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DNA-mimicking proteins encoded by viruses can modulate processes such as innate cellular immunity. An example is Ung-family uracil-DNA glycosylase inhibition, which prevents Ung-mediated degradation via the stoichiometric protein blockade of the Ung DNA-binding cleft. This is significant where uracil-DNA is a key determinant in the replication and distribution of virus genomes. Unrelated protein folds support a common physicochemical spatial strategy for Ung inhibition, characterised by pronounced sequence plasticity within the diverse fold families. That, and the fact that relatively few template sequences are biochemically verified to encode Ung inhibitor proteins, presents a barrier to the straightforward identification of Ung inhibitors in genomic sequences. In this study, distant homologs of known Ung inhibitors were characterised via structural biology and structure prediction methods. A recombinant cellular survival assay and in vitro biochemical assay were used to screen distant variants and mutants to further explore tolerated sequence plasticity in motifs supporting Ung inhibition. The resulting validated sequence repertoire defines an expanded set of heuristic sequence and biophysical signatures shared by known Ung inhibitor proteins. A computational search of genome database sequences and the results of recombinant tests of selected output sequences obtained are presented here. [ABSTRACT FROM AUTHOR]

Proton-conducting solid–oxide fuel cell (H-SOFC) is an alternative promising low-temperature electrochemical cell for renewable energy, but the performance is insufficient because of the low activity of cathode materials at low temperatures. A layered perovskite oxide PrBaFe1.9Zn0.1O5+δ (PBFZ) was synthesized and investigated as a promising cathode material for low-temperature H-SOFC. Here, the partial substitution of Fe by Zn further enhances the electrical conductivity and thermal compatibility of PrBaFe2O5+δ (PBF). The PBFZ exhibits improved conductivity in the air at intermediate temperatures and good chemical compatibility with electrolytes. The oxygen vacancy formed at the PBFZ lattice due to Zn doping enhances proton defects, resulting in an improved performance by extending the catalytic sites to the whole cathode area. A single cell with a Ni-BZCY anode, PBFZ cathode, and BaZr0.7Ce0.2Y0.1O3-δ (BZCY) electrolyte membrane was successfully fabricated and tested at 550–700 °C. The maximum power density and Rp were enhanced to 513 mW·cm−2 and 0.3 Ω·cm2 at 700 °C, respectively, due to Zn doping. [ABSTRACT FROM AUTHOR]

The electron beam welding process is widely used in the connection among titanium alloy material parts of aero-engines. Its mechanical properties need to meet the requirements of long life and high reliability. In this paper, the static strength and the fatigue failure behavior of the electron beam weldments of TC17 titanium alloy were investigated experimentally under low amplitude high frequency (20 kHz), and the mechanical response and failure mechanism under different external loading conditions were analyzed. In summary, the samples were found to have anisotropic microstructure. The tensile strength of the PWHT of TC17 EBW joint was ~4.5% lower than that of the base metal. Meanwhile, compared with the base metal, the fatigue strength was reduced by 45.5% at 109 cycles of fatigue life. The fracture analysis showed that the fatigue failure of the welded joint of TC17 alloy was caused by the welded pores and the fatigue cracks initiated from the welded pores. A fine granular area (FGA) was observed around the crack initiation region. The existence of pores caused the stress intensity factor of the fine granular area (KFGA) to be inversely proportional to the fatigue life. The KFGA calculation formula was modified and the fatigue crack propagation threshold of the welded joint of TC17 alloy was calculated (3.62 MPa·m1/2). Moreover, the influences of the effective size and the relative depth of the pores on the very long fatigue life of the electron beam welded joint of TC17 titanium alloy were discussed. [ABSTRACT FROM AUTHOR]