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Simple Summary: The development of skeletal anomalies in the early life stage of gilthead seabream (Sparus aurata) poses a significant challenge for farmers, affecting their profit margins, animal welfare, and the consumers' perception of the aquaculture industry. Although many factors have been considered to be causative in the development of skeletal anomalies in marine finfish, the stocking density and available swimming space represent two key parameters which can be easily manipulated by the farmers during the critical phase of pre-ongrowing (prior to being placed in sea cages). This work aims at distinguishing which among tank volume and stocking density is the driving factor eliciting the development of skeletal anomalies during the pre-ongrowing phase in gilthead seabream, a productive cycle in which many skeletal anomalies can arise, particularly those affecting the vertebral axis. The results from this work indicated that particular cranial and axis deformities affected fish in higher incidences when they were reared at higher densities. The results are discussed through an eco-evo-devo approach in relation to the potential mechanisms at play affecting the increased prevalence of skeletal anomalies found. This research represents an intriguing contribution to aquaculture with results that can be applied directly to the production methods used by fish farmers to ameliorate the skeletal and morphological quality of farmed gilthead seabream. Gilthead seabream (Sparus aurata) production is a highly valued aquaculture industry in Europe. The presence of skeletal deformities in farmed gilthead seabream represents a major bottleneck for the industry leading to economic losses, negative impacts on the consumers' perception of aquaculture, and animal welfare issues for the fish. Although past work has primarily focused on the hatchery phase to reduce the incidence of skeletal anomalies, this work targets the successive pre-ongrowing phase in which more severe anomalies affecting the external shape often arise. This work aimed to test the effects of: (i) larger and smaller tank volumes, stocked at the same density; and (ii) higher and lower stocking densities maintained in the same water volume, on the skeleton of gilthead seabream fingerlings reared for ~63 days at a pilot scale. Experimental rearing was conducted with gilthead seabream juveniles (~6.7 ± 2.5 g), which were selected as 'non-deformed' based on external inspection, stocked at three different densities (Low Density (LD): 5 kg/m3; Medium Density (MD): 10 kg/m3; High Density (HD): 20 kg/m3) in both 500 L and 1000 L tanks. Gilthead seabream were sampled for growth performance and radiographed to assess the skeletal elements at the beginning and end of the experimental trial. Results revealed that (i) LD fish were significantly longer than HD fish, although there were no differences in final weights, regardless of the water volume; (ii) an increase in the prevalence of seabream exhibiting cranial and vertebral axis anomalies was found to be associated with increased density. These results suggest that farmers can significantly reduce the presence of some cranial and axis anomalies affecting pre-ongrown gilthead seabream by reducing the stocking density. [ABSTRACT FROM AUTHOR]

The innate immune responses of mammals to microbial infections include strategies based on manipulating the local concentration of metals such as iron (Fe) and zinc (Zn), commonly described as nutritional immunity. To evaluate whether these strategies are also present in zebrafish embryos, we have conducted a series of heart cavity-localized infection experiments with Pseudomonas aeruginosa strains characterized by a different ability to acquire Zn. We have found that, 48 h after infection, the bacterial strains lacking critical components of the Zn importers ZnuABC and ZrmABCD have a reduced colonization capacity compared to the wild-type strain. This observation, together with the finding of a high level of expression of Zur-regulated genes, suggests the existence of antimicrobial mechanisms based on Zn sequestration. However, we have observed that strains lacking such Zn importers have a selective advantage over the wild-type strain in the early stages of infection. Analysis of the expression of the gene that encodes for a Zn efflux pump has revealed that at short times after infection, P. aeruginosa is exposed to high concentrations of Zn. At the same time, zebrafish respond to the infection by activating the expression of the Zn transporters Slc30a1 and Slc30a4, whose mammalian homologs mediate a redistribution of Zn in phagocytes aimed at intoxicating bacteria with a metal excess. These observations indicate that teleosts share similar nutritional immunity mechanisms with higher vertebrates, and confirm the usefulness of the zebrafish model for studying host–pathogen interactions. [ABSTRACT FROM AUTHOR]

Geostationary observations offer the unique opportunity to resolve the diurnal cycle of the Earth's Radiation Budget at the top of the atmosphere (TOA), crucial for climate-change studies. However, a drawback of the continuous temporal coverage of the geostationary orbit is the fixed viewing geometry. As a consequence, imperfections in the angular distribution models (ADMs) used in the radiance-to-flux conversion process or residual angular-dependent narrowband-to-broadband conversion errors can result in systematic errors of the estimated radiative fluxes. In this work, focusing on clear-sky reflected TOA observations, we compare the overlapping views from Meteosat Second Generation satellites at 0° and 41.5 °E longitude which enable a quantification of viewing-angle-dependent differences. Using data derived from the Spinning Enhanced Visible and InfraRed Imager (SEVIRI), we identify some of the main sources of discrepancies, and show that they can be significantly reduced at the level of one month. This is achieved, separately for each satellite, via a masking procedure followed by an empirical fit at the pixel-level that takes into account all the clear-sky data from that satellite, calculated separately per timeslot of the day, over the month of November 2016. The method is then applied to each month of 2017, and gives a quadratic mean of the albedo root-mean squared difference over the dual-view region which is comparable from month to month, with a 2017 average value of 0.01. Sources of discrepancies include the difficulty to estimate the flux over the sunglint ocean region close to the limbs, the fact that the data processing does not include dedicated angular distribution models for the aerosol-over-ocean case, and the existence of an observer-dependent diurnal-asymmetry artefact affecting the clear-sky-albedo dependence on the solar zenith angle particularly over land areas. [ABSTRACT FROM AUTHOR]