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Fruit firmness in sweet cherries (Prunus avium L.) is a critical quality parameter highly valued by consumers as it is associated with fruit freshness. In general, firm fruit also cope better with storage and handling. Gibberellic acid (GA) is commonly used by sweet cherry producers to increase firmness, soluble solids content and fruit size. This study evaluated the effects of GA on the rheological properties of sweet cherry fruit at harvest and postharvest storage. Specifically, GA's influence on susceptibility to mechanical damage during handling was evaluated. The following GA treatments were applied to two sweet cherry cultivars 'Bing' and 'Lapins': T0, control, T30—GA at 15 ppm applied at pit-hardening and straw-colour stages; T45—GA at 25 ppm at pit-hardening and GA at 20 ppm at straw-colour; and T60—GA at 30 ppm applied at pit-hardening and straw-colour. The results indicate that GA delayed harvest by two to four days in both cultivars, with 'Lapins' also showing a significant increase in fruit size. Regardless of spray concentration, GA increased the modulus of elasticity and fruit resistance evaluated as stress at the maximum point at harvest. These effects persisted after 35 days of storage at 0 °C and an additional three days of shelf-life at 15 °C. While the strain or deformation capacity of the fruit at bioyield at harvest was constant across treatments, it was, however, lower in the GA-treated fruit than in the controls during storage at 0 °C under the high-humidity conditions of modified atmosphere packaging. The less mature fruit harvested at colour 3.0 (red/mahogany) were stiffer (reduced deformation) and more sensitive to induced mechanical injury than the fruit harvested later at colour 3.5 (mahogany). The GA treatments increased fruit resistance to damage without increasing tissue deformability. Other questions associated with stiffer tissues and lower deformability during storage at 0 °C under high humidity should be further studied, specifically cultivars that are naturally high in box-cracking sensitivity during storage. [ABSTRACT FROM AUTHOR]

Predicting the post-storage quality of cherry fruits is crucial for determining their suitability for long-distance shipping or domestic distribution. This study aimed to forecast key quality attributes of Staccato sweet cherries after storage, simulating shipping conditions, by analyzing spring soil, leaf, fruitlet, and at-harvest data from thirty orchards in the Okanagan Valley, British Columbia, Canada, over two years. A support vector machine (SVM) was used to predict post-storage variables, with pre-harvest and at-harvest data selected by a genetic algorithm. The SVM accurately predicted soluble solids (R2 = 0.88), firmness (R2 = 0.83), and acidity (R2 = 0.79) after four weeks of storage, as well as visual disorders like slip skin and stem browning. Spring soil properties (Ca, Mg), leaf (N, Ca, Mg, Fe, Zn, B), and fruitlet data (N, Ca, Mg, B) were key predictors. Leaf Ca was vital for firmness and total soluble solids (TSS) prediction, while N in leaves and fruitlets influenced firmness, acidity, and disorders. Leaf Zn helped predict weight and acidity/TSS ratio, and Mg impacted fruit color. Pre-harvest leaf nutrition measured 3–4 weeks before harvest, proved most effective in predicting post-storage quality. [ABSTRACT FROM AUTHOR]

The physico-chemical properties of potato tubers change with age, which can affect not only the quality of the tubers but also the quality and shelf-life of fresh-cut potatoes (FCP) produced from older tubers. One of the methods that has proven useful for extending the shelf-life of some foods is ultraviolet-C (UV-C) irradiation. This study investigated whether UV-C irradiation can mitigate the negative effects of tuber age. FCP from tubers after storage (1 and 9 months), vacuum-packaged, were irradiated with UV-C (2.70 kJ m−2) and stored (6 °C/15 days). The total aerobic mesophilic bacteria count (TAMBC), total and soluble solids content, pH, firmness, color, chlorogenic acid, reducing sugars, sensory properties of raw FCP and subsequently boiled and fried FCP, and acrylamide content in fried FCP were monitored. UV-C irradiation of FCP does not affect all parameters equally for both tuber ages, but was generally effective in reducing TAMBC, and UV-C-treated samples reached a shelf-life of 15 days. UV-C irradiation improved the sensory properties of raw and thermally processed FCP at both ages. UV-C irradiation caused an increase in acrylamide content in fried FCP, regardless of. the tubers age, but all samples were safe in this respect. [ABSTRACT FROM AUTHOR]

Parsnip, a root vegetable from the Apiaceae family, is rich in dietary fiber, pectin, and starch but remains relatively unfamiliar in South Korea. This study investigated the physicochemical properties of two Korean-grown parsnip cultivars, 'Warrior' and 'Albion', focusing on their organic acid and sugar compositions. The 'Warrior' cultivar has higher firmness and water content but lower SSC compared to 'Albion'. In 'Warrior', malic and lactic acids were the main organic acids, while 'Albion' had predominant oxalic and malic acids. Malic acid was also the primary organic acid in the inedible parts of 'Warrior', and oxalic acid in 'Albion'. In the edible parts of both cultivars, sucrose was identified as the main sugar. In 'Warrior', the levels were 88.59%, 90.35%, and 79.13% in the cortex, pith, and skin, respectively, while in 'Albion', the levels were 88.56%, 64.40%, and 67.39%. 'Warrior' showed higher total sugar content in its cortex (6.66%) compared to 'Albion' (3.67%). These results highlight the beneficial compounds in parsnips and suggest their potential for improving dietary strategies and health. [ABSTRACT FROM AUTHOR]

Xylella fastidiosa subsp. pauca (Xfp) is a significant threat to Mediterranean agriculture, particularly impacting olive trees in southern Italy, causing Olive Quick Decline Syndrome. Resistant olive cultivars, such as 'Leccino' and 'Fs-17', have been identified as alternatives to restore the oliviculture within the infected areas. 'Frantoio' and 'Cipressino' are included in ongoing studies on genetic resistance to Xfp. The mechanization of olive harvesting is essential for reducing production costs in the olive oil sector. Two systems, trunk shakers and over-the-row machines, are used depending on the tree density and canopy structure, with super-high-density systems offering advantages in terms of cost and efficiency. This study investigates the feasibility of using simple and non-destructive indices to assess the optimal mechanical harvesting time. Different physical ripening indices, including detachment force, fresh weight, pigmentation, and firmness, were measured on four olive cultivars ('Fs-17', 'Leccino', 'Frantoio', 'Cipressino') in southern Italy over two years. The study found that the pigmentation index had a strong relationship with the detachment index, particularly for 'Fs-17', and 'Leccino', providing a reliable non-destructive measure for optimal harvesting time. The results indicate that the optimal harvesting times for mechanical harvesting are early September for 'Cipressino', early October for 'Fs-17', and mid-October for 'Frantoio' and 'Leccino'. [ABSTRACT FROM AUTHOR]

The effect of N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU) on fruit splitting of 'Murcott' tangor fruit (Citrus reticulate × Citrus sinensis) was analyzed throughout two successive seasons during 2020 and 2021. 'Murcott' trees were treated with various concentrations of CPPU, 1.0, 5.0, and 10.0 mg·L−1, which significantly reduced the splitting of fruit compared with the control fruit and the gibberellic acid (GA3)-treated fruit (20.0 mg·L−1). The fruit treated with 5.0 mg·L−1 CPPU exhibited higher levels of fruit weight, longitudinal diameter, transverse diameter, peel strength, and peel thickness compared to the control fruit. In addition, the tree sprayed with 5.0 mg·L−1 CPPU had the greatest number of cellular layers and cells at the stylar end calculated in fruit. However, there were no other fruit quality characteristics significantly affected by CPPU and GA3 treatments. [ABSTRACT FROM AUTHOR]

Understanding the fundamental light-sample interaction process is a crucial step toward the development of vibrational spectroscopy to determine fruit texture (i.e., firmness). This study aimed to investigate the effect of pectin constitution, including total pectin, water-soluble pectin, protopectin contents, and protopectin index (PI), on the optical properties and firmness of 'Baifeng' and 'Xiahui 8' peach flesh at the different softening degrees during postharvest storage of 6 days at 20 °C. The firmness of 'Baifeng' and 'Xiahui 8' peaches significantly (p < 0.05) changed with a decreasing rate from 90.3% to 92.2%. Peach firmness of these two cultivars correlated well with PI contents (r > 0.912) and showed good internal correlations with optical scattering properties. The light absorption coefficient (μa) and reduced scattering coefficient (μ's) at 600–1600 nm were measured using a single integrating sphere system combined with an inversion algorithm. This relationship of μa and μ's with peach firmness and pectin constitution was first analyzed. Notably, the specific μ's at 660 nm, 950 nm, 1203 nm, and 1453 nm showed a satisfactory prediction of peach firmness and PI of 'Xiahui 8' (R2 ≥ 0.926) and 'Baifeng' peaches (R2 ≥ 0.764), respectively. Furthermore, the prediction models were established based on partial least squares regression coupled with optical properties, and considerable prediction performances were obtained for tissue firmness (Rp2 ≥ 0.863) and PI based on μ's (Rp2 ≥ 0.802). Consequently, these results further verified that the spectroscopic prediction model for peach firmness could be related to the high correlations between PI in tissues and their optical scattering properties. Future research interests could include the development of optical absorption and scattering sensors for rapid and efficient determination of peach firmness. [ABSTRACT FROM AUTHOR]

Textural softening is a major factor that limits the storage potential of fruit. Fresh produce markets incur severe financial losses due to excessive fruit softening. The application of preservation strategies aimed at mitigating fruit softening is crucial for optimising the marketability of fruit. Proposed preservation strategies include ecofriendly treatments, namely, hexanal, edible coatings, heat treatments, ozone and UV-C irradiation. These treatments optimise firmness retention by targeting the factors that affect fruit softening, such as ethylene, respiration rates, enzymes and pathogens. This review discusses the mechanisms by which ecofriendly treatments inhibit fruit softening, providing insights into their effect on ethylene biosynthesis, cell wall metabolism and disease resistance. Although ecofriendly treatments offer a promising and sustainable approach for delaying fruit softening, the optimisation of treatment application protocols is needed to improve their efficacy in retaining fruit firmness. Studies reporting on the molecular mechanisms by which ecofriendly treatments inhibit fruit softening are limited. Future studies should prioritise proteomic and transcriptome analyses to advance our understanding of the underlying molecular mechanisms by which ecofriendly treatments delay the fruit-softening process. [ABSTRACT FROM AUTHOR]

Selenium (Se) is a nutrient whose daily intake is often below the recommended levels in people. Biofortification with Se is a method to increase this intake by raising the Se concentration in tomato fruits, an effect dependent on sources and modes of application. Additionally, Se application can promote the enhancement of other compounds in tomato fruits, altering their metabolism, which may increase the fruit's shelf life. This study aimed to determine how different strategies of applying a multi-nutrient fertilizer containing Se (SeMNF) can increase the Se content and other bioactive compounds and enhance the shelf life of tomato (Solanum lycopersicum L.) fruits. Different foliar fertilization strategies involving the use of SeMNF were evaluated in field trials conducted on commercial tomato crops. Indeterminate-growth tomatoes were used, and different Se doses and application strategies were tested. Harvesting was conducted in three phases according to fruit ripening. Each harvested fruit was assessed for the Se content, macro and micronutrients, total phenolic compounds, vitamin C, antioxidant activity, carotenoids, pH, total titratable acidity, and total soluble solids in tomato fruits. Doses of 15 g ha−1 of Se, split into three applications, increased the Se content in the fruits at 1 and 2 harvests. The application of SeMNF at Se doses above 10 g of Se ha−1 increased firmness, days of ripening, and the nutritional quality of the tomatoes (higher contents of carotenoids (+39%), lycopene (+33%), antioxidant activity (+16%), total phenolic compounds (+38%), and vitamin C (+14%) in a dose-dependent effect of the application strategy used. These results contributed to an increase in the shelf life of tomatoes, consequently reducing food waste. [ABSTRACT FROM AUTHOR]

Tomatoes are delicate and prone to damage quickly, which ultimately leads to lower quality and increased post-harvest losses. Hence, an ideal storage environment is very important to maintain the quality of tomato fruits after harvest. Therefore, this study was conducted to determine the effect of storage conditions on the quality parameters of tomato fruits. Experiments were de-signed for six storage periods (4, 8, 12, 16, 20, and 24 days) and two temperatures (12 °C and room air temperature "22 °C"). Three tomato fruit quality parameters (Brix, color, and firmness) have been selected and measured for three tomato varieties (Ghandowra-F1, Forester-F1, and Feisty-Red) grown hydroponically at three salinity levels (2.5, 6.0, and 9.5 dS m−1) of the nutrient solution. Results showed that the highest mean Brix values, for all varieties, were recorded at the highest salinity (9.5 dS m−1), and were significantly (Pr < 0.0001) higher than those at medium (6.0 dS m−1) and low (2.5 dS m−1) salinity levels. In addition, the highest fruit firmness was recorded at high salinity level (9.5 dS m−1), but there was no significant difference to that recorded at medium salinity (6.0 dS m−1). Regarding tomato fruit color, the highest average values were recorded for the Ghandowra-F1 (2.51) and Forester-F1 (2.69) varieties at medium salinity (6.0 dS m−1), while the highest average color value for the Feisty-Red variety (1.54) was obtained at high salinity (9.5 dS m−1). On the other hand, the Brix, color, and firmness of tomato fruits were significantly affected by the storage temperature. Moreover, the mean Brix values (7.66%) were slightly higher at 12 °C storage temperature compared to those at 22 °C (7.38%). In general, the fruit color values gradually increased with the storage period, especially under 22 °C storage temperature, with peak color values of 2.73, 2.70, and 1.66 recorded on the 12th day of the storage period for Ghandowra-F1, Forester-F1, and Feisty-Red, respectively. Tomato fruit firmness decreased faster with the storage period at 22 °C compared to the storage temperature of 12 °C. However, the highest average values of fruit firmness for Ghandowra-F1 (9.37 N cm−1) and Forester-F1 (9.41 N cm−1) recorded at control condition were not significantly different those recorded on the 8th day of storage at 12 °C storage temperature. By contrast, the highest average value of fruit firmness for Feisty-Red (8.85 N cm−1) recorded at control condition was not significant than that recorded on the 4th day of the storage period at 12 °C storage temperature (8.82 N cm−1). Overall, tomato fruits can be stored at 12 °C temperature for up to 20–24 days, without negative effects on fruit quality. [ABSTRACT FROM AUTHOR]

The effects of normal (NA) and controlled atmosphere (CA) storage and postharvest treatment with 1-methylcyclopropene (1-MCP) before CA storage for 5 months on the volatilome, biochemical composition and quality of ‘Golden Delicious’ (GD) and ‘Red Delicious’ (RD) apples were studied. Apples stored under NA and CA maintained and 1-MCP treatment increased firmness in both cultivars. NA storage resulted in a decrease of glucose, sucrose and fructose levels in both cultivars. When compared to CA storage, 1-MCP treatment caused a more significant decrease in sucrose levels and an increase in glucose levels. Additionally, 1-MCP-treated apples exhibited a significant decrease in malic acid content for both cultivars. All storage conditions led to significant changes in the abundance and composition of the volatilome in both cultivars. GD and RD apples responded differently to 1-MCP treatment compared to CA storage; higher abundance of hexanoate esters and (E,E)-α-farnesene was observed in RD apples treated with 1-MCP. While 1-MCP was effective in reducing (E,E)-α-farnesene abundance in GD apples, its impact on RD apples was more limited. However, for both cultivars, all storage conditions resulted in lower levels of 2-methylbutyl acetate, butyl acetate and hexyl acetate. The effectiveness of 1-MCP is cultivar dependent, with GD showing better results than RD. [ABSTRACT FROM AUTHOR]

This study evaluated the effect of salicylic acid (SA) application on yield, fruit quality, and post-harvest storage in Prunus avium subjected to deficit irrigation (DI). A field experiment with six-year-old P. avium cv. Lapins was performed under two water treatments: irrigation at 100% of crop evapotranspiration (ETc) [full irrigation (FI)] and irrigation at 60% ETc from the second fruit phase to harvest time (DI). A single 0.5 mM SA was applied to both water treatments at fruit color change. At harvest time, fruits were collected to determine yield, fruit quality, and quality during post-harvest storage (0, 10, 20, and 30 days). The DI reduced fruit yield (11%), fruit weight (8%), and caliber (6%) and increased firmness (7%) and total soluble solids (TSS) (5%) in P. avium compared with FI plants at harvest time. Our study showed that SA application recovered fruit yield (9%), fruit weight (5%), and caliber (4%), improving TSS in DI plants at day 0. Interestingly, SA application significantly reduced P. avium fruit cracking (78% in FI and 82% in DI). Fruit weight was reduced in all treatments, mainly decreasing by 14% in FI and 13% in DI plants at day 30 of post-harvest storage. Fruit weight did not change during post-harvest storage with SA, except on day 30, where a slight reduction was observed. TSS showed no significant differences during post-harvest storage for all treatments. Therefore, SA could be an interesting tool to mitigate the impact of DI on the yield and fruit quality of P. avium and to reduce fruit cracking and prolong fruit quality during post-harvest storage. [ABSTRACT FROM AUTHOR]

Tomato (Solanum lycopersicum) is a widely consumed vegetable crop with significant economic and nutritional importance. This review paper discusses the recent advancements in gene-based approaches to enhance the quality of tomatoes, particularly focusing on firmness, shelf life, and adaptations to pre- and post-harvest stresses. Utilizing genetic engineering techniques, such as Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins 9 (CRISPR/Cas9) and Transcription Activator-like Effector Nucleases (TALENs), researchers have made remarkable progress in developing tomatoes with improved traits that address key challenges faced during cultivation, storage, and transportation. We further highlighted the potential of genetic modifications in enhancing tomato firmness, thereby reducing post-harvest losses and improving consumer satisfaction. Furthermore, strategies to extend tomato shelf life through genetic interventions are discussed, emphasizing the importance of maintaining quality and freshness for sustainable food supply chains. Furthermore, the review delves into the ways in which gene-based adaptations can bolster tomatoes against environmental stresses, pests, and diseases, thereby enhancing crop resilience and ensuring stable yields. Emphasizing these crucial facets, this review highlights the essential contribution of genetic advancements in transforming tomato production, elevating quality standards, and promoting the sustainability of tomato cultivation practices. [ABSTRACT FROM AUTHOR]

Mangifera indica var Ataulfo is the main variety grown in the Soconusco region of Chiapas, Mexico and faces a decrease in firmness and weight loss due to hydrothermal treatment, in addition to non-uniform ripening and consequently a decrease in price as the harvest period lengthens. In order to improve the postharvest quality of the "Ataulfo" mango fruit, preharvest applications of aminoethoxyvinylglycine (AVG; ReTain®, a.i. 15%) were carried out in "La Norteña" with Phaeozem soil, 1500 mm of annual precipitation, and applications of agrochemicals, and in "Santa Cecilia" with Acrisol soil, 2500 mm of annual precipitation, and without agrochemicals. The treatments were: (1) Control, (2) One application 7 days before harvest (0.1 g L−1), (3) Two applications (14 days and 7 days before harvest, 0.2 g L−1) and (4) Three applications (21, 14 and 7 days before harvest, in total 0.3 g L−1), with completely randomized design in the laboratory. The results show contrasting differences between the evaluation sites, with lower weight loss (18.4%) and 3.1% more firmness in a drier climate and higher soil calcium content. The average firmness (N) on day 14 was 18.3 and with three applications of AVG it was 22.0. The °Brix at the end of the study in the control was 11.3 and on average with AVG applications it was 14.4. Three-time applications of AVG in preharvest delayed the weight loss with an increase in the maintenance of the firmness of the fruits. The weight of the fruit epidermis was lower with AVG applications, and increased with the number of applications. [ABSTRACT FROM AUTHOR]

Cerasus Humilis fruit is susceptible to rapid color changes post-harvest, which degrades its quality. This research utilized hyperspectral imaging technology to detect and visually analyze the soluble solid content (SSC) and firmness of the fruit, aiming to improve quality and achieve optimal pricing. Four maturity stages (color turning stage, coloring stage, maturity stage, and fully ripe stage) of Cerasus Humilis fruit were examined using hyperspectral images (895–1700 nm) alongside data collection on SSC and firmness. These samples were divided into a calibration set and a validation set with a ratio of 3:1 by sample set partitioning based on the joint X-Y distances (SPXY) method. The original spectral data was processed by a spectral preprocessing method. Multiple linear regression (MLR) and nonlinear least squares support vector machine (LS-SVM) detection models were established using feature wavelengths selected by the successive projections algorithm (SPA), competitive adaptive reweighted sampling (CARS), uninformative variable elimination (UVE), and two combined downscaling algorithms (UVE-SPA and UVE-CARS), respectively. For SSC and firmness detection, the best models were the SNV-SPA-LS-SVM model with 18 feature wavelengths and the original spectra-UVE-CARS-LS-SVM model with eight feature wavelengths, respectively. For SSC, the correlation coefficient of prediction (Rp) was 0.8526, the root mean square error of prediction (RMSEP) was 0.9703, and the residual prediction deviation (RPD) was 1.9017. For firmness, Rp was 0.7879, RMSEP was 1.1205, and RPD was 2.0221. Furthermore, the optimal model was employed to retrieve the distribution of SSC and firmness within Cerasus Humilis fruit. This retrieved information facilitated visual inspection, enabling a more intuitive and comprehensive assessment of SSC and firmness at each pixel level. These findings demonstrated the effectiveness of hyperspectral imaging technology for determining SSC and firmness in Cerasus Humilis fruit. This paves the way for online monitoring of fruit quality, ultimately facilitating timely harvesting. [ABSTRACT FROM AUTHOR]

Yellow pitahaya (Selenicereus megalanthus Haw.) is an exotic fruit with great potential for exportation in Ecuador. The research was carried out with the objective of evaluating the fruit growth and ripening as affected by four elicitors: salicylic acid (SA), methyl salicylate (MeSa), methyl jasmonate (MeJa), and oxalic acid (OA), all of them at 1, 5, and 10 mM concentration, compared with untreated plants (control). For each elicitor, nine plants were selected, and on each plant, three fruits were marked to follow up the growth by measuring polar and equatorial diameters. At harvest, yield (kg plant−1 and number of fruits plant−1), fruit weight, percentage of pulp and skin, total soluble solids (TSS), titratable acidity (TA), and firmness were determined. Treated plants enhanced fruit size, crop yield, and fruit weight compared with control fruits, although results depended on the elicitor tested and applied doses. The highest and lowest TSS were found in 10 mM MeSa and 5 mM MeJa-treated fruit, respectively, while the highest TA content was shown in 5 mM SA. Firmness was only enhanced in MeJa-treated fruits. Overall, results suggest that preharvest use of elicitors could modulate the pitahaya ripening and could improve quality attributes at harvest. [ABSTRACT FROM AUTHOR]

Flesh firmness in sweet cherries is determined using the measurement of normalized deformation force, i.e., determining the required force for a distance equal to 5 or 10% of the diameter of the cherries per millimeter. However, a firmness method involving a defined distance is quite simple and suitable for easy applications. Hence, our study focuses on the impact of fruit physiology under various and fixed distances. To assess the firmness evaluation, two sweet cherry cultivars (Canada Giant and Regina) were selected and subjected to three different levels of compression distance equal to 1%, 5%, 10% of the fruit's small thickness dimension along with a consistent compression distance of 0.16 mm. There was a strong correlation between panelists' preferences and the fruit that had been subjected to both a 1% deformation force and a fixed distance of 0.16 mm within each cultivar. Physiological traits, membrane integrity, and the metabolome of the fruit in these categories were mostly unaffected by the control (0%), or 1%, deformation force, as shown by clustering and PCA analysis. The control and 1% deformation force groups showed similar patterns, contrary to those of the 5% and 10% deformation force groups. Given these considerations, a fixed distance of 0.16 mm and a minimal 1% deformation force possess the potential to be employed and implemented for monitoring the firmness of sweet cherries during postharvest preservation. [ABSTRACT FROM AUTHOR]

Colostrum, the first milk produced by mammals, is rich in various bioactive components that provide numerous health benefits to newborns, such as growth factors, hormones, immunoglobulins, cytokines, and enzymes. Topical application of bovine or equine colostrum has been found to improve regeneration, accelerate cutaneous wound healing, and have moisturizing, protective, and anti-aging properties. The aim of this study was to examine the effect of a cosmetic preparation containing sheep colostrum on skin with signs of aging in mature women. Fifty-two women, aged 40–70, were randomized into two groups to receive either colostrum or placebo cream. The participants applied the cream for eight weeks. Skin hydration, TEWL, sebum, erythema, and tone were measured using a standardized Courage + Khazaka electronic GmbH Multi Probe Adapter; skin elasticity was measured with a cutometer, and images were taken by FotoMedicus. The treatment increased skin moisture, reduced TEWL, and improved skin firmness. These findings were confirmed by the subjective survey. The participants reported, inter alia, improved skin softness and less redness and hypersensitivity. Sheep colostrum cream was more effective at improving skin conditions than placebo cream. Colostrum creams can improve certain aspects of skin quality, especially the hydrolipid barrier, and overall rejuvenation. [ABSTRACT FROM AUTHOR]

Mangosteens can develop a postharvest physiological disorder, called "hardening", which affects their marketability and is not detectable using visual inspection. The hardening disorder of mangosteens was determined by firmness value using the texture analyzer. Near-infrared hyperspectral imaging (NIR-HSI) in the region of 935–1720 nm was tested as a possible rapid and non-destructive method to detect this disorder. The spectra from a region of interest of mangosteens were acquired and used for analysis. Calibration models for firmness of a similarly sized group and a mixed-size group were established using partial least squares regression (PLSR) and support vector machine regression (SVMR). Chemometric algorithms were investigated in order to determine the optimal conditions for establishing the models for firmness. The optimum model was obtained when the fruit were graded into similarly sized groups. Using partial least squares regression (PLSR), the correlation coefficient of prediction (Rp) was 0.87 and the root mean square error of prediction (RMSEP) was 6.25 N. The predictive images for firmness of the fruit were created by interpreting predicted firmness visualized as colors in every pixel. From the data, it was concluded that NIR-HSI can potentially be used to visualize hardening of individual mangosteens based on their predictive images. [ABSTRACT FROM AUTHOR]

Introduction: Goat milk has poorer fermentation characteristics due to the absence or only traces of αs1-casein, due to which goat yoghurt contains a less dense gel structure. Moreover, the fermentation characteristics of the milk vary between the breeds of the same species. Therefore, it becomes imperative to explore a few metabolites which could regulate the techno-functional properties of goat yoghurt. Objectives: This study was aimed at relating the metabolite profile of yoghurt prepared from milk of Barbari, an indigenous goat breed of India, and its techno-functional properties (firmness, whey syneresis, and flow behaviour) using multivariate data analysis and regression models. Results: Goat yoghurt was prepared with two different total solids (TS) levels (12 and 16%) and cultures, namely, commercial culture comprising a thermophilic yoghurt culture (A) and NCDC-263 comprising a mixed yoghurt culture (B). Results demonstrated a significant difference (p < 0.05) in whey syneresis with the increase in the TS level. Flow behaviour of all yoghurt samples showed a decrease in viscosity with an increase in shear rate, which confirmed its non-Newtonian behaviour and shear thinning nature, whereas frequency sweep confirmed its viscoelastic nature. Firmness was the most affected under the influence of different TS and culture levels. It was higher (p < 0.05) for 16-A, followed by 16-3B, and minimum for 12-2B. GC-MS-based metabolomics of the yoghurt revealed a total of 102 metabolites, out of which 15 metabolites were differentially expressed (p < 0.05), including 2-hydroxyethyl palmitate, alpha-mannobiose, and myo-inositol. Multivariate data analysis revealed clear separation among groups using principal component analysis and several correlations using a correlation heat map. Further, regression analysis exhibited methylamine (0.669) and myo-inositol (0.947) with higher regression coefficients (R2 values) exceeding 0.6, thus demonstrating their significant influence on the techno-functional properties, mainly firmness, of the yogurt. Conclusion: In conclusion, A gas chromatography-based metabolomics approach could successfully establish a relationship between the metabolome and the techno-functional properties of the yoghurt. [ABSTRACT FROM AUTHOR]

The market value of grapes (Vitis vinifera L.) is affected by their quality and harvesting time. Veraison and stage of ripening are the most important factors determining table grape quality. Therefore, the present research was performed to determine the effects of various ripening stages on the combination and postharvest quality of two red grape cultivars. Based on color change, fruits were harvested at the veraison stage, two weeks after the veraison stage, and at the full ripening stage. Fruits harvested at the fully ripe stage were stored for 7, 14, and 21 days (at 1 °C with 85–90% relative humidity). The greatest soluble solids content (SSC) (16.1%) and SSC/TA ratio were observed in 'Khoshnav', while the greatest titratable acidity (TA) (0.45%) and pH (3.60) were observed in 'Rashah'. The results of the present study showed that regardless of the storage period, fruits harvested at time V (veraison time) and two weeks after veraison (2WAV) had significantly higher firmness and vitamin C content. 'Khoshnav' had the lowest weight loss (2.05%), and 'Khoshnav' and 'Rashah' had the greatest firmness (5.95 N) and vitamin C content (89.48 mg 100 g−1 FW). The greatest anthocyanin content was observed on day 7 of storage in 'Rashah'. Total phenol (TP) and total flavonoid (TF) contents and antioxidant capacity (AC) increased significantly until harvest and decreased thereafter during cold storage. 'Rashah' showed the greatest AC, TP, and TF values. Based on the findings of this study, the 'Rashah' grape cultivar is shown to be rich in vitamin C, anthocyanins, flavonoids, phenolics, and antioxidants. It holds potential for utilization in both processing and breeding programs as a functional food ingredient. [ABSTRACT FROM AUTHOR]

The current study investigates the use of a non-destructive hyperspectral imaging approach for the evaluation of kiwifruit cv. "Hayward" internal quality, focusing on physiological traits such as soluble solid concentration (SSC), dry matter (DM), firmness, and tannins, widely used as quality attributes. Regression models, including partial least squares regression (PLSR), bagged trees (BTs), and three-layered neural network (TLNN), were employed for the estimation of the above-mentioned quality attributes. Experimental procedures involving the Specim IQ hyperspectral camera utilization and software were followed for data acquisition and analysis. The effectiveness of PLSR, bagged trees, and TLNN in predicting the firmness, SSC, DM, and tannins of kiwifruit was assessed via statistical metrics, including R squared (R²) values and the root mean square error (RMSE). The obtained results indicate varying degrees of efficiency for each model in predicting kiwifruit quality parameters. The study concludes that machine learning algorithms, especially neural networks, offer substantial accuracy, surpassing traditional methods for evaluating kiwifruit quality traits. Overall, the current study highlights the potential of such non-destructive techniques in revolutionizing quality assessment during postharvest by yielding rapid and reliable predictions regarding the critical quality attributes of fruits. [ABSTRACT FROM AUTHOR]

Research on silicon (Si), an element considered beneficial for plant growth, has focused on abiotic and biotic stress mitigation. However, the effect of Si on tomato fruit quality under normal growth conditions remains unclear. This study investigated the effects of applying different levels of Si (0 mmol·L−1 [CK], 0.6 mmol·L−1 [T1], 1.2 mmol·L−1 [T2], and 1.8 mmol·L−1 [T3]) in foliar sprays on tomato fruit quality cultivated in substrates, and the most beneficial Si level was found. Compared to CK, exogenous Si treatments had a positive influence on the appearance and nutritional quality of tomato fruits at the mature green, breaker, and red ripening stages. Of these, T2 treatment significantly increased peel firmness and single-fruit weight in tomato fruits. The contents of soluble sugars, soluble solids, soluble proteins, and vitamin C were significantly higher, and the nitrate content was significantly lower in the T2 treatment than in the CK treatment. Cluster analysis showed that T2 produced results that were significantly different from those of the CK, T1, and T3 treatments. During the red ripening stage, the a* values of fruits in the T2 treatment tomato were significantly higher than those in the other three treatments. Moreover, the lycopene and lutein contents of the T2 treatment increased by 12.90% and 17.14%, respectively, compared to CK. T2 treatment significantly upregulated the relative gene expression levels of the phytoene desaturase gene (PDS), the lycopene ε-cyclase gene (LCY-E), and the zeaxanthin cyclooxygenase gene (ZEP) in the carotenoid key genes. The total amino acid content in tomato fruits in the T2 treatment was also significantly higher than that of CK. In summary, foliar spraying of 1.2 mmol·L−1 exogenous Si was effective in improving the appearance and nutritional quality of tomato fruits under normal growth conditions. This study provides new approaches to further elucidate the application of exogenous silicon to improve tomato fruit quality under normal conditions. [ABSTRACT FROM AUTHOR]

γ-Aminobutyric acid (GABA) plays important roles in plant development, including the maintenance of fruit quality when applied as postharvest treatment. However, little information is available about the effects of preharvest GABA treatments. Thus, GABA (10, 50 and 100 mM) was applied as foliar spray at key points of fruit development in three sweet cherry cultivars and over two years. The results show that quality parameters, such as total soluble solid content, titratable acidity and firmness were higher in the fruit from GABA-treated trees than in the controls, either at harvest or during four weeks of cold storage. In addition, the total phenolic and total and individual anthocyanin concentrations were also enhanced by GABA treatments and the fruit color was improved. The activities of the antioxidant enzymes catalase, ascorbate peroxidase and peroxidase were also enhanced by the GABA treatments. The most effective concentration was 50 mM, which led to extending the storage period of sweet cherries with high quality traits to up to four weeks, while for the controls this was two weeks. Thus, GABA treatment had a clear effect on delaying the postharvest ripening and senescence processes in sweet cherries, with an additional effect on enhancing the content of bioactive compounds, such as phenolics and anthocyanins, with antioxidant properties and health benefits. [ABSTRACT FROM AUTHOR]

The effects of post-harvest spray treatments with essential oils (EOs) obtained from sage and aniseed on maintaining the quality of pears of the 'Williams' variety during storage was studied. Harvested pears were picked when they had reached their optimal maturity and underwent a treatment involving the application of aqueous solutions of glycerin, with varying amounts of sage essential oil (SEO) and aniseed essential oil (AEO). Weight loss during storage varied according to the treatment applied with the lowest values recorded for sage essential oil at concentrations of 300 ppm (6.24%) and 250 ppm (6.60%), respectively. Aniseed essential oil had a smaller effect on weight loss compared to sage essential oil. Fruit firmness was better maintained under the influence of the essential oil treatments, with those treated with sage essential oil standing out. The concentration of the essential oils that is used influences the antimicrobial activity of the post-harvest treatment that is applied, with higher essential oil concentrations leading to more pronounced decreases in the total number of mesophilic aerobic bacteria immediately after treatment (4.05 for SEO 200; 3.00 for SEO 300, respectively). The use of post-harvest techniques involving the application of aqueous solutions containing glycerol and essential plant oils by spraying can extend the shelf life of pear fruits. [ABSTRACT FROM AUTHOR]

Background: The firmness, weight, and size of blueberries are vital for commercial success of this crop. Fertilization is a key agronomic management practice that affects fruit quality, where calcium (Ca) plays a critical role. This study aimed to assess the impact of low levels of soil-dosed Ca in carboxylic acid form on fruit size, weight, firmness, and residual soil fertility. Methods: The study focused on two varieties of blueberries, Duke and Legacy, over two consecutive growing seasons on three commercial farms located in south-central Chile. This study consisted of five treatments, ranging from 0 to 4.0 kg Ca per hectare. Results: The highest firmness values observed for Duke were between 164 and 186 g mm−1, with size values ranging from 15.7 to 16.9 mm, and weight observations ranging from 1.60 to 1.76 g. On the other hand, Legacy showed firmness values between 163 and 173 g mm−1, with size values ranging from 16.2 to 17.2 mm, and weight observations ranging from 2.01 to 2.40 g. Conclusion: The application of low Ca rates to the soil did not impact the size, weight, or firmness of 'Duke' and 'Legacy' blueberries. There was a positive correlation between the Ca soil application and the concentration of exchangeable Ca. [ABSTRACT FROM AUTHOR]

In apple (Malus domestica), the level and timing of crop load have a major impact on the final fruit size and can also play a role in optimising internal fruit quality. Ideal crop loads vary with cultivar, but very few cultivars have recommended crop load targets that consider the effect of crop load on both return bloom and fruit quality. To address this issue, studies examining a range of crop loads and thinning times were undertaken on several apple cultivars. Return bloom and multiple fruit quality parameters were examined. The results of these studies demonstrate positive effects for early thinning, not only on fruit size but also on firmness and soluble solids content. Early-thinned fruit showed higher sugar levels than late-thinned fruit. Previously undemonstrated positive relationships between fruit sugar content and weight and between fruit firmness and weight in both 'Fuji' and 'Delicious', as well as between fruit sugar content and fruit firmness in 'Delicious', indicate that early thinning is a valuable tool in improving fruit quality. The current target crop load recommendations of 4–6 fruit cm−2 trunk cross-sectional area (TCSA) for 'Fuji' and 2–4 fruit cm−2 TCSA for 'Delicious' are confirmed by this study. New recommendations are proposed for the other cultivars in this study taking into account the impact of crop load on both fruit quality and return bloom. Both 'Pink Lady' and 'Gala' can support crop loads of up to eight fruit cm−2 TCSA without impacting return bloom, but fruit quality is compromised; hence, lower targets in the range of 4–6 fruit cm−2 TCSA are recommended. Large fruit size and good return bloom can be maintained in 'Jonagold' at crop loads of eight fruit cm−2 TCSA, while crop loads of four fruit cm−2 TCSA are suggested for 'Braeburn' to sustain regular bearing and good fruit size. [ABSTRACT FROM AUTHOR]

With the high production of apples in Poland, the priority actions include increasing their export volume. The main objective of the presented research was to maintain sufficient firmness in the apple cultivar Gala Schniga® SchniCo Red(s) transported to distant markets immediately after harvest or after long-term storage under ULO conditions (1.2% CO2 and 1.2% O2). In the study conducted during the 2021/2022 storage season on apples from the experimental orchard of the Warsaw University of Life Sciences (SGGW-WULS; Warsaw-52°14′ N, 21°1′ E), the effect of the pre- and post-harvest application of 1-MCP, harvest date, and simulated transport duration on the quality of apples in target retail trading conditions was evaluated. Apples collected on a given harvest date were divided into four samples: control (without the use of 1-MCP), Harvista™-sprayed, SmartFresh™-treated, and Harvista™-sprayed + SmartFresh™-treated. Immediately after harvest and after 9 months of storage, the apples were packed in boxes and stored at 1 °C for 6 and 8 weeks (simulated transport conditions). Directly after the simulated transport and after an additional 7 and 14 days at 25 °C (handling conditions in hot countries), the following parameters were determined: firmness, SSC, TA, and ethylene production. The study noted a significant effect of the use of 1-MCP, harvest time, and simulated transport period on all tested parameters. Apples from trees sprayed with Harvista™ maintained a firmness of >55 N for 14 days of shelf-life only if they were harvested at the optimal date and transported immediately after harvest, and if their shipping lasted 6 weeks. Such firmness could be preserved after 8 weeks of the transport of SmartFresh™-treated apples harvested at the optimal date, and of Harvista™-sprayed + SmartFresh™-treated apples harvested at a delayed date. In the latter combination, apples also maintained the desired firmness after 9 months of storage + 6 weeks of transport regardless of the harvest date. [ABSTRACT FROM AUTHOR]

The kiwifruit (Actinidia deliciosa cv. 'Hayward'), which shows climacteric characteristics, continues to ripen after harvest. In this process, quality losses occur in fruits, and this causes economic losses. The post-harvest storage conditions are essential in preventing these losses. The main purpose of this study was to assess the effect of agro-ecological conditions on quality traits and bioactive compounds of the kiwifruit throughout the shelf life. In this study, kiwifruit grown in 5 different locations (Ordu, Giresun, Rize, Samsun, and Yalova) constituted the plant material of the study. The fruits treated with modified atmosphere packaging (MAP) were stored at 0 ± 0.5 °C and 90 ± 5% relative humidity (RH) for 150 days in cold storage. For shelf life measurements, fruits were kept at 20 ± 1 °C and 65 ± 5% RH for 5 d. Quality analysis was performed at monthly intervals (at harvest, 30th, 60th, 90th, 120th, and 150th d). In this study, the lowest respiration rate at the end of the shelf life was measured in kiwifruit grown in Yalova. Flesh firmness was higher in kiwifruit grown in Rize, Ordu, and Giresun than Samsun. In all periods, Yalova's L* value in flesh was higher than that of Ordu. The opposite situation was observed for the hue angle. The soluble solids content (SSC) values measured in Samsun and Yalova were higher than in Ordu and Rize. In the last four measurements, higher vitamin C was detected in Yalova than in Ordu, Rize and Giresun. In all periods, the highest total phenolics and antioxidant activity (in DPPH assay) were obtained in kiwifruit grown in Yalova. On the contrary, the highest flavonoids were measured in kiwifruit grown in Ordu. As a result, it was revealed that agro-ecological conditions may affect the quality traits and bioactive compounds of kiwifruit. [ABSTRACT FROM AUTHOR]

Cold quarantine storage is the practice of subjecting citrus fruit to low temperatures after harvesting to comply with stringent international phytosanitary standards for export, but fruit quality can be affected during storage. Therefore, this study investigated the effects of glycine betaine (GB) and/or methyl salicylate (MeSA) on physicochemical changes, chemical attributes of juice, and peel color of 'Moro' blood orange at cold quarantine storage (2 °C) for 60 days. Fruit were treated with GB (15 and 30 mM) by vacuum infiltration at 30 kPa for 8 min and vapor treatment of MeSA (100 µM) for 18 h as well as the combination of both GB concentrations with MeSA. The key findings of this research revealed that the combined treatment of 30 mM GB and 100 µM MeSA significantly mitigated weight and firmness losses in 'Moro' blood orange fruit during the cold quarantine period. Furthermore, there was a decrease in titratable acidity (TA) across all treatments, with the highest TA recorded for the 30 mM GB + 100 µM MeSA combination. Conversely, total soluble solids (TSS), TSS/TA ratio, and juice pH increased in all treatments, with the control treatment displaying the highest values. Regarding peel color parameters, which encompass L* (lightness), b*, hue angle (h°), chroma (C*), and a*, as well as the citrus color index (CCI), these exhibited characteristic changes during cold quarantine storage. However, the application of GB and MeSA, especially at the 30 mM GB + 100 µM MeSA level, noticeably delayed these peel color variations. Overall, GB and MeSA treatments offer significant advantages in preserving the physicochemical characteristics and chemical attributes of 'Moro' blood oranges during cold quarantine storage. These findings underscore the potential of GB and MeSA treatments for maintaining the quality of 'Moro' blood oranges during cold quarantine storage, with a noteworthy synergistic effect between MeSA and GB in preserving fruit quality. [ABSTRACT FROM AUTHOR]

Pomegranate is one of the most important and widely distributed trees. Boron and zinc are important nutrients for plant growth and fruit quality. Nanotechnology has emerged as one of the most innovative scientific fields in agriculture. This study was conducted to describe the changes in the physiochemical characteristics (weight, diameter, length, firmness and color), as well as the phytochemicals attributes (total phenolics, total flavonoids, ascorbic acid, anthocyanin and antioxidant %) and minerals contents, of pomegranates fruits of the 'Wonderful' cultivar as a result of spraying pomegranate trees using nanomaterials (zinc oxide (ZnONPs) and boron oxide (B2O3NPs)). In three successive developmental stages (full bloom, 6 weeks after full bloom and one month before harvest time), the trees were sprayed with 0.25, 0.5 and 1 g/L ZnONPs, as well as 0.25, 0.5 and 1 g/L B2O3NPs during the 2021 and 2022 seasons. The application of ZnONPs and B2O3NPs influenced the qualitative characteristics of the fruits in the studied seasons. The highest marketable % was observed for the 0.50 and 1 g/L ZnONPs and 1 g/L B2O3NPs compared to the other treatments. Also, a positive effect was recorded for the ZnONPs and B2O3NPs on the fruits' physical properties. All of the ZnONP and B2O3NP treatments resulted in increasing the total phenolic, flavonoid, anthocyanin and ascorbic acid contents and the antioxidant activity in the pomegranate juices. In conclusion, our results suggest that spraying pomegranate trees with ZnONPs and B2O3NPs improves the marketable fruit, enhances the fruit quality and increases the bioactive components and antioxidant activity. [ABSTRACT FROM AUTHOR]

The ground has long been cited as a key contributing factor for injury risk in the cross-country phase of eventing. The current study aimed to develop a practically useful standardized protocol for measuring eventing cross country ground. Data collection was split into three phases: Phase 1 (Validation), Phase 2 (Expansion of data set), and Phase 3 (Threshold establishment). During Phase 1, data from nine event courses were collected using an Orono Biomechanical Surface Tester (OBST), Vienna Surface Tester (VST), Lang Penetrometer, Going Stick, and moisture meter. Using linear regression, 80% of the variability in cushioning measured with the OBST was predicted from moisture and VST measurements (p < 0.001). In Phase 2, objective data from 81 event courses and subjective assessments from 180 event riders were collected. In Phase 3, k-means cluster analysis was used to classify the courses into ten clusters based on average course measurements of moisture, cushioning, firmness, stiffness, depth, and coefficient of restitution. Based on cluster membership, course average subjective data (16 courses) were compared using a General Linear Model. Significant differences (p < 0.05) in subjective impact firmness (p = 0.038) and subjective cushioning (p = 0.010) were found between clusters. These data and cluster thresholds provide an event course baseline for future comparisons. [ABSTRACT FROM AUTHOR]

White-fruited strawberry cultivars have recently become popular due to their exotic appearance and flavor, but more needs to be known about their overall quality and postharvest performance. The objective of this study was to characterize and compare the overall quality of the white-fruited strawberry Florida Pearl® 'FL 16.78-109' against the commercial, red-fruited strawberry 'Florida Brilliance' at harvest and during cold storage (1 °C). Results showed that harvest date and weather conditions contributed to significant differences in fruit quality, regardless of the cultivar. However, Pearl was softer at harvest and had lower total phenolic and anthocyanin contents but was less acidic and had higher total sugars and ascorbic acid contents than Brilliance. Pearl major polyphenols were kaempferol 3-glucoside, quercetin 3-glucoside, quercetin, and gallic acid, while for Brilliance epicatechin, pelargonidin, pelargonidin 3-glucoside, and ferulic acid were the major polyphenol compounds identified. After cold storage, Pearl lost less weight than Brilliance and showed a less dramatic decline in individual polyphenols. Pearl and Brilliance anthocyanins and phenolic acids were the polyphenol groups most affected by cold storage because they showed the highest decline from harvest to the end of storage. Cold storage also had different effects on other polyphenols, but the effect was cultivar-dependent. Overall, white strawberries have a unique appearance, are sweet, have an excellent bioactive profile, and can maintain good postharvest quality. [ABSTRACT FROM AUTHOR]

An important problem of container nurseries is ensuring equal and favorable growth conditions for cultivated plants. This can be achieved by ensuring the physical parameters of the substrate used to grow seedlings in individual cells of the container are similar. The nursery container is filled with a specially composed substrate through an automated line. Quickly controlling the parameters related to the quality of substrate filling presents a significant problem, as it requires the ongoing correction of the filling module settings (e.g., extending the vibration time or changing the vibration amplitude). To address this issue, it would be helpful to determine the compactness of the substrate, which can be easily measured using a penetrometer. This paper presents a prototype automated station, known as a multipenetrometer, designed for the simultaneous testing of compactness in 15 selected container cells. The prototype was put to the test at the Nursery Farm in Sukowo, where two types of polystyrene containers (V150—650/312/150 mm; 74 cells; and 0,148 cm3 cell volume and V300—650/312/180 mm; 53 cells; and 0.275 cm3 cell volume) were filled with peat–perlite substrate on the Urbinati Ypsilon automated line. This study investigated the influence of substrate moisture (two levels—70 and 75%) and vibration intensity (two levels—8 and 12 G) of the vibrating table on its compactness within the individual cells of the nursery container. The results indicated that with an increase in substrate moisture and vibration intensity, the compactness of the substrate increased, and the variation in compactness between individual cells decreased. Notably, the V300 containers, with a larger cell volume (265 cm3), experienced a higher level of change compared to the V150 containers (145 cm3). Despite the use of substrate compaction techniques based on the experience of line operators filling containers, the coefficient of variation between the compactness of the substrate in individual cells of the container remained at 30%. Based on the findings, it was confirmed that the optimal parameters for filling V150 and V300 containers with peat–perlite substrate on the Urbinati line, at a filling capacity of approximately 400 containers h−1, are a moisture content of around 75% and a maximum vibration intensity of 12 G. [ABSTRACT FROM AUTHOR]

Increasingly, in orchards around the world that are planted one after another, disturbances are observed, and these issues with growth and development are called replantation disease. It is manifested mainly by poor tree growth after planting and poor ripening. One way to reduce replantation disease is to improve soil fertility after many years of fruit tree cultivation. The aim of the work was to evaluate the growth and yield of cherries after replantation and to compare this with a site where fruit trees had not grown before. The trees were planted at two sites: after the replantation of the cherry orchard (OR1) and in a site where fruit trees had not been cultivated before (OR2). Two combinations were used in each orchard: boiler without mulching (C), mulch—after planting mulching with a substrate after growing mushrooms (M). The trees at the site after replantation grew and bore less fruit than in the position where fruit trees had not grown before. The disease also affected some of the quality characteristics of the fruit. This resulted in an increase in fruit weight and a darker color (L*) and a higher value of hue fruit color. Mulching, which is often recommended in orchards planted after previous cultivation, did not provide the expected improvement. It did not significantly affect tree growth and yield. Only an effect on the content of components in the soil was observed, but it affected the condition of the trees. In addition, we analyzed how experimental combinations responded to climatic conditions by calculating the correlations between the SAT (sum of active temperatures) and the stages of tree development. [ABSTRACT FROM AUTHOR]

Spain is a great producer of organic lemon; however, it is necessary to reduce the losses caused by post-harvest diseases. Melatonin (MEL) is a naturally occurring compound with physiological functions in fruit growth and ripening and is able to modulate postharvest ripening and senescence, most of it being concentrated in climacteric fruit. Thus, the aim of this study was to apply MEL to organic lemon fruit with stems and leaves (LEAF) and to organic lemon without those components (LEAFLESS) after harvesting and storage during 21 days at 2 °C to understand the effects of this treatment on the fruit quality. For this purpose, two experiments were carried out. First, MEL was applied at 0.01 mM, 0.1 mM and 1.0 mM by immersion for 15 min on lemon fruits, and the quality parameters and bioactive compounds of the fruit were analysed. Subsequently, a second experiment was carried out where the best concentration (1 mM) was selected and another time (15 and 30 min) was added, with the same quality parameters being analysed. As a result, we observed that all MEL treatments showed positive effects on weight loss reduction, softening (higher fruit firmness), total acidity and lower colour changes. Total phenols increased in MEL-treated lemons, both in peel and juice. For the three concentrations tested, the best efficiency was obtained with MEL at 1.0 mM, while LEAF lemons were the most effective. In conclusion, lemons containing stems and leaves (LEAF) improved preservability by using MEL at 1.0 mM with better organoleptic quality and enhanced phenolic compounds. [ABSTRACT FROM AUTHOR]

The use of plant-based compost has been increasing within environmentally sustainable crop systems, as its incorporation into soil improves its structure and implies a slow release of nutrients to the plants. Due to the limited literature regarding compost application to industrial crops and the important role of the soil type, research was conducted on the industrial tomato (Solanum lycopersicum L.) hybrid Coronel F1 at the Department of Agricultural Sciences of Naples, University Federico II, in 2019 and 2020. The study was based on the factorial combination of three fertilization types (compost, compost + mineral, and mineral) and three soil textures (clayey, loamy, and sandy). The highest crop yield was observed in loamy soil with mineral fertilization (+12.7% compared to clayey and loamy soils; +12.1% and +60.3% compared to compost + mineral and compost, respectively). Compost application increased plant dry weight (+23% compared to mineral fertilization), while sandy soil had a lower dry residue (−3%). The combination of loamy soil and compost exhibited the highest fruit dry matter percentage (approximately 7%). These findings suggest that applying compost to industrial tomato plants, alone or with mineral fertilizers, improves fruit quality and promotes crop system sustainability, and the optimal strategy depends on the target crop and soil type. [ABSTRACT FROM AUTHOR]

Humic acids are plant biostimulants, which can be used in horticulture as an effective and relatively inexpensive alternative to chemical means of production. The aim of the study was to assess how the fertiliser containing humic acids affected the growth, yield, and quality of strawberry fruits. In 2021, an experiment was conducted on two-year-old strawberry bushes (Fragaria× ananassa (Duchesne ex Weston) Duchesne ex Rozier) of the 'Rumba' cultivar growing on a horticultural farm in northern Wielkopolska, Poland. During the growing season, the soil was fertilized by the mineral fertilization and the bushes were sprayed two, three, or four times with the Humi Brown Gold fertiliser containing humic acids. In the experiment, the soil enzymatic activity, biometric parameters of strawberry leaves, fruit yield and fruit quality was assessed. It was determined that, in the experimental treatments where foliar fertilisation had been applied, the activities of proteases and dehydrogenases as well as soil respiration increased by more than double and were significantly higher than in the variants where soil fertilisers had been applied. The strawberry bushes treated with the humic acids fertiliser developed more than 60% larger surface leaves, bloomed more intensively and gave a higher yield. Fruits with significantly higher firmness were harvested from such bushes, with higher weight and extract content than those where the soil fertiliser had been applied. The difference was several tens of percent. This leads to the conclusion that the foliar application of humic acids could be an effective alternative to mineral fertilisation in strawberry plantations. [ABSTRACT FROM AUTHOR]

Changing the harvesting time of apples from the optimum harvest window to delayed harvesting may be applied if it is impossible to harvest apples at the optimal time, but it requires changing other factors, as they influence the quality of fruits and shelf life. The aim of the study was to assess the quality of 'Red Jonaprince' apples during storage after delayed harvesting and 1-methylcyclopropene (1-MCP) preharvest and postharvest treatment for various storage times. Apples were studied within four groups subjected to preharvest and postharvest treatments, as follows: Group 0—no 1-MCP treatment; Group 1—1-MCP preharvest treatment; Group 2—1-MCP postharvest treatment; and Group 3—1-MCP preharvest and postharvest treatment. All apples were subjected to ultra-low oxygen (ULO) storage conducted for 3, 5 or 6 months, while the analyses were conducted directly after ULO storage (simulated shelf life—0 days) and after simulated shelf life (7 days). For firmness, in the case of 1-MCP applied only preharvest (Group 1) and only postharvest (Group 2), before shelf life, the longer ULO storage resulted in obtaining lower values of firmness (p < 0.0001). If 1-MCP was not applied postharvest (Group 0 and Group 1), and short ULO storage was applied (3 and 5 months for Group 0; 3 months for Group 1), after shelf-life lower values of firmness were observed (p < 0.0001). For soluble solids content (SSC), in the case of 1-MCP not applied preharvest (Group 0 and Group 2), before shelf life, and for 1-MCP applied postharvest (Group 2) after shelf life, the longer ULO storage resulted in obtaining lower values of SCC (p < 0.0001). For titratable acidity (TA), in the case of all the studied groups after shelf life, as well as in case of 1-MCP applied only preharvest (Group 1) also before shelf life, the longer ULO storage resulted in obtaining lower values of TA (p < 0.0001). Except for the 1-MCP applied only postharvest (Group 2), in the case of short ULO storage applied (3 and 5 months for Group 0; 5 months for Group 1; 5 months for Group 3), after shelf-life lower values of TA were observed (p < 0.0001). If delayed harvesting must be conducted, applying 1-MCP not only postharvest, but also preharvest, allows obtaining the most stable firmness and SSC, which do not decrease during storage and shelf life. Taking this into account, it may be concluded, that in the case of delayed harvesting, combining 1-MCP applied preharvest and postharvest should be recommended to keep the quality parameters stable during storage and shelf life. [ABSTRACT FROM AUTHOR]

γ-aminobutyric acid (GABA) is a naturally occurring plant compound that acts as a signalling agent during stress conditions, mainly due to unstable events, although sometimes the endogenous content does not reach sufficient levels. Thus, the main aim of this study was to apply GABA preharvest treatments in lemon and to study its effects on quality attributes at harvest and during postharvest storage. GABA was applied as foliar spray at 10, 50, and 100 mM, and quality traits during 28 days of storage at two temperatures (at 2 and 10 °C) were determined. Results show that all GABA treatments had a positive effect on reducing the weight losses and fruit softening. In addition, crop yield in terms of kg tree−1 and fruit number tree−1 was improved for the first and second harvest as well as the total phenolics content and total antioxidant activity (TAA). In conclusion, GABA at 50 mM concentration was the most effective preharvest treatment, enhancing shelf life being enhanced for 14 and 7 days at 2 and 10 °C, respectively, with respect to control lemons. [ABSTRACT FROM AUTHOR]

Blueberries (Vaccinium corymbosum L.) are becoming increasingly popular for their nutritional and health benefits, and their economic value is therefore increasing. The loss of quality that can occur due to softening and fungal attack is an important consideration when marketing blueberries. Despite the added value of blueberries, no studies have been carried out on how the fruit arrives at the outlets just before purchase by the consumer in terms of firmness, physico-chemical parameters, phenolic compounds, and fungal growth. The aim of this work has been, therefore, to investigate possible differences in quality parameters between blueberries purchased from ten different outlets, regardless of the supplier. The results showed that all the samples were of acceptable quality, although they all had a low maturity index at the point of sale. None of the samples studied showed clear signs of fungal decay at the time of purchase, although we were able to grow and identify some pathogen specimens after cultivation. In terms of total phenolic and anthocyanin content, as well as antioxidant activity, all the samples showed low values, possibly due to their postharvest storage, but they were within the expected range for this fruit. On the other hand, differences in the measured parameters were observed between samples of the same cultivar while no differences were found between conventionally and organically grown blueberries. This suggests that preharvest (such as edaphoclimatic conditions, agricultural practices, and cultivars) and postharvest factors (such as treatments used, storage, and transport temperatures) could influence the berry quality when they reach the consumer. [ABSTRACT FROM AUTHOR]

The firmness of the flesh fruit is a very important feature in the eating process. Peach fruit is very hard during development, but its firmness slightly decreases in the later stages of development. While there has been extensive research on changes in cell wall polysaccharides during fruit ripening, little is known about the changes that occur during growth and development. In this study, we investigated the modifications in cell wall components throughout the development and ripening of peach fruit, as well as its impact on firmness. Our findings revealed a significant positive correlation between fruit firmness and cellulose content at development stage. However, the correlation was lost during the softening process, suggesting that cellulose might be responsible for the fruit firmness during development. Members of the chitinase-like protein (CTL) group are of interest because of their possible role in plant cell wall biosynthesis. Here, two CTL homologous genes, PpCTL1 and PpCTL2, were identified in peach. Spatial and temporal expression patterns of PpCTLs revealed that PpCTL1 exhibited high expression abundance in the fruit and followed a similar trend to cellulose during fruit growth. Furthermore, silencing PpCTL1 expression resulted in reduced cellulose content at 5 DAI (days after injection), this change that would have a negative effect on fruit firmness. Our results indicate that PpCTL1 plays an important role in cellulose biosynthesis and the maintenance of peach firmness during development. [ABSTRACT FROM AUTHOR]

Plums are primarily marketed for fresh consumption, canning, freezing, jam and jelly. Unfortunately, plum consumption has remained steady or declined. Consumers complain about a lack of flavor quality but are willing to pay for higher quality. Thus, absence of flavor and cold storage disorders are the main barriers to consumption. Plum cultivars are sensitive to gel breakdown, flesh browning and 'off flavors'. Consumer approval and postharvest life are controlled by genotype, quality attributes, harvest date and proper postharvest handling. A consumer quality index (CQI) based on soluble solids concentration (SSC) and minimum firmness is proposed to maximize flavor and postharvest life. In most cases, late harvest increases quality attributes. Our work and industry experience demonstrated that using critical bruising thresholds (CBT) based on minimum firmness measured at harvest acts as a reliable predictor of how late to harvest safely for maximum visual and sensory quality. Plums tolerated late harvest well because of their high tolerance to bruising damage, but suitable postharvest temperature management and selling within the potential postharvest life are required to maintain flavor and avoid the beginning of chilling injury. Thus, to maximize flavor and postharvest life, a CQI based on SSC and minimum firmness measured at consumption is proposed. This article provides guidance on using this CQI, combined with proper postharvest handling techniques, such as correct harvest date determination and temperature management, to maintain quality and increase consumption. [ABSTRACT FROM AUTHOR]

It is widely recognized that the quality of fruits and vegetables can be altered during transportation and storage. Firmness and loss of weight are the crucial attributes used to evaluate the quality of various fruits, as many other quality attributes are related to these two attributes. These properties are influenced by the surrounding environment and preservation conditions. Limited research has been conducted to accurately predict the quality attributes during transport and storage as a function of storage conditions. In this research, extensive experimental investigations have been conducted on the changes in quality attributes of four fresh apple cultivars (Granny Smith, Royal Gala, Pink Lady, and Red Delicious) during transportation and storage. The study evaluated the weight loss and change in firmness of these apples varieties at different cooling temperatures ranging from 2 °C to 8 °C to assess the impact of storing at these temperatures on the quality attributes. The results indicate that the firmness of each cultivar continuously decreased over time, with the R2 values ranging from 0.9489–0.8691 for red delicious, 0.9871–0.9129 for royal gala, 0.9972–0.9647 for pink lady, and 0.9964–0.9484 for granny smith. The rate of weight loss followed an increasing trend with time, and the high R2 values indicate a strong correlation. The degradation of quality was evident in all four cultivars, with temperature having a significant impact on firmness. The decline in firmness was found to be minimal at 2 °C, but increased as the storage temperature increased. The loss of firmness also varied among the four cultivars. For instance, when stored at 2 °C, the firmness of pink lady decreased from an initial value of 8.69 kg·cm2 to 7.89 kg·cm2 in 48 h, while the firmness of the same cultivar decreased from 7.86 kg·cm2 to 6.81 kg·cm2 after the same duration of storage. Based on the experimental results, a multiple regression quality prediction model was developed as a function of temperature and time. The proposed models were validated using a new set of experimental data. The correlation between the predicted and experimental values was found to be excellent. The linear regression equation yielded an R2 value of 0.9544, indicating a high degree of accuracy. The model can assist stakeholders in the fruit and fresh produce industry in anticipating quality changes at different storage stages based on the storage conditions. [ABSTRACT FROM AUTHOR]

GABA (γ-Aminobutyric acid) is an amino acid with diverse effects on plant development, including delayed senescence in horticultural commodities. The main goal of this research was to investigate the impact of GABA applications to pomegranate trees on the 'Mollar de Elche' quality traits and antioxidant compounds during storage for 60 days at 10 °C. In the 2019 experiment, pomegranate trees received foliar sprays of GABA at 10-, 50- or 100-mM concentrations at three stages of fruit development. Pomegranates harvested from GABA-treated trees exhibited enhanced fruit firmness, titratable acidity, the red color of skin and aril, and higher levels of total phenolics and total and specific anthocyanins compared to those obtained from the control group. These quality traits were maintained at higher levels during 60 days of preservation at 10 °C, and the most important effects were observed for 100 mM dose. In a 2020 experiment, GABA was sprayed at a 100 mM dose, and results were confirmed, showing a retard of the ripening process in pomegranates from GABA-treated trees concerning controls for two months of storage and an extension of their shelf life (with optimal quality traits appreciated by consumers) from 30 to 60 days. Moreover, the increase in anthocyanin content due to GABA treatments resulted in redder fruits, which would appeal more to consumers and have improved health benefits. [ABSTRACT FROM AUTHOR]

Biostimulants help plants cope with environmental stresses and improve vegetable yield and quality. This study was conducted to determine the protein hydrolysate (PH) effect of three different durations (weekly applications: three, six, or nine times plus an untreated control) in factorial combination with four soil electrical conductivities (EC: 1.5, 3.0, 4.5, or 6.0 mS·cm−1) on yield, fruit quality, and elemental composition of tomato 'miniplum' grown in a greenhouse. Fruit yield was best affected, during the summer, by six and nine biostimulant applications at EC 3.0 mS·cm−1, and in the same season, the six treatments led to the highest fruit number with no difference compared to nine applications; during the winter, the three and six treatments improved the mentioned variables at each EC level. Fruits' dry residue and Brixo were positively affected by biostimulation both in summer and winter. In summer, the 6.0 mS·cm−1 EC led to the highest dry residue and Brixo values, though the latter did not show any significant difference compared to 4.5 mS·cm−1; in winter, the best results corresponded to 4.5 and 6.0 mS·cm−1. A higher beneficial effect of PH on fruit antioxidant status, i.e., lycopene, polyphenols, ascorbic acid levels, and lipophilic (LAA) and hydrophilic (HAA) activity, was recorded in winter compared with summer. Positive correlations between polyphenols and LAA, as well as ascorbic acid content and HAA were found for all EC and PH treatments. Most of the mineral elements tested demonstrated concentration stability, whereas the highest EC decreased P, Mg, Cu, and Se accumulation. The opposite effect was shown by PH application on Se and Mn levels, with P tending to increase. The concentrations of Fe, Zn, and Cu were the lowest under the longest duration of PH supply. These results further confirm the essential role of plant biostimulation in enhancing tomato yield and quality, with a particular focus on the treatment duration. [ABSTRACT FROM AUTHOR]

This study evaluated the physiochemical changes of one commercial European pear variety 'Conference' and one Asian pear variety 'Yali' during 60 days of storage at 1 °C. Content of organic acids, ethylene and formation of CO2 were determined using HPLC/UV-VIS and GC/FID and TCD detectors, respectively. This study presents an evaluation of the influence of the above-mentioned factors and long-term storage on quality parameters, including the antioxidant capacity of an important European and Asian pear cultivar. There was a significant effect of temperature on respiration rate in both varieties. Development of the respiration intensity had a similar course in European and Asian pears. The high values of CO2 production at the beginning of storage for the variety 'Conference' (14.08 mg·kg−1·h−1) and 'Yali' (23.37 mg·kg−1·h−1) were followed by a sharp decline of 80% and 83% at later stages of storage at 1 °C, respectively. Ethylene formation in fruit increased with storage time and was the highest after 60 days in cold storage, especially in 'Yali' (7.14 µL·kg−1·h−1). The results show the relation between ethylene formation and ripening-related fruit parameters. The 'Yali' variety showed negligible changes in soluble solids content and flesh firmness during storage ranging from 0.35 to 0.60 MPa. The most represented sugar determined enzymatically was fructose and malic acid was the dominant organic acid in pear fruit. Antioxidant activity determined using the FRAP and DPPH methods did not change significantly during 60 days of storage. [ABSTRACT FROM AUTHOR]

'Lemonato' is a Greek peach melting-flesh white-flesh cultivar with high nutritional value highly appreciated by the consumers. This study aimed to evaluate the effect of pre-harvest foliar calcium application on fruit quality, primary metabolite profile, antioxidant activity, total phenolic content, and phenolic profile of the 'Lemonato' peach, clone 'Stamatis'. The experiment was conducted for two years, 2019 and 2020, in two commercial orchards at Kato Lehonia and Agios Vlasios regions, central Greece, where the 'Lemonato' clone 'Stamatis' is traditionally cultivated. The treatments were organic calcium (Ca), calcium–silicate in nanoparticles (Ca–Si), and calcium chloride (CaCl2). Foliar application of the different Ca formulations, commonly used as a horticultural practice, were not effective at improving the fruit quality characteristics in this clone, which is characterized by fruit softening during ripening. The study revealed the sugars and organic acid composition and phenolic profile of the 'Lemonato' peach, clone 'Stamatis'. Peach fruit quality, primary metabolites, and phenolic compounds of the two orchards showed a different response to organic Ca and Ca–Si, indicating that genetic or environmental factors may also be involved. A higher concentration of organic Ca and CaCl2 increased the peach fruit phenolic compounds content and the total antioxidant activity, improving the fruit nutritional quality. [ABSTRACT FROM AUTHOR]

Spain is the world's leading producer of cherimoya, a climacteric fruit highly appreciated by consumers. However, this fruit species is very sensitive to chilling injury (CI), which limits its storage. In the present experiments, the effects of melatonin applied as dipping treatment on cherimoya fruit CI, postharvest ripening and quality properties were evaluated during storage at 7 °C + 2 days at 20 °C. The results showed that melatonin treatments (0.01, 0.05, 0.1 mM) delayed CI, ion leakage, chlorophyll losses and the increases in total phenolic content and hydrophilic and lipophilic antioxidant activities in cherimoya peel for 2 weeks with respect to controls. In addition, the increases in total soluble solids and titratable acidity in flesh tissue were also delayed in melatonin-treated fruit, and there was also reduced firmness loss compared with the control, the highest effects being found for the 0.05 mM dose. This treatment led to maintenance of fruit quality traits and to increases in the storage time up to 21 days, 14 days more than the control fruit. Thus, melatonin treatment, especially at 0.05 mM concentration, could be a useful tool to decrease CI damage in cherimoya fruit, with additional effects on retarding postharvest ripening and senescence processes and on maintaining quality parameters. These effects were attributed to a delay in the climacteric ethylene production, which was delayed for 1, 2 and 3 weeks for 0.01, 0.1 and 0.05 mM doses, respectively. However, the effects of melatonin on gene expression and the activity of the enzymes involved in ethylene production deserves further research. [ABSTRACT FROM AUTHOR]

Proanthocyanidins (PAs), also known as condensed tannins, are widespread throughout the plant kingdom, presenting diverse biological and biochemical activities. Being one of the most abundant groups of natural polyphenolic antioxidant, PAs are applied to improve plant tolerance to (a)biotic stresses and delay the senescence of fruit by scavenging the reactive oxygen species (ROS) and enhancing antioxidant responses. The effects of PAs on coloring and softening of strawberries (Fragaria × ananassa Duch.), a worldwide demanded edible fruit and typical material for studying non-climacteric fruit ripening, were firstly assessed in this work. The results showed that exogenous PAs delayed the decrease in fruit firmness and anthocyanins accumulation but improved the fruit skin brightness. Strawberries treated with PAs had similar total soluble solids, total phenolics, and total flavonoids, but lower titratable acidity content. Moreover, the contents of endogenous PAs, abscisic acid and sucrose, were somehow increased by PA treatment, while no obvious change was found in fructose and glucose content. In addition, the anthocyanin- and firmness-related genes were significantly repressed, while the PA biosynthetic gene (anthocyanin reductase, ANR) was highly up-regulated by PA treatment at the key point for fruit softening and coloring. In summary, the results presented in this study suggest that PAs slow down strawberry coloration and softening by inhibiting the expression of related genes, which could be helpful for a better understanding of the biological role of PAs and provide a new strategy to regulate strawberry ripening. [ABSTRACT FROM AUTHOR]