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Research on systems that imitate the gaze function of human eyes is valuable for the development of humanoid eye intelligent perception. However, the existing systems have some limitations, including the redundancy of servo motors, a lack of camera position adjustment components, and the absence of interest-point-driven binocular cooperative motion-control strategies. In response to these challenges, a novel biomimetic binocular cooperative perception system (BBCPS) was designed and its control was realized. Inspired by the gaze mechanism of human eyes, we designed a simple and flexible biomimetic binocular cooperative perception device (BBCPD). Based on a dynamic analysis, the BBCPD was assembled according to the principle of symmetrical distribution around the center. This enhances braking performance and reduces operating energy consumption, as evidenced by the simulation results. Moreover, we crafted an initial position calibration technique that allows for the calibration and adjustment of the camera pose and servo motor zero-position, to ensure that the state of the BBCPD matches the subsequent control method. Following this, a control method for the BBCPS was developed, combining interest point detection with a motion-control strategy. Specifically, we propose a binocular interest-point extraction method based on frequency-tuned and template-matching algorithms for perceiving interest points. To move an interest point to a principal point, we present a binocular cooperative motion-control strategy. The rotation angles of servo motors were calculated based on the pixel difference between the principal point and the interest point, and PID-controlled servo motors were driven in parallel. Finally, real experiments validated the control performance of the BBCPS, demonstrating that the gaze error was less than three pixels. [ABSTRACT FROM AUTHOR]

Simple Summary: High density (HD) rearing seriously hinders the healthy development of broilers. Chlorogenic acid, a natural antioxidant food and drug source, may relieve the HD oxidative stress. Therefore, the present study was to investigate the effects of CGA supplementation on growth performance, antioxidant index in serum, relative mRNA expression of jejunum, and cecum microflora of broilers expose to HD stress. A total of 476 7-day-old AA+ broilers were divided into normal density (ND group, 14 broilers/m2) and high density (HD group, 22 broilers/m2), and CGA supplementation (ND or HD + CGA) group. The results showed that HD stress decreased the serum antioxidant capacity, inhibited the development of intestinal villi and the expression of tight junction genes, and thus affected the intestinal permeability during the rapid growth period from 21 to 35 days. Meanwhile, with the progression of HD oxidative stress, adverse changes occurred in intestinal microorganisms, leading to intestinal inflammation, and finally affected the growth performance on day 42. Additionally, dietary CGA can inhibit the oxidative stress from serum to intestinal tract, improve intestinal integrity, and enhance beneficial flora of HD broilers. Thus, dietary supplementations of an antioxidant such as CGA can effectively prevent oxidative stress in HD broilers. Chlorogenic acids (CGA) are widely used as feed additives for their ability to improve growth performance and intestinal health in poultry. However, whether dietary CGAs could reverse the impaired intestinal condition caused by high stocking density (HD) in broiler chickens is unknown. We determined the effect of dietary CGA on growth, serum antioxidant levels, jejunum barrier function, and the microbial community in the cecum of broilers raised under normal (ND) or HD conditions. HD stress significantly decreased growth and body weight, which was restored by CGA. The HD group showed increased serum malondialdehyde, an oxidative byproduct, and decreased SOD and GSH-Px activity. CGA reduced malondialdehyde and restored antioxidant enzyme activity. HD stress also significantly decreased jejunal villus length and increased crypt depth. Compared with ND, the expression of tight-junction genes was significantly decreased in the HD group, but this decrease was reversed by CGA. HD also significantly upregulated TNF-α. Compared with ND, the cecal microbiota in the HD group showed lower alpha diversity with increases in the harmful bacteria Turicibacter and Shigella. This change was altered in the HD + CGA group, with enrichment of Blautia, Akkermansia, and other beneficial bacteria. These results demonstrated that HD stress decreased serum antioxidant capacity, inhibited the development of jejunal villi, and downregulated expression of tight-junction genes, which increased intestinal permeability during the rapid growth period (21 to 35 days). Dietary CGA enhanced antioxidant capacity, improved intestinal integrity, and enhanced beneficial gut bacteria in chickens raised under HD conditions. [ABSTRACT FROM AUTHOR]

Simple Summary: Immune stress and high stocking density stress are two major concerns in poultry production, as they can greatly impair the productive and reproductive performance of chickens with serious economic consequences. Chlorogenic acid has been widely used as a feed additive in poultry production due to its potent antioxidant, anti-inflammatory, antibacterial, and antiviral activities. This study aimed to investigate the effects of dietary chlorogenic acid supplementation on growth performance, antioxidant function, and immune response in broiler breeders exposed to immune stress and high stocking density stress. Our study highlights that chlorogenic acid increased feed intake, downregulated serum corticosterone levels, and altered the immune and antioxidant functions of broiler breeders exposed to immune stress or high stocking density stress. Dietary inclusion of 1 g/kg chlorogenic acid could be used to increase feed intake of broiler breeders and alleviate the effects of immune stress and exposure to high stocking density in poultry. The study was conducted to evaluate the effects of dietary chlorogenic acid supplementation on the growth performance, antioxidant function, and immune response of broiler breeders exposed to immune stress or high stocking density stress. The test was divided into two stress models. For the immune stress test, 198 birds were distributed into three experimental treatments with six replicates per treatment. The treatments were: (1) saline control (birds injected with saline and fed basal diet), (2) LPS group (birds injected with 0.5 mg LPS/kg body weight and fed basal diet), and (3) CGA + LPS group (birds injected with LPS and fed basal diet supplemented with 1 g/kg CGA. LPS was intraperitoneally injected from day 14, and then daily for 10 days. For the high stocking density stress model, 174 birds were distributed into three experimental treatments with six replicates per treatment. The treatments were: (1) controls (birds fed basal diet and raised at a stocking density of 14 broilers per m2), (2) high-density group (birds fed with basal diet and raised at a stocking density of 22 broilers per m2), and (3) high density + CGA group (birds fed with 1 g/kg CGA and raised at a stocking density of 22 broilers per m2). Results showed that LPS injection and high stocking density significantly decreased the body weight and feed intake of broiler breeders, while CGA supplementation increased feed intake of broiler breeders under LPS injection and high stocking density stress. Moreover, LPS injection and high stocking density increased the concentration of corticosterone in serum, and CGA addition remarkably downregulated serum corticosterone levels. The GSH level decreased with LPS injection and CGA increased the GSH concentration in the intestines of immune-stressed broiler breeders. LPS injection promoted the production of circulating proinflammatory cytokines (serum IL-1β and TNF-α) by 72 h after LPS injection. Dietary supplementation with CGA prevented the increase in serum TNF-α caused by LPS. These results suggest that dietary inclusion of 1 g/kg CGA could increase the feed intake of broiler breeders and alleviate the effects of inflammatory mediator stress and exposure to high stocking density. [ABSTRACT FROM AUTHOR]