1. Identification of Haplotype Tag Single-Nucleotide Polymorphisms within the PPAR Family Genes and Their Clinical Relevance in Patients with Major Trauma
- Author
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Ling Zeng, Lian-Yang Zhang, Wei Gu, Xiao Wang, Jianxin Jiang, Jun-wei Gao, Anqiang Zhang, Wei Pan, and Ding-Yuan Du
- Subjects
Lipopolysaccharides ,Male ,Health, Toxicology and Mutagenesis ,Peroxisome Proliferator-Activated Receptors ,Peroxisome proliferator-activated receptor ,lcsh:Medicine ,Bioinformatics ,MODS ,sepsis ,0302 clinical medicine ,Genotype ,Leukocytes ,genetic polymorphism ,peroxisome proliferator-activated receptor ,trauma ,chemistry.chemical_classification ,Middle Aged ,030220 oncology & carcinogenesis ,Female ,medicine.symptom ,Adult ,Adolescent ,Multiple Organ Failure ,Single-nucleotide polymorphism ,Polymorphism, Single Nucleotide ,Article ,Sepsis ,03 medical and health sciences ,Young Adult ,Asian People ,medicine ,Humans ,Allele ,Alleles ,Aged ,business.industry ,Tumor Necrosis Factor-alpha ,Haplotype ,Organ dysfunction ,lcsh:R ,Public Health, Environmental and Occupational Health ,030208 emergency & critical care medicine ,medicine.disease ,chemistry ,Haplotypes ,Immunology ,Wounds and Injuries ,business ,Multiple organ dysfunction syndrome - Abstract
Background: Peroxisome proliferator-activated receptors (PPARs) play important roles in the development of inflammatory diseases and sepsis. Recently, genetic variants of PPARs genes have been widely studied in some inflammatory diseases. However, the association between PPAR family of genes polymorphisms and sepsis risk in trauma patients was little known. Methods: SNPs were selected from the PPARs genes through constructing haplotype blocks and genotyped by the improved multiplex ligation detection reaction (iMLDR) method. The association between the selected SNPs and the risk of sepsis and multiple organ dysfunction (MOD) scores was evaluated in 734 trauma patients. In addition, tumor necrosis factor α (TNFα) production of peripheral blood leukocytes was also analyzed after lipopolysaccharide (LPS) stimulation. Results: Our results revealed that there were significant associations between the rs10865710 polymorphism and the risk of sepsis and MOD scores in Chinese Han trauma patients. Further, we found that the level of TNFα production was higher in patients with the rs10865710 G allele compared to those with the variant C allele. Conclusions: The rs10865710 polymorphism in the PPARγ gene might be used to assess the risk of sepsis and multiple organ dysfunction syndrome (MODS) in trauma patients.
- Published
- 2016