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Human mitochondria contain a circular genome that encodes 13 subunits of the oxidative phosphorylation system. In addition to their role as powerhouses of the cells, mitochondria are also involved in innate immunity as the mitochondrial genome generates long double-stranded RNAs (dsRNAs) that can activate the dsRNA-sensing pattern recognition receptors. Recent evidence shows that these mitochondrial dsRNAs (mt-dsRNAs) are closely associated with the pathogenesis of human diseases that accompany inflammation and aberrant immune activation, such as Huntington's disease, osteoarthritis, and autoimmune Sjögren's syndrome. Yet, small chemicals that can protect cells from a mt-dsRNA-mediated immune response remain largely unexplored. Here, we investigate the potential of resveratrol (RES), a plant-derived polyphenol with antioxidant properties, on suppressing mt-dsRNA-mediated immune activation. We show that RES can revert the downstream response to immunogenic stressors that elevate mitochondrial RNA expressions, such as stimulation by exogenous dsRNAs or inhibition of ATP synthase. Through high-throughput sequencing, we find that RES can regulate mt-dsRNA expression, interferon response, and other cellular responses induced by these stressors. Notably, RES treatment fails to counter the effect of an endoplasmic reticulum stressor that does not affect the expression of mitochondrial RNAs. Overall, our study demonstrates the potential usage of RES to alleviate the mt-dsRNA-mediated immunogenic stress response. [ABSTRACT FROM AUTHOR]

Assessing the biomass of zooplankton compensates for the difference between number of individuals and the accumulated body weight of the community, which helps assess aquatic ecosystem food web functions. Daphnia are crustaceans that play an intermediate role in biological interactions within food webs. The morphology and body specification of Daphnia differ during growth; hence, it is essential to apply species-specific equations to estimate biomass. We evaluated the length–weight regression equations used previously to estimate Daphnia magna biomass and conducted regression analyses using various body specifications and biomass measurements taken directly using devices such as a microbalance and microscopic camera. Biomass estimated using an equation from the Environmental Protection Agency was significantly different from the direct measurement: average biomass was lower, indicating that the equation possibly underestimated actual biomass. The biomass of D. magna had a higher multiple R2 value when length was compared with width and area, and a linear regression equation was the most suitable equation for biomass estimation. Because body specifications and biomass are affected by various environmental factors, the development of accurate species-specific biomass estimation equations will contribute to obtaining fundamental data with which the biological responses of zooplankton to aquatic ecosystem changes can be assessed. [ABSTRACT FROM AUTHOR]