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4 results on '"Huayan Pu"'

1. Simultaneously Quantifying Both Young’s Modulus and Specific Membrane Capacitance of Bladder Cancer Cells with Different Metastatic Potential

Author

Na Liu, Jun Luo, Huayan Pu, Tao Yue, Mengying Leng, Liang Dong, Liu Yuanyuan, Yan Peng, and Shaorong Xie

Subjects
lcsh:Mechanical engineering and machinery, Cell, single cell analysis, Young's modulus, 02 engineering and technology, Article, 03 medical and health sciences, symbols.namesake, 0302 clinical medicine, Single-cell analysis, medicine, lcsh:TJ1-1570, Young’s modulus, Electrical and Electronic Engineering, Grading (tumors), specific membrane capacitance, Membrane potential, Bladder cancer, Chemistry, Mechanical Engineering, Cancer, micropipette aspiration, 021001 nanoscience & nanotechnology, medicine.disease, medicine.anatomical_structure, Control and Systems Engineering, 030220 oncology & carcinogenesis, Cancer cell, Cancer research, symbols, bladder cancer, 0210 nano-technology
Abstract

Both Young&rsquo, s modulus and specific membrane capacitance (SMC) are two important physical parameters for characterizing cell status. In this paper, we utilized a thin-neck-micropipette aspiration system to simultaneously quantify Young&rsquo, s modulus and SMC value of six types of cell lines in different progression grades, which include four grades from the lowest metastatic potential G1 to the highest potential G4. We investigated how these two physical properties possess heterogeneities in bladder cancer cells with different grades and what roles they might play in grading bladder cancer. The characterization results of these cells of different cancer grades is linearly correlated with the cancer grades, showing that the Young&rsquo, s modulus is negatively linearly correlated with bladder cancer grades, while SMC shows a positive linear correlation. Furthermore, the combination of these two physical properties on a scatter diagram clearly shows the cell groups with different cancer grades, which means that this combination could be a potential tumor grading marker to identify cancer cells with different metastatic potential.

Published
2020

2. A Novel Biodegradable Multilayered Bioengineered Vascular Construct with a Curved Structure and Multi-Branches

Author

Yan Peng, Tao Yue, Liu Yuanyuan, Jun Luo, Weijian Jiang, Shaorong Xie, Yi Zhang, Na Liu, Huayan Pu, and Zhong Songyi

Subjects
3d printed, Materials science, lcsh:Mechanical engineering and machinery, Cardiovascular research, 02 engineering and technology, 030204 cardiovascular system & hematology, Cell morphology, Article, 03 medical and health sciences, enzymatically-crosslinked, 0302 clinical medicine, Tissue engineering, Fabrication methods, bioengineered vascular constructs, curved structure, lcsh:TJ1-1570, Electrical and Electronic Engineering, Tissue engineered, Mechanical Engineering, Construct (python library), 021001 nanoscience & nanotechnology, Control and Systems Engineering, multi-branches, tissue engineering, 0210 nano-technology, Wall thickness, Biomedical engineering
Abstract

Constructing tissue engineered vascular grafts (TEVG) is of great significance for cardiovascular research. However, most of the fabrication techniques are unable to construct TEVG with a bifurcated and curved structure. This paper presents multilayered biodegradable TEVGs with a curved structure and multi-branches. The technique combined 3D printed molds and casting hydrogel and sacrificial material to create vessel-mimicking constructs with customizable structural parameters. Compared with other fabrication methods, the proposed technique can create more native-like 3D geometries. The diameter and wall thickness of the fabricated constructs can be independently controlled, providing a feasible approach for TEVG construction. Enzymatically-crosslinked gelatin was used as the material of the constructs. The mechanical properties and thermostability of the constructs were evaluated. Fluid-structure interaction simulations were conducted to examine the displacement of the construct&rsquo, s wall when blood flows through it. Human umbilical vein endothelial cells (HUVECs) were seeded on the inner channel of the constructs and cultured for 72 h. The cell morphology was assessed. The results showed that the proposed technique had good application potentials, and will hopefully provide a novel technological approach for constructing integrated vasculature for tissue engineering.

Published
2019

3. Interactive Micromanipulation of Picking and Placement of Nonconductive Microsphere in Scanning Electron Microscope.

Author

Ning Cao, Shaorong Xie, Zhizheng Wu, Mei Liu, Hengyu Li, Huayan Pu, Jun Luo, and Zhenbang Gong

Subjects
MICROSPHERES, SCANNING electron microscopes
Abstract

In this paper, classified theoretical models, consisting of contact with and placement of microsphere and picking operations, are simplified and established to depict the interactive behaviors of external and internal forces in pushing manipulations, respectively. Sliding and/or rolling cases, resulting in the acceleration of micromanipulations, are discussed in detail. Effective contact detection is achieved by combining alterations of light-shadow and relative movement displacement between the tip-sphere. Picking operations are investigated by typical interactive positions and different end tilt angles. Placements are realized by adjusting the proper end tilt angles. These were separately conducted to explore the interactive operations of nonconductive glass microspheres in a scanning electron microscope. The experimental results demonstrate that the proposed contact detection method can efficiently protect the end-tip from damage, regardless of operator skills in initial positioning operations. E-beam irradiation onto different interactive positions with end tilt angles can be utilized to pick up microspheres without bending the end-tip. In addition, the results of releasing deviations away from the pre-setting point were utilized to verify the effectiveness of the placement tilt angles. [ABSTRACT FROM AUTHOR]

Published
2017
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4. The Disturbing Effect of the Stray Magnetic Fields on Magnetoimpedance Sensors.

Author

Tao Wang, Yong Zhou, Chong Lei, Shaotao Zhi, Lei Guo, Hengyu Li, Zhizheng Wu, Shaorong Xie, Jun Luo, and Huayan Pu

Subjects
GIANT magnetoresistance, AMORPHOUS substances, IRON, MAGNETIC fields, MAGNETIC sensors, FERROMAGNETISM
Abstract

The disturbing effect of the stray magnetic fields of Fe-based amorphous ribbons on the giant magnetoimpedance (GMI) sensor has been investigated systematically in this paper. Two simple methods were used for examining the disturbing effect of the stray magnetic fields of ribbons on the GMI sensor. In order to study the influence of the stray magnetic fields on the GMI effect, the square-shaped amorphous ribbons were tested in front, at the back, on the left and on the top of a meander-line GMI sensor made up of soft ferromagnetic films, respectively. Experimental results show that the presence of ribbons in front or at the back of GMI sensor shifts the GMI curve to a lower external magnetic field. On the contrary, the presence of ribbons on the left or on the top of the GMI sensor shifts the GMI curve to a higher external magnetic field, which is related to the coupling effect of the external magnetic field and the stray magnetic fields. The influence of the area and angle of ribbons on GMI was also studied in this work. The GMI sensor exhibits high linearity for detection of the stray magnetic fields, which has made it feasible to construct a sensitive magnetometer for detecting the typical stray magnetic fields of general soft ferromagnetic materials. [ABSTRACT FROM AUTHOR]

Published
2016
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