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In practical applications such as cancer diagnosis and industrial detection, there is a critical demand for fast fluorescence lifetime imaging (Fast-FLIM). The Fast-FLIM systems suitable for complex environments are typically achieved by enhancing the hardware performance of time-correlated single-photon counting (TCSPC), with an acquisition speed of about a few frames per second (fps). However, due to the limitation of single-photon acquisition, the imaging speed is still far from the demand of practical application. The synchroscan streak camera (SC) maps signals from the temporal dimension to the spatial dimension, effectively overcoming the long acquisition time caused by single-photon acquisition. This paper constructs a method to calculate the acquisition time for the TCSPC-FLIM and SC-FLIM systems, and it quantitatively compares the speed. The research demonstrates that the main factors limiting the acquisition speed of the FLIM systems are the photon emission rate, the photon counting rate, the required SNR, the dwell time, and the number of parallel channels. In high-quality and large-scale lifetime imaging, the acquisition speed of the SC-FLIM is at least 10 4 times faster than that of the TCSPC-FLIM. Therefore, the synchroscan streak camera has more significant potential to promote Fast-FLIM. [ABSTRACT FROM AUTHOR]

The implementation of renewable portfolio standards (RPS) and tradable green certificate schemes will exert significant influences on the market equilibrium outcomes and generation firms' strategic behaviors. To quantitatively investigate these influences, firstly, considering the difference in power generation cost and the uncertainty of renewable energy power generation, the equilibrium model for various trade subjects in the electricity market is established. Secondly, the nondominated sorting genetic algorithm II is adopted for solving the equilibrium model to find well-distributed Pareto-optimal solutions. Finally, the grey relational projection method is used to calculate the priority membership of each decision-making scheme so as to determine the optimal compromise solution. The simulation focuses on analyzing the impact of RPS on the equilibrium results and market behavior of power generators and introduces the Lerner index to quantify the market power of generators. The results show that: (1) An increase in the quota ratio can effectively increase power generation in renewable energy generators. The game between thermal power generators and renewable energy generators raises the prices of both markets at the same time. (2) Improving the forecasting accuracy is conducive to alleviating the market power behavior of various power generators, thereby ensuring the healthy operation of the power market. [ABSTRACT FROM AUTHOR]

Featured Application: Nanosensors for extracellular oxygen-sensing. Three four-arm amphiphilic block copolymers with different chain lengths, consisting of a hydrophilic chain of polyethylene glycol (PEG) and hydrophobic segment of polycaprolactam (PCL), were synthesized and used to encapsulate the high-efficient and hydrophobic oxygen probe of platinum(II)-5,10,15,20-tetrakis-(2,3,4,5,6-pentafluorophenyl)-porphyrin (PtTFPP) to form polymer micelles. This approach enabled the use of PtTFPP in aqueous solution for biosensing. Experimental results demonstrated that the particle sizes of these nano-oxygen sensors between 40.0 and 203.8 nm depend on the structures of block copolymers. PtTFPP in these micelles showed an effective quantum yield under nitrogen environment, ranging from 0.06 to 0.159. The new sensors are suitable for analyzing dissolved oxygen concentrations in the range of 0.04–39.3 mg/L by using the linear Stern–Volmer equation at room temperature. In addition, it has been shown that these sensors are capable of in situ monitoring the dissolved oxygens in the culture medium of E. coli and Romas cells during the respiration process, and distinguishing the drug activity of antibiotic ampicillin from that of antimycin A. This study showed that the use of these nanostructured multi-arm block copolymer micelles can achieve efficient biological applications without specific structural modification of the hydrophobic PtTFPP probe, which is expected to have broad prospects. [ABSTRACT FROM AUTHOR]

Due to the uncertainty of wind power output, the congestion of wind power has become prominent. Exactly how to improve the capacity of wind power consumption has become a problem that needs to be studied urgently. In this paper, an energy storage system and energy-extensive load with adjustable characteristics are used as an important means of consuming wind power. Firstly, we analyze the reasons for the congestion according to the characteristics of wind power output, and establish a model of the grid's ability to integrate wind power based on the concept of a wind power admissible interval. Secondly, we analyze the energy-extensive load regulation characteristics and establish an energy-extensive load dispatch model. Thirdly, on the basis of considering the energy-extensive load and energy storage system adjustment constraints, a bi-level optimization model is established. The upper level determines the configured capacity of the energy storage system with the goal of minimizing the total economic investment of the energy storage system, and the lower level coordinates the dispatching with the goal of maximizing wind power consumption and minimizing system operating costs. Finally, a certain region is taken as an example to verify the validity of the proposed method. [ABSTRACT FROM AUTHOR]

New amphiphilic star or multi-arm block copolymers with different structures were synthesized for enabling the use of hydrophobic oxygen probe of platinum (II)-tetrakis (pentafluorophenyl) porphyrin (PtTFPP) for bioanalysis. The amphiphilic star polymers were prepared through the Atom Transfer Radical Polymerization (ATRP) method by using hydrophilic 4-arm polyethylene glycol (4-arm-PEG) as an initiator. Among the five block copolymers, P1 series (P1a, P1b, and P1c) and P3 possess fluorine-containing moieties to improve the oxygen sensitivity with its excellent capacity to dissolve and carry oxygen. A polymer P2 without fluorine units was also synthesized for comparison. The structure-property relationship was investigated. Under nitrogen atmosphere, high quantum efficiency of PtTFPP in fluorine-containing micelles could reach to 22% and long lifetime could reach to 76 μs. One kind of representative PtTFPP-containing micelles was used to detect the respiration of Escherichia coli (E. coli) JM109 and macrophage cell J774A.1 by a high throughput plate reader. In vivo hypoxic imaging of tumor-bearing mice was also achieved successfully. This study demonstrated that using well-designed fluoropolymers to load PtTFPP could achieve high oxygen sensing properties, and long lifetime, showing the great capability for further in vivo sensing and imaging. [ABSTRACT FROM AUTHOR]