1. Effects of Mini-Dystrophin on Dystrophin-Deficient, Human Skeletal Muscle-Derived Cells.
- Author
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Meng J, Counsell J, and Morgan JE
- Subjects
- Cell Differentiation, Cell Engineering methods, Cell Proliferation, Dystrophin antagonists & inhibitors, Dystrophin metabolism, Gene Expression Regulation, Genes, Reporter, Genetic Vectors chemistry, Genetic Vectors metabolism, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Humans, Lentivirus genetics, Lentivirus metabolism, MAP Kinase Signaling System, Muscle Fibers, Skeletal pathology, Muscle, Skeletal pathology, Muscular Dystrophy, Duchenne metabolism, Muscular Dystrophy, Duchenne pathology, Plasmids chemistry, Plasmids metabolism, Primary Cell Culture, RNA, Small Interfering genetics, RNA, Small Interfering metabolism, Spectrin genetics, Spectrin metabolism, Transduction, Genetic, Dystrophin genetics, Muscle Fibers, Skeletal metabolism, Muscle, Skeletal metabolism, Muscular Dystrophy, Duchenne genetics, Transgenes
- Abstract
Background: We are developing a novel therapy for Duchenne muscular dystrophy (DMD), involving the transplantation of autologous, skeletal muscle-derived stem cells that have been genetically corrected to express dystrophin. Dystrophin is normally expressed in activated satellite cells and in differentiated muscle fibres. However, in past preclinical validation studies, dystrophin transgenes have generally been driven by constitutive promoters that would be active at every stage of the myogenic differentiation process, including in proliferating muscle stem cells. It is not known whether artificial dystrophin expression would affect the properties of these cells., Aims: Our aims are to determine if mini-dystrophin expression affects the proliferation or myogenic differentiation of DMD skeletal muscle-derived cells., Methods: Skeletal muscle-derived cells from a DMD patient were transduced with lentivirus coding for mini-dystrophins (R3-R13 spectrin-like repeats (ΔR3R13) or hinge2 to spectrin-like repeats R23 (ΔH2R23)) with EGFP (enhanced green fluorescence protein) fused to the C-terminus, driven by a constitutive promoter, spleen focus-forming virus (SFFV). Transduced cells were purified on the basis of GFP expression. Their proliferation and myogenic differentiation were quantified by ethynyl deoxyuridine (EdU) incorporation and fusion index. Furthermore, dystrophin small interfering ribonucleic acids (siRNAs) were transfected to the cells to reverse the effects of the mini-dystrophin. Finally, a phospho-mitogen-activated protein kinase (MAPK) array assay was performed to investigate signalling pathway changes caused by dystrophin expression., Results: Cell proliferation was not affected in cells transduced with ΔR3R13, but was significantly increased in cells transduced with ΔH2R23. The fusion index of myotubes derived from both ΔR3R13- and ΔH2R23 -expressing cells was significantly compromised in comparison to myotubes derived from non-transduced cells. Dystrophin siRNA transfection restored the differentiation of ΔH2R23-expressing cells. The Erk1/2- signalling pathway is altered in cells transduced with mini-dystrophin constructs., Conclusions: Ectopic expression of dystrophin in cultured human skeletal muscle-derived cells may affect their proliferation and differentiation capacity. Caution should be taken when considering genetic correction of autologous stem cells to express dystrophin driven by a constitutive promoter.
- Published
- 2020
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