal escape of OVA. Significantly enhanced CTL activity levels was observed in the mice immunized with OVA-CVC1302. Overall, CVC1302 increased the cross-presentation of exogenous antigens and the cross-priming of CD8+ T cells by alkalizing the lysosomal pH and facilitating the lysosomal escape of antigens. These studies shed new light on the development of immunopotentiators to improve cellular immunity induced by vaccines. [ABSTRACT FROM AUTHOR]

Gray mold infected with Botrytis cinerea frequently appears on fruits and vegetables throughout the supply chain after harvest, leading to economic losses. Biological control of postharvest disease with phytochemicals is a promising approach. CA (cinnamaldehyde) is a natural phytochemical with medicinal and antimicrobial activity. This study evaluated the effect of CA in controlling B. cinerea on fresh pepper fruit. CA inhibited B. cinerea growth in vitro significantly in a dose- (0.1–0.8 mM) and time-dependent (6–48 h) manner, with an EC50 (median effective concentration) of 0.5 mM. CA induced the collapse and breakdown of the mycelia. CA induced lipid peroxidation resulting from ROS (reactive oxygen species) accumulation in mycelia, further leading to cell leakage, evidenced by increased conductivity in mycelia. CA induced mycelial glycerol accumulation, resulting in osmotic stress possibly. CA inhibited sporulation and spore germination resulting from ROS accumulation and cell death observed in spores. Spraying CA at 0.5 mM induced a defense response in fresh pepper fruits, such as the accumulation of defense metabolites (flavonoid and total phenols) and an increase in the activity of defense enzymes (PAL, phenylalanine ammonia lyase; PPO, polyphenol oxidase; POD, peroxidase). As CA is a type of environmentally friendly compound, this study provides significant data on the activity of CA in the biocontrol of postharvest gray mold in peppers. [ABSTRACT FROM AUTHOR]

With the increasing demand for smaller, lighter, and more affordable electromagnetic devices, there is a growing trend toward developing high-power-density transformers and electrical machines. While increasing the operating frequency is a straightforward approach to achieving high power density, it can lead to significant power loss within a limited volume, resulting in excessive temperature rise and device degradation. Therefore, it is crucial to design high-power-density electromagnetic devices that exhibit low power loss and efficient thermal dissipation to address these challenges. Advanced techniques, such as the utilization of novel and advanced electromagnetic materials, hold great promise for overcoming these issues. Specifically, nanocrystalline and amorphous magnetic materials have emerged as highly effective solutions for reducing power loss and increasing efficiency in electromagnetic devices. This paper aims to provide an overview of the application of nanocrystalline and amorphous magnetic materials in transformers and electrical machines, along with key technologies and the major challenges involved. [ABSTRACT FROM AUTHOR]

As we face issues of fossil fuel depletion and environmental pollution, it is becoming increasingly important to transition towards clean renewable energies and electric vehicles (EVs). However, designing electric motors with high power density for EVs can be challenging due to space and weight constraints, as well as issues related to power loss and temperature rise. In order to overcome these challenges, a significant amount of research has been conducted on designing high-power-density electric motors with advanced materials, improved physical and mathematical modeling of materials and the motor system, and system-level multidisciplinary optimization of the entire drive system. These technologies aim to achieve high reliability and optimal performance at the system level. This paper provides an overview of the key technologies for designing high-power-density electric motors for EVs with high reliability and system-level optimal performance, with the focus on advanced magnetic materials and the proper modeling of core losses under two-dimensional or three-dimensional vectorial magnetizations. This paper will also discuss the major challenges associated with designing these motors and the possible future research directions in the field. [ABSTRACT FROM AUTHOR]

Electric motors play a crucial role in modern industrial and domestic applications. With the trend of more and more electric drives, such as electric vehicles (EVs), the requirements for electric motors become higher and higher, e.g., high power density with good thermal dissipation and high reliability in harsh environments. Many efforts have been made to develop high performance electric motors, such as the application of advanced novel electromagnetic materials, modern control algorithms, advanced mathematical modeling, numerical computation, and artificial intelligence based optimization design techniques. Among many advanced magnetic materials, soft magnetic composite (SMC) appears very promising for developing novel electric motors, thanks to its many unique properties, such as magnetic and thermal isotropies, very low eddy current loss, and the prospect of low-cost mass production. This paper aims to present a comprehensive review about the application of SMC for developing various electric motors for electric drives, with emphasis on those with three-dimensional (3D) magnetic flux paths. The major techniques developed for designing the 3D flux SMC motors are also summarized, such as vectorial magnetic property characterization and system-level multi-discipline robust design optimization. Major challenges and possible future work in this area are also discussed. [ABSTRACT FROM AUTHOR]

High-temperature superconductor (HTS) bulks have demonstrated extremely intriguing potential for industrial and commercial applications due to their capability to trap significantly larger magnetic fields than conventional permanent magnets. The magnetic field in electrical rotating machines is a combination of alternating and rotational fields. In contrast, all previous research on the characterization of electromagnetic properties of HTS have solely engrossed on the alternating AC magnetic fields and the associated AC loss. This research paper gives a thorough examination of the AC loss measurement under various conditions. The obtained results are compared to the finite element-based H-formulation. The AC loss is measured at various amplitudes of circular flux density patterns and compared with the AC loss under one-dimensional alternating flux density. The loss variation has also been studied at other frequencies. The findings in this research paper provide more insights into material characterization, which will be useful in the design of future large-scale HTS applications. [ABSTRACT FROM AUTHOR]

Fusarium oxysporum f. sp. niveum (FON) causes watermelon wilt that is one of the major disease-causing yield losses of watermelon. Sustainable development of agriculture requires controlling watermelon wilt disease with good environmental performance. One important approach is to identify environmental-friendly compounds with inhibitory activity against FON. Thymol is a plant-derived compound that is safe for ecology. Little is known about the application of thymol in agriculture. In this study, we studied the inhibitory activity of thymol against FON by using morphological, physiological, and histochemical approaches. Thymol significantly inhibited colony diameter of FON in a dose-dependent manner, with EC50 at 21 µg/mL. Thymol at 10, 21, and 35 µg/mL decreased the fresh weight of FON mycelia by 29.0%, 50.6%, and 69.5%, respectively. Microscopic observation revealed irregular damage and loss of shape of mycelia upon thymol exposure. Thymol induced the accumulation of superoxide radical in mycelial cells and accompanied increased activity of antioxidative enzymes (SOD, superoxide dismutase; CAT, catalase). Thymol induced membrane permeability was indicated by lipid peroxidation and electrolyte leakage (increased by 29–58%) in mycelial cells. These results suggested that thymol induced oxidative damage in mycelia, which may be one of the possible reasons for thymol-induced mycelial cell death observed with fluorescent detection. Thymol decreased the production of conidia and inhibited the germination of conidia. Thymol induced superoxide radical accumulation, lipid peroxidation, and cell death in conidia as well. All of these results revealed the inhibitory activity of thymol against FON, which may have resulted from the superoxide radical-induced oxidative injury in both conidia and mycelia of FON. [ABSTRACT FROM AUTHOR]

The magnetic properties of magnetic cores are essential for the design of electrical machines, and consequently appropriate mathematical modeling is needed. Usually, the design and analysis of electrical machines consider only the one-dimensional (1D) magnetic properties of core materials, i.e., the relationship of magnetic flux density (B) versus magnetic field strength (H), and their associated power loss under 1D magnetization, in which the B and H are constrained in the same orientation. Some studies have also been performed with the two-dimensional (2D) magnetizations in which the B and H are vectorial, rotating on a plane, and they may not be in the same direction. It has been discovered that the 2D rotational property is very different from its 1D alternating counterpart. However, the magnetic fields in an electrical machine, in particular claw pole and transverse flux machines, are naturally three-dimensional (3D), and the B and H vectors are rotational and may not lie on the same plane. It can be expected that the 3D vectorial property might be different from its 2D or 1D counterpart, and hence it should be investigated for the interests of both academic research and engineering application. This paper targets at a general summary about the magnetic material characterization with 3D vectorial magnetization, and their application prospect in electrical machine design and analysis. [ABSTRACT FROM AUTHOR]

Thymol is a plant-derived natural compound with antimicrobial activity. However, we have little knowledge about the application of thymol in agriculture. One of the limitations is the high volatility and low aqueous solubility of thymol. Tomato gray mold caused by Botrytis cinerea is one of the most devastating postharvest diseases. In this study, we prepared a nano-emulsion of thymol (named as Nano-Thy) to form a stable O/W (oil in water) microemulsion. In vitro experiments showed that Nano-Thy had antifungal activity against B. cinerea by inhibiting mycelial growth and spore germination. Nano-Thy induced ROS accumulation in mycelia, further leading to lipid peroxidation, cell membrane damage, and subsequent cell death. Nano-Thy significantly prevented the infection of B. cinerea on fresh tomato fruits. Finally, we discussed the mechanisms and their significance in controlling postharvest disease of fruit crops. [ABSTRACT FROM AUTHOR]

Over time, high-temperature superconductor (HTS)-coated conductors (CCs) have proven to be promising candidates for future high-efficiency and high-power density electrical machines. However, their commercialization is handicapped due to the AC dissipative loss that occurs upon exposure to external magnetic fields. In rotating electromagnetic devices, the external magnetic field is a combination of alternating and rotating magnetic fields. Most of the research is devoted to the effect of exposure of the superconductors to alternating magnetic fields only. This article presents an investigation to observe the behavior of HTSCCs under rotating magnetic fields, particularly the AC loss, using a finite-element-based homogeneous H-formulation technique. Our investigation shows that the AC loss could be considerably high when HTSCCs are exposed to rotating magnetic fields and, ultimately, could affect the cooling efficiency of future high-efficiency and high-power density electrical machines. [ABSTRACT FROM AUTHOR]

High-temperature superconducting materials have shown great potential for the design of large-scale industry applications. However, they are complicated under AC conditions, resulting in penalties such as power loss or AC loss. This loss has to be considered in order to design reliable and efficient superconducting devices. Furthermore, when superconductors are used in rotating machines, they may be exposed to rotating magnetic fields, which is critical for the design of such machines. Existing AC loss measuring techniques are limited to measuring under one-dimensional AC magnetic fields or transport currents. Therefore, it is essential to develop and investigate robust experimental techniques to investigate the loss mechanism in HTS machines. In this paper, a new and novel experimental technique has been presented to measure AC loss in rotating magnetic field conditions. The loss under rotating magnetic fields is measured and compared by numerical modeling methods, and the results show a strong correlation with the numerical modeling and show the effectiveness of the experimental setup. [ABSTRACT FROM AUTHOR]

Amorphous metal (AM), specifically amorphous ferromagnetic metal, is considered as a satisfactory magnetic material for exploring electromagnetic devices with high-efficiency and high-power density, such as electrical machines and transformers, benefits from its various advantages, such as reasonably low power loss and very high permeability in medium to high frequency. However, the characteristics of these materials have not been investigated comprehensively, which limits its application prospects to good-performance electrical machines that have the magnetic flux density with generally rotational and non-sinusoidal features. The appropriate characterization of AMs under different magnetizations is among the fundamentals for utilizing these materials in electrical machines. This paper aims to extensively overview AM property measurement techniques in the presence of various magnetization patterns, particularly rotational magnetizations, and AM property modeling methods for advanced electrical machine design and analysis. Possible future research tasks are also discussed for further improving AM applications. [ABSTRACT FROM AUTHOR]

Phytophthora capsici is one of the devastating pathogens, causing foliar blight, root rot, and fruit rot in peppers. Cinnamaldehyde (CA) is a natural compound coming from Cinnamomum cassia. The medicinal properties of CA have been widely identified. Limited knowledge is known about the application of CA in agriculture. In this study, CA significantly inhibited P. capsici, which further suppressed Phytophthora blights in both pepper seedlings and pepper fruits. Treatment with CA resulted in collapsed and fragmented hyphae, accompanying the increase in MDA (malondialdehyde) content and the decrease in intercellular glycerol content in hyphae. CA also inhibited the growth of wild type yeast. The yeast mutant ΔYvc1 with a deletion of Yvc1 (a Ca2+ transporter) showed decreased sensitivity to CA. The transformation of PcNCX1, a sodium/calcium exchanger from P. capsici, into ΔYvc1 restored its sensitivity to CA. The transformant carrying PcNCX1 also showed restored Ca2+ efflux upon CA treatment. RNA-seq analysis showed that CA treatments resulted in the down-regulation of a set of genes encoding for calcium-related proteins. Collectively, our study demonstrates that the antifungal activity of CA against P. capsici may be associated with PcNCX1-mediated Ca2+ efflux. Our results provide crucial insights into the antimicrobial action of CA. [ABSTRACT FROM AUTHOR]

This paper investigates the impact of high-speed railways (HSR) on urban innovation levels by improving intercity accessibility. We employ prefecture city-level data within three megacity regions in China from 2009 to 2018. Using the number of invention patents granted as a proxy for the innovation level of a city, we find that HSR facilities significantly improve urban innovation levels through better regional intercity accessibility and that there is a diminishing effect as commuting time increases. The impact mechanisms of innovation improvement can be explained by an ever-increasing potential of interaction activities among talents and technology investment opportunities among cities. We contribute to the literature by highlighting the spatial attenuation impact of HSR on urban innovation levels as well as the underlying mechanisms. Particularly, talent interaction exerts a larger effect on urban innovation levels than technology investment opportunities. Moreover, we unravel the heterogeneous effects that more innovative cities and cities with double first-class universities gain more from the improved intercity accessibility brought by HSR. This research has policy implications that promoting HSR facilities improves innovation levels of cities with different resources. [ABSTRACT FROM AUTHOR]

High-temperature superconductor (HTS) bulks have shown very promising potential for industrial applications due to the ability to trap much higher magnetic fields compared to traditional permanent magnets. In rotating electrical machines, the magnetic field is a combination of alternating and rotating fields. On the contrary, all studies on electromagnetic characterization of HTS presented in the literature so far have only focused on alternating AC magnetic fields and alternating AC loss due to the unavailability of robust experimental techniques and analytical models. This paper presents a numerical investigation on the characterization of HTS bulks subjected to rotating magnetic fields showing AC loss, current density distribution in three-dimensional axes, and trapped field analysis. A three-dimensional numerical model has been developed using H-formulation based on finite element analysis. An HTS cubic sample is magnetized and demagnetized with two-dimensional magnetic flux density vectors rotating in circular orientation around the XOY, XOZ, and YOZ planes. [ABSTRACT FROM AUTHOR]

The past decade has seen a surge in the use of city branding, which is used to attract specific target groups of investors, high-tech green firms and talented workforce and reflects a desired shift from old, polluting manufacturing industries to new, clean service industries. Previous studies in the Chinese mega-city regions Pearl River Delta, Yangtze River Delta and Jing-Jin-Ji (region around Beijing and Tianjin) have shown that branding practices of primarily service and innovation oriented cities are largely in line with existing industrial profiles while those which are predominantly manufacturing oriented wish to present themselves as more service and innovation driven. In this contribution, city branding practices are studied in China's three Northeastern provinces Heilongjiang, Jilin and Liaoning which face structural decline because of the presence of many outdated resource-based and heavy industries. The gap between existing profile and branding choices appears not systematic as in China's leading economic regions. Northeastern cities focus more on combining primary, secondary and tertiary industrial patterns than on displacing manufacturing with services. The tertiary sector in these provinces is more administrative and public sector oriented and generates lower value added; it is therefore not significantly more attractive than the primary and secondary ones. [ABSTRACT FROM AUTHOR]

Plants are always exposed to the environment, polluted by multiple trace elements. Hydrogen sulfide (H2S), an endogenous gaseous transmitter in plant cells, can help plant combat single elements with excess concentration. Until now, little has been known about the regulatory role of H2S in response to combined stress of multiple elements. Here we found that combined exposure of mercury (Hg) and selenium (Se) triggered endogenous H2S signal in the roots of Brasscia rapa. However, neither Hg nor Se alone worked on it. In roots upon Hg + Se exposure, the defensive role of endogenous H2S was associated to the decrease in reactive oxygen species (ROS) level, followed by alleviating cell death and recovering root growth. Such findings extend our knowledge of plant H2S in response to multiple stress conditions. [ABSTRACT FROM AUTHOR]

The rapid and direct structural characterization of lipids proves to be critical for studying the functional roles of lipids in many biological processes. Among numerous analytical techniques, ambient ionization mass spectrometry (AIMS) allows for a direct molecular characterization of lipids from various complex biological samples with no/minimal sample pretreatment. Over the recent years, researchers have expanded the applications of the AIMS techniques to lipid structural elucidation via a combination with a series of derivatization strategies (e.g., the Paternò–Büchi (PB) reaction, ozone-induced dissociation (OzID), and epoxidation reaction), including carbon–carbon double bond (C=C) locations and sn-positions isomers. Herein, this review summarizes the reaction mechanisms of various derivatization strategies for C=C bond analysis, typical instrumental setup, and applications of AIMS in the structural elucidation of lipids from various biological samples (e.g., tissues, cells, and biofluids). In addition, future directions of AIMS for lipid structural elucidation are discussed. [ABSTRACT FROM AUTHOR]

There has been an increasing number of studies on the interaction between active substances and probiotics to improve disease. Both krill oil (KO) and probiotics have the effect of improving atherosclerotic cardiovascular disease, but the combined effect has not been explored. Therefore, the purpose of this study was to explore the improvement effect of KO combined with probiotics on atherosclerosis. The atherosclerotic plaque area of ApoE−/− mice was detected after the intervention of KO, Bifidobacterium animalis subsp. lactis F1-7 (Bif. animalis F1-7), and KO combined with Bif. animalis F1-7. The results showed that Bif. animalis F1-7, KO, and KO combined with Bif. animalis F1-7 could significantly reduce the area of atherosclerotic plaque and improve the levels of serum lipids and inflammatory factors. They could regulate the farnesoid X receptor (FXR)/cholesterol 7-alpha hydroxylase (CYP7A1) pathway to reduce lipid accumulation. The intervention groups could also improve the inflammatory response by downregulating the Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88) pathway. The anti-inflammatory effect of the interaction group was significantly better than that of KO. It proved that Bif. animalis F1-7 might play a synergistic effect in the improvement of inflammation by KO to the alleviation of atherosclerosis. [ABSTRACT FROM AUTHOR]

With the challenge to reach targets of carbon emission reduction at the regional level, it is necessary to analyze the regional differences and influencing factors on China's carbon emission efficiency. Based on statistics from 2005 to 2015, carbon emission efficiency and the differences in 30 provinces of China were rated by the Modified Undesirable Epsilon-based measure (EBM) Data Envelopment Analysis (DEA) Model. Additionally, we further analyzed the influencing factors of carbon emission efficiency's differences in the Tobit model. We found that the overall carbon emission efficiency was relatively low in China. The level of carbon emission efficiency is the highest in the East region, followed by the Central and West regions. As for the influencing factors, industrial structure, external development, and science and technology level had a significant positive relationship with carbon emission efficiency, whereas government intervention and energy intensity demonstrated a negative correlation with carbon emission efficiency. The contributions of this paper include two aspects. First, we used the Modified Undesirable EBM DEA Model, which is more accurate than traditional methods. Secondly, based on the data's unit root testing and cointegration, the paper verified the influencing factors of carbon emission efficiency by the Tobit model, which avoids the spurious regression. Based on the results, we also provide several policy implications for policymakers to improve carbon emission efficiency in different regions. [ABSTRACT FROM AUTHOR]

Place branding is seriously studied in various academic disciplines. Its impact on strategic development plays a vital role in processes of sustainable urban transformation. Place branding originated in tourism management and over time its research focus spilled over and evolved in environmental science, urban studies and public administration. Scholars and policy makers are currently faced with a myriad of concepts in place branding research, which show considerable overlap but should be carefully distinguished from each other. To increase our understanding of this body of research, this article observes a strong pattern of the concepts in use, in their references to location types (i.e., urban, city, destination, place) and broadcasting activities (branding, marketing, promotion). It builds on this observation by collecting studies from the Scopus database by location type and broadcasting activities (LT–BA) reference pairs and systematically analysing and reviewing these from 1980 to 2018. A total of 2665 articles and reviews were identified and analysed based on (1) occurrences per reference pair, (2) co-occurrences per reference pair, and (3) co-occurrences for each reference pair with other concepts. On that basis, the origin and evolution of the research field including multiple reference pairs in use is explored and described. [ABSTRACT FROM AUTHOR]

Hexavalent chromium [Cr(VI)] is a known human lung carcinogen with widespread exposure in environmental and occupational settings. Despite well-known cancer risks, the molecular mechanisms of Cr(VI)-induced carcinogenesis are not well understood, but a major driver of Cr(VI) carcinogenesis is chromosome instability. Previously, we reported Cr(VI) induced numerical chromosome instability, premature centriole disengagement, centrosome amplification, premature centromere division, and spindle assembly checkpoint bypass. A key regulator of these events is securin, which acts by regulating the cleavage ability of separase. Thus, in this study we investigated securin disruption by Cr(VI) exposure. We exposed human lung cells to a particulate Cr(VI) compound, zinc chromate, for acute (24 h) and prolonged (120 h) time points. We found prolonged Cr(VI) exposure caused marked decrease in securin levels and function. After prolonged exposure at the highest concentration, securin protein levels were decreased to 15.3% of control cells, while securin mRNA quantification was 7.9% relative to control cells. Additionally, loss of securin function led to increased separase activity manifested as enhanced cleavage of separase substrates; separase, kendrin, and SCC1. These data show securin is targeted by prolonged Cr(VI) exposure in human lung cells. Thus, a new mechanistic model for Cr(VI)-induced carcinogenesis emerges with centrosome and centromere disruption as key components of numerical chromosome instability, a key driver in Cr(VI) carcinogenesis.