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Ginkgo biloba L. (Ginkgoacea) contains an abundance of beneficial compounds and has demonstrated positive clinical effects in the management of metabolic syndrome. Recent studies have emphasized its efficacy against type 2 diabetes mellitus (T2DM), including improvements in diabetic nephropathy and retinopathy. Particularly noteworthy are ginkgolic acid analogs, which have shown potential in combating T2DM by inhibiting protein tyrosine phosphatases (PTPs), facilitating glucose uptake, and influencing signaling pathways. In this study, we isolated six derivatives of ginkgolic acid from the MeOH extract of G. biloba leaves with the guidance of liquid chromatography–mass spectrometry (LC/MS). We determined the chemical structures of these isolated compounds as 2-hydroxy-6-(10′-hydroxypentadec-11′(E)-en-1-yl) benzoic acid (1), 2-hydroxy-6-(11′-hydroxypentadec-9′(E)-en-1-yl) benzoic acid (2), 2-hydroxy-6-tridecylbenzoic acid (3), 2-hydroxy-6-pentadecylbenzoic acid (4), 2-hydroxy-6-(12′-hydroxyheptadec-13′(E)-en-1-yl) benzoic acid (5), and 2-hydroxy-6-(11-hydroxyundecyl) benzoic acid (6) using NMR spectroscopic data and LC/MS analysis. To assess their potential for addressing T2DM, we subjected the isolated compounds (1–6) to tests measuring their inhibitory activity against six PTPs: PTPN11, PTPN2, PTP1B, DUSP9, PTPRS, and PTPN9. Among these compounds, compounds 3–5 displayed enzyme inhibition exceeding 90% against all six PTPs. In conclusion, ginkgolic acid derivatives, acting as PTP inhibitors relevant to insulin resistance, hold promise as potential therapeutic candidates for the prevention and treatment of T2DM. [ABSTRACT FROM AUTHOR]

While numerous studies have examined microbial attacks on waterlogged archaeological wood, limited information is available regarding microbial attacks in waterlogged tropical hardwoods submerged in marine environments. In this context, we explored microbial attacks in waterlogged archaeological rosewood (Dalbergia species), a tropical hardwood species that was submerged in the Yellow Sea for approximately 700 years, using various microscopic techniques and next-generation sequencing (NGS) methods. Based on morphological features, Type-I soft rot decay was identified as the main decay type. Most fibers in waterlogged archaeological rosewood studied were gelatinous (G) fibers of tension wood and the mode of soft rot decay differed from fibers without the G-layer. Differences in decay resistance between vessel/axial parenchyma cells and fibers were not obvious. Vestured- and simple pit membranes showed higher decay resistance than vessel and axial parenchyma cell walls, respectively. Microbial community analysis by NGS revealed the dominance of Ascomycota and Basidiomycota in the fungal community. Various bacterial communities were also identified, although no prominent signs of bacterial decay were noted. The identified bacterial communities markedly differed from those reported previously in terms of their composition and abundance. Together, our results offer detailed insights into the microbial types and communities responsible for degrading waterlogged archaeological rosewood, contributing to a better understanding of microbial attacks in tropical hardwoods exposed to marine environments. [ABSTRACT FROM AUTHOR]

The probabilistic uncertainty assessment of geomechanical risk—specifically, caprock failure—attributable to CO2 injection, as presented in a simplified hypothetical geological model, was the focus of this study. Our approach amalgamates the implementation of a multilaminate model, the creation of a response surface model in conjunction with the Box–Behnken sampling design, the execution of associated numerical modeling experiments, and the utilization of Monte Carlo simulations. Probability distributions to encapsulate the inherent variability (elastic and mechanical properties of the caprock and reservoir) and uncertainty in prediction estimates (vertical displacement, total strain, and F value) were employed. Our findings reveal that the Young modulus of the caprock is a key factor controlling equivalent total strain but is insufficient as a stand-alone indicator of caprock integrity. It is confirmed that the caprock can accommodate significant deformation without failure, if it possesses a low Young's modulus and high mechanical strength properties, such as the friction angle and uniaxial compressive strength. Similarly, vertical displacement was found to be an unreliable indicator for caprock integrity, as caprock failure can occur across a broad spectrum of vertical displacements, particularly when both the Young modulus and mechanical strength properties have wide ranges. This study introduces the F value as the most dependable indicator for caprock failure, although it is a theoretical attribute (the shortest distance between the Mohr circle and the nearest failure envelope used to measure the sensitivity to failure) and not physically measurable in the field. Deviatoric stress levels were found to vary based on stress regimes, with the maximum levels observed under extensive and compressive stress regimes. In conjunction with the use of the response surface method, this study demonstrates the efficacy of the multilaminate framework and the Mohr–Coulomb constitutive model in providing a simplified, yet effective, probabilistic model of the mechanical behavior of caprock failure, reducing mathematical and computational complexities. [ABSTRACT FROM AUTHOR]

Taq DNA polymerases have played an important role in molecular biology for several years and are frequently used for polymerase chain reaction (PCR); hence, there is an increasing interest in developing a convenient method for preparing Taq DNA polymerase for routine use in laboratories. We developed a method using Escherichia coli (E. coli) that expresses thermostable Taq DNA polymerase directly in the PCR without purification. The Taq gene was transformed into E. coli and expressed. After overnight incubation and washing, E. coli-expressing Taq DNA polymerase (EcoliTaq) was used as the DNA polymerase without purification. EcoliTaq showed activity comparable to that of commercial DNA polymerase and remained stable for 3 months. With a high-pH buffer containing 2% Tween 20 and 0.4 M trehalose, EcoliTaq facilitated direct PCR amplification from anticoagulated whole blood samples. EcoliTaq exhibited good performance in allele-specific PCR using both purified DNA and whole blood samples. Furthermore, it proved to be useful as a DNA polymerase in hot-start PCR by effectively minimizing non-specific amplification. We developed a simple and cost-effective direct and hot-start PCR method in which EcoliTaq was used directly as a PCR enzyme, thus eliminating the laborious and time-consuming steps of polymerase purification. [ABSTRACT FROM AUTHOR]

This paper proposes the optimal algorithm for controlling the HVAC system in the target building. Previous studies have analyzed pre-selected algorithms without considering the unique data characteristics of the target building, such as location, climate conditions, and HVAC system type. To address this, we compare the accuracy of cooling load prediction using ANN and LSTM algorithms, widely used in building energy research, to determine the optimal algorithm for HVAC control in the target building. We develop a simulation model calibrated with actual data to ensure data reliability and compare the energy consumption of the existing HVAC control method and the two algorithms-based methods. Results show that the ANN algorithm, with a CV(RMSE) of 12.7%, has a higher prediction accuracy than the LSTM algorithm, CV(RMSE) of 17.3%, making it a more suitable algorithm for HVAC control. Furthermore, implementing the ANN-based approach results in a 3.2% cooling energy reduction from the optimal control of Air Handling Unit (AHU) Discharge Air Temperature (DAT) compared to the fixed DAT at 12.8 °C in a representative day. This study demonstrates that ML-based HVAC system control can effectively reduce cooling energy consumption in HVAC systems, providing an effective strategy for energy conservation and improved HVAC system efficiency. [ABSTRACT FROM AUTHOR]

A novel energy-saving hybrid cooling system that combines a forced-cooling cycle and a free-cooling cycle was developed to increase the energy efficiency of cooling systems in year-round operation of an internet data center with high heat loads. This system effectively utilizes a dam deep water source to reduce energy consumption in internet data centers. The hybrid cooling system operates in forced-cooling mode when the entering water temperature exceeds the mode change temperature of 9 °C, but switches to free-cooling mode when the ambient temperature falls below the mode change temperature. In this paper, the cooling performance of the hybrid system was assessed under various operating conditions based on entering water temperature fluctuation. Because the cooling effectiveness of this type of system is highly dependent on the outside climate, its usefulness and suitability for different periods and zones must be investigated. The annual energy saving performance of the new system was estimated and compared to a conventional cooling system in terms of the integrated coefficient of performance, based on the hourly weather air temperature and water temperature bin data collected from 16 cities of different climate zones in South Korea. The experimental findings revealed that the novel hybrid internet data center cooling system showed a 67% annual operating performance over a conventional air source internet data center cooling system due to the adoption of a dam deep water source. [ABSTRACT FROM AUTHOR]

The hyaluronic acid (HA) hydrogel array was employed for immobilization of 5-fluorouracil (5-FU), and the electrospun bilayer (hydrophilic: polyurethane/pluronic F-127 and hydrophobic: polyurethane) membrane was used to support the HA hydrogel array as a patch. To visualize the drug propagating phenomenon into tissues, we experimentally investigated how FITC-BSA diffused into the tissue by applying hydrogel patches to porcine tissue samples. The diffusive phenomenon basically depends on the FITC-BSA diffusion coefficient in the hydrogel, and the degree of diffusion of FITC-BSA may be affected by the concentration of HA hydrogel, which demonstrates that the high density of HA hydrogel inhibits the diffusive FITC-BSA migration toward the low concentration region. YD-10B cells were employed to investigate the release of 5-FU from the HA array on the bilayer membrane. In the control group, YD-10B cell viability was over 98% after 3 days. However, in the 5-FU-immobilized HA hydrogel array, most of the YD-10B cells were not attached to the bilayer membrane used as a scaffold. These results suggest that 5-FU was locally released and initiated the death of the YD-10B cells. Our results show that 5-FU immobilized on HA arrays significantly reduces YD-10B cell adhesion and proliferation, affecting cells even early in the cell culture. Our results suggest that when 5-FU is immobilized in the HA hydrogel array on the bilayer membrane as a drug patch, it is possible to control the drug concentration, to release it continuously, and that the patch can be applied locally to the targeted tumor site and administer the drug in a time-stable manner. Therefore, the developed bilayer membrane-based HA hydrogel array patch can be considered for sustained release of the drug in biomedical applications. [ABSTRACT FROM AUTHOR]

Buildings use up to 40% of the global primary energy and 30% of global greenhouse gas emissions, which may significantly impact climate change. Heating, ventilation, and air-conditioning (HVAC) systems are among the most significant contributors to global primary energy consumption and carbon gas emissions. Furthermore, HVAC energy demand is expected to rise in the future. Therefore, advancements in HVAC systems' performance and design would be critical for mitigating worldwide energy and environmental concerns. To make such advancements, energy modeling and model predictive control (MPC) play an imperative role in designing and operating HVAC systems effectively. Building energy simulations and analysis techniques effectively implement HVAC control schemes in the building system design and operation phases, and thus provide quantitative insights into the behaviors of the HVAC energy flow for architects and engineers. Extensive research and advanced HVAC modeling/control techniques have emerged to provide better solutions in response to the issues. This study reviews building energy modeling techniques and state-of-the-art updates of MPC in HVAC applications based on the most recent research articles (e.g., from MDPI's and Elsevier's databases). For the review process, the investigation of relevant keywords and context-based collected data is first carried out to overview their frequency and distribution comprehensively. Then, this review study narrows the topic selection and search scopes to focus on relevant research papers and extract relevant information and outcomes. Finally, a systematic review approach is adopted based on the collected review and research papers to overview the advancements in building system modeling and MPC technologies. This study reveals that advanced building energy modeling is crucial in implementing the MPC-based control and operation design to reduce building energy consumption and cost. This paper presents the details of major modeling techniques, including white-box, grey-box, and black-box modeling approaches. This paper also provides future insights into the advanced HVAC control and operation design for researchers in relevant research and practical fields. [ABSTRACT FROM AUTHOR]

The degeneration of an intervertebral disc (IVD) is a major cause of lower back pain. IVD degeneration is characterized by the abnormal expression of inflammatory cytokines and matrix degradation enzymes secreted by IVD cells. In addition, macrophage-mediated inflammation is strongly associated with IVD degeneration. However, the precise pathomechanisms of macrophage-mediated inflammation in IVD are still unknown. In this study, we developed a microfluidic platform integrated with an electrical stimulation (ES) array to investigate macrophage-mediated inflammation in human nucleus pulposus (NP). This platform provides multiple cocultures of different cell types with ES. We observed macrophage-mediated inflammation and considerable migration properties via upregulated expression of interleukin (IL)-6 (p < 0.001), IL-8 (p < 0.05), matrix metalloproteinase (MMP)-1 (p < 0.05), and MMP-3 (p < 0.05) in human NP cells cocultured with macrophages. We also confirmed the inhibitory effects of ES at 10 μA due to the production of IL-6 (p < 0.05) and IL-8 (p < 0.01) under these conditions. Our findings indicate that ES positively affects degenerative inflammation in diverse diseases. Accordingly, the microfluidic electroceutical platform can serve as a degenerative IVD inflammation in vitro model and provide a therapeutic strategy for electroceuticals. [ABSTRACT FROM AUTHOR]

The hybrid system of wave energy converters (WECs) using coastal structures is an attractive issue in terms of a decrease in construction costs and an improvement of the ability to capture wave energy. Most studies on the utilization of reflected waves from structures, which is one of the hybrid systems, are limited to mathematical analysis based on linear theories. Therefore, this paper presents fundamental experimental results in the presence of a reflection wall simplified as a coastal structure behind a bottom-hinged flap-type WEC under unidirectional regular waves. The behavioral characteristics and the power generation efficiency ke of the flap were investigated, focusing on wave steepness, initial water depth, and distance from the reflection wall. The results show that the condition of the initial water depth being smaller than the flap height is more effective in terms of avoiding unstable rotating of the flap. The maximum ke appeared slightly far from the node position of the standing waves because the flap shape and the power take-off (PTO) damping induce the phase difference between the reciprocating behavior of the flap and the period of the standing wave. The results imply that the optimum position of a WEC is dependent on WEC shape, PTO damping, and installation water depth. [ABSTRACT FROM AUTHOR]

Multicellular tumor spheroids (MCTs) have been employed in biomedical fields owing to their advantage in designing a three-dimensional (3D) solid tumor model. For controlling multicellular cancer spheroids, mimicking the tumor extracellular matrix (ECM) microenvironment is important to understand cell–cell and cell–matrix interactions. In drug cytotoxicity assessments, MCTs provide better mimicry of conventional solid tumors that can precisely represent anticancer drug candidates' effects. To generate incubate multicellular spheroids, researchers have developed several 3D multicellular spheroid culture technologies to establish a research background and a platform using tumor modelingvia advanced materials science, and biosensing techniques for drug-screening. In application, drug screening was performed in both invasive and non-invasive manners, according to their impact on the spheroids. Here, we review the trend of 3D spheroid culture technology and culture platforms, and their combination with various biosensing techniques for drug screening in the biomedical field. [ABSTRACT FROM AUTHOR]

Intervertebral disc (IVD) degeneration is a major cause of low back pain (LBP) in the lumbar spine. This phenomenon is caused by several processes, including matrix degradation in IVD tissues, which is mediated by matrix metalloproteinases (MMPs) and inflammatory responses, which can be mediated by interactions among immune cells, such as macrophages and IVD cells. In particular, interleukin (IL)-1 beta (β), which is a master regulator secreted by macrophages, mediates the inflammatory response in nucleus pulposus cells (NP) and plays a significant role in the development or progression of diseases. In this study, we developed a custom electrical stimulation (ES) platform that can apply low-constant-current stimulation (LCCS) signals to microfluidic chips. Using this platform, we examined the effects of LCCS on IL-1β-mediated inflammatory NP cells, administered at various currents (5, 10, 20, 50, and 100 μA at 200 Hz). Our results showed that the inflammatory response, induced by IL-1β in human NP cells, was successfully established. Furthermore, 5, 10, 20, and 100 μA LCCS positively modulated inflamed human NP cells' morphological phenotype and kinetic properties. LCCS could affect the treatment of degenerative diseases, revealing the applicability of the LCCS platform for basic research of electroceuticals. [ABSTRACT FROM AUTHOR]

In response to diverse pathogenic and danger signals, the cytosolic activation of the NLRP3 (NOD-, LRR-, and pyrin domain-containing (3)) inflammasome complex is a critical event in the maturation and release of some inflammatory cytokines in the state of an inflammatory response. After activation of the NLRP3 inflammasome, a series of cellular events occurs, including caspase 1-mediated proteolytic cleavage and maturation of the IL-1β and IL-18, followed by pyroptotic cell death. Therefore, the NLRP3 inflammasome has become a prime target for the resolution of many inflammatory disorders. Since NLRP3 inflammasome activation can be triggered by a wide range of stimuli and the activation process occurs in a complex, it is difficult to target the NLRP3 inflammasome. During the activation process, various post-translational modifications (PTM) of the NLRP3 protein are required to form a complex with other components. The regulation of ubiquitination and deubiquitination of NLRP3 has emerged as a potential therapeutic target for NLRP3 inflammasome-associated inflammatory disorders. In this review, we discuss the ubiquitination and deubiquitination system for NLRP3 inflammasome activation and the inhibitors that can be used as potential therapeutic agents to modulate the activation of the NLRP3 inflammasome. [ABSTRACT FROM AUTHOR]

Breaking waves generated by wave shoaling in coastal areas have a close relationship with various physical phenomena in coastal regions. Therefore, it is crucial to accurately predict breaker indexes such as breaking wave height and breaking depth when designing coastal structures. Many studies on wave breaking have been carried out, and many experimental data have been documented. Representative studies on wave breaking provide many empirical formulas for the prediction of breaking index, mainly through hydraulic model experiments. However, the existing empirical formulas for breaking index determine the coefficients of the assumed equation through statistical analysis of data under the assumption of a specific equation. This study presents an alternative method to estimate breaker index using representative linear-based supervised machine learning algorithms that show high predictive performance in various research fields related to regression or classification problems. Based on the used machine learning methods, a new simple linear equation for the prediction of breaker index is presented. The newly proposed breaker index formula showed similar predictive performance compared to the existing empirical formula, although it was a simple linear equation. [ABSTRACT FROM AUTHOR]

The detection and diagnosis of faults is becoming necessary in ensuring energy savings in heat pump units. Faults can exist independently or simultaneously in heat pumps at the refrigerant side and secondary fluid flow loops. In this work, we discuss the effects that simultaneous refrigerant charge faults and faults associated with the flow rate of secondary fluids have on the performance of a heat pump operating in summer season and we developed a correlation to detect and diagnose these faults using multiple linear regression. The faults considered include simultaneous refrigerant charge and indoor heat exchanger secondary fluid flow rate faults (IFRFs), simultaneous refrigerant charge and outdoor heat exchanger secondary fluid flow rate faults (OFRFs) and simultaneous refrigerant charge, IFRF and OFRF. The occurrence of simultaneous refrigerant charge fault, IFRF and OFRF caused up to a 5.7% and 8% decrease in cooling capacity compared to simultaneous refrigerant charge and indoor heat exchanger secondary fluid flow rate faults, and simultaneous refrigerant charge and outdoor heat exchanger secondary fluid flow rate faults, respectively. Simultaneous refrigerant charge fault, IFRF and OFRF resulted in up to an 11.6% and 5.9% decrease in COP of the heat pump unit compared to simultaneous refrigerant charge fault and IFRF, and simultaneous refrigerant charge fault and OFRF, respectively. The developed FDD correlations accurately predicted the simultaneous refrigerant charge and faults in the flow rate of the secondary fluid within an error margin of 7.7%. [ABSTRACT FROM AUTHOR]

Caspase-8 has been classified as an apoptotic caspase, and its initial definition was an initiator of extrinsic cell death. During the past decade, the concept of caspase-8 functioning has been changed by findings of its additional roles in diverse biological processes. Although caspase-8 was not originally thought to be involved in the inflammation process, many recent works have determined that caspase-8 plays an important role in the regulatory functions of inflammatory processes. In this review, we describe the recent advances in knowledge regarding the manner in which caspase-8 modulates the inflammatory responses concerning inflammasome activation, cell death, and cytokine induction. [ABSTRACT FROM AUTHOR]

Among the envelope components (e.g., walls, roofs, floors, and windows, etc.) affecting the cooling and heating load of buildings, windows are the most thermally vulnerable. Shading devices can minimize the thermal load on windows due to solar radiation and decrease radiation effects. However, the load changes due to convection and conduction should be considered. Therefore, when a shading device is applied to a window, control logic for thermal blocking and heat retention is necessary to prevent the load changes. In addition, by combining the opposite features of photovoltaic (PV) that require solar radiation and the shading device to block solar radiation, energy-saving and production can be achieved simultaneously. Therefore, this study minimized the thermal effects of windows using a movable shading device integrated with PV and evaluated the PV power generation. This study evaluated the effects on window heat transfer by applying artificial intelligence techniques, which have recently attracted attention, to system operation. To achieve this, artificial neural network (ANN)-based control logic was developed, and the control performance of the system was assessed using simulations. In ANN control, the window heat transfer was 86.3% lower in a cooling period and 9.7% lower in a heating period compared with that of a shading device fixed at 45°. Furthermore, the PV system produced 3.0 to 3.1% more electric power under optimal control during the cooling period. [ABSTRACT FROM AUTHOR]

Cornus officinalis, widely used in traditional Chinese medicine, exhibits pharmacological effects against erectile dysfunction and pollakisuria, which are pathological symptoms of benign prostatic hyperplasia (BPH). Although traditional usage and a study on BPH have been reported, to our knowledge, no study has investigated the exact molecular mechanism(s) underlying the anti-proliferative effects of standardized C. officinalis on prostatic cells. We standardized C. officinalis 30% ethanol extract (COFE) and demonstrated the therapeutic effects of COFE on human BPH epithelial cells and testosterone-induced BPH in rats. In vitro studies using BPH-1 cells demonstrated an upregulation of BPH-related and E2F Transcription Factor 1(E2F1)-dependent cell cycle markers, whereas treatment with COFE clearly inhibited the proliferation of BPH epithelial cells and reduced the overexpression of G1 and S checkpoint genes. Additionally, COFE administration alleviated the androgen-dependent prostatic enlargement in a testosterone-induced BPH animal model. COFE exerted these anti-BPH effects by the inhibition of anti-apoptotic markers, suppression of PCNA expression, and regulation of E2F1/pRB-dependent cell cycle markers in rats with BPH. These results suggest that COFE exerts anti-proliferative effect by regulating PCNA/E2F1-dependent cell cycle signaling pathway both in vivo and in vitro. These findings reveal the therapeutic potential of COFE, which could be used as a substitute for BPH treatment. [ABSTRACT FROM AUTHOR]

Rhubarb is a well-known herb worldwide and includes approximately 60 species of the Rheum genus. One of the representative plants is Rheum palmatum, which is prescribed as official rhubarb due to its pharmacological potential in the Korean and Chinese pharmacopoeia. In our bioactive screening, we found out that the EtOH extract of R. palmatum inhibited hepatic stellate cell (HSC) activation by transforming growth factor β1 (TGF-β1). Chemical investigation of the EtOH extract led to the isolation of chrysophanol 8-O-glucoside, which was determined by structural analysis using NMR spectroscopic techniques and electrospray ionization mass spectrometry (ESIMS). To elucidate the effects of chrysophanol 8-O-glucoside on HSC activation, activated LX-2 cells were treated for 48 h with chrysophanol 8-O-glucoside, and α-SMA and collagen, HSC activation markers, were measured by comparative quantitative real-time PCR (qPCR) and western blotting analysis. Chrysophanol 8-O-glucoside significantly inhibited the protein and mRNA expression of α-SMA and collagen compared with that in TGF-β1-treated LX-2 cells. Next, the expression of phosphorylated SMAD2 (p-SMAD2) and p-STAT3 was measured and the translocation of p-STAT3 to the nucleus was analyzed by western blotting analysis. The expression of p-SMAD2 and p-STAT3 showed that chrysophanol 8-O-glucoside strongly downregulated STAT3 phosphorylation by inhibiting the nuclear translocation of p-STAT3, which is an important mechanism in HSC activation. Moreover, chrysophanol 8-O-glucoside suppressed the expression of p-p38, not that of p-JNK or p-Erk, which can activate STAT3 phosphorylation and inhibit MMP2 expression, the downstream target of STAT3 signaling. These findings provided experimental evidence concerning the hepatoprotective effects of chrysophanol 8-O-glucoside against liver damage and revealed the molecular basis underlying its anti-fibrotic effects through the blocking of HSC activation. [ABSTRACT FROM AUTHOR]

In this study, the indoor illuminance of a full-scale mock-up with LED system and external automated blinds was quantitatively evaluated by performing experiments involving integrated blinds and LED dimming controls. Actual illuminance experimental data were constructed and analyzed using commercialized blind control logic. The results revealed changes in the LED dimming ratio (turning-off ratio), and that the indoor illuminance required for the indoor space of 500 ± 30 lx could be secured in all cases when daylight was introduced according to weather conditions. It was found that because the angle and vertical position (VP) were fixed, manual blind controls could not efficiently use daylight, even though they could cut off solar radiation. When the blinds were automatically controlled in three-steps based on the exterior vertical illuminance under clear sky, the dimming rates of Zones A and B were found to be 68 and 50%, respectively. When the blinds were automatically controlled in four-steps, the dimming rates of Zone A (1.5 m from the window) and Zone B (4.5 m from the window) were found to be at 70% and 52%, respectively. If the VP and slat angle could be adjusted without lowering all the blinds, it would be possible to secure work plane illuminance, because the inflow of daylight increases, without the direct impact of direct sunlight and glare. These results indicate that integrated blinds and LED dimming controls can contribute to maintaining standard illuminance and saving lighting energy using daylight. [ABSTRACT FROM AUTHOR]

The global incidence of breast cancer has increased. However, there are many impediments to the development of safe and effective anticancer drugs. The aim of the present study was to evaluate the effect of aviculin isolated from Lespedeza cuneata (Dum. Cours.) G. Don. (Fabaceae) on MCF-7 human breast cancer cells and determine the underlying mechanism. Using the bioassay-guided isolation by water soluble tetrazolium salt (WST-1)-based Ez-Cytox assay, nine compounds (four lignan glycosides (1–4), three flavonoid glycosides (5–7), and two phenolic compounds (8 and 9)) were isolated from the ethyl acetate (EA) fraction of the L. cuneata methanolic extract. Of these, aviculin (2), a lignan glycoside, was the only compound that reduced metabolic activity on MCF-7 cells below 50% (IC50: 75.47 ± 2.23 μM). The underlying mechanism was analyzed using the annexin V Alexa Fluor 488 binding assay and Western blotting. Aviculin (2) was found to induce apoptotic cell death through the intrinsic apoptosis pathway, as indicated by the increased expression of initiator caspase-9, executioner caspase-7, and poly (ADP-ribose) polymerase (PARP). Aviculin (2)-induced apoptotic cell death was accompanied by an increase in the Bax/Bcl-2 ratio. These findings demonstrated that aviculin (2) could induce breast cancer cell apoptosis through the intrinsic apoptosis pathway, and it can therefore be considered an excellent candidate for herbal treatment of breast cancer. [ABSTRACT FROM AUTHOR]

Concrete foundations have received attention as offshore wind turbine support structures because of their various advantages. However, because of the lack of information on structural analysis and the design method of complex marine environmental loads, concrete foundations cannot be applied on actual sites. Therefore, the structure behavior mechanism and concrete reinforcement design need to be evaluated based on soil-structure interactions. Herein, an efficient method for analysis of piled concrete foundations (PCFs) is presented, and the stability of PCF structures is evaluated under environmental conditions of the coast in Korea for a 3-MW wind turbine. Three analytical parameters for PCF models were defined to consider soil-structure interaction. The results of each model were compared with the displacement, stresses, and natural frequencies. Using the analysis results, a prestressing reinforcement design for concrete foundations was proposed. Quasi-static analysis showed that maximum displacement was sufficiently small and the maximum stresses did not exceed the allowable stresses. PCF showed excellent dynamic performance and structural stability. In addition, stiffness of the soil spring model influenced the natural frequency rather than the stiffness of the pile type. Detailed analysis of the connections between piles and concrete need to be studied in the future. [ABSTRACT FROM AUTHOR]

Hippophae rhamnoides L. (Elaeagnaceae; commonly known as "sea buckthorn" and "vitamin tree"), is a spiny deciduous shrub whose fruit is used in foods and traditional medicines. The H. rhamnoides fruit (berry) is rich in vitamin C, with a level exceeding that found in lemons and oranges. H. rhamnoides berries are usually washed and pressed to create pomace and juice. Today, the powder of the aqueous extract of H. rhamnoides berries are sold as a functional food in many countries. As part of our ongoing effort to identify bioactive constituents from natural resources, we aimed to isolate and identify those from the fruits of H. rhamnoides. Phytochemical analysis of the extract of H. rhamnoides fruits led to the isolation and identification of six compounds, namely, a citric acid derivative (1), a phenolic (2), flavonoids (3 and 4), and megastigmane compounds (5 and 6). Treatment with compounds 1–6 did not have any impact on the cell viability of RAW 264.7 mouse macrophages. However, pretreatment with these compounds suppressed lipopolysaccharide (LPS)-induced NO production in RAW 264.7 mouse macrophages in a concentration-dependent manner. Among the isolated compounds, compound 1 was identified as the most active, with an IC50 of 39.76 ± 0.16 μM. This value was comparable to that of the NG-methyl-L-arginine acetate salt, a nitric oxide synthase inhibitor with an IC50 of 28.48 ± 0.05 μM. Western blot analysis demonstrated that compound 1 inhibited the LPS-induced expression of IKKα/β (IκB kinase alpha/beta), I-κBα (inhibitor of kappa B alpha), nuclear factor kappa-B (NF-κB) p65, iNOS (inducible nitric oxide synthase), and COX-2 (cyclooxygenase-2) in RAW 264.7 cells. Furthermore, LPS-stimulated cytokine production was detected using a sandwich enzyme-linked immunosorbent assay. Compound 1 decreased interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) production in LPS-stimulated RAW 264.7 cells. In summary, the mechanism of action of 1 included the suppression of LPS-induced NO production in RAW 264.7 cells by inhibiting IKKα/β, I-κBα, NF-κB p65, iNOS, and COX-2, and the activities of IL-6 and TNF-α. [ABSTRACT FROM AUTHOR]

The purpose of this study is to investigate the effects of three external forces (tidal current, wind, and waves) on the movement of oil spilled during the Hebei Spirit oil spill accident. The diffusion of the spilled oil was simulated by using a random walk (RW) model that tracks the movement caused by advection-diffusion assuming oil as particles. For oil simulation, the wind drift current generated by wind and tidal current fields were computed by using the environmental fluid dynamics code (EFDC) model. Next, the wave fields were simulated by using the simulating waves nearshore (SWAN) model, and the Stokes drift current fields were calculated by applying the equation proposed by Stokes. The computed tidal currents, wind drift currents, and Stokes drift currents were applied as input data to the RW model. Then, oil diffusion distribution for each external force component was investigated and compared with that obtained from satellite images. When the wind drift currents and Stokes drift currents caused by waves were considered, the diffusion distribution of the spilled oil showed good agreement with that obtained from the observation. [ABSTRACT FROM AUTHOR]

The energy consumption for heating and cooling in the building sector accounts for more than one-third of total energy used worldwide. In view of that, it is important to develop energy efficient cooling and heating systems in order to conserve energy in buildings as well as reduce greenhouse gas emissions. In both commercial and residential buildings, the heat pump has been adopted as an energy efficient technology for space heating and cooling purposes as compared to conventional air conditioning systems. However, heat pumps undergo standard testing, rating, and certification procedures to ascertain their system performance. Essentially, the calorimeter for testing heat pumps has two test chambers to serve as a heat source and heat sink to control and maintain the test conditions required to simulate the heat pump indoor and outdoor units, simultaneously. In air-to-air heat pump units, the conventional calorimeter controls the air temperature and humidity conditions in each test chamber with separate air handling units consisting of a refrigerator, heater, humidifier, and supply fan, which results in high energy consumption. In this study, using dynamic modeling and simulation, a new calorimeter for controlling air conditions in each test chamber is proposed. The performance analysis based on simulation results showed that the newly proposed calorimeter predicted at least 43% energy savings with the use of a heat recovery unit and small refrigerator capacity as compared to the conventional calorimeter that utilized a large refrigerator capacity for all the weather conditions and load capacities that we investigated. [ABSTRACT FROM AUTHOR]

Garlic (Allium sativum L.) is utilized worldwide for culinary and medicinal use and has diverse health benefits. As part of our ongoing research to identify bioactive components from natural resources, phytochemical analysis of the methanolic extract of garlic led to the isolation and characterization of six compounds: Three eugenol diglycosides (1–3) and three β-carboline alkaloids (4–6). In particular, the absolute configurations of β-carboline alkaloids (5 and 6) were established by gauge-including atomic orbital nuclear magnetic resonance chemical shift calculations, followed by DP4+ analysis. Here, we evaluated the effects of compounds 1–6 on 3T3-L1 preadipocyte adipogenesis and lipid metabolism. 3T3-L1 adipocyte differentiation was evaluated using Oil Red O staining; the expression of adipogenic genes was detected using RT-qPCR. Among compounds 1–6, (1R,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (6) inhibited 3T3-L1 preadipocyte adipogenesis and reduced the expression of adipogenic genes (Fabp4, PPARγ, C/EBPβ, Adipsin, and Adipoq). Moreover, it markedly decreased the actylation of α-tubulin, which is crucial for cytoskeletal remodeling during adipogenesis. Anti-adipogenic effects were observed upon treatment with compound 6, not only during the entire process, but also on the first two days of adipogenesis. Additionally, treatment with compound 6 regulated the expression of genes involved in adipocyte lipid metabolism, decreasing the lipogenic gene (SREBP1) and increasing lipolytic genes (ATGL and HSL). We provide experimental evidence of the health benefits of using (1R,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid obtained from garlic to prevent excessive adipogenesis in obesity. [ABSTRACT FROM AUTHOR]

To provide useful information concerning energy-conserving heating, ventilation, and air-conditioning (HVAC) systems, this study used EnergyPlus to analyze in detail their operational characteristics and energy performance. This study also aimed to understand the features of the systems under consideration by investigating the dry-bulb temperature, relative humidity, and airflow rate at major nodes in each system's schematic. Furthermore, we analyzed the indoor environment created by each HVAC system, as well as examining the cooling energy consumptions and CO2 emissions. The HVAC systems selected for this study are the variable air volume (VAV) commonly used in office buildings (base-case model), constant air volume (CAV), under-floor air distribution (UFAD), and active chilled beam (ACB) with dedicated outdoor air system (DOAS). For the same indoor set-point temperature, the CAV's supply airflow was the highest, and VAV and UFAD were operated by varying the airflow rate according to the change of the space thermal load. ACB with DOAS was analyzed as being able to perform air conditioning only with the supply airflow constantly fixed at a minimum outdoor air volume. The primary cooling energy was increased by about 23.3% by applying CAV, compared to VAV. When using the UFAD and ACB with DOAS, cooling energy was reduced by 11.3% and 23.1% compared with VAV, respectively. [ABSTRACT FROM AUTHOR]

Programmed cell death pathways and inflammasome activation pathways can be genetically and functionally separated. Inflammasomes are specialized protein complexes that process pro-inflammatory cytokines, interleukin-1β (IL-1β), and IL-18 to bioactive forms for protection from a wide range of pathogens, as well as environmental and host-derived danger molecules. Programmed cell death has been extensively studied, and its role in the development, homeostasis, and control of infection and danger is widely appreciated. Apoptosis and the recently recognized necroptosis are the best-characterized forms of programmed death, and the interplay between them through death receptor signaling is also being studied. Moreover, growing evidence suggests that many of the signaling molecules known to regulate programmed cell death can also modulate inflammasome activation in a cell-intrinsic manner. Therefore, in this review, we will discuss the current knowledge concerning the role of the signaling molecules originally associated with programmed cell death in the activation of inflammasome and IL-1β processing. [ABSTRACT FROM AUTHOR]

Background: The aim of this study was to simplify and identify the contents of the herbal formula, HBX-5. This study was carried out to evaluate the therapeutic effects of HBX-6 in a mouse model of benign prostatic hyperplasia (BPH). Based on in vitro, we selected a candidate, reconstituted an experimental agent and investigated the effects on testosterone-induced BPH rats. Cell viability was determined by MTT assay in RWPE-1 and WPMY-1 cells. The expression of androgen receptor (AR) was measured in dihydrotestosterone-stimulated RWPE-1 and WPMY-1 cells. BPH was induced in mice by a subcutaneous injection of testosterone propionate for four weeks. Animals were divided into six groups: Group 1, control mice; Group 2, mice with BPH; Group 3, mice with BPH treated with finasteride; Group 4, mice with BPH treated with 200 mg/kg HBX-5; Group 5, mice with BPH treated with 100 mg/kg HBX-6; and Group 6, mice with BPH treated with 200 mg/kg HBX-6. Changes in prostate weight were measured after treatments, and the thickness of the epithelium was evaluated. The expression levels of proteins associated with prostatic cell proliferation and cell cycle-related proteins were determined. Based on previous reports and in vitro results, we selected Cornus officinalis and Psoralea corylifolia among HBX-5 components and reconstituted the experimental agent, and named it HBX-6. The result represented a new herbal formula, HBX-6 that suppressed the pathological alterations in BPH and showed a marked reduction in proliferation-related protein expression compared to mice with BPH. Our results indicate that HBX-6 has a better therapeutic effect in the BPH murine model than those of HBX-5 and finasteride, suggesting the role of HBX-6 as a new BPH remedial agent. [ABSTRACT FROM AUTHOR]

A ground source heat pump (GSHP) system has higher performance than air source heat pump system due to the use of more efficient ground heat source. However, the GSHP system performance depends on ground thermal properties and groundwater conditions. There are many studies on the improvement of GSHP system by developing ground heat exchanger (GHX) and heat exchange method. Several studies have suggested methods to improve heat exchange rate for the development of GHX. However, few real-scale experimental studies have quantitatively analyzed their performance using the same ground conditions. Therefore, the objective of this study was to evaluate the thermal performance of various pipe types of GHX by the thermal response test (TRT) under the same field and test conditions. Four kinds of GHX (HDPE type, HDPE-nano type, spiral fin type, and coaxial type) were constructed in the same site. Inlet and outlet temperatures of GHXs and effective thermal conductivity were measured through the TRT. In addition, the borehole thermal resistance was calculated to comparatively analyze the correlation of the heat exchange performance with each GHX. Result of the TRT revealed that averages effective thermal conductivities of HDPE type, HDPE-nano, spiral fin type, and coaxial type GHX were 2.25 W/m·K, 2.34 W/m·K, 2.55 W/m·K, and 2.16 W/m·K, respectively. In the result, it was found that the average borehole thermal resistance can be an important factor in TRT, but the effect of increased thermal conductivity of pipe material itself was not significant. [ABSTRACT FROM AUTHOR]

Refrigerant charge faults have a great adverse effect on the performance of heat pumps and must therefore be detected and diagnosed early in real time. In this study, the effect of refrigerant charge faults on a water-to-water heat pump is experimentally investigated in cooling mode and heating mode at various outdoor entering water temperature conditions. The study showed that refrigerant undercharge affects the performance of water-to-water heat pump more in heating mode than in cooling mode. Results from the study are used to develop a refrigerant charge fault detection and diagnosis (FDD) algorithm that works using correlations and rule-based refrigerant fault characteristic charts. The FDD algorithm is able to detect refrigerant charge faults in the water-to-water heat pump within an error threshold of ±4.5% and ±1.1% in cooling mode and heating mode respectively. [ABSTRACT FROM AUTHOR]

Necroptosis, or caspase-independent programmed cell death, is known to be involved in various pathological conditions, such as ischemia/reperfusion injury, myocardial infarction, atherosclerosis, and inflammatory bowel diseases. Although several inhibitors of necroptosis have been identified, none of them are currently in clinical use. In the present study, we identified a new compound, 4-({[5-(4-aminophenyl)-4-ethyl-4H-1,2,4-triazol-3-yl]sulfanyl}methyl)-N-(1,3-thiazol-2-yl) benzamide (NTB451), with significant inhibitory activity on the necroptosis induced by various triggers, such as tumor necrosis factor-α (TNF-α) and toll-like receptor (TLR) agonists. Mechanistic studies revealed that NTB451 inhibited phosphorylation and oligomerization of mixed lineage kinase domain like (MLKL), and this activity was linked to its inhibitory effect on the formation of the receptor interacting serine/threonine-protein kinase 1 (RIPK1)-RIPK3 complex. Small interfering RNA (siRNA)-mediated RIPK1 knockdown, drug affinity responsive target stability assay, and molecular dynamics (MD) simulation study illustrated that RIPK1 is a specific target of NTB451. Moreover, MD simulation showed a direct interaction of NTB451 and RIPK1. Further experiments to ensure that the inhibitory effect of NTB451 was restricted to necroptosis and NTB451 had no effect on nuclear factor-κB (NF-κB) activation or apoptotic cell death upon triggering with TNF-α were also performed. Considering the data obtained, our study confirmed the potential of NTB451 as a new necroptosis inhibitor, suggesting its therapeutic implications for pathological conditions induced by necroptotic cell death. [ABSTRACT FROM AUTHOR]

Benign prostatic hyperplasia (BPH), an age-dependent disorder with a prevalence percentage of 60% in the 60s, has been found to involve an androgenic hormone imbalance that causes confusion between cell apoptosis and proliferation. Because general medications for BPH treatment have undesirable side effects, the development of effective alternative medicines has been considered. HBX-5 is a newly developed formula with the aim of improving BPH, and is composed of nine medicinal herbs. BPH was induced in the rats by intramuscular injection of testosterone propionate after castration. Rats were divided into six groups, and the efficacy of HBX-5 on testosterone-induced BPH in rats was estimated. In addition, RWPE-1 and WPMY-1 cells were used to demonstrate the effect of HBX-5 on BPH in vitro model. Compared with the control group, HBX-5 administration group suppressed BPH manifestations, such as excessive development of prostate, and increase of serum dihydrotestosterone and 5α-reductase concentrations. Furthermore, immunohistochemistry analysis revealed that HBX-5 significantly decreased the expression of androgen receptor (AR) and proliferating cell nuclear antigen (PCNA). In addition, results of RWPE-1 and WPMY-1 cells showed that HBX-5 inhibited the over-expression of AR and PSA in DHT-induced prostate hyperplastic microenvironments. [ABSTRACT FROM AUTHOR]

This study aimed to develop a control algorithm that can operate a variable refrigerant flow (VRF) cooling system with optimal set-points for the system variables. An artificial neural network (ANN) model, which was designed to predict the cooling energy consumption for upcoming next control cycle, was embedded into the control algorithm. By comparing the predicted energy for the different set-point combinations of the control variables, the control algorithm can determine the most energy-effective set-points to optimally operate the cooling system. Two major processes were conducted in the development process. The first process was to develop the predictive control algorithm which embedded the ANN model. The second process involved performance tests of the control algorithm in terms of prediction accuracy and energy efficiency in computer simulation programs. The results revealed that the prediction accuracy between simulated and predicted outcomes proved to have a low coefficient of variation root mean square error (CVRMSE) value (10.30%). In addition, the predictive control algorithm markedly saved the cooling energy consumption by as much as 28.44%, compared to a conventional control strategy. These findings suggest that the ANN model and the control algorithm showed potential for the prediction accuracy and energy-effectiveness of VRF cooling systems. [ABSTRACT FROM AUTHOR]

To date, most of the indoor environment control is based on the dry-bulb air temperature, which is one of the simplified control methods having the limitation to truly represent the thermal comfort of individual occupants. A variety of factors affect the thermal comfort such as dry-bulb air temperature, humidity, air movement, radiation, clothing insulation, and metabolic activity level. In this circumstance, this study investigated the effects of considering hourly metabolic rate variations for predicted mean vote (PMV) control on the actual thermal load, energy usage, and life cycle cost (LCC). The case adopting PMV control taking the hourly metabolic rate into account was comparatively analyzed against the conventional dry-bulb air temperature control, using a detailed simulation technique after the validation process. As a result, when the activity state of the occupant is house cleaning in the summer, the indoor temperature decreases rapidly due to the high amount of activity. It requires a temperature that is 11.7 °C and 9.7 °C lower than the conventional dry-bulb air temperature control method, respectively, and generally forms a higher indoor air temperature than the conventional control method after 7 p.m. This means the difference in temperature to satisfy the comfort of the occupant according to the amount of activity, and during winter as opposed to summer, was found to form a lower indoor air temperature than the conventional temperature control. In case of annual boiler gas consumption, PMV control showed 7.3% less energy consumption than the dry-bulb air temperature control and showed 28.8% less energy consumption than the dry-bulb air temperature control for annual cooling electricity consumption. Considering the cooling and heating energy reduction rate and the initial installation cost of measuring equipment for real-time metabolic rate and PMV measurement, a payback period of approximately 4.15 years was required. [ABSTRACT FROM AUTHOR]

Emodin, an active constituent of oriental herbs, is widely used to treat allergy, inflammation, and other symptoms. This study provides the scientific basis for the anti-inflammasome effects of emodin on both in vitro and in vivo experimental models. Bone marrow-derived macrophages were used to study the effects of emodin on inflammasome activation by using inflammasome inducers such as ATP, nigericin, and silica crystals. The lipopolysaccharide (LPS)-induced endotoxin shock model was employed to study the effect of emodin on in vivo efficacy. Emodin treatment attenuated interleukin (IL)-1β secretion via the inhibition of NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome activation induced by ATP, nigericin, and silica crystals. Further, emodin ameliorated the severity of NLRP3 inflammasome-mediated symptoms in LPS-induced endotoxin mouse models. This study is the first to reveal mechanism-based evidence, especially with respect to regulation of inflammasome activation, substantiating traditional claims of emodin in the treatment of inflammation-related disorders.

Streptochlorin, a small compound derived from marine actinomycete, has been shown to have anti-angiogenic, anti-tumor, and anti-allergic activities. However, the anti-inflammatory effects and underlying mechanisms have not yet been reported. In the present study, we investigated the effect of streptochlorin on lipopolysaccharide (LPS)-induced inflammatory responses in vitro and in vivo. Streptochlorin attenuated the production of proinflammatory mediators such as nitric oxide, cyclooxygenase-2, pro-interleukin (IL)-1β, and IL-6 in LPS-stimulated RAW264.7 cells through inhibition of the Toll/interleukin-1 receptor (TIR)-domain-containing adapter-inducing interferon-β (TRIF)-dependent signaling pathway. Furthermore, streptochlorin suppressed the infiltration of immune cells such as neutrophils into the lung and proinflammatory cytokine production such as IL-6 and TNF-α in broncho-alveolar lavage fluid (BALF) in the LPS-induced acute lung injury (ALI) mouse model. Streptochlorin has potent anti-inflammatory effects through regulating TRIF-dependent signaling pathways, suggesting that streptochlorin may provide a valuable therapeutic strategy in treating various inflammatory diseases.

In an effort to develop short antimicrobial peptides with simple amino acid compositions, we generated a series of undecapeptide isomers having the L(5)K(5)W formula. Amino acid sequences were designed to be perfectly amphipathic when folded into a helical conformation by converging leucines onto one side and lysines onto the other side of the helical axis. The single tryptophans, whose positions were varied in the primary structures, were located commonly at the critical amphipathic interface in the helical wheel projection. Helical conformations and the tryptophanyl environments of the 11 L(5)K(5)W peptides were confirmed and characterized by circular dichroism, fluorescence and nuclear magnetic resonance spectroscopy. All of the isomers exhibited a potent, broad-spectrum of antibacterial activity with just a slight variance in individual potency, whereas their hemolytic activities against human erythrocytes were significantly diversified. Interestingly, helical dispositions and fluorescence blue shifts of the peptides in aqueous trifluoroethanol solutions, rather than in detergent micelles, showed a marked linear correlation with their hemolytic potency. These results demonstrate that our de novo design strategy for amphipathic helical model peptides is effective for developing novel antimicrobial peptides and their hemolytic activities can be estimated in correlation with structural parameters.