1. Development of a Topical Insulin Polymeric Nanoformulation for Skin Burn Regeneration: An Experimental Approach
- Author
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Catarina Pinto Reis, Ana S. Viana, Lia Ascensão, Maria Quitério, Ana Paula Leandro, Sandra Simões, Maria Manuela Gaspar, Isabel Correia, Pedro Faísca, Andreia Ascenso, Manuela Carvalheiro, and Jesús Molpeceres
- Subjects
Time Factors ,Administration, Topical ,medicine.medical_treatment ,wound healing ,02 engineering and technology ,lcsh:Chemistry ,Mice ,chemistry.chemical_compound ,0302 clinical medicine ,Polylactic Acid-Polyglycolic Acid Copolymer ,HaCaT Cells ,lcsh:QH301-705.5 ,Spectroscopy ,Skin ,Protein Stability ,Chemistry ,Circular Dichroism ,PLGA ,General Medicine ,topical administration ,021001 nanoscience & nanotechnology ,Controlled release ,Computer Science Applications ,030220 oncology & carcinogenesis ,Female ,Burns ,0210 nano-technology ,insulin ,Cell Survival ,Drug Compounding ,Static Electricity ,Catalysis ,Article ,Inorganic Chemistry ,03 medical and health sciences ,In vivo ,medicine ,Animals ,Humans ,Regeneration ,Viability assay ,Particle Size ,Physical and Theoretical Chemistry ,Molecular Biology ,Insulin ,Regeneration (biology) ,Organic Chemistry ,Drug Liberation ,lcsh:Biology (General) ,lcsh:QD1-999 ,Biophysics ,nanoparticles ,Nanocarriers ,Wound healing - Abstract
Insulin is a peptide hormone with many physiological functions, besides its use in diabetes treatment. An important role of insulin is related to the wound healing process—however, insulin itself is too sensitive to the external environment requiring the protective of a nanocarrier. Polymer-based nanoparticles can protect, deliver, and retain the protein in the target area. This study aims to produce and characterize a topical treatment for wound healing consisting of insulin-loaded poly-DL-lactide/glycolide (PLGA) nanoparticles. Insulin-loaded nanoparticles present a mean size of approximately 500 nm and neutral surface charge. Spherical shaped nanoparticles are observed by scanning electron microscopy and confirmed by atomic force microscopy. SDS-PAGE and circular dichroism analysis demonstrated that insulin preserved its integrity and secondary structure after the encapsulation process. In vitro release studies suggested a controlled release profile. Safety of the formulation was confirmed using cell lines, and cell viability was concentration and time-dependent. Preliminary safety in vivo assays also revealed promising results.
- Published
- 2021