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With the advantages of a high specificity, a long half-life, and a high safety, the use of antibody biologic drugs, including camrelizumab, has been rapidly increasing in clinical practice. Camrelizumab, an immune checkpoint inhibitor and humanized monoclonal antibody, is used to treat several advanced solid cancers. Measuring its concentration supports personalized dosage adjustments, influences treatment decisions for patients, strengthens the control of disease activity through therapeutic drug monitoring, and helps evaluate and prevent drug interactions in combination therapy. Because antibodies are present in complex biological matrices, quantifying monoclonal antibody drugs is challenging, and must rely on precise, selective, and reliable analytical methods. In this study, a quadrupole time-of-flight mass spectrometry TripleTOF 6600+ (AB SCIEX, Framingham, MA, USA) system equipped with a Turbo V ion source was used for the qualitative analysis of monoclonal antibodies using the data-dependent acquisition (IDA) MS/MS mode, followed by quantitative analysis using a targeted MRMHR workflow. This method showed a good linear relationship within the range of 4–160 μg/mL, with a correlation coefficient of R2 ≥ 0.996. It demonstrated an acceptable accuracy (88.95–101.18%) and precision (≤15%). Furthermore, the lower limit of quantification was found to be 4 μg/mL, with the lowest detection limit of 0.3217 μg/mL, indicating that this method is rapid, accurate, and reliable for the quantitative analysis of camrelizumab in human serum. [ABSTRACT FROM AUTHOR]

Simple Summary: This study aimed to estimate the phenotypic and genetic parameters of milk production rate (MPR) traits in Holstein cattle. The MPR, a metric for evaluating a cow's milk secretion per hour, was calculated using milk yield and milking interval data from 4760 cows. This study defined four milk yield and six MPR traits, and used the MIXED procedure to assess the effects of parity, season, and lactation stage on these traits. Significant effects of these non-genetic factors were found for both milk yield and MPR traits. Heritability estimates for milk yield and MPR traits were high, ranging from 0.25 to 0.39, while the stability of MPR had low heritability (0.04 to 0.05). This study concluded that MPR is a valuable trait for dairy breeding, providing new insights for herd management and genetic selection to improve dairy cattle production performance. Milk production rate (MPR) refers to the rate of milk secretion per hour (kg/h), calculated from the harvested milk yield and milking interval, and it is considered an appropriate measure to evaluate the production potential of cows. The objective of this study was to estimate the phenotypic and genetic parameters of milk production rate traits. In this study, the milking records of 4760 Holstein cows were collected, and four milk yield traits and six milk production rate traits were defined. The MIXED procedure was used to detect the impacts of non-genetic effects on milk yield and milk production rate traits, including parity, measured season and lactation stage. Variance and covariance components for milk yield and milk production rate traits were estimated using a univariate linear repeatability model. Parity, measurement season and lactation stage had significant effects (p < 0.01) on milk yield, milk production rate and its stability. Milk yield and milk production traits had high heritability, and ranged from 0.25 to 0.39. The stability of milk production rate had low heritability (0.04~0.05). Milk production rate is beneficial for the devolving novel trait in dairy breeding and provides new insights for herd management and genetic selection of production performance of dairy cattle. [ABSTRACT FROM AUTHOR]

The shared energy storage device acts as an energy hub between multiple microgrids to better play the complementary characteristics of the microgrid power cycle. In this paper, the cooperative operation process of shared energy storage participating in multiple island microgrid systems is researched, and the two-stage research on multi-microgrid operation mode and shared energy storage optimization service cost is focused on. In the first stage, the output of each subject is determined with the goal of profit optimization and optimal energy storage capacity, and the modified grey wolf algorithm is used to solve the problem. In the second stage, the income distribution problem is transformed into a negotiation bargaining process. The island microgrid and the shared energy storage are the two sides of the game. Combined with the non-cooperative game theory, the alternating direction multiplier method is used to reduce the shared energy storage service cost. The simulation results show that shared energy storage can optimize the allocation of multi-party resources by flexibly adjusting the control mode, improving the efficiency of resource utilization while improving the consumption of renewable energy, meeting the power demand of all parties, and realizing the sharing of energy storage resources. Simulation results show that compared with the traditional PSO algorithm, the iterative times of the GWO algorithm proposed in this paper are reduced by 35.62%, and the calculation time is shortened by 34.34%. Compared with the common GWO algorithm, the number of iterations is reduced by 18.97%, and the calculation time is shortened by 22.31%. [ABSTRACT FROM AUTHOR]

Due to the large-scale integration of renewable energy and the rapid growth of peak load demand, it is necessary to comprehensively consider the construction of various resources to increase the acceptance capacity of renewable energy and meet power balance conditions. However, traditional grid planning methods can only plan transmission lines, often resulting in low utilization rates of newly constructed lines. Additionally, static planning methods can only address single-target scenarios and cannot cope with dynamic growth in load and renewable energy. To address these issues, this paper proposes a multi-stage collaborative planning method for transmission networks and energy storage. This method considers the non-line substitution effect of energy storage resources and their characterization methods. It establishes the coupling relationship between resources across different planning stages to achieve coordinated multi-stage planning for transmission networks and energy storage. Based on the IEEE-24 node system and a case study in a northern province of China, the results show that the proposed method reduces investment costs by approximately 30% compared to static planning methods and by about 7.79% compared to conventional grid planning methods. Furthermore, this method can accommodate more renewable energy. [ABSTRACT FROM AUTHOR]

Field determination and ecological risk assessment of dissolved lead (Pb) were performed at two Yellow Sea sites in China using a continuous automated electrochemical system (CAEDS). This CAEDS instrument includes an automatic triple filter sampler and an electrochemical detection water quality analyzer, which might be operated automatically four times daily. The dissolved Pb concentrations varied from 0.29 to 1.57 μg/L in the South Yellow Sea over 16 days and from 0.32 to 2.28 μg/L in the North Yellow Sea over 13 days. During the typhoon and algal bloom periods, the Pb concentration was as high as ten times greater than usual. According to the calculation of contamination factors (Cf) and subsequent analysis, seawater quality was classified as Grade II. Through species sensitivity distribution (SSD) method experiments and ecological risk analysis, an average risk quotient (RQ) below 1 for both areas was obtained, indicating a low-to-moderate ecological risk. This system will be helpful for Pb monitoring and assessment in seawater and contribute to the biogeochemical cycling study of Pb. [ABSTRACT FROM AUTHOR]

Increasing array gains is one of the keys to improving underwater targets' detection capabilities. This paper presents a high-gain approach for a vector hydrophone vertical array (VHVA) that combines white noise gain constraint (WNGC) with vector joint processing to preserve strong robustness and provide noticeable gains. Firstly, this approach treats the VHVA as four independent sub-arrays and achieves sub-array supergains by decorrelating noise using WNGC. The beam outputs of the four sub-arrays are then equated to a single-vector hydrophone, the combination gain of which is obtained by leveraging the strong signal correlation and the weak noise correlation between the sound pressure and the particle velocity. Lastly, the sub-array supergain and combination gain are superposed to provide the spatial gain of the VHVA. It is also summarized that low-frequency signals, coherent noise, accurate elevation-angle estimation, and stable phase differences are required for the VHVA to achieve supergain. The simulation and sea trial confirm that this approach can effectively boost the array gain. The maximum spatial gain in the experiment was increased by 9 dB at a range twice the sea's depth while operating at a low frequency. This method shows enormous potential for improving the performance of deep-sea target detection. [ABSTRACT FROM AUTHOR]

Bacillus velezensis is a promising candidate for biocontrol applications. A common second messenger molecule, bis-(3,5)-cyclic-dimeric-guanosine monophosphate (c-di-GMP), has the ability to regulate a range of physiological functions that impact the effectiveness of biocontrol. However, the status of the c-di-GMP signaling pathway in biocontrol strain LQ-3 remains unknown. Strain LQ-3, which was isolated from wheat rhizosphere soil, has shown effective control of wheat sharp eyespot and has been identified as B. velezensis through whole-genome sequencing analyses. In this study, we investigated the intracellular c-di-GMP signaling pathway of LQ-3 and further performed a comparative genomic analysis of LQ-3 and 29 other B. velezensis strains. The results revealed the presence of four proteins containing the GGDEF domain, which is the conserved domain for c-di-GMP synthesis enzymes. Additionally, two proteins were identified with the EAL domain, which represents the conserved domain for c-di-GMP degradation enzymes. Furthermore, one protein was found to possess a PilZ domain, indicative of the conserved domain for c-di-GMP receptors in LQ-3. These proteins are called DgcK, DgcP, YybT, YdaK, PdeH, YkuI, and DgrA, respectively; they are distributed in a similar manner across the strains and belong to the signal transduction family. We selected five genes from the aforementioned seven genes for further study, excluding YybT and DgrA. They all play a role in regulating the motility, biofilm formation, and colonization of LQ-3. This study reveals the c-di-GMP signaling pathway associated with biocontrol features in B. velezensis LQ-3, providing guidance for the prevention and control of wheat sharp eyespot by LQ-3. [ABSTRACT FROM AUTHOR]

Currently, the clinical cure rate for primary liver cancer remains low. Effective screening and early diagnosis of hepatocellular carcinoma (HCC) remain clinical challenges. Exosomes are intimately associated with tumor development and their contents have the potential to serve as highly sensitive tumor-specific markers. A comprehensive untargeted metabolomics study was conducted using exosome samples extracted from the serum of 48 subjects (36 HCC patients and 12 healthy controls) via a commercial kit. An ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) strategy was used to identify the metabolic compounds. A total of 18 differential metabolites were identified using the non-targeted metabolomics approach of UPLC-QTOF-MS/MS. Pathway analysis revealed significant alterations in the arachidonic acid metabolism, linoleic acid metabolism, and unsaturated fatty acid metabolism pathways. ROC analysis indicated that three metabolites with AUC values exceeding 0.900 were selected as potential biomarkers: caprylic acid and linoleic acid were upregulated in the HCC group, whereas pentadecanoic acid was downregulated. Linoleic acid, caprylic acid, and pentadecanoic acid are potential biomarkers for diagnosing HCC. The significant alterations in these three metabolic pathways offer new insights into the mechanisms underlying HCC formation and progression. [ABSTRACT FROM AUTHOR]

Ink-jet-printed silk, a premium textile material, was achieved by utilizing a bio-based gardenia blue dye. However, the sharpness of the printing pattern is difficult to control due to the limited water-retention capacity of silk. To address this issue, three polysaccharide derivatives, namely, sodium alginate (SA), low-viscosity hydroxypropyl methyl cellulose (HPMC-I), and high-viscosity hydroxypropyl methyl cellulose (HPMC-II), were employed as thickeners to modify the silk by the dipping–padding method. Firstly, the preparation of the gardenia blue ink and the rheology assessment of the thickener solution were conducted. Furthermore, the impacts of different thickeners on the micro-morphology, element composition, and hydrophilicity of the silk, along with the wetting behavior of the ink on the silk, were analyzed comparatively in order to identify an appropriate thickener for preserving pattern outlines. Lastly, the color features, color fastness, and wearing characteristics of the printed silk were discussed to evaluate the overall printing quality. Research results showed that the optimized ink formulation, comprising 12% gardenia blue, 21% alcohols, and 5.5% surfactant, met the requirements for ink-jet printing (with a viscosity of 4.48 mPa·s, a surface tension of 34.12 mN/m, and a particle size of 153 nm). The HPMC-II solution exhibited prominent shear-thinning behavior, high elasticity, and thixotropy, facilitating the achievement of an even modification effect. The treatment of the silk with HPMC-II resulted in the most notable decrease in hydrophilicity. This can be attributed to the presence of filled gaps and a dense film on the fibers' surface after the HPMC-II treatment, as observed by scanning electron microscopy. Additionally, X-ray photoelectron spectroscopy analysis confirmed that the HPMC-II treatment introduced the highest content of hydrophobic groups on the fiber surface. The reduced hydrophilicity inhibited the excessive diffusion and penetration of gardenia blue ink, contributing to a distinct printing image and enhanced apparent color depth. Moreover, the printed silk demonstrated qualified color fastness to rubbing and soaping (exceeding grade four), a soft handle feeling, an ignorable strength loss (below 5%), and a favorable air/moisture penetrability. In general, the surface modification with the HPMC-II treatment has been proven as an effective strategy for upgrading the image quality of bio-based dye-printed silk. [ABSTRACT FROM AUTHOR]

Aiming at the shortcomings of traditional fault identification methods in fault information acquisition, In the scenario of hybrid HVDC transmission system, a new fault identification method is proposed by using wavelet packet energy spectrum and convolutional neural network (CNN), which effectively solves the problem of complex fault feature extraction of hybrid HVDC transmission system. This method effectively improves the accuracy of fault identification. Firstly, tThe frequency-domain characteristics of the fault transient signal are extracted by wavelet packet transform, and the feature differences are reflected in the form of energy spectrum. Secondly, according to the extracted energy feature information, the order of fault line and fault type is identified by CNN. Finally, through example verification and algorithm comparison, it is concluded that, the mentioned model has a strong ability to identify faults, and has strong anti-noise interference and tolerance to transition resistance. [ABSTRACT FROM AUTHOR]

Weaknesses generated in asphalt pavement structures have a serious impact on the service life of pavements. In order to improve such situations and achieve the goal of enhancing the durability of the pavement structure, this study assesses the performance of heavy-duty asphalt and high-viscosity asphalt, using four high-performance asphalt mixtures: heavy-duty AC-20, high-viscosity AC-20, heavy-duty SMA-13, and heavy-duty SMA-10. Three composite pavement structures were designed: 3 cm SMA-10 + 3 cm SMA-10, 4 cm SMA-13 + 4 cm SMA-10, and 6 cm SMA-13 + 4 cm AC-20. Interlayer performance analysis was conducted on single-layer and composite structures through oblique shear tests; dynamic modulus, fatigue life, and antirutting performance tests on asphalt pavement structural layers were designed and conducted, and the durability performance of high-performance asphalt pavement structural layers was evaluated. The experimental results show that the shear strength of heavy-duty AC is higher than that of heavy-duty SMA, the 4 + 4 combination structure has the best shear strength, the 6 + 4 combination structure has the best structural performance and fatigue resistance, and the 3 + 3 combination structure has the best high-temperature antirutting performance. The comprehensive performance of the 4 + 4 structure is the best among the three combined structures, followed by that of the 6 + 4 structure, and the performance of the 3 + 3 structure is the worst. In addition, this study used bonding energy as an evaluation index and verified the applicability of the bonding energy evaluation index by studying four types of single-layer pavement structures and three types of composite pavement structures. [ABSTRACT FROM AUTHOR]

In adapting to the double-high development trend of high-voltage direct current (HVDC) receiving-end power systems and solving optimization problems in emergency frequency control (EFC) supporting virtual power plants (VPPs) in large-scale power systems, a parameter estimation method for a VPP frequency response model based on a successive linear programming (SLP) method is proposed. First, a "centralized/decentralized" hierarchical control architecture for VPP participation in EFC is designed. Second, the frequency response characteristics of multiple flexible resources are scientifically analyzed, and the system frequency response (SFR) model and equivalent model of VPP are constructed. Subsequently, parameter estimation of the VPP frequency response model is carried out based on the SLP method, aiming to balance the accuracy and computational efficiency of the model. Finally, the effectiveness of the proposed methodology is verified by using PSD-BPA to simulate and test the three-zone HVDC recipient area grid. [ABSTRACT FROM AUTHOR]

Enterococcus faecalis is a prevalent opportunistic pathogen associated with chicken embryonic and neonatal chick mortality, posing a significant challenge in poultry farming. In the current study, E. faecalis strain EF6, isolated from a recent hatchery outbreak, served as the host bacterium for the isolation of a novel phage EFP6, capable of lysing E. faecalis. Transmission electron microscopy revealed a hexagonal head and a short tail, classifying EFP6 as a member of the Autographiviridae family. EFP6 showed sensitivity to ultraviolet radiation and resistance to chloroform. The lytic cycle duration of EFP6 was determined to be 50 min, highlighting its efficacy in host eradication. With an optimal multiplicity of infection of 0.001, EFP6 exhibited a narrow lysis spectrum and strong specificity towards host strains. Additionally, EFP6 demonstrated optimal growth conditions at 40 °C and pH 8.0. Whole genome sequencing unveiled a genome length of 18,147 bp, characterized by a GC concentration of 33.21% and comprising 25 open reading frames. Comparative genomic assessment underscored its collinearity with related phages, notably devoid of lysogenic genes, thus ensuring genetic stability. This in-depth characterization forms the basis for understanding the biological attributes of EFP6 and its potential utilization in phage therapy, offering promising prospects for mitigating E. faecalis-associated poultry infections. [ABSTRACT FROM AUTHOR]

In order to solve the demand for efficient and stable low-voltage, high-power energy transmission capacity of electric vehicle (EV) fast charging, an ISOP-IPT system based on inductor-capacitor-capacitor series (LCC-S) compensation network is proposed. Firstly, the influence of compensation parameter inconsistency on the system is analyzed. On this basis, considering the resistance of coupler coils, the overall transmission efficiency of the system is analyzed. It is found that the voltage imbalance of the system will affect the working state of inverters, and then affect the stability of the system. The system transmission efficiency increases with the increase in mutual inductance. Moreover, the voltage imbalance caused by the inconsistency of compensation parameters and mutual inductance will reduce the transmission efficiency of the system. Finally, it is concluded that in the parameter design of the ISOP-IPT system, mutual inductance should be improved on the basis of ensuring the input voltage equalization of each channel so as to improve the transmission efficiency and working stability of the system. The experimental platform of a two-channel ISOP-IPT system is built and the maximum efficiency is 94.03%, which verifies the correctness of theoretical analysis. [ABSTRACT FROM AUTHOR]

Simple Summary: The current study aimed to investigate the virulence and resistance genes associated with Klebsiella pneumoniae isolated from the farms of four animal species in selected areas of Xinjiang, China, encompassing cows, sheep, chickens, and pigs. The sensitivity to antibiotics was evaluated. Genetic diversity was assessed using wzi allele typing and multilocus sequence typing. The findings shed light on the antibiotic resistance patterns and genetic diversity among K. pneumoniae isolates originating from different animal sources, highlighting the potential for inter-species transmission of this bacterium. Klebsiella pneumoniae (K. pneumoniae) is recognized as a zoonotic pathogen with an increasing threat to livestock and poultry. However, research on K. pneumoniae of animal origin remains limited. To address the gap, a comprehensive investigation was carried out by collecting a total of 311 samples from the farms of four animal species (dairy cow, chicken, sheep, and pig) in selected areas of Xinjiang, China. Isolates were identified by khe gene amplification and 16S rRNA gene sequencing. Genotyping of K. pneumonia isolates was performed using wzi typing and multilocus sequence typing (MLST). PCR was employed to identify virulence and resistance genes. An antibiotic susceptibility test was conducted using the Kirby–Bauer method. The findings revealed an isolation of 62 K. pneumoniae strains, with an average isolation rate of 19.94%, with the highest proportion originating from cattle sources (33.33%). Over 85.00% of these isolates harbored six virulence genes (wabG, uge, fimH, markD, entB, and ureA); while more than 75.00% of isolates possessed four resistance genes (blaTEM, blaSHV, oqxA, and gyrA). All isolates exhibited complete resistance to ampicillin and demonstrated substantial resistance to sulfisoxazole, amoxicillin/clavulanic acid, and enrofloxacin, with an antibiotic resistance rate of more than 50%. Furthermore, 48.39% (30/62) of isolates were classified as multidrug-resistant (MDR) strains, with a significantly higher isolation rate observed in the swine farms (66.67%) compared to other farms. Genetic characterization revealed the classification of the 62 isolates into 30 distinct wzi allele types or 35 different sequence types (STs). Notably, we identified K. pneumoniae strains of dairy and swine origin belonging to the same ST42 and wzi33-KL64 types, as well as strains of dairy and chicken origin belonging to the same wzi31-KL31-K31 type. These findings emphasize the widespread occurrence of drug-resistant K. pneumoniae across diverse animal sources in Xinjiang, underscoring the high prevalence of multidrug resistance. Additionally, our results suggest the potential for animal-to-animal transmission of K. pneumoniae and there was a correlation between virulence genes and antibiotic resistance genes. Moreover, the current study provides valuable data on the prevalence, antibiotic resistance, and genetic diversity of K. pneumoniae originating from diverse animal sources in Xinjiang, China. [ABSTRACT FROM AUTHOR]

Peanut is an important oilseed and a widely cultivated crop worldwide. Knowledge of the phylogenetic relationships and information on the chloroplast genomes of wild and cultivated peanuts is crucial for the evolution of peanuts. In this study, we sequenced and assembled 14 complete chloroplast genomes of Arachis. The total lengths varied from 156,287 bp to 156, 402 bp, and the average guanine–cytosine content was 36.4% in 14 Arachis species. A total of 85 simple sequence repeats (SSRs) loci were detected, including 3 dinucleotide and 82 polynucleotide SSRs. Based on 110 complete chloroplast genomes of Arachis, a phylogenetic tree was constructed, which was divided into two groups (I and II). A total of 79 different genes were identified, of which six double-copy genes (ndhB, rpl2, rpl23, rps7, ycf1, and ycf2) and one triple-copy gene (rps12) are present in all 14 Arachis species, implying that these genes may be critical for photosynthesis. The dN/dS ratios for four genes (rps18, accD, clpP, ycf1) were larger than 1, indicating that these genes are subject to positive selection. These results not only provided rich genetic resources for molecular breeding but also candidate genes for further functional gene research. [ABSTRACT FROM AUTHOR]

Soil salinity imposes osmotic, ionic, and oxidative stresses on plants, resulting in growth inhibition, developmental changes, metabolic adaptations, and ion sequestration or exclusion. Identifying salinity-tolerant resources and understanding physiological and molecular mechanisms of salinity tolerance could lay a foundation for the improvement of salinity tolerance in rice. In this study, a series of salinity-tolerance-related morphological and physiological traits were investigated in 46 rice genotypes, including Sea Rice 86, to reveal the main strategies of rice in responding to salinity stress at the seedling stage. No genotypes showed the same tolerance level as the two landraces Pokkali and Nona Bokra, which remain the donors for improving the salinity tolerance of rice. However, due to undesirable agronomic traits of these donors, alternative cultivars such as JC118S and R1 are recommended as novel source of salinity tolerance. Correlation and principal component analyses revealed that the salinity tolerance of rice seedlings is not only controlled by growth vigor but also regulated by ion transport pathways such as long-distance Na+ transport, root Na+ sequestration, and root K+ retention. Therefore, such key traits should be targeted in future breeding programs as the strategy of obtaining better Na+ exclusion is still the bottleneck for improving salinity tolerance in rice. [ABSTRACT FROM AUTHOR]

Gardenia is both a food and medicine plant. It is widely used for cardiovascular protection, and its main bioactive ingredient is crocetin. This study aims to observe the therapeutic effects of crocetin on chronic heart failure in rats induced by various etiologies. It further compares the efficacy differences between preventative and treatment administration, varying dosages, and treatment durations, to provide improved guidance for medication in heart failure rats and determine which categories of chronic heart failure rats might benefit most from crocetin. Chronic heart failure models induced by abdominal aorta constriction, renal hypertension, and coronary artery ligation were constructed. By examining cardiac function, blood biochemistry, and histopathology, the study assessed the preventive and therapeutic effects of crocetin on load-induced and myocardial ischemia-induced heart failure. The results showed that in all three models, both treatment and preventative administration of crocetin significantly improved chronic heart failure in rats, especially in preventative administration. The results indicate crocetin may be beneficial for improving symptoms and functional capacity in rats with heart failure. Furthermore, long-term administration was more effective than short-term administration across all three rat models, with therapeutic onset observed over 6 weeks. [ABSTRACT FROM AUTHOR]

Marine mangrove vegetation has been traditionally employed in folk medicine to address various ailments. Notably, Rhizophora apiculata Blume has exhibited noteworthy properties, demonstrating efficacy against cancer, viruses, and bacteria. The enzyme fatty acid synthase (FAS) plays a pivotal role in de novo fatty acid synthesis, making it a promising target for combating colon cancer. Our study focused on evaluating the FAS inhibitory effects of both the crude extract and three isolated compounds from R. apiculata. The n-butanol fraction of R. apiculata extract (BFR) demonstrated a significant inhibition of FAS, with an IC50 value of 93.0 µg/mL. For inhibition via lyoniresinol-3α-O-β-rhamnopyranoside (LR), the corresponding IC50 value was 20.1 µg/mL (35.5 µM). LR competitively inhibited the FAS reaction with acetyl-CoA, noncompetitively with malonyl-CoA, and in a mixed manner with NADPH. Our results also suggest that both BFR and LR reversibly bind to the KR domain of FAS, hindering the reduction of saturated acyl groups in fatty acid synthesis. Furthermore, BFR and LR displayed time-dependent inhibition for FAS, with kobs values of 0.0045 min−1 and 0.026 min−1, respectively. LR also exhibited time-dependent inhibition on the KR domain, with a kobs value of 0.019 min−1. In human colon cancer cells, LR demonstrated the ability to reduce viability and inhibit intracellular FAS activity. Notably, the effects of LR on human colon cancer cells could be reversed with the end product of FAS-catalyzed chemical reactions, affirming the specificity of LR on FAS. These findings underscore the potential of BFR and LR as potent FAS inhibitors, presenting novel avenues for the treatment of human colon cancer. [ABSTRACT FROM AUTHOR]

The Euodia genus comprises numerous untapped medicinal plants that warrant thorough evaluation for their potential as valuable natural sources of herbal medicine or food flavorings. In this study, untargeted metabolomics and in vitro functional methods were employed to analyze fruit extracts from 11 significant species of the Euodia genus. An investigation of the distribution of metabolites (quinolone and indole quinazoline alkaloids) in these species indicated that E. rutaecarpa (Euodia rutaecarpa) was the most widely distributed species, followed by E. compacta (Euodia compacta), E. glabrifolia (Euodia glabrifolia), E. austrosinensis (Euodia austrosinensis), and E. fargesii (Euodia fargesii). There have been reports on the close correlation between indole quinazoline alkaloids and their anti-tumor activity, especially in E. rutaecarpa fruits which exhibit effectiveness against various types of cancer, such as SGC-7901, Hela, A549, and other cancer cell lines. Additionally, the E. rutaecarpa plant contains indole quinazoline alkaloids, which possess remarkable antibacterial properties. Our results offer novel insights into the utilization of Euodia resources in the pharmaceutical industry. [ABSTRACT FROM AUTHOR]

Ampicillin (AMP) and cefazolin (CZO) are commonly used β-lactam antibiotics which are extensively globally produced. Additionally, AMP and CZO are known to have relatively high ecotoxicity. Notably, the mix of AMP and CZO creates a synergistic effect that is more harmful to the environment, and how exposure to AMP-CZO can induce synergism in algae remains virtually unknown. To yield comprehensive mechanistic insights into chemical toxicity, including dose–response relationships and variations in species sensitivity, the integration of multiple endpoints with de novo transcriptomics analyses were used in this study. We employed Selenastrum capricornutum to investigate its toxicological responses to AMP and CZO at various biological levels, with the aim of elucidating the underlying mechanisms. Our assessment of multiple endpoints revealed a significant growth inhibition in response to AMP at the relevant concentrations. This inhibition was associated with increased levels of reactive oxygen species (ROS) and perturbations in nitrogen metabolism, carbohydrate metabolism, and energy metabolism. Growth inhibition in the presence of CZO and the AMP-CZO combination was linked to reduced viability levels, elevated ROS production, decreased total soluble protein content, inhibited photosynthesis, and disruptions in the key signaling pathways related to starch and sucrose metabolism, ribosome function, amino acid biosynthesis, and the production of secondary metabolites. It was concluded from the physiological level that the synergistic effect of Chlorophyll a (Chla) and Superoxide dismutase (SOD) activity strengthened the growth inhibition of S. capricornutum in the AMP-CZO synergistic group. According to the results of transcriptomic analysis, the simultaneous down-regulation of LHCA4, LHCA1, LHCA5, and sodA destroyed the functions of the photosynthetic system and the antioxidant system, respectively. Such information is invaluable for environmental risk assessments. The results provided critical knowledge for a better understanding of the potential ecological impacts of these antibiotics on non-target organisms. [ABSTRACT FROM AUTHOR]

A portable electrochemical system based on rGO/AuNPs/Nafion was constructed for selenium (IV) field determination in coastal seawater of the North Yellow Sea, East China Sea and South China Sea. This system demonstrated a low determination limit (0.03 μg/L), a wide linear range of concentration (0.1–30 μg/L), exceptional reproducibility and repeatability, and excellent selectivity. Each individual, entire test could be accomplished within 8 min. In Yantai Sishili Bay, the Se (IV) concentrations ranged from 0.16–3.59 μg/L (average 1.43 μg/L). In Shengshan Island, the Se (IV) concentrations ranged from 0.20–5.18 μg/L (average 2.06 μg/L). In Zhelin Bay, the Se (IV) concentrations ranged from 0.87–4.87 μg/L (average 3.13 μg/L). The average concentrations of Se (IV) in surface seawater showcased an increasing trend from the north to the south of China, suggesting Zhelin Bay > Shengshan Island > Sishili Bay. The system develops a new, competitive method for water quality assessment, and contributes to the accurate study of the biogeochemical cycles of selenium. [ABSTRACT FROM AUTHOR]

Simple Summary: Modern agricultural methods have led to the introduction of normally highland Tibetan pigs to lowland areas because of their high-quality meat. Similar to humans, indigenous Tibetan pigs residing in high-altitude regions may develop high-altitude de-acclimatization syndrome when exposed to low-altitude environments. Thus, it is important to better understand the low-altitude adaptation mechanisms of Tibetan pigs. We performed a comparative analysis between highland and lowland Tibetan pigs using genotyping data to investigate their genetic differences. Our results showed that immune-related genes were selected in highland Tibetan pigs, whereas genes associated with reproduction, growth and development, and blood pressure regulation were selected in lowland Tibetan pigs. These findings will contribute to research into the genetic basis of environmental adaptation and important economic traits in Tibetan pigs and provide genetic targets for breeding programs to improve Tibetan pigs. Tibetan pigs are indigenous to the Qinghai–Tibet Plateau and have been the subject of extensive genomic research primarily focused on their adaptation to high altitudes. However, genetic modifications associated with their response to low-altitude acclimation have not been thoroughly explored. To investigate the genetic basis underlying the low-altitude acclimation of Tibetan pigs, we generated and analyzed genotyping data of Tibetan pigs that inhabit high-altitude regions (average altitude 4000 m) and Tibetan pigs that have inhabited nearby low-altitude regions (average altitude 500 m) for approximately 20 generations. We found that the highland and lowland Tibetan pigs have distinguishable genotype and phenotype variations. We identified 46 and 126 potentially selected SNPs associated with 29 and 56 candidate genes in highland and lowland Tibetan pigs, respectively. Candidate genes in the highland Tibetan pigs were involved in immune response (NFYC and STAT1) and radiation (NABP1), whereas candidate genes in the lowland Tibetan pigs were related to reproduction (ESR2, DMRTA1, and ZNF366), growth and development (NTRK3, FGF18, and MAP1B), and blood pressure regulation (CARTPT). These findings will help to understand the mechanisms of environmental adaptation in Tibetan pigs and offer valuable information into the genetic improvement of Tibetan pigs pertaining to low-altitude acclimation and economic traits. [ABSTRACT FROM AUTHOR]

Atrial fibrillation, one of the most common persistent cardiac arrhythmias globally, is known for its rapid and irregular atrial rhythms. This study integrates the temporal convolutional network (TCN) and residual network (ResNet) frameworks to effectively classify atrial fibrillation in single-lead ECGs, thereby enhancing the application of neural networks in this field. Our model demonstrated significant success in detecting atrial fibrillation, with experimental results showing an accuracy rate of 97% and an F1 score of 87%. These figures indicate the model's exceptional performance in identifying both majority and minority classes, reflecting its balanced and accurate classification capability. This research offers new perspectives and tools for diagnosis and treatment in cardiology, grounded in advanced neural network technology. [ABSTRACT FROM AUTHOR]

Fu tea is receiving increasing attention for its specific aroma, flavor, and dramatic functional benefits. Herein, we explored the effects and underlying mechanisms of Fu loose tea (FLT), Fu brick tea (FBT), and diet pills (orlistat) on a high-fat diet (HFD)-induced obesity. The results indicated that FLT and FBT administration effectively inhibited weight gain, glucose metabolic dysregulation, fat accumulation in organs, hepatic and kidney injury, and oxidative stress induced by HFD. Additionally, FLT and FBT treatments improved the lipid profiles and reduced the production of proinflammatory cytokines by regulating the expression levels of lipid metabolism- and inflammation-related genes. Furthermore, FLT and FBT ameliorated the gut microbiota dysbiosis in HFD-mice in a dose-dependent relationship by increasing the abundance of family Verrucomicrobiaceae and genus Akkermansia and Turicibacter and simultaneously reducing the abundance of family Erysipelotrichaceae and genus Bifidobacterium; in contrast, orlistat did not exert a regulatory effect on gut microbiota similar to FLT and FBT to improve HFD-induced obesity. KEGG analysis of gut microbiota annotation revealed that "metabolism" was the most enriched category. This study further provides a theoretical basis for FLT and FBT to be potential supplements to alleviate diet-induced obesity. [ABSTRACT FROM AUTHOR]

Rapid urbanization has significantly impacted the structure of ecosystem services, accelerating the pressure on natural resources and ecological space. The clarification of the interdependent relationship between new-type urbanization (NTU) and ecosystem services (ESs) has contributed to ecological conservation and high-quality co-ordinated development, in contrast to traditional urbanization. This study focuses on the counties in Shaanxi Province as the research subjects, develops a new urbanization evaluation model for county-level areas, and utilizes Section data at the county level in Shaanxi Province for the years 2000, 2010, and 2020. (To analyze land cover change, we selected four data periods: 1990, 2000, 2010, and 2020, to capture significant spatial trends.) This study employed the comparative analysis method and the Coupled Co-ordination Model (CCDM) to assess the correlation between traditional urbanization and the value of ecosystem services, as well as between new urbanization and the value of ecosystem services. Additionally, the study utilizes the ArcGIS platform to analyze the spatiotemporal characteristics of the two types of urbanization evolution and the spatiotemporal relationship between urbanization and ecosystem co-ordination. The study findings suggest the following: (1) a "low-level coordination" coupling relationship exists between traditional and new urbanization in county-level areas of Shaanxi Province and the value of ecosystem services. (2) The coupling co-ordination of traditional and new urbanization with the value of ecosystem services shows a spread of low-value areas in space, leading to a pattern of low disorder assimilation and significant spatial aggregation. (3) From 2000 to 2020, traditional and new urbanization in various counties of Shaanxi Province consistently exhibited a steady increase, with spatial patterns of "Guanzhong region > Northern Shaanxi region > Southern Shaanxi region". Both types of urbanization have displayed a "low-level coupling" with ecosystem services. (4) During the same period, the new urbanization index in different counties of Shaanxi Province showed a steady increase, demonstrating an advantage of Guanzhong. Its impact on the ecosystem was significantly weaker than that of traditional urbanization. (5) The development of new urbanization can be more effectively co-ordinated with ecosystem services compared to traditional urbanization. However, currently, its co-ordination with the ecosystem service system is relatively poor. The study's results suggest that enhancing new urbanization from multiple dimensions is beneficial for promoting the integrated coherence between urbanization development and ecosystem service systems. [ABSTRACT FROM AUTHOR]

Glass curtain walls (GCWs) have become prevalent in office buildings, owing to their lightweight and modular characteristics. However, their lower thermal resistance, compared to opaque walls, results in increased energy consumption. Incorporating phase-change materials (PCMs) provides a viable solution through which to address the susceptibility of GCWs to external conditions, thus enhancing thermal performance and mitigating energy concerns. This study delves into the influences of the glazing solar heat gain coefficient (SHGC), the glazing heat transfer coefficient (U-value), and PCM thickness on the energy performance of buildings. Using Design Builder (DB) software version 6.1.0.006, a multi-story office building was simulated in different climatic zones in China, covering the climatic characteristics of severe cold, cold, hot summer and warm winter, cold summer and winter, and mild regions. The simulation results quantitatively elucidated the effects of the glazing parameters and the number of PCMs on thermal regulation and energy consumption. A sensitivity analysis identified the glazing SHGC as the most influential factor in energy consumption. Additionally, by employing Response Surface Methodology (RSM), the researchers aimed to achieve a balance between minimal building energy consumption and economic cost, ultimately determining an optimal design solution. The results demonstrated significant energy savings, ranging from 20.16% to 81.18%, accompanied by economic savings, ranging from 15.78% to 79.54%, across distinct climate zones in China. [ABSTRACT FROM AUTHOR]

Fu brick tea (FBT), a product of microbial fermentation from primary dark tea, also known as raw material tea (RMT), has been extensively studied for its functional properties. However, its potential as a staple food supplement for weight loss remains poorly understood. This study compared the weight loss effects of orlistat, traditional plain noodles (NN), and noodles supplemented with varying amounts of RMT (RMTN) and FBT (FBTN), with the aim to elucidate their lipid-reducing effects and underlying mechanisms. Experimental trials on high fat diet fed mice revealed significant weight loss, lipid-lowering, and hypoglycemic effects upon supplementation with orlistat, RMTN, and FBTN. Moreover, supplementation with orlistat, RMTN, and FBTN effectively restored serum and liver-related index levels, mitigating high-fat diet-induced dyslipidemia. Additionally, these supplements ameliorated liver and kidney damage by inhibiting oxidative stress and inflammatory responses. Furthermore, orlistat, RMTN, and FBTN exert their anti-obesity effects primarily by modulating genes associated with lipid metabolism and inflammatory responses and through regulation of the composition and structure of the gut microbiota. Importantly, FBTN demonstrated a significantly stronger lipid-lowering effect compared to RMTN, particularly at higher tea addition ratios. In contrast, NN supplementation exhibited minimal to no weight loss effects. Based on these findings, it could be inferred that FBT holds promise as a staple food supplement to ameliorate high-fat diet-induced obesity and its associated health conditions. [ABSTRACT FROM AUTHOR]

The global concern regarding the monitoring of construction workers' activities necessitates an efficient means of continuous monitoring for timely action recognition at construction sites. This paper introduces a novel approach—the multi-scale graph strategy—to enhance feature extraction in complex networks. At the core of this strategy lies the multi-feature fusion network (MF-Net), which employs multiple scale graphs in distinct network streams to capture both local and global features of crucial joints. This approach extends beyond local relationships to encompass broader connections, including those between the head and foot, as well as interactions like those involving the head and neck. By integrating diverse scale graphs into distinct network streams, we effectively incorporate physically unrelated information, aiding in the extraction of vital local joint contour features. Furthermore, we introduce velocity and acceleration as temporal features, fusing them with spatial features to enhance informational efficacy and the model's performance. Finally, efficiency-enhancing measures, such as a bottleneck structure and a branch-wise attention block, are implemented to optimize computational resources while enhancing feature discriminability. The significance of this paper lies in improving the management model of the construction industry, ultimately aiming to enhance the health and work efficiency of workers. [ABSTRACT FROM AUTHOR]

In this study, Fu-brick tea (FBT) was used for kombucha preparation. The succession of microbial community structures, changes in physicochemical properties, and the volatiles were investigated during the kombucha fermentation. The sequencing analysis showed that Komagataeibacter was the most predominant bacterium. Aspergillus and Zygosaccharomyces were the dominant fungi before fermentation whereas Zygosaccharomyces and Derkella were the dominant fungi after 3 days of fermentation. The physicochemical analysis revealed that acetic acid, glucuronic acid, and polyphenols increased by 10.22 g/L, 0.08 g/L, and 177.40 mg/L, respectively, by the end of fermentation. The GC-MS analysis showed that a total of 49 volatile compounds were detected during the fermentation. Moreover, there were great differences in volatile components among the kombucha samples with different fermentation times. Furthermore, the relevance among microbial community and volatile compounds was evaluated through correlation network analysis. The results suggested that Komagataeibacter, Aspergillus, Zygosaccharomyces, and Dekkera were closely related to the main volatile compounds of FBT kombucha. The results in this study may provide deep understanding for constructing the microbiota and improving the quality of FBT kombucha. [ABSTRACT FROM AUTHOR]

The α-glucosidase inhibitor is of interest to researchers due to its association with type-II diabetes treatment by suppressing postprandial hyperglycemia. Hesperidin is a major flavonoid in orange fruit with diverse biological properties. This paper evaluates the effects of hesperidin on α-glucosidase through inhibitory kinetics, fluorescence quenching, and molecular docking methods for the first time. The inhibition kinetic analysis shows that hesperidin reversibly inhibited the α-glucosidase activity with an IC50 value of 18.52 μM and the inhibition was performed in an uncompetitive type. The fluorescence quenching studies indicate that the intrinsic fluorescence of α-glucosidase was quenched via a static quenching process and only one binding site was present between the hesperidin and α-glucosidase. The interaction between them was spontaneous and mainly driven by hydrogen bonds, as well as hydrophobic forces. Furthermore, the molecular docking results suggest that hesperidin might bond to the entrance or outlet part of the active site of α-glucosidase through a network of five hydrogen bonds formed between hesperidin and the four amino acid residues (Trp709, Arg422, Asn424, and Arg467) of α-glucosidase and the hydrophobic effects. These results provide new insight into the inhibitory mechanisms of hesperidin on α-glucosidase, supporting the potential application of a hesperidin-rich orange product as a hypoglycemic functional food. [ABSTRACT FROM AUTHOR]

Purpose: This study quantifies the gait characteristics of patients with degenerative lumbar scoliosis (DLS) and patients with simple lumbar spinal stenosis (LSS) by means of a three-dimensional gait analysis system, aiming to determine the image of spinal deformity on gait and the correlation between spinal–pelvic parameters and gait characteristics in patients with DLS to assist clinical work. Methods: From June 2020 to December 2021, a total of 50 subjects were enrolled in this study, of whom 20 patients with DLS served as the case group and 30 middle-aged and elderly patients with LSS were selected as the control group according to the general conditions (sex, age, and BMI) of the case group. Spinal–pelvic parameters were measured by full-length frontal and lateral spine films one week before surgery, and kinematics were recorded on the same day using a gait analysis system. Results: Compared to the control group, DLS patients exhibited significantly reduced velocity and cadence; gait variability and symmetry of both lower limbs were notably better in the LSS group than in the DLS group; joint ROM (range of motion) across multiple dimensions was also lower in the DLS group; and correlation analysis revealed that patients with a larger Cobb angle, T1PA, and higher CSVA tended to walk more slowly, and those with a larger PI, PT, and LL usually had smaller stride lengths. The greater the PI-SS mismatch, the longer the patient stayed in the support phase. Furthermore, a larger Cobb angle correlated with worse coronal hip mobility. Conclusions: DLS patients demonstrate distinctive gait abnormalities and reduced hip mobility compared to LSS patients. Significant correlations between crucial spinopelvic parameters and these gait changes underline their potential influence on gait disturbances in DLS. Our study identifies a Cobb angle cut-off of 16.1 as a key predictor for gait abnormalities. These insights can guide personalized treatment and intervention strategies, ultimately improving the quality of life for DLS patients. [ABSTRACT FROM AUTHOR]

Endoscopy is a commonly used clinical method for gastrointestinal disorders. However, the complexity of the gastrointestinal environment can lead to artifacts. Consequently, the artifacts affect the visual perception of images captured during endoscopic examinations. Existing methods to assess image quality with no reference display limitations: some are artifact-specific, while others are poorly interpretable. This study presents an improved cascade region-based convolutional neural network (CNN) for detecting gastrointestinal artifacts to quantitatively assess the quality of endoscopic images. This method detects eight artifacts in endoscopic images and provides their localization, classification, and confidence scores; these scores represent image quality assessment results. The artifact detection component of this method enhances the feature pyramid structure, incorporates the channel attention mechanism into the feature extraction process, and combines shallow and deep features to improve the utilization of spatial information. The detection results are further used for image quality assessment. Experimental results using white light imaging, narrow-band imaging, and iodine-stained images demonstrate that the proposed artifact detection method achieved the highest average precision (62.4% at a 50% IOU threshold). Compared to the typical networks, the accuracy of this algorithm is improved. Furthermore, three clinicians validated that the proposed image quality assessment method based on the object detection of endoscopy artifacts achieves a correlation coefficient of 60.71%. [ABSTRACT FROM AUTHOR]

This paper proposes an underwater broadband target depth estimation method based on the multipath arrival structure in medium and short-range deep-sea environments. The proposed approach involves separating the multipath rays arriving at the vertical line array using the matched filtering technique. The combined beamforming is then applied to the vector hydrophone vertical array (VHVA) to obtain more accurate elevation angle estimates. The components of the interference sound field at different distances are judged, and the signal received by the array is compensated using suitable estimated angles. Finally, the multipath time delay difference is extracted from the pulse peaks of the compensated signal, and the target depth is estimated by establishing the relationship among the multipath time delay difference, the elevation angle and depth. Simulations and experiments demonstrate the effectiveness of this method in reducing the depth estimation bias and avoiding the misjudgment of surface or submerged targets. [ABSTRACT FROM AUTHOR]

Major latex proteins (MLPs) play a key role in plant response to abiotic and biotic stresses. However, little is known about this gene family in tomatoes (Solanum lycopersicum). In this paper, we perform a genome-wide evolutionary characterization and gene expression analysis of the MLP family in tomatoes. We found a total of 34 SlMLP members in the tomato genome, which are heterogeneously distributed on eight chromosomes. The phylogenetic analysis of the SlMLP family unveiled their evolutionary relationships and possible functions. Furthermore, the tissue-specific expression analysis revealed that the tomato MLP members possess distinct biological functions. Crucially, multiple cis-regulatory elements associated with stress, hormone, light, and growth responses were identified in the promoter regions of these SlMLP genes, suggesting that SlMLPs are potentially involved in plant growth, development, and various stress responses. Subcellular localization demonstrated that SlMLP1, SlMLP3, and SlMLP17 are localized in the cytoplasm. In conclusion, these findings lay a foundation for further dissecting the functions of tomato SlMLP genes and exploring the evolutionary relationships of MLP homologs in different plants. [ABSTRACT FROM AUTHOR]

The relationship between spinal alignment, particularly degenerative thoracolumbar kyphosis (DTLK) combined with lumbar spine stenosis (LSS), and paraspinal muscle content remains underexplored. This study aimed to elucidate the characteristics of paraspinal muscle distribution in DTLK patients and its association with lumbar lordosis (LL) and body mass index (BMI). Methods: A case–control study was conducted comparing 126 patients with DTLK and LSS against 87 control patients. The lumbar crossing indentation value (LCIV) was introduced as a novel measurement for paraspinal muscle content, and its relationship with thoracolumbar kyphosis (TLK), BMI, and LL was assessed. Results: LCIV in DTLK patients was found to be lower than in the control group, with a progressive increase from the upper to lower lumbar spine. In the control group, paraspinal muscle content was observed to increase with age and BMI, and LCIV was higher in males. However, the DTLK group showed no gender difference. LCIV in the DTLK group was more pronounced in patients with increased LL. The degree of TLK was not influenced by BMI but was associated with the content of the paravertebral muscle. Conclusions: Paraspinal muscle content, as measured by LCIV, is significantly associated with DTLK and LSS. The study emphasizes the importance of considering paraspinal muscle health in DTLK patients and offers valuable insights for diagnosis and therapeutic interventions. [ABSTRACT FROM AUTHOR]

The variation of mutual inductance and load parameters will affect the transmission power and efficiency of the inductive power transfer (IPT) system. The identification of mutual inductance and load parameters is an essential part of establishing a stable and reliable IPT system. This paper presents a joint identification method of load and mutual inductance for the LCC-S IPT system, which does not require the establishment of primary and secondary communication and related control. Firstly, the resistance-inductance characteristics of the equivalent load of the rectifier are analyzed by simulation, and then the rectifier and system load are equivalent to the circuit model of resistance and inductance in series. Secondly, the characteristics of the reflected impedance are analyzed, and the functional relationship between the transmitter impedance and the rectifier impedance is established by using the ratio of the real part to the imaginary part of the reflected impedance, which realizes the decoupling of the load and the mutual inductance. Thirdly, the functional relationship between the equivalent impedance of the rectifier and the load resistance of the system is obtained by data fitting. Then, the equations of the above two functional relationships are combined. By measuring the voltage of the parallel compensation capacitor at the transmitting side, the current of the transmitting coil and the phase difference between the two, the battery load can be solved first, and then the mutual inductance can be calculated, so that the high-precision identification of the load and mutual inductance can be realized. Finally, an experimental platform of the LCC-S IPT system is built for experimental verification. The experimental results show that the maximum identification errors of mutual inductance and load are 5.20% and 5.53%, respectively, which proves that the proposed identification method can achieve high precision identification. [ABSTRACT FROM AUTHOR]

The mammary glands, responsible for milk secretion, are regulated at a local level by various hormones, growth factors, non-coding RNAs, and other elements. Recent research has discovered the presence of lncRNAs in these glands, with suggestions that they may be essential for the maintenance and function of mammary glands. Besides directly controlling the gene and protein expression, lncRNAs are believed to play a significant part in numerous physiological and pathological processes. This study focused on examining the mammary gland tissues of Chinese Holstein cows, to identify and categorize long non-coding RNAs (lncRNAs). The research intended to distinguish lncRNAs in the mammary tissues of Holstein cows and contrast them between lactation and non-lactation periods. In this study, mammary gland tissues were sampled from three Holstein cows in early lactation (n = 3, 30 days postpartum) and non-lactation (n = 3, 315 days postpartum) on a large dairy farm in Jiangsu province. Mammary tissue samples were collected during early lactation and again during non-lactation. In total, we detected 1905 lncRNAs, with 57.3% being 500 bp and 612 intronic lncRNAs. The exon count for lncRNAs varied from 2 to 10. It was observed that 96 lncRNA expressions markedly differed between the two stages, with 83 genes being upregulated and 53 downregulated. Enrichment analysis results revealed that Gene Ontology (GO) analysis was primarily abundant in cellular processes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that target genes were predominantly abundant in metabolic pathways, fatty acid biosynthesis, the immune system, and glycosphingolipid biosynthesis. This study analyzed the expression profile and characteristics of lncRNAs in the mammary gland tissues of Holstein cows during both lactation and non-lactation stages, forming a foundation for further investigation into the functional roles of lncRNAs in Holstein cows throughout lactation. [ABSTRACT FROM AUTHOR]

Oxygen level is an important environmental factor affecting the circadian rhythm. However, little is known about the molecular mechanism by which clock genes regulate the circadian rhythm in fish under hypoxia. To explore changes in the transcription and expression of clock genes and related molecular regulatory mechanisms in pearl gentian grouper under hypoxia, liver transcriptome data were analyzed after exposure to acute hypoxic stress (dissolved oxygen 0.5 mg/L) for 1, 3, 6, and 9 h. miR-210 and m0044-5p inhibited the expression of period3 (per3) and casein kinase 1 delta b (csnk1db) in the core loop of the circadian clock, respectively. The nuclear receptor subfamily 1 group d member 1 (nr1d1) and RAR-related orphan receptor b (rorb) genes in the auxiliary loop were jointly up-regulated by three miRNAs (miR-144-3p/5p, miR-361-5p, and miR-133) and the transcription factor nuclear receptor subfamily 1 group d member 2 (Nr1d2). The pearl gentian grouper maintains the stability of circadian clock systems and normal physiological metabolism under hypoxic stress by regulating the transcriptional expression of these genes via miRNAs and transcription factors to improve hypoxic tolerance. These findings provide important basic data for future research on hypoxic tolerance in pearl gentian grouper and provide new insights into the interaction between hypoxia and the circadian rhythm in fish. [ABSTRACT FROM AUTHOR]

Simple Summary: In this study, the transcriptomic response of Trachinotus ovatus liver was observed against high-temperature stress. Through differential expression and short time-series expression miner (STEM) analyses, some high-temperature-related genes and biological pathways were screened, which were mainly related to protein balance, hypoxia adaptation, and energy metabolism. Our results suggest that protein dynamic balance and function, hypoxia adaptation, and energy metabolism transformation are crucial in response to acute high-temperature stress. These results contribute to understanding the molecular response mechanism of T. ovatus under acute heat stress and provide novel insights into the selection and breeding of heat-tolerant cultivars and the high-quality development of aquaculture. Trachinotus ovatus is a major economically important cultured marine fish in the South China Sea. However, extreme weather and increased culture density result in uncontrollable problems, such as increases in water temperature and a decline in dissolved oxygen (DO), hindering the high-quality development of aquaculture. In this study, liver transcriptional profiles of T. ovatus were investigated under acute high-temperature stress (31 °C and 34 °C) and normal water temperature (27 °C) using RNA sequencing (RNA-Seq) technology. Differential expression analysis and STEM analysis showed that 1347 differentially expressed genes (DEGs) and four significant profiles (profiles 0, 3, 4, and 7) were screened, respectively. Of these DEGs, some genes involved in heat shock protein (HSPs), hypoxic adaptation, and glycolysis were up-regulated, while some genes involved in the ubiquitin-proteasome system (UPS) and fatty acid metabolism were down-regulated. Our results suggest that protein dynamic balance and function, hypoxia adaptation, and energy metabolism transformation are crucial in response to acute high-temperature stress. Our findings contribute to understanding the molecular response mechanism of T. ovatus under acute heat stress, which may provide some reference for studying the molecular mechanisms of other fish in response to heat stress. [ABSTRACT FROM AUTHOR]

Savalani hairtail Lepturacanthus savala is a widely distributed fish along the Indo-Western Pacific coast, and contributes substantially to trichiurid fishery resources worldwide. In this study, the first chromosome-level genome assembly of L. savala was obtained by PacBio SMRT-Seq, Illumina HiSeq, and Hi-C technologies. The final assembled L. savala genome was 790.02 Mb with contig N50 and scaffold N50 values of 19.01 Mb and 32.77 Mb, respectively. The assembled sequences were anchored to 24 chromosomes by using Hi-C data. Combined with RNA sequencing data, 23,625 protein-coding genes were predicted, of which 96.0% were successfully annotated. In total, 67 gene family expansions and 93 gene family contractions were detected in the L. savala genome. Additionally, 1825 positively selected genes were identified. Based on a comparative genomic analysis, we screened a number of candidate genes associated with the specific morphology, behaviour-related immune system, and DNA repair mechanisms in L. savala. Our results preliminarily revealed mechanisms underlying the special morphological and behavioural characteristics of L. savala from a genomic perspective. Furthermore, this study provides valuable reference data for subsequent molecular ecology studies of L. savala and whole-genome analyses of other trichiurid fishes. [ABSTRACT FROM AUTHOR]

Xanthomonas oryzae pv. oryzae (Xoo) is a causative agent of rice bacterial blight (BB). In 2020–2022, BB re-emerged, and there was a break out in the Yangtze River area, China. The pandemic Xoo strain, LA20, was isolated and identified from cultivar Quanyou1606 and demonstrated to be the Chinese R9 Xoo strain, which is able to override the widely adopted xa5-, Xa7- and xa13-mediated resistance in rice varieties in Yangtze River. Here, we report the complete genome of LA20 by PacBio and Illumina sequencing. The assembled genome consists of one circular chromosome of 4,960,087 bp, sharing 99.65% sequence identity with the traditional representative strain, YC11 (R5), in the Yangtze River. Comparative genome analysis of LA20 and YC11 revealed the obvious variability in Tal genes (the uppermost virulence determinants) in numbers and sequences. Particularly, six Tal genes were only found in LA20, but not in YC11, among which Tal1b (pthXo1)/Tal4 (pthXo6), along with the lost one, pthXo3 (avrXa7), might be the major factors for LA20 to overcome xa5-, Xa7- and xa13-mediated resistance, thus, leading to the resurgence of BB. This complete genome of the new pandemic Xoo strain will provide novel insights into pathogen evolution, the traits of pathogenicity on genomic level and the epidemic disease status in China. [ABSTRACT FROM AUTHOR]

From a configurational perspective, this study aims to explain how clusters drive firm performance in the regional innovation system by considering the relationship between cluster and firm performance as causal complexity. Using an original dataset comprising 292 Chinese firms in strategic emerging industries (SEIs), this study employs a fuzzy set qualitative comparative analysis (fsQCA) to investigate the conjunction effects of interorganizational dependence, network embeddedness, and ambidextrous innovation on cluster firms' performance. The results showed that the fsQCA method uncovers causal combinations of these cluster factors that lead to high performance. These configurations imply two alternative pathways to enhance performance, where exploitative innovation is identified as the core causal condition. [ABSTRACT FROM AUTHOR]

The physicochemical properties of ligand-coated nanoparticles make them superior adsorbents for heavy metals from water. In this study, we investigate the adsorption potential of novel polyacrylic-co-maleic-acid-coated magnetite nanoparticles (PAM@MNP) to remove Pb2+ and Cu2+ from an aqueous solution. We argue that modifying the surface of MNP with PAM enhances the physicochemical stability of MNP, improving its ability to remove heavy metals. The adsorption kinetics data show that PAM@MNP attained sorption equilibrium for Pb2+ and Cu2+ after 60 min. The kinetics data are fitted accurately by the pseudo-first-order kinetic model. The calculated Langmuir adsorption capacities are 518.68 mg g−1 and 179.81 mg g−1 for Pb2+ and Cu2+, respectively (2.50 mmol g−1 and 2.82 mmol g−1 for Pb2+ and Cu2+, respectively). The results indicate that PAM@MNP is a very attractive adsorbent for heavy metals and can be applied in water remediation technologies. [ABSTRACT FROM AUTHOR]

Food allergies are a serious food safety and public health issue. Soybean, dairy, aquatic, poultry, and nut products are common allergens inducing allergic reactions and adverse symptoms such as atopic dermatitis, allergic eczema, allergic asthma, and allergic rhinitis. Probiotics are assumed as an essential ingredient in maintaining intestinal microorganisms' composition. They have unique physiological roles and therapeutic effects in maintaining the mucosal barrier, immune function, and gastrointestinal tract, inhibiting the invasion of pathogenic bacteria, and preventing diarrhea and food allergies. Multiple pieces of evidence reveal a significant disruptive effect of probiotics on food allergy pathology and progression mechanisms. Thus, this review describes the allergenic proteins as an entry point and briefly describes the application of probiotics in allergenic foods. Then, the role of probiotics in preventing and curing allergic diseases by regulating human immunity through intestinal flora and intestinal barrier, modulating host immune active cells, and improving host amino acid metabolism are described in detail. The anti-allergic role of probiotics in the function and metabolism of the gastrointestinal tract has been comprehensively explored to furnish insights for relieving food allergy symptoms and preventing food allergy. [ABSTRACT FROM AUTHOR]

Free-running InGaAs/InP single-photon avalanche photodiodes (SPADs) typically operate in the active-quenching mode, facing the problems of long dead time and large timing jitter. In this paper, we demonstrate a 1-GHz gated InGaAs/InP SPAD with the sinusoidal gating signals asynchronous to the incident pulsed laser, enabling free-running single-photon detection. The photon-induced avalanche signals are quenched within 1 ns, efficiently reducing the SPAD's dead time and achieving a count rate of up to 500 Mcount/s. However, the timing jitter is measured to be ~168 ps, much larger than that of the SPAD with synchronous gates. We adjust the delay between the gating signals and the incident pulsed laser to simulate the random arrival of the photons, and record the timing jitter, respectively, to figure out the cause of the jitter deterioration. In addition, the effects of the incident laser power and working temperature of the APD on the time resolution have been investigated, broadening the applications of the GHz gated free-running SPAD in laser ranging and imaging, fluorescence spectroscopy detection and optical time-domain reflectometry. [ABSTRACT FROM AUTHOR]

Simple Summary: While the prognosis of hormone receptor-positive (HR+) breast cancer has been significantly improved, tamoxifen resistance remains a challenge in the treatment of HR+ breast cancer. This study identified that tRF-16-K8J7K1B, a novel small ncRNA derived from the 3′-end of tRNAAla-TGC, was highly expressed in tamoxifen-resistant cells compared to parental cells. Moreover, extracellular tRF-16-K8J7K1B confers tamoxifen resistance via incorporation into exosomes and then degrades the expression of apoptosis-related proteins, reducing the proportion of drug-induced cell apoptosis. Therefore, we propose that exosomal tRF-16-K8J7K1B could be a potential predictive biomarker and therapeutic target for overcoming tamoxifen resistance. Tamoxifen resistance remains a challenge in hormone receptor-positive (HR+) breast cancer. Recent evidence suggests that transfer ribonucleic acid (tRNA)-derived fragments play pivotal roles in the occurrence and development of various tumors. However, the relationship between tRNA-derived fragments and tamoxifen resistance remains unclear. In this study, we found that the expression of tRF-16-K8J7K1B was upregulated in tamoxifen-resistant cells in comparison with tamoxifen-sensitive cells. Higher levels of tRF-16-K8J7K1B were associated with shorter disease-free survival in HR+ breast cancer. Overexpression of tRF-16-K8J7K1B promotes tamoxifen resistance. Moreover, extracellular tRF-16-K8J7K1B could be packaged into exosomes and could disseminate tamoxifen resistance to recipient cells. Mechanistically, exosomal tRF-16-K8J7K1B downregulates the expression of apoptosis-related proteins, such as caspase 3 and poly (ADP-ribose) polymerase, by targeting tumor necrosis factor-related apoptosis-inducing ligand in receptor cells, thereby reducing drug-induced cell apoptosis. Therapeutically, the inhibition of exosomal tRF-16-K8J7K1B increases the sensitivity of breast cancer cells to tamoxifen in vivo. These data demonstrate that exosomal tRF-16-K8J7K1B may be a novel therapeutic target to overcome tamoxifen resistance in HR+ breast cancer. [ABSTRACT FROM AUTHOR]

Breeding crops with enhanced immunity is an effective strategy to reduce yield loss caused by pathogens. The constitutive expresser of pathogenesis-related genes (cpr5) mutant shows enhanced pathogen resistance but retarded growth; thus, it restricts the application of cpr5 in breeding crops with disease resistance. Reactive oxygen species (ROS) play important roles in plant growth and defense. In this study, we determined that the cpr5 mutant exhibited excessive ROS accumulation. However, the mutation of respiratory burst oxidase homolog D (RBOHD), a reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase responsible for the production of ROS signaling in plant immunity, did not suppress excessive ROS levels in cpr5. Furthermore, the cpr5 mutant showed low levels of ascorbate peroxidase 1 (APX1), an important cytosolic ROS-scavenging enzyme. APX1 overexpression in the cpr5 background removed excessive ROS and restored the pleiotropic growth phenotype. Notably, APX1 overexpression did not reduce the resistance of cpr5 mutant to virulent strain Pseudomonas syringae pv. tomato (Pst) DC3000 and avirulent strain Pst DC3000 (avrRpt2). These results suggest that the removal of excessive ROS by APX1 overexpression restored the cpr5 growth phenotype while conserving pathogen resistance. Hence, our study provides a theoretical and empirical basis for utilizing CPR5 in the breeding of crops with disease resistance by effective oxidative stress management via APX1 expression. [ABSTRACT FROM AUTHOR]