Search

Organic acids are a key active component of Pinellia ternata (Thunb.) Breit, and their concentration is closely associated with the quality of P. ternata. Developing an efficient and rapid method for detecting organic acids can offer a valuable technology for real-time assessment of P. ternata quality. In this work, a high-performance liquid chromatography coupled to triple quadrupole mass spectrometry (HPLC-QqQ-MS/MS) approach, combining the optimization of extraction conditions using response surface methodology (RSM), was developed for the simultaneous quantitative analysis of six organic acids in P. ternata. The optimal extraction conditions for organic acids in P. ternata were ultrasonic extraction with a solid–liquid ratio of 1:50, ultrasonic time of 60 min, and extraction temperature of 55 °C. Multiple-reaction monitoring (MRM) scanning was employed for quantification using HPLC-QqQ-MS/MS in negative ionization mode through a single run of 10 min, and the limit of detection (LLOD) and limit of quantification (LLOQ) for organic acids were as low as 0.138 ng/mL and 0.614 ng/mL, respectively. The relative standard deviations (RSDs) of reproducibility, precision, and stability were all lower than 5.0%, and recovery rates were 97.75–107.14%, with RSDs < 5.0%. Finally, this method was successfully applied to determine the investigated organic acids in 12 production regions of P. ternata, revealing significant differences between different production areas. This indicates that the optimized method is suitable for further accurate investigations of organic acids of P. ternata. [ABSTRACT FROM AUTHOR]

Mulberry fruits are rich in bioactive components renowned for their antioxidant properties and potential health benefits. This study thoroughly investigated the impact of cultivation modes on the phenological stages, yield, bioactive components, and antioxidant activity of two mulberry cultivars, Yueshen Dashi (YS-DS) and Xinjiang Baisang (XJ-BS). Notably, greenhouse cultivation led to earlier phenology and shorter fruit development maturation durations compared to field cultivation. Despite a decrease in fruit production and firmness, the greenhouse-grown mulberries exhibited higher individual fruit fresh weight. The content of bioactive components, encompassing anthocyanins, polyphenols, flavonoids, and vitamin C, and of antioxidant activity (measured in the FRAP and DPPH radical scavenging assays) was found to be lower in the greenhouse-grown mulberries than in those cultivated in the field. The contents of total polyphenols and flavonoids showed robust positive correlations in the FRAP and DPPH radical scavenging assays, which suggests that the antioxidant activity of mulberry fruit might be primarily attributable to the bioactive components of total polyphenols and flavonoids. Interestingly, the sugar content and hydroxyl radical scavenging activity (HRSA) displayed an inverse relationship between the two cultivars in the greenhouse versus field conditions. The multivariate analysis highlighted distinct patterns for different cultivars under varying cultivation modes. This study underscores the potential to enhance bioactive components and antioxidant activity through effective manipulation of climate conditions, thereby unlocking the full nutritional potential of mulberry fruits on a large scale in greenhouse environments. [ABSTRACT FROM AUTHOR]

The plastic region of piles under seismic loads is a crucial concern in seafloor improvement design. This paper establishes a physical model of the sand compaction pile-immersed tunnel–water pressure system. This research studies pile arrangements that minimize the sand compaction pile plastic region under seismic loads. The experiments were validated through numerical simulations. The results show that "X-shaped" and rectangular pile groups increase the Energy Residual Index (ERI) due to differences in pile spacing and the instability of the quadrilateral prism damping units formed by piles and soil. In this scenario, piles are limited to heavy and mild plastic regions, with boundary depths at L = 2.25 D and L = 2.08 D (L represents the pile length, and D is the pile diameter). Furthermore, increased water pressure amplifies the structural resonance injury, increasing ERI. In conjunction with the soil, hexagonal pile groups create triangular prism damping units that counteract seismic wavefronts. The total kinetic energy and strain energy of the piled foundation are lower than those of the "X-shaped" and rectangular pile groups. The boundaries between the heavy plastic region, the moderate plastic region, and the mild plastic region are located at depths of L = 4 D and L = 8 D, respectively. This study also reveals that a top-heavy mass distribution in the structure leads to maximum deformation in the heavy plastic region. Pile–soil damping units primarily operate within the moderate plastic region. [ABSTRACT FROM AUTHOR]

Featured Application: This paper focuses on the redevelopment of the ABAQUS finite element software using the improved Mohr–Coulomb model, which enables the consideration of soil anisotropy. The findings of this research provide valuable theoretical guidance for on-site construction design, helping to prevent potential hazards arising from incomplete considerations during construction. It is an economical way to use the pile-supported embankment for the construction of the embankment over soft soil. The combined use of piles and reinforcement effectively reduces the differential settlement of the embankment surface. However, the previous analysis of embankment stress and settlement did not take into account the anisotropy in the embankment filler. In this paper, the UMAT subroutine is developed by using the material subroutine interface in ABAQUS 2016 finite element software. The anisotropy of soil cohesion and friction angle has been incorporated into the Mohr–Coulomb yield criterion so that it can consider the anisotropy of soil. The accuracy of the anisotropic yield criterion in this paper is verified by an ABAQUS source program and related engineering examples. It is found that the anisotropy value of soil cohesion is inversely proportional to the stress ratio on the pile–soil interface while being directly proportional to the tensile stress applied to the geogrid. The results show that the anisotropy of the friction angle decreases with the soil arching effect but increases by 23.1% with the tensile stress on the geogrid. The position of the settlement plane remains relatively constant at 2.3 m as the friction angle anisotropy coefficient increases. These research results provide valuable theoretical guidance for on-site construction design. [ABSTRACT FROM AUTHOR]

China faces challenges of food security and sustainable agricultural production. However, current studies rarely address the spatial distribution patterns of water consumption and carbon emissions. We studied the irrigation water use efficiency and carbon emission differences of crops in arid areas and their spatial distribution using wheat and maize, two major food crops in the middle reaches of the Heihe River, as examples. Furthermore, we have optimized low-carbon cropping of crops under the multiple objectives of water conservation and economic development. The results show that: (1) The carbon emissions per unit of water consumption for maize are 0.03 × 10−6 t mm−1 and 0.49 × 10−6 t mm−1 for wheat. Irrigation water consumption per unit yield is 515.6 mm t−1 for maize and 426.7 mm t−1 for wheat. (2) The spatial distribution patterns of irrigation water consumption were opposites for maize and wheat. The former has lower irrigation water consumption in the planting area upstream of the Heihe River and higher in the lower reaches. In contrast, the pattern of wheat irrigation is the opposite. (3) After optimizing the cropping mix for both crops, the area planted with wheat should be reduced to 59% of the current size, while maize should be expanded to 104%. The results of the research hold immense importance in guiding the future grain crop planting patterns for water-saving agriculture and low-carbon agriculture development in arid zones worldwide, aligning with the United Nations' Sustainable Development Goals. [ABSTRACT FROM AUTHOR]

During recent years, microrobots have drawn extensive attention owing to their good controllability and great potential in biomedicine. Powered by external physical fields or chemical reactions, these untethered microdevices are promising candidates for in vivo complex tasks, such as targeted delivery, imaging and sensing, tissue engineering, hyperthermia, and assisted fertilization, among others. However, in clinical use, the biodegradability of microrobots is significant for avoiding toxic residue in the human body. The selection of biodegradable materials and the corresponding in vivo environment needed for degradation are increasingly receiving attention in this regard. This review aims at analyzing different types of biodegradable microrobots by critically discussing their advantages and limitations. The chemical degradation mechanisms behind biodegradable microrobots and their typical applications are also thoroughly investigated. Furthermore, we examine their feasibility and deal with the in vivo suitability of different biodegradable microrobots in terms of their degradation mechanisms; pathological environments; and corresponding biomedical applications, especially targeted delivery. Ultimately, we highlight the prevailing obstacles and perspective solutions, ranging from their manufacturing methods, control of movement, and degradation rate to insufficient and limited in vivo tests, that could be of benefit to forthcoming clinical applications. [ABSTRACT FROM AUTHOR]

Artemisia argyi Levl. et Van (A. argyi) is a traditional medicinal plant, which is widely used in health, food and medicine. Thujone is an important cyclic monoterpene derivative in the volatile oil of A. argyi leaves with multiple efficacy. Although the thujone synthetic pathway has been preliminarily analyzed in very few species, genes related to the thujone content in A. argyi leaves remain largely unknown. In this study, we identify candidate genes involved in the synthesis and regulation of thujone content in A. argyi leaves by the comparative transcriptome analysis of two group materials with high and low thujone content. A total of 89 candidate genes related to thujone content are identified including one gene involved in the mevalonate pathway, three genes involved in the methylerythritol phosphate pathway, 19 genes involved in the metabolic process from geranyl pyrophosphate to thujone (four b-terpene synthase, five cytochrome P450, five dehydrogenase, and five reductase-encoding genes) and 66 transcription factor-encoding genes. Taken together, our results provide valuable gene resources for further analyzing the synthetic and regulatory pathway of thujone in A. argyi. [ABSTRACT FROM AUTHOR]

Mulberry (Morus L.; Moraceae; Rosales) is an economically important tree with a long history in China and valued for its rich nutrient, flavor content, medicinal value and useful ecological functions. Morus species are widely distributed in Asia, Europe, North and South America and Africa and exhibit obvious differences in morphological characteristics and phenological stages due to being distributed in diverse climate zones. The morphological description of the phenological stages of black mulberry (Morus nigra L.) has been established in Mediterranean climates and this study extended the BBCH scale for application in different Morus species, especially for mulberry trees grown in subtropical monsoon climates. In this study, we used the BBCH (Biologische Bundesanstalt, Bundessortenamt and CHemische Industrie) scale to describe in detail the phenological growth stages for Morus species in Wuhan, China (a subtropical monsoon climate). Based on this general scale, eight principal stages, i.e., bud, leaf and shoot development stage, inflorescence emergence stage, flowering stage, fruit development stage, fruit maturation stage, senescence and beginning of dormancy stage, were described. We provide photographic images of some primary and secondary developmental stages to better define and standardize morphological characteristics and phenological descriptions of these mulberries (Morus spp.). In addition, because the color of mulberry fruit varies from white, purple, black, or pink to red at maturity, with mostly monosexual flowers, we have also presented detailed descriptions of morphological traits of flower sexuality and fruit color among these four species. In addition, two late phenological germplasms (late bud sprouting associated with late blooming and late fruit ripening) were screened out according to the BBCH scale. Overall, this study will contribute to advance the field of mulberry breeding and implementation of agronomic practices and facilitate convenient communication between mulberry cultivators and researchers in different areas. [ABSTRACT FROM AUTHOR]

Simple Summary: Androgen receptor splice variant 7 (AR-V7) has always been considered a key driver for triggering enzalutamide resistance of castration-resistant prostate cancer (CRPC). In recent years, both the homeostasis of AR-V7 protein and AR-V7's relationship with LncRNAs have gained great attention with in-depth studies. Starting from protein stability and LncRNA, the paper discusses and summarizes the mechanisms and drugs that affect the CRPC patients' sensitivity to enzalutamide by regulating the protein or transcriptional stability of AR-V7, hoping to provide therapeutic ideas for subsequent research to break through the CRPC therapeutic bottleneck. Prostate cancer (PCa) has the second highest incidence of malignancies occurring in men worldwide. The first-line therapy of PCa is androgen deprivation therapy (ADT). Nonetheless, most patients progress to castration-resistant prostate cancer (CRPC) after being treated by ADT. As a second-generation androgen receptor (AR) antagonist, enzalutamide (ENZ) is the current mainstay of new endocrine therapies for CRPC in clinical use. However, almost all patients develop resistance during AR antagonist therapy due to various mechanisms. At present, ENZ resistance (ENZR) has become challenging in the clinical treatment of CRPC. AR splice variant 7 (AR-V7) refers to a ligand-independent and constitutively active variant of the AR and is considered a key driver of ENZR in CRPC. In this review, we summarize the mechanisms and biological behaviors of AR-V7 in ENZR of CRPC to contribute novel insights for CRPC therapy. [ABSTRACT FROM AUTHOR]

Although STK38 (serine-threonine kinase 38) has been proven to play an important role in cancer initiation and progression based on a series of cell and animal experiments, no systemic assessment of STK38 across human cancers is available. We firstly performed a pan-cancer analysis of STK38 in this study. The expression level of STK38 was significantly different between tumor and normal tissues in 15 types of cancers. Meanwhile, a prognosis analysis showed that a distinct relationship existed between STK38 expression and the clinical prognosis of cancer patients. Furthermore, the expression of STK38 was related to the infiltration of immune cells, such as NK cells, memory CD4+ T cells, mast cells and cancer-associated fibroblasts in a few cancers. There were three immune-associated signaling pathways involved in KEGG analysis of STK38. In general, STK38 shows a significant prognostic value in different cancers and is closely associated with cancer immunity. [ABSTRACT FROM AUTHOR]

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a secretory serine protease synthesized primarily by the liver. It mainly promotes the degradation of low-density lipoprotein receptor (LDL-R) by binding LDL-R, reducing low-density lipoprotein cholesterol (LDL-C) clearance. In addition to regulating LDL-R, PCSK9 inhibitors can also bind Toll-like receptors (TLRs), scavenger receptor B (SR-B/CD36), low-density lipoprotein receptor-related protein 1 (LRP1), apolipoprotein E receptor-2 (ApoER2) and very-low-density lipoprotein receptor (VLDL-R) reducing the lipoprotein concentration and slowing thrombosis. In addition to cardiovascular diseases, PCSK9 is also used in pancreatic cancer, sepsis, and Parkinson's disease. Currently marketed PCSK9 inhibitors include alirocumab, evolocumab, and inclisiran, as well as small molecules, nucleic acid drugs, and vaccines under development. This review systematically summarized the application, preclinical studies, safety, mechanism of action, and latest research progress of PCSK9 inhibitors, aiming to provide ideas for the drug research and development and the clinical application of PCSK9 in cardiovascular diseases and expand its application in other diseases. [ABSTRACT FROM AUTHOR]

We herein report on the synthesis of a pentacyclic triterpene functionalized through derivation of betulinic acid with hydroxybenzotriazole. The compound was fully characterized by proton (1H-NMR), carbon-13 (13C-NMR), heteronuclear single quantum coherence (HSQC) and distortionless enhancement by polarization transfer (DEPT-135 and DEPT-90) nuclear magnetic resonance. Ultraviolet (UV), and Fourier-transform infrared (FTIR) spectroscopies as well as and high-resolution mass spectrometry (HRMS) were also adopted. Computational studies were conducted to foresee the interactions between compound 3 and phosphodiesterase 9, a relevant target in the field of neurodegenerative diseases. Additionally, preliminary calculation of physico-chemical descriptors was performed to evaluate the drug-likeness of compound 3. [ABSTRACT FROM AUTHOR]

Hypsizygus marmoreus is a representative edible mushroom with low-temperature fruiting after a long postripening (LFLP). Clarifying the mechanism of LFLP and applying a rigorous low-temperature-limited process will optimize the mushroom cultivation process. This study performed an integrative multi-omics analysis of the molecular mechanism of LFLP in combination with genetic, physiological, and cultivation confirmation. The results showed that the amino acid content was increased during LFLP, mainly arginine. pH analysis showed acidification in the postripening stage and alkalization in the substrates of the reproductive growth stage. An enzyme activity test confirmed the increased enzyme activity of arginase and citrate synthase in the postripening stage. Weighted gene coexpression network analysis of the transcriptome and metabolomics indicated that pH variation is correlated mainly with changes in citrate and arginine. Multi-omics reveals a straightforward way of providing enriched materials for amino acid biosynthesis, namely, synergistically elevating citric acid and arginine through enhanced activity of the arginine synthesis branch pathway in the citrate cycle. Our study confirmed that GCN2 mediated metabolic adaptation by enhancing protein translation, highlighting its regulatory role during LFLP. Exogenously added citric acid and arginine shortened the postripening period by 10 days and increased the fruiting body yield by 10.2~15.5%. This research sheds light on the molecular mechanism of LFLP in H. marmoreus and highlights the promising application of nutrient accumulation in high-efficiency cultivation. [ABSTRACT FROM AUTHOR]

In order to explore the relationship between the filling surface angle and the dynamic characteristics of the filling body under impact load, this paper uses the Hopkinson pressure bar (SHPB) test system to study the influence of different filling surface angles, different cement–sand ratios and different strain rates on the dynamic peak strength, dynamic strength growth factor and failure mode of the filling body. The results show that with the increase in the average strain rate, the dynamic peak strength and dynamic strength growth factor of the layered filling body increase gradually. With the increase in the filling surface angle, the static and dynamic peak strength of the layered filling body decreases gradually, but there is no obvious change law linking the dynamic strength growth factor and filling surface angle. According to the failure mode analysis and the LS-DYNA numerical simulation results for the layered filling body, with the increase in the filling surface angle, the failure mode of the layered filling body changes from splitting failure under tension to shear failure. The research results in this paper can provide theoretical support for the stability of underground layered filling bodies. [ABSTRACT FROM AUTHOR]

Tembusu virus (TMUV), a highly infectious pathogenic flavivirus, causes severe egg-drop and encephalitis in domestic waterfowl, while the determinants responsible for viral pathogenicity are largely unknown. In our previous studies, virulent strain JXSP2-4 had been completely attenuated by successive passages in BHK-21 cells and the avirulent strain was designated as JXSP-310. Based on the backbone of JXSP2-4, a series of chimeric viruses were generated according to the amino acid substitutions in NS5 and their infectivities were also analyzed in cell cultures and ducklings. The results showed that the viral titers of RNA-dependent RNA polymerase (RdRp) domain-swapped cheimeric mutant (JXSP-310RdRp) in cells and ducklings were both markedly decreased compared with JXSP2-4, indicating that mutations in the RdRp domain affected viral replication. There are R543K and V711A two amino acid substitutions in the RdRp domain. Further site-directed mutagenesis showed that single-point R543K mutant (JXSP-R543K) exhibited similar replication efficacy compared with JXSP2-4 in cells, but the viral loads in JXSP-R543K-infected ducklings were significantly lower than that of JXSP2-4 and higher than JXSP-310RdRp. Surprisingly, the single-point V711A mutation we introduced rapidly reverted. In addition, qRT-PCR and Western blot confirmed that the mutations in the RdRp domain significantly affected the replication of the virus. Taken together, these results show that R543K substitution in the RdRp domain impairs the in vivo growth of TMUV, but sustaining its attenuated infectivity requires the concurrent presence of the V711A mutation. [ABSTRACT FROM AUTHOR]

In this study, brewery wastewater was used as the treatment in exploring the optimal conditions and maximum processing efficiency of the completely mixed anaerobic biofilm reactor (CMABR) under the conditions of hydraulic retention time (HRT) (18 h, 24 h, and 30 h) with a rotational speed (70 rpm, 100 rpm, and 130 rpm) and influent total alkalinity (TA) (20 mmol/L, 25 mmol/L, and 30 mmol/L), which was measured by the response surface methodology (RSM). The results indicated that the maximum chemical oxygen demand (COD) removal ratio was achieved under the following conditions: HRT of 21.42 h, rotational speed of 101.34 rpm, and influent TA of 25.22 mmol/L. Analysis by scanning electron microscope (SEM) showed that the microorganisms were successfully immobilized on the polyurethane fillers before the reactor began operation. High-throughput sequencing indicated that Methanothrix and Methanospirillum were the dominant contributors for COD removal in the CAMBR under these optimum conditions. [ABSTRACT FROM AUTHOR]

Salmonella infection not only causes acute and chronic diseases in poultry flocks, but the infected poultry are among the most important reservoirs for a variety of Salmonella serovars frequently transmitted to humans. This study aimed to investigate the occurrence of Salmonella spp. in local poultry farms in China. Samples (n = 4255), including dead-in-shell embryos, culled day-old-hatchings and 1- to 4-week-old diseased birds, were collected for Salmonella culture from broiler chicken, meat-type duck and pigeon farms in northern China between 2014 and 2018. A total of 103 Salmonella were isolated. S.enterica serovar Enteritidis and S. Typhimurium were the most prevalent serovars, representing 53.4% and 34.9% of the isolates, respectively. Serovar diversity was the highest in ducks, with the S. Apeyeme being isolated for the first time from duck tissues. All isolates were characterized by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). MLST showed that all S. Enteritidis isolates shared the same sequence type (ST11), and Typhimurium showed several rare STs in addition to ST19. In comparison, PFGE showed better discrimination for S. Enteritidis and S. Typhimurium isolates, with nine distinct pulsotypes being observed. The isolates exhibited varying degrees of resistance to 15 tested antimicrobials and identified S. Enteritidis isolates (98.18%) with multiple antimicrobial resistance were a cause for concern. Our data on invasive Salmonella infection in meat-type poultry in local farms can be used to identify sources and factors associated with Salmonella spread in poultry and the associated food chain. [ABSTRACT FROM AUTHOR]

Fracturing wastewater is often highly emulsified, viscous, and has a high chemical oxygen demand (COD), which makes it difficult to treat and recycle. Ferrate(VI) is a green oxidant that has a high redox potential and has been adopted for the efficient oxidation of fracturing wastewater to achieve triple effects: demulsification, visbreaking, and COD removal. Firstly, optimal conditions were identified to build a model for fast and efficient treatment. Secondly, wastewater treatment using ferrate oxidation was investigated via demulsification, visbreaking, and COD removal. Finally, a mechanism for ferrate oxidation was proposed for the three effects using Fourier-transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). The theoretical and experimental data demonstrated that the ferrate oxidation achieved the three desired effects. When ferrate was added, the demulsification efficiency increased from 56.2% to 91.8%, the total viscosity dropped from 1.45 cp to 1.10 cp, and the total removal rate of COD significantly increased to 74.2%. A mechanistic analysis showed that the strongly-oxidizing ferrate easily and efficiently oxidized the O/W interfacial film materials, viscous polymers, and compounds responsible for the COD, which was a promising result for the triple effects. [ABSTRACT FROM AUTHOR]