1. Evaluating the Efficacy of Plant Extracts in Managing the Bruchid Beetle, Callosobruchus maculatus (Coleoptera: Bruchidae).
- Author
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Akbar, Rasheed, Faheem, Brekhna, Aziz, Tariq, Ali, Amjad, Ullah, Asmat, Khan, Imtiaz Ali, and Sun, Jianfan
- Subjects
COWPEA weevil ,PROBIT analysis ,PLANT extracts ,INTEGRATED pest control ,PHYTOCHEMICALS ,TOBACCO - Abstract
Simple Summary: This research reveals the efficacy of five plant extracts (Nicotiana tabacum, Nicotiana rustica, Azadirachta indica, Thuja orientalis, and Melia azedarach) against Callosobruchus maculatus. The extracts were tested at six different concentrations with four replications. Phytochemical analysis revealed varying amounts of alkaloids, flavonoids, saponins, diterpenes, phytosterols, and phenols. Among the plants, A. indica showed the highest effectiveness, with the lowest infestation rate (16.65%), seed weight loss (7.85%), mean oviposition (84.54), and adult emergence (58.40%). Conversely, T. orientalis was the least effective. Toxicity analysis using probit analysis indicated that N. tabacum, N. rustica, and A. indica had the highest toxicity, as demonstrated by the lowest LC
50 and LC90 values. Repellency tests showed that A. indica exhibited 100.00% highest repellency at a 3% concentration after 48 h against C. maculatus. This study suggests incorporating these plant extracts, especially N. rustica, N. tabacum, A. indica, and M. azedarach, into integrated pest management programs, to manage C. maculatus. An estimated 2000 plant species have been employed for pest control worldwide. The use of these botanical derivatives is thought to be one of the most cost-effective and sustainable options for pest management in stored grain. The present study was designed to assess the efficacy of five plant extracts viz; Nicotiana tabacum L., Nicotiana rustica L., Azadirachta indica A. Juss., Thuja orientalis L., and Melia azedarach L. against Callosobruchus maculatus L. Plant species extracts were applied at six different concentrations, i.e., 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0% in four replications. The phytochemical analyses of ethanolic extracts of five plant species showed variable amounts of phytochemicals i.e., alkaloids, flavonoids, saponins, diterpenes, phytosterol, and phenols. Total phenolic and flavonoid compounds were also observed. The efficacy of A. indica was highest, characterized by the lowest infestation rate (16.65%), host seed weight loss (7.85%), mean oviposition (84.54), and adult emergence (58.40%). In contrast, T. orientalis was found to be the least effective against C. maculatus, with the highest infestation rate of 25.60%, host seed weight loss of 26.73%, mean oviposition of 117.17, and adult emergence rate of 82.01%. Probit analysis was performed by estimating LC50 and LC90 . The toxicity percentages of N. tabacum (LC50 = 0.69%, LC90 = 14.59%), N. rustica (LC50 = 0.98%, LC90 = 22.06%), and A. indica (LC50 = 1.09%, LC90 = 68.52%) were notable in terms of the lower LC50 and LC90 values after the 96-h exposure period against C. maculatus. Repellency was assessed by using the area preference and filter paper method. The repellency of C. maculatus on plant extracts increased with the increasing dose and time, such that it was the highest after 48 h. Likewise, at a 3% concentration, A. indica demonstrated 100.00% (Class-V) repellency followed by N. tabacum (96.00%, Class-V), N. rustica (74%, Class-IV), M. azedarach (70.00%, Class-IV), and T. orientalis (68.00%, Class-IV). Based on the findings of this study, we recommend integrating N. rustica, N. tabacum, A. indica, and M. azedarach for effective management of C. maculatus and highlight the potential of these plant species in the formulation of new biocidal agents. [ABSTRACT FROM AUTHOR]- Published
- 2024
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