1. Six Decades of Research on Human Fetal Gonadal Steroids
- Author
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Connan-Perrot, Stéphane, Léger, Thibaut, Lelandais, Pauline, Desdoits-Lethimonier, Christele, David, Arthur, Fowler, Paul, Mazaud-Guittot, Séverine, Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Laboratoire de Fougères - ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), École des Hautes Études en Santé Publique [EHESP] (EHESP), Laboratoire d'étude et de recherche en environnement et santé (LERES), Département Santé Environnement Travail et Génie Sanitaire (DSETGS), University of Aberdeen, the Marie Skłodowska-Curie PROTECTED projects (grant agreement No. 722634), Anses, European Project: 825100,FREIA, Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Chiffoleau, Emmanuelle, and Female Reproductive toxicity of EDCs: a human evidence-based screening and Identification Approach - FREIA - 825100 - INCOMING
- Subjects
Male ,déveoppement fétal ,steroidogenesis ,analysis ,QH301-705.5 ,hormone ,detection ,Review ,testis ,Mass Spectrometry ,gonade ,Fetus ,Humans ,human ,Biology (General) ,Gonadal Steroid Hormones ,Gonads ,QD1-999 ,[SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis ,[SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism ,perturbateur endocrinien ,Immunoassay ,[SDV.BDD.GAM] Life Sciences [q-bio]/Development Biology/Gametogenesis ,Research ,Sexual Development ,Ovary ,toxicologie ,androgens ,[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism ,fetal ,détection ,quantification ,[SDV.TOX] Life Sciences [q-bio]/Toxicology ,Chemistry ,[SDV.TOX]Life Sciences [q-bio]/Toxicology ,stéroidogenèse ,Female ,contaminant ,estrogens - Abstract
International audience; Human fetal gonads acquire endocrine steroidogenic capabilities early during their differentiation. Genetic studies show that this endocrine function plays a central role in the sexually dimorphic development of the external genitalia during fetal development. When this endocrine function is dysregulated, congenital malformations and pathologies are the result. In this review, we explain how the current knowledge of steroidogenesis in human fetal gonads has benefited from both the technological advances in steroid measurements and the assembly of detailed knowledge of steroidogenesis machinery and its expression in human fetal gonads. We summarise how the conversion of radiolabelled steroid precursors, antibody-based assays, mass spectrometry, ultrastructural studies, and the in situ labelling of proteins and mRNA have all provided complementary information. In this review, our discussion goes beyond the debate on recommendations concerning the best choice between the different available technologies, and their degrees of reproducibility and sensitivity. The available technologies and techniques can be used for different purposes and, as long as all quality controls are rigorously employed, the question is how to maximise the generation of robust, reproducible data on steroid hormones and their crucial roles in human fetal development and subsequent functions.
- Published
- 2021