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The production of indigo, primarily used by the denim industry, increases year by year, and is mainly of synthetic origin. The textile industry, on which its production depends, is responsible for 10% of greenhouse gases and 20% of water pollution. However, the source of this pigment/colorant, mainly based on petrochemistry, remains a key issue today. Extracting indigo from plants is becoming a popular answer and requires an understanding and evaluation of the entire process, from raw material to pigment recovery. In this study, the indigotin precursor, indoxyl, derived from the hydrolysis of O-glycosides biomass extracted in water, was oxidized to obtain the desired pigment. This step is the most sensitive, as variations have been observed during this phase. Consequently, the standardization of the oxidation process was established to determine the extract capacity to consistently produce the blue dye pigment. Partial hydrolysis of the O-glycosides, the indoxyl precursors, was identified as a factor causing this yield variability in the obtained extracts. Once the precursors were fully chemically hydrolyzed, plants harvested during summer and during a freezing period showed a similar capacity to produce indigotin, with values of 412 ± 25 ppm and 379 ± 0 ppm, respectively. This result showed that in freezing conditions, the enzymatic material was not available, resulting in the lack of indigotin formation. To address the use of oxidation in an alkaline medium, a spontaneous oxidation method was proposed. This method produced a purer indigotin pigment, with a 21.6% purity compared to 5.9% purity using air-mediated oxidation in an alkaline medium. [ABSTRACT FROM AUTHOR]

Indigo quality is determined by its indigotin content. Another quality indicator is colour. For an evaluation of species, indigo samples from Indigofera tinctoria, Indigofera suffruticosa, Indigofera arrecta, Persicaria tinctoria, Strobilanthes cusia and Wrightia laevis cultivated in Austria and China were visually classified and analysed spectrophotometrically and using a L*a*b* measuring device. In addition to a standardised hot-extraction method without lime, some samples were extracted simulating traditional methods at ambient temperatures using lime. The highest indigotin contents were achieved with Indigofera arrecta (55%, Austria) and Strobilanthes cusia (56%, China). There were no statistically significant differences between the indigo extraction yields of the species cultivated in Austria, but Indigofera arrecta and Persicaria tinctoria had statistically significantly higher indigotin extraction yields than Indigofera tinctoria and Indigofera suffruticosa. From the species extracted in China, Strobilanthes cusia showed higher values in all parameters than Indigofera tinctoria, Indigofera suffruticosa and Wrightia laevis. Compared with the standardised method, the method simulating local practice yielded more indigo but had a lower indigotin content; the indigotin extraction yields did not differ greatly. L*a*b* values enabled precise estimations of the indigotin content, making it an interesting option for quality control, as inexpensive, easy-to-handle L*a*b* measuring instruments have become available. [ABSTRACT FROM AUTHOR]

The leaf extract of Indigofera linnaei Ali, an Indian medicinal plant, was utilized in the synthesis of copper oxide nanoparticles (CuO-NPs). Green chemistry is a safe and cost-effective method for the synthesis of nanoparticles using plant extracts. The synthesis of CuO NPs was confirmed using ultraviolet–visible (UV-visible) spectrum λ-max data with two peaks at 269 and 337 nm. Different functional groups were identified using Fourier-transform infrared spectroscopy (FT-IR). X-ray diffraction (XRD) was used to confirm the crystalline structure of the CuO-nanoparticles. Scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analyses were performed to examine the surface morphology and elemental composition of the biosynthesized CuO-NPs. Furthermore, the synthesized CuO-NPs exhibited antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecalis. Additionally, they exhibited a good insecticidal effect on Culex quinquefasciatus larvae, with low LC50 55.716 µg/mL and LC90 123.657 µg/mL values. The CuO-NPs inhibited human breast cancer cells in a concentration-dependent manner, with an IC50 value of 63.13 µg/mL. [ABSTRACT FROM AUTHOR]

Simple Summary: Indigo waste is a by-product of the processing of natural indigo dye. Indigo waste could be utilized as a protein source in ruminant rations, which would reduce the cost of feed. We evaluated the effects of the inclusion of indigo waste in concentrate diets on the feed utilization, rumen fermentation, hematology, immune function and growth performance of growing beef cattle. The present findings suggest that the inclusion of indigo waste at low levels in concentrate diets maintains feed intake, digestibility, rumen fermentation or growth performance in growing beef cattle without affecting hematology or immune function. This experiment was conducted to assess the effect of indigo waste on the feed intake, digestibility, rumen fermentation, hematology, immune response and growth performance in growing beef cattle. Twenty crossbred beef cattle with an initial body weight (BW) of 145 ± 11 kg were fed four levels of indigo waste for 90 days in a trial. Additions of indigo waste at 0%, 10%, 20% and 30% in a concentrate diet using a completely randomized design (CRD). Cattle were fed concentrate at 1.8% BW, with rice straw fed ad libitum. The concentrate intake decreased linearly (p = 0.01) with the addition of indigo waste. The supplementation with indigo waste reduced dry matter (DM) and organic matter (OM) digestibility cubically (p = 0.03 and p = 0.02, respectively), while increasing neutral detergent fiber (NDF) digestibility cubically (p = 0.02). The final BW of beef cattle decreased linearly (p = 0.03) with the addition of indigo waste. The inclusion of indigo waste decreased the average daily gain (ADG) and gain-to-feed ratio (G:F) linearly (p < 0.01) from 0 to 90 days. The nutrient digestibility, ADG and G:F of beef cattle fed 10% indigo waste in the diet was similar when compared with the control (0% indigo waste). The ruminal pH, ammonia-nitrogen (NH3-N) and total volatile fatty acid (VFA) concentrations were similar among treatments (p > 0.05). The proportion of acetate increased linearly (p < 0.01) but propionate decreased linearly (p < 0.01), resulting in an increase in the acetate to propionate ratio (p < 0.01) when cattle were fed with indigo waste supplementation. Increasing indigo waste levels did not influence blood urea nitrogen (BUN) levels, hematological parameters or immune responses (IgA, IgM and IgG) (p > 0.05). In conclusion, the inclusion of indigo waste at 10% in a concentrate diet did not have a negative effect on feed intake, nutrient digestibility, rumen fermentation, hematology, immune function or growth performance in growing beef cattle. [ABSTRACT FROM AUTHOR]

Based on vegetation-soil nutrient monitoring data under different precipitation conditions, this study investigated the impact of precipitation changes on the ecological restoration process of disturbed slopes. Precipitation change, to a certain extent, changed the carbon (C), nitrogen (N) and phosphorus (P) content and the stoichiometric ratio of the soil–plant system. With the increase of the weekly precipitation from 10 to 20 mm, the C content of Cynodon and Indigofera Amblyantha Craib on each slope gradually increased, increased by 8.69% and 4.28%, respectively, compared with the initial recovery period, and the N/P of Cynodon increased from 3.81 to 4.94, and the N limit gradually decreased, while the limit of P increased continuously. The efficiency of the coordinated utilization of N and P of the Indigofera Amblyantha Craib increased, which had a certain adaptability to changes in precipitation. The C/N and C/P in the soil first increased and then decreased, and reached the peak at the slope of 15 mm precipitation, while the N/P fluctuated around 0.35 overall. N was an important element restricting the growth of grass, while P was an important influencing element limiting the growth of shrubs. This also showed that soil C, N and P had a good promoting effect on plant growth, and the self-regulating nutrient utilization strategies of different growth forms of plants under different precipitation conditions differed. There was a coupling effect in the contents of C, N, P and their stoichiometric ratio in the soil–plant system, and stoichiometry and elastic ecological interactions jointly controlled the supply and demand of elements in the plants, but there was no consistent temporal pattern of nutrient ecological stoichiometric ratio in the plant–soil system during the recovery process, thus requiring further research and evaluation. [ABSTRACT FROM AUTHOR]

Indigofera linifolia is a medicinally important plant, and by virtue of its rich phytochemical composition, this plant is widely used as essential component in traditional medication systems. Due to its wide range of medicinal applications, the extract-loaded chitosan (Ext+Ch), extract-loaded PEG (Ext+PEG), and extract-loaded locust bean gum (Ext+LGB) nanoparticles (NPs) were prepared in the present study. The prepared NPs were then evaluated for their antibacterial, antioxidant, and antidiabetic potentials. Antibacterial activities of the crude extract and the synthesized NPs were performed following standard procedures reported in the literature. The antioxidant capabilities of extract and NPs were evaluated using DPPH free radical scavenging assay. The antidiabetic potential of the samples was evaluated against α-amylase and α-glucosidase. Ext+PEG NPs showed more potent antibacterial activity against the selected strains of bacteria with the highest activity against Escherichia coli. The lowest antibacterial potential was observed for Ext+LGB NPs. The Ext+LGB NPs IC50 value of 39 μg/mL was found to be the most potent inhibitor of DPPH free radicals. Ext+LGB NPs showed a greater extent of inhibition against α-glucosidase and α-amylase with an IC50 of 83 and 78 μg/mL, whereas for the standard acarbose the IC50 values recorded against the mentioned enzymes were 69 and 74 μg/mL, respectively. A high concentration of phenolics and flavonoids in the crude extract was confirmed through TPC and TFC tests, HPLC profiling, and GC–MS analysis. It was considered that the observed antibacterial, antidiabetic, and antioxidant potential might be due the presence of these phenolics and flavonoids detected. The plant could thus be considered as a potential candidate to be used as a remedy of the mentioned health complications. However, further research in this regard is needed to isolate the exact responsible compounds of the observed biological potentials exhibited by the crude extract. Further, toxicity and pharmacological evaluations in animal models are also needed to establish the safety or toxicity profile of the plant. [ABSTRACT FROM AUTHOR]

This is the first account of fatal toxicity in horses resulting from grazing on the pasture plant creeping indigo, Indigofera spicata, on the island of Oahu, in the Hawaiian Islands. A survey in the town of Waialua on the north shore of Oahu island indicated that creeping indigo is common and abundant on grazing lands during the rainy season and requires intensive chemical and physical control measures. Four pastures were surveyed where ranchers reported mortality of more than 17 horses since 2020. We document these incidents to alert state animal and livestock support officials, groups monitoring invasive species, and horse owners regarding the problem of this noxious weed and to support breeders with information to confront its invasiveness. Herbicide treatment is not economically feasible, and breeders opted to physically uproot the plants from the paddocks and restrain horses to clear pastures as they were eliminating the plants. We urge state officials for a long-term control strategy to reduce the problems associated with this weed. [ABSTRACT FROM AUTHOR]

High Performance Liquid Chromatography coupled to a Diode-Array-Detector (HPLC-DAD) is used to investigate samples which were extracted from ancient Egyptian textiles (4th–5th c. AD) of the Museum of Faculty of Archaeology, Cairo University. Madder is identified in several samples. According to semi-quantitative results, which are obtained from HPLC peak areas measured at 254 nm, madder that is rich in purpurin and poor in alizarin is identified in samples which were treated (i) only with madder and (ii) with madder and either indigo/woad (Indigofera species and other/Isatis tinctoria L.) or weld (Reseda luteola L.). The madder dye used in these samples could have been originated from Rubia peregrina L. However, the possible use of Rubia tinctorum L. (or other plants of the Rubiaceae family) by the Egyptian dyers cannot be ruled out, particularly if methods were developed by the ancient dyers to affect and control the relative composition of madder dye. The HPLC peak area ratio of alizarin versus purpurin is very high (>2.2) for samples which were treated with madder (probably originated from R. tinctorum) and a tannin source. Finally, in some samples, only indigoid dyes (indigo/woad) are identified. [ABSTRACT FROM AUTHOR]

Indigofera pseudotinctoria Mats is an agronomically and economically important perennial legume shrub with a high forage yield, protein content and strong adaptability, which is subject to natural habitat fragmentation and serious human disturbance. Until now, our knowledge of the genetic relationships and intraspecific genetic diversity for its wild collections is still poor, especially at small spatial scales. Here amplified fragment length polymorphism (AFLP) technology was employed for analysis of genetic diversity, differentiation, and structure of 364 genotypes of I. pseudotinctoria from 15 natural locations in Wushan Montain, a highly structured mountain with typical karst landforms in Southwest China. We also tested whether eco-climate factors has affected genetic structure by correlating genetic diversity with habitat features. A total of 515 distinctly scoreable bands were generated, and 324 of them were polymorphic. The polymorphic information content (PIC) ranged from 0.694 to 0.890 with an average of 0.789 per primer pair. On species level, Nei's gene diversity (Hj), the Bayesian genetic diversity index (HB) and the Shannon information index (I) were 0.2465, 0.2363 and 0.3772, respectively. The high differentiation among all sampling sites was detected (FST = 0.2217, GST = 0.1746, G'ST = 0.2060, өB = 0.1844), and instead, gene flow among accessions (Nm = 1.1819) was restricted. The population genetic structure resolved by the UPGMA tree, principal coordinate analysis, and Bayesian-based cluster analyses irrefutably grouped all accessions into two distinct clusters, i.e., lowland and highland groups. The population genetic structure resolved by the UPGMA tree, principal coordinate analysis, and Bayesian-based cluster analyses irrefutably grouped all accessions into two distinct clusters, i.e., lowland and highland groups. This structure pattern may indicate joint effects by the neutral evolution and natural selection. Restricted Nm was observed across all accessions, and genetic barriers were detected between adjacent accessions due to specifically geographical landform. [ABSTRACT FROM AUTHOR]

A strategy that has the potential to contribute to the achievement of the targets established under SDG 8 ("Decent Work and Economic Growth") involves the development of sustainable tourism to create productive job and livelihood opportunities and to promote local culture and products. In the province of East Nusa Tenggara (NTT), Indonesia, ikat weaving is an integral part of the cultural heritage of the region, with ikat characterized by unique design motives and the use of natural dyes. Unfortunately, in some areas, the use of natural dyes is diminishing as a result of competition with synthetic dyes and the limited availability of raw materials, particularly for the production of blue dye. The development of Indigofera spp. to produce natural, plant-based blue dye has significant potential to contribute to community incomes and employment. This study outlines a strategy for developing the use of Indigofera spp. as a natural dye and describes its potential for contributing to community incomes, especially on Timor Island, NTT. The study shows that weavers currently use indigo plants that grow naturally in gardens and yards. Although the community has a tradition of using these plants, informed by local wisdom, to develop the cultivation and processing of this plant through the application of the appropriate techniques, including innovative approaches to producing indigo paste, could enable the development of Indigofera spp. cultivation on a larger scale. The study shows that the availability of suitable land is high (±370 thousand ha), with 80% of the suitable land being categorized as critical. The study proposes a development based on the analysis of strengths, weaknesses, opportunities, and threats to assess the manner in which these opportunities could be leveraged with the support and participation of local communities and other stakeholders by leveraging the culture and wisdom of the community regarding the use of Indigofera spp. [ABSTRACT FROM AUTHOR]

The present study evaluated the antiulcerogenic activity and mechanisms of the aqueous (AqF 100 mg/kg) and ethyl acetate (AcF 50 mg/kg) fractions from Indigofera truxillensis leaves. This dose was selected to assess its activity on ulcer healing and its action on gastric acid and mucus secretion, prostaglandin production and antioxidant enzyme activity (superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione reductase (GSH-Rd)). Gastric ulcer was induced by absolute ethanol. Antisecretory action, mucus and prostaglandin production, healing and antioxidant enzyme activities were evaluated for both fractions. AqF and AcF significantly inhibited the gastric mucosal damage caused by ethanol. This effect was statistically significant at 100 and 50 mg/kg compared with the vehicle. Neither fraction interfered with gastric secretion. AcF increased the PGE2 production, and both fractions increased mucus production. L-NAME did not alter the gastroprotection exerted by the fractions, but N-ethylmaleimide attenuated only AcF. In the ischemia/reperfusion model both fractions inhibited the mucosal damage. AcF increased SOD, GSH-Px and GSH-Rd activity, but AqF increased only SOD and GSH-Px. In the acetic acid-induced ulcer model AcF only accelerated ulcer healing. These results showed that Indigofera truxillensis acted as a gastroprotective agent, stimulating protective factors and antioxidants enzymes. [ABSTRACT FROM AUTHOR]

The leguminous plant species, Indigofera linnaei and Indigofera spicata are distributed throughout the rangeland regions of Australia and the compound indospicine (L-2-amino-6-amidinohexanoic acid) found in these palatable forage plants acts as a hepatotoxin and can accumulate in the meat of ruminant livestock and wild camels. In this study, bovine rumen fluid was cultivated in an in vitro fermentation system provided with Indigofera spicata plant material and the ability of the resulting mixed microbial populations to degrade indospicine was determined using UPLC–MS/MS over a 14 day time period. The microbial populations of the fermentation system were determined using 16S rRNA gene amplicon sequencing and showed distinct, time-related changes occurring as the rumen-derived microbes adapted to the fermentation conditions and the nutritional substrates provided by the Indigofera plant material. Within eight days of commencement, indospicine was completely degraded by the microbes cultivated within the fermenter, forming the degradation products 2-aminopimelamic acid and 2-aminopimelic acid within a 24 h time period. The in vitro fermentation approach enabled the development of a specifically adapted, mixed microbial population which has the potential to be used as a rumen drench for reducing the toxic side-effects and toxin accumulation associated with ingestion of Indigofera plant material by grazing ruminant livestock. [ABSTRACT FROM AUTHOR]

Indigo is a plant dye that has been used as an important dye by various ancient civilizations throughout history. Today, due to environmental and health concerns, plant indigo is re-entering the market. Strobilanthes cusia (Nees) Kuntze is the most widely used species in China for indigo preparation. However, other species under Strobilanthes have a similar feature. In this work, 12 Strobilanthes spp. were analyzed using electrochemical fingerprinting technology. Depending on their electrochemically active molecules, they can be quickly identified by fingerprinting. In addition, the fingerprint obtained under different conditions can be used to produce scattered patter and heatmap. These patterns make plant identification more convenient. Since the electrochemically active components in plants reflect the differences at the gene level to some extent, the obtained electrochemical fingerprints are further used for the discussion of phylogenetics. [ABSTRACT FROM AUTHOR]

Weed management is an important issue since weeds directly compete with crop plants for space, nutrients; serve as habitat for insect pests and diseases, and can create a significant annual reduction in crop productivity. This study focused on evaluating the contribution of the secondary metabolites of the fruit pulp of Couroupita guianensis Aubl. for its potential growth inhibitory effect. Crude extracts of C. guianensis fruit pulp were collected with different solvents and applied to test plants in petri dishes. The crude extracts of methanol and 1% dimethyl sulfoxide (DMSO) showed potential growth inhibitions with the 50% effective concentration (EC50) of 223 and 229 µg/mL in the bioassay experiment. In the greenhouse pot experiment, soil incorporated with oven-dried fruit pulp of C. guianensis was evaluated on cultivated plant species including Lactuca sativa L., Trifolium repens L., Medicago sativa L., Lolium multiflorum Lam., and Phleum pratense L. The incorporation of dried fruit pulp of C. guianensis into soil reduced shoot and root lengths and the germination percentage of test plants. It was observed that the monocot plants were more affected than the dicot plants. The fruit pulp of C. guianensis was subjected to reversed-phase high-performance liquid chromatography (HPLC) analysis to identify the active compounds. Indigo, identified as one of the candidate compounds of the C. guianensis, had high specific activity (i.e., strong inhibitory activity) in a phytotoxicity bioassay and could explain through the total activity concept the growth inhibitory effect of the C. guianensis on test plants. The results suggested that indigo has plant growth inhibitory effect, indicating the allelopathic potential of C. guianensis, which could be exploited in sustainable weed management. [ABSTRACT FROM AUTHOR]

Indigofera zollingeriana Miq (I.zollingeriana) is a widely grown tree in Vietnam. It is used to cure various illnesses. The purpose of this study was to investigate the chemical constituents of an I. zollingeriana extract and test its anticancer activity on hepatocellular cells (Huh7 and HepG2). The experimental results of the analysis of the bioactive compounds revealed that β-sitosterol (β-S) and β-sitosterol-glucoside (β-SG) were the main ingredients of the I.zollingeriana extract. Regarding anticancer activity, the β-S and β-SG of I. zollingeriana were found to exhibit cytotoxic effects against HepG2 and Huh7 cells, but not against normal human primary fibroblasts. The β-S was able to inhibit the proliferation of HepG2 and Huh7 cells in a dose-dependent manner with half-maximal inhibitory concentration (IC50) values of 6.85 ± 0.61 µg/mL and 8.71 ± 0.21 µg/mL, respectively (p < 0.01), whereas the β-SG IC50 values were 4.64 ± 0.48 µg/mL for HepG2 and 5.25 ± 0.14 µg/mL for Huh7 cells (p < 0.01). Remarkably, our study also indicated that β-S and β-SG exhibited cytotoxic activities via inducing apoptosis and activating caspase-3 and -9 in these cells. These findings demonstrated that β-S and β-SG from I.zollingeriana could potentially be developed into promising therapeutic agents to treat liver cancer. [ABSTRACT FROM AUTHOR]

In vitro experiments have demonstrated that camel foregut-fluid has the capacity to metabolize indospicine, a natural toxin which causes hepatotoxicosis, but such metabolism is in competition with absorption and outflow of indospicine from the different segments of the digestive system. Six young camels were fed Indigofera spicata (337 µg indospicine/kg BW/day) for 32 days, at which time three camels were euthanized. The remaining camels were monitored for a further 100 days after cessation of this indospicine diet. In a retrospective investigation, relative levels of indospicine foregut-metabolism products were examined by UHPLC-MS/MS in plasma, collected during both accumulation and depletion stages of this experiment. The metabolite 2-aminopimelamic acid could be detected at low levels in almost all plasma samples, whereas 2-aminopimelic acid could not be detected. In the euthanized camels, 2-aminopimelamic acid could be found in all tissues except muscle, whereas 2-aminopimelic acid was only found in the kidney, pancreas, and liver tissues. The clearance rate for these metabolites was considerably greater than for indospicine, which was still present in plasma of the remaining camels 100 days after cessation of Indigofera consumption.