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Major depressive disorder is a widespread condition with antidepressants as the main pharmacological treatment. However, some patients experience concerning adverse reactions or have an inadequate response to treatment. Analytical chromatographic techniques, among other techniques, are valuable tools for investigating medication complications, including those associated with antidepressants. Nevertheless, there is a growing need to address the limitations associated with these techniques. In recent years, electrochemical (bio)sensors have garnered significant attention due to their lower cost, portability, and precision. Electrochemical (bio)sensors can be used for various applications related to depression, such as monitoring the levels of antidepressants in biological and in environmental samples. They can provide accurate and rapid results, which could facilitate personalized treatment and improve patient outcomes. This state-of-the-art literature review aims to explore the latest advancements in the electrochemical detection of antidepressants. The review focuses on two types of electrochemical sensors: Chemically modified sensors and enzyme-based biosensors. The referred papers are carefully categorized according to their respective sensor type. The review examines the differences between the two sensing methods, highlights their unique features and limitations, and provides an in-depth analysis of each sensor.

The aims of this study were to characterize the exposure of pregnant women living in Portugal to 3-phenoxybenzoic acid (3-PBA) and to evaluate the association of this exposure with maternal outcomes and newborn anthropometric measures. We also aimed to compare exposure in summer with exposure in winter. Pregnant women attending ultrasound scans from April 2018 to April 2019 at a central hospital in Porto, Portugal, were invited to participate. Inclusion criteria were: gestational week between 10 and 13, confirmed fetal vitality, and a signature of informed consent. 3-PBA was measured in spot urine samples by gas chromatography with mass spectrometry (GC-MS). The median 3-PBA concentration was 0.263 (0.167; 0.458) μg/g creatinine (n = 145). 3-PBA excretion was negatively associated with maternal pre-pregnancy body mass index (BMI) (p = 0.049), and it was higher during the summer when compared to winter (p < 0.001). The frequency of fish or yogurt consumption was associated positively with 3-PBA excretion, particularly during the winter (p = 0.002 and p = 0.015, respectively), when environmental exposure is low. Moreover, 3-PBA was associated with levothyroxine use (p = 0.01), a proxy for hypothyroidism, which could be due to a putative 3-PBA—thyroid hormone antagonistic effect. 3-PBA levels were not associated with the anthropometric measures of the newborn. In conclusion, pregnant women living in Portugal are exposed to 3-PBA, particularly during summer, and this exposure may be associated with maternal clinical features., This article was supported by national funds through the FCT Foundation for Science and Technology, I.P., within the scope of the projects RISE - LA/P/0053/2020; CINTESIS, R&D UNIT (reference UIDB/4255/2020) and LAQV (references: UIDB/50006/2020 AND UIDP/50006/2020). Virgínia Cruz Fernandes was funded by FCT/MCTES (Foundation for Science and Technology and Ministry of Science, Technology and Higher Education) and the ESF (European Social Fund) through NORTE 2020 (North Region Operational Program) through a grant of Post-Doc (reference SFRH/BPD/109153/2015). Juliana Guimarães was funded by FCT/MCTES (Foundation for Science and Technology and Ministry of Science, Technology and Higher Education) under CINTESIS by a PhD scholarship (reference UI/BD/152087/2021). The authors would like to thank the kind participation of all pregnant women and the support of the recruitment activities by the health professionals of the Department of Obstetrics, Centro Hospitalar Universitário S. João, Porto, Portugal and also to the professionals of REQUIMTE/LAQV, Instituto Superior de Engenharia, Politécnico do Porto, for their collaboration in carrying out the analyzes of the 3-PBA metabolite. This work received support from PT national funds (FCT/MCTES, Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) through the projects LA/P/0053/2020; UIDB/4255/2020; UIDB/5006/2020 and UIDP/50006/2020. Virgínia Cruz Fernandes thanks FCT/MCTES (Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) and ESF (European Social Fund) through NORTE 2020 (Programa Operacional Região Norte) for his/her Post-Doc grant ref. SFRH/BPD/109153/2015). Juliana Guimarães was funded by FCT/MCTES (Foundation for Science and Technology and Ministry of Science, Technology and Higher Education) under CINTESIS by a PhD scholarship (reference UI/BD/152087/2021).

Chestnut (Castanea sativa) shells (CSs), an undervalued agro-industrial biowaste, have arisen as a source of bioactive compounds with promising health-promoting effects. This study attempted, for the first time, to develop a functional food, namely cookies, using a CS extract obtained by an eco-friendly technology (subcritical water extraction). The cookies were characterized regarding their nutritional composition, total phenolic and flavonoid contents (TPC and TFC, respectively), antioxidant/antiradical activities, phenolic profile, and sensory evaluation. The results demonstrated that the CS-extract-enriched cookies were mainly composed of carbohydrates (53.92% on dry weight (dw)), fat (32.62% dw), and fiber (5.15% dw). The phenolic profile outlined by HPLC-PDA revealed the presence of phenolic acids, flavonoids, and hydrolysable tannins, attesting to the high TPC and TFC. The in vitro antioxidant/antiradical effects proved the bioactivity of the functional cookies, while the sensory evaluation unveiled excellent scores on all attributes (≥6.25). The heatmap diagram corroborated strong correlations between the TPC and antioxidant/antiradical properties, predicting that the appreciated sensory attributes were closely correlated with high carbohydrates and phenolic compounds. This study encourages the sustainable recovery of antioxidants from CSs and their further employment as an active nutraceutical ingredient in functional cookies., This work received financial support from national funds (UIDB/50006/2020), project PTDC/ASP-AGR/29277/2017-Castanea sativa shells as a new source of active ingredients for Functional Food and Cosmetic applications: a sustainable approach, and project 5537 DRI, Sérvia 2020/21 from the Portuguese-Serbia Bilateral Cooperation-Development of functional foods, incorporating a chestnut shell extract obtained by subcritical water, supported by national funds by FCT/MCTES and co-supported by Fundo Europeu de Desenvolvimento Regional (FEDER) throughout COMPETE 2020-Programa Operacional Competitividade e Internacionalização (POCI-01-0145-FEDER-029277). Authors also thanks the project SYSTEMIC “an integrated approach to the challenge of sustainable food systems: adaptive and mitigatory strategies to address climate change and malnutrition“. The Knowledge hub on Nutrition and Food Security, has received funding from national research funding parties in Belgium (FWO), France (INRA), Germany (BLE), Italy (MIPAAF), Latvia (IZM), Norway (RCN), Portugal (FCT), and Spain (AEI) in a joint action of JPI HDHL, JPI-OCEANS and FACCE-JPI launched in 2019 under the ERA-NET ERA- HDHL (n◦ 696295). Jaroslava Švarc-Gajić and Tanja Brezo-Borjan are grateful to the Science Fund of the Republic of Serbia (Grant No. 7747845, In situ pollutants removal from waters by sustainable green nanotechnologies—CleanNanoCatalyze), and to the Ministry of education, science and technological development of the Republic of Serbia (Grant No 451-03-68/2020–14/200134).The authors kindly thank Sortegel (Sortes, Portugal) for the samples. Diana Pinto (SFRH/BD/144534/2019) is thankful for her Ph.D. grant financed by FCT/MCTES and POPH-QREN and supported by funds from European Union (EU) and Fundo Social Europeu (FSE) through Programa Operacional Regional Norte. Francisca Rodrigues (CEECIND/01886/2020), Elsa F. Vieira (CEECIND/03988/2018), and Manuela M. Moreira (CEECIND/02702/2017) are thankful for their contracts financed by FCT/MCTES—CEEC Individual 2020 Program Contract. Anna Vallverdú-Queralt thanks the Spanish Ministerio de Ciencia, Innovación y Universidades for the Ramon y Cajal contract (RYC-2016-19355). The authors kindly thank Sortegel (Sortes, Portugal) for the samples. Diana Pinto (SFRH/BD/144534/2019) is thankful for her Ph.D. grant financed by FCT/MCTES and POPHQREN and supported by funds from European Union (EU) and Fundo Social Europeu (FSE) through Programa Operacional Regional Norte. Francisca Rodrigues (CEECIND/01886/2020), Elsa F. Vieira (CEECIND/03988/2018), and Manuela M. Moreira (CEECIND/02702/2017) are thankful for their contracts financed by FCT/MCTES—CEEC Individual 2020 Program Contract. Anna VallverdúQueralt thanks the Spanish Ministerio de Ciencia, Innovación y Universidades for the Ramon y Cajal contract (RYC-2016-19355).

In the last years, the demand for new eco-friendly ingredients has increased on the cosmetic market. Consumers are more aware of sustainable principles and, simultaneously, more conscious regarding the skin aging process. Chestnut (Castanea sativa) shells are a food by-product produced in high quantities in Europe. This waste has been described as a rich source of phenolic compounds with skin biological effects, such as antioxidant, antiradical, and anti-inflammatory activities. Despite the huge number of assays reporting the richness of chestnut shell extracts in bioactive compounds as well as the development of cosmetic formulations containing these extracts, no in vivo assays have assessed their clinical efficacy in human volunteers. The aim of this study was to evaluate the effect on skin face parameters of a formulation containing a chestnut shell extract in human volunteers (n = 22) who applied the product twice per day, for 56 days. For that, biophysical techniques, including Corneometer®, Cutometer®, and PrimosPremium, were employed, allowing the quantification of skin hydration and firmness, as well as of wrinkles’ depth and volume and wrinkles’ skin roughness. The results demonstrated that the formulation led to a slight decrease in roughness and wrinkles’ depth, although no significant differences with respect to a placebo were observed. In addition, a clear improvement of skin hydration was achieved (t0 = 54.00 Arbitrary Units (A.U.) and t56 = 58.62 A.U.). In contrast to the placebo, the active formulation increased skin firmness up to 31.76% in 50.00% of the volunteers, but without significant differences with respect to the placebo, probably due to the short period of treatment. A long-term use of the product is recommended to possibly observe significant differences in all parameters., This research was funded by the project PTDC/ASP-AGR/29277/2017—Castanea sativa shells as a new source of active ingredients for Functional Food and Cosmetic applications: a sustainable approach, supported by national funds by FCT/MCTES and co-supported by the Fundo Europeu de Desenvolvimento Regional (FEDER) throughout COMPETE 2020—Programa Operacional Competitividade e Internacionalização (POCI-01-0145-FEDER-029277). It was also funded by the projects UIDB/50006/2020 and UIDP/50006/2020 through national funds. This work was also financed by national funds from FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences—UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. The authors are thankful to Sortegel for the chestnut samples and their availability during this work. The authors are thankful to Evonik for the samples. Ana Margarida Silva is thankful for the Ph.D. grant (SFRH/BD/144994/2019) financed by POPH-QREN and subsidized by the European Science Foundation and Ministério da Ciência, Tecnologia e Ensino Superior. Francisca Rodrigues (CEECIND/01886/2020) is thankful for her contract financed by FCT/MCTES—CEEC Individual Program Contract

The goal of this work was to examine whether elevated iodine intake was associated with adverse effects on IQ among school-age children in Portugal. In a representative sample of children from the north of the country, IQ percentiles by age (assessed with Raven’s Colored Progressive Matrices) were dichotomized to, This project was funded through grants by the Public Health Initiatives Programme (PT06), financed by EEA Grants Financial Mechanism 2009–2014, and supported by FEDER through the operation POCI-01-0145-FEDER-007746 funded by the Programa Operacional Competitividade e Internacionalização—COMPETE2020 and by National Funds through FCT—Fundação para a Ciência e a Tecnologia, I.P., within CINTESIS, R&D Unit (reference UIDP/4255/2020) and within the scope of the project RISE, Associated Laboratory (reference LA/P/0053/2020). DP and VCF also received individual funding from Fundação para a Ciência e a Tecnologia (SFRH/BPD/109158/2015 in the first case; SFRH/BPD/109153/2015, UIDB/50006/2020, UIDP/50006/2020, and LA/P/0008/2020 in the second case)

Microplastics (MP) are spread into all ecosystems and represent a threat to the equilibrium of the environment and human health, not only due to their intrinsic characteristics but also to their action as effective carriers of contaminants, such as pesticides, pharmaceuticals, polychlorinated biphenyls and polycyclic aromatic hydrocarbons. The pesticide α-endosulfan is persistent and spread in the environment. The MP are another possible way of dissemination to be considered in the fate of this pesticide. The adsorption dynamics of α-endosulfan by six different MP (low-density polyethylene—LDPE, polyethylene-co-vinyl acetate, unplasticized polyvinyl chloride, polyamide 6, polystyrene granule, polypropylene granule) with different sizes/shapes and chemical compositions were evaluated. The most critical situation was identified for the system LDPE (particle size < 300 μm). Equilibrium studies (48 h equilibrium time) were performed for distilled, tap and filtered river water. Based on the Langmuir model parameters, the highest maximum adsorption capacity was obtained for distilled water, followed by filtered river and tap waters (i.e., 366 ± 39, 247 ± 38, 157 ± 22 μg/g). The obtained results demonstrate the important role that microplastics may have in the fate and transport of pesticides and their potentially harmful effect on the environment, which requires further investigation, This research was funded by the Associate Laboratory for Green Chemistry-LAQV, which received financial support from UIDB/50006/2020, UIDP/50006/2020, and LA/P/0008/2020 by the Fundação para a Ciência e a Tecnologia (FCT)/Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) through national funds. This research was funded through the 2019–2020 Biodiversa & Water JPI joint call for research proposals, under the BiodivRestore ERA-Net COFUND programme, DivRestore/0002/2020, and with the funding organisation FCT, Portugal. Virgínia Cruz Fernandes thanks FCT for the financial support through a postdoctoral fellowship (SFRH/BPD/109153/2015). Also, Sílvia Daniela Martinho thanks FCT for the financial support through a doctoral fellowship (SFRH/BD/13595/2022). The authors are greatly indebted to all financing sources.

Currently, there is no known cure for neurodegenerative disease. However, the available therapies aim to manage some of the symptoms of the disease. Human neurodegenerative diseases are a heterogeneous group of illnesses characterized by progressive loss of neuronal cells and nervous system dysfunction related to several mechanisms such as protein aggregation, neuroinflammation, oxidative stress, and neurotransmission dysfunction. Neuroprotective compounds are essential in the prevention and management of neurodegenerative diseases. This review will focus on the neurodegeneration mechanisms and the compounds (proteins, polyunsaturated fatty acids (PUFAs), polysaccharides, carotenoids, phycobiliproteins, phenolic compounds, among others) present in seaweeds that have shown in vivo and in vitro neuroprotective activity. Additionally, it will cover the recent findings on the neuroprotective effects of bioactive compounds from macroalgae, with a focus on their biological potential and possible mechanism of action, including microbiota modulation. Furthermore, gastrointestinal digestion, absorption, and bioavailability will be discussed. Moreover, the clinical trials using seaweed-based drugs or extracts to treat neurodegenerative disorders will be presented, showing the real potential and limitations that a specific metabolite or extract may have as a new therapeutic agent considering the recent approval of a seaweed-based drug to treat Alzheimer’s disease, This work was financed by FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operacional Programme for Competitiveness and Internationalisation (POCI), and by Portuguese funds through FCT—Fundação para a Ciência e a Tecnologia in the framework of the project POCI-01-0145-FEDER-030240—PTDC/OCE-ETA/30240/2017—SilverBrain-From sea to brain: Green neuroprotective extracts for nanoencapsulation and functional food production. This work was supported by projects REQUIMTE/LAQV—UIDB/50006/2020, UIDP/50006/2020, and LA/P/0008/2020 financed by FCT/Ministério da Ciência, Tecnologia e Ensino Superior (MCTES), through national funds

Plastics have been one of the most useful materials in the world, due to their distinguishing characteristics: light weight, strength, flexibility, and good durability. In recent years, the growing consumption of plastics in industries and domestic applications has revealed a serious problem in plastic waste treatments. Pollution by microplastics has been recognized as a serious threat since it may contaminate all ecosystems, including oceans, terrestrial compartments, and the atmosphere. This micropollutant is spread in all types of environments and is serving as a “minor but efficient” vector for carrier contaminants such as pesticides, pharmaceuticals, metals, polychlorinated biphenyls (PCBs), and polycyclic aromatic hydrocarbons (PAHs). The need to deeply study and update the evolution of microplastic sources, toxicology, extraction and analysis, and behavior is imperative. This review presents an actual state of microplastics, addressing their presence in the environment, the toxicological effects and the need to understand their extent, their interactions with toxic pollutants, the problems that arise in the definition of analytical methods, and the possible alternatives of treatments, This research was funded by the Associate Laboratory for Green Chemistry-LAQV, which received financial support from UIDB/50006/2020, UIDP/50006/2020, and LA/P/0008/2020 by the Fundação para a Ciência e a Tecnologia (FCT)/Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) through national funds. The research was funded also by FCT and BiodivRestore Joint Call 2020–2021—European Union’s Horizon 2020 research and innovation programme under grant agreement No 101003777-BiodivRestore-406/DivRestore/0002/2020-BioReset-“Biodiversity restoration and conservation of inland water ecosystems for environmental and human well-being”. Virgínia Cruz Fernandes thanks FCT for the financial support through a postdoctoral fellowship (SFRH/BPD/109153/2015). The authors are greatly indebted to all financing sources

Neuroprotection is a need that remains unmet in treating chronic neurodegenerative disorders, despite decades of extensive research. To find new neuroprotective compounds, extracts of Himanthalia elongata (L.) S.F.Gray and of Eisenia bicyclis (Kjellman) Setchell were obtained through subcritical water extraction applying a four-step temperature gradient. The fractions obtained were screened against brain enzymes involved in neurodegenerative etiology, namely in Alzheimer’s and Parkinson’s diseases, and against reactive oxygen and nitrogen species, all contributing factors to the progression of neurodegeneration. Results showed no significant enzyme inhibition but strong radical scavenging activities, particularly in the fourth fraction, extracted at the highest temperature (250 °C), highlighting their ability to retard oxidative and nitrosative stresses. At higher temperatures, fractions were composed of phenolic compounds and Maillard reaction products, a combination that contributed to their antioxidant activity and, consequently, their neuroprotective properties. All fractions were evaluated for the presence of iodine, 14 organochlorine and 7 organophosphorus pesticides, and pharmaceuticals used in Alzheimer’s and Parkinson’s diseases (14), psychiatric drugs (8), and metabolites (8). The fractions studied did not present any of the screened contaminants, and only fraction 1 of E. bicyclis should be used with caution due to iodine content., This work was supported by projects REQUIMTE/LAQV—UIDB/50006/2020 and UIDP/50006/2020, financed by FCT/Ministério da Ciência, Tecnologia e Ensino Superior (MCTES), through national funds. A.P. also acknowledges FCT for the project IF/01146/2015, and V.C.F. thanks FCT for the financial support through a postdoctoral fellowship (SFRH/BPD/109153/2015).

An amperometric immunosensor was developed for the analysis of the major egg-white allergen ovotransferrin (Gal d 3) in commercial food products because the (accidental) intake, skin contact with, and/or inhalation of eggs can lead to severe disorders in allergic individuals. Employing a sandwich-type immunosensing strategy, screen-printed carbon electrodes (SPCE) were biomodified with anti-Gal d 3 (capture) antibodies, and the allergen’s detection was achieved with anti-Gal d 3 antibodies labelled with horseradish peroxidase (HRP). The 3,3′,5,5′-tetramethylbenzidine (TMB)/H2O2 reaction with HRP was used to obtain the electrochemical (amperometric) signal. An attractive assay time of 30 min and a remarkable analytical performance was achieved. The quantification range was established between 55 and 1000 ng·mL−1, with a limit of detection of 16 ng·mL−1. The developed method demonstrated good precision (Vx0 = 5.5%) and provided precise results (CV < 6%). The sensor also detected extremely low amounts (down to 0.010%) of egg. The analysis of seven raw and/or cooked egg and egg-white samples indicated that food processing influences the amount of allergen. Furthermore, to assure the compliance of product labelling with EU legislation, 25 commercial food ingredients/products were analysed. The accuracy of the results was confirmed through an ELISA assay. The stability of the ready-to-use sensing surface for 20 days allows a reduction of the reagents’ volumes and cost., This research received financial support from the European Union (FEDER funds through COMPETE POCI-01-0145-FEDER-030735) and National Funds (Fundação para a Ciência e a Tecnologia (FCT)) through the project PTDC/QUI-QAN/30735/2017—TracAllerSens—Electrochemical sensors for the detection and quantification of trace amounts of allergens in food products.

Groundnuts, like peanuts, are commonly integrated into the Mediterranean dietary pattern, and their consumption has been recommended worldwide. However, reported cases of peanut allergy have increased and, therefore, commercial food tracking is essential, since in extreme cases peanut intake causes anaphylaxis. Efficient detection of peanut traces in food samples can be achieved using electrochemical immunosensors that benefit from their advantageous features such as high selectivity and sensitivity, low cost, and rapid detection. Because disposable screen-printed electrodes can be connected to portable devices, they can be used for in situ allergen analysis. In the present work, a voltammetric immunosensor was developed to quantify a major peanut allergen, Ara h 1, using screen-printed carbon electrodes (SPCE) as transducers. Distinct carbon-based nanomaterials were tested, and nanodiamonds were selected for the nanostructuration of the transducer’s surface. A sandwich-type immunoassay was performed on the nanodiamond-coated SPCEs using an alkaline phosphatase-labelled detection antibody and a mixture containing an enzymatic substrate (3-indoxyl phosphate) and silver nitrate. The immunological interaction was detected through the (linear sweep) voltammetric stripping of the enzymatically deposited silver. A linear concentration range was established between 25 and 500 ng·mL−1 (ip = (0.027 ± 0.001) [Ara h 1] + (1.41 ± 0.31), r = 0.994, n = 5), with a sensitivity of 0.342 µA·mL·ng-1·cm-2. The limits of detection (LOD) and quantification (LOQ) were 0.78 and 2.6 ng·mL−1, respectively. Other allergens, non-target proteins and food product ingredients were tested to assess the selectivity of the immunosensor. Its applicability was evaluated by analysing a set of breakfast cereals, cookies, and energetic and cereal bars.

This work characterizes the dimension and the exceptionality of 2017 large- and mega-fires that occurred in the center region of Portugal through the assessment of their impact on the ambient levels of particulate matter (PM10 and PM2.5), retrieved from local monitoring stations, and the associated public health risks. PM10 and PM2.5 concentrations were increased during the occurrence of large fires and megafires, with daily concentrations exceeding the European/national guidelines in 7&ndash, 14 and 1&ndash, 12 days of 2017 (up to 704 &micro, g/m3 for PM10 and 46 &micro, g/m3 for PM2.5), respectively. PM10 concentrations were correlated with total burned area (0.500 &lt, r &lt, 0.949, p &gt, 0.05) and with monthly total burned area/distance2 (0.500 &lt, 0.667, 0.05). The forest fires of 2017 took the life of 112 citizens. A total of 474 cases of hospital admissions due to cardiovascular diseases and 3524 cases of asthma incidence symptoms per 100,000 individuals at risk were assessed due to exposure to 2017 forest fires. Real-time and in situ PM methodologies should be combined with protection action plans to reduce public health risks. Portuguese rural stations should monitor other health-relevant pollutants (e.g., carbon monoxide and volatile organic compounds) released from wildfires to allow performing more robust and comprehensive measurements that will allow a better assessment of the potential health risks for the exposed populations.

The oil industry generates great quantities of oilseed cakes that remain after oil extraction. New technologies are required for their valorization, owing to their high nutritional value. Pumpkin, flax and hemp seed cakes were extracted by subcritical water under different conditions that included different gas atmospheres and homogenous catalysis, and for the first time their properties were directly compared. Extracts obtained in a nitrogen atmosphere, nitrogen atmosphere with the addition of a catalyst, and carbon dioxide atmosphere were chemically and nutritionally characterised. In the aqueous extracts obtained under different extraction conditions, the content of lipids, proteins and selected minerals (calcium, potassium, sodium and phosphorus) were determined. A detailed amino acid profile was determined by chromatographic analysis. The highest relative content of essential amino acids was observed in pumpkin seed extracts (51.49 ± 0.47 to 58.58 ± 0.45 mg/100 g dry extract), whereas hemp seed extracts were the richest in flavour amino acids aspartic acid, glutamic acid and alanine. Extraction in a carbon dioxide atmosphere or in nitrogen atmosphere with a HCl modifier released generally more minerals into the aqueous phase. Aqueous oilseed cake extracts demonstrated a favorable chemical composition and great nutritional value, opening new possibilities for exploitation of this biowaste. Based on the obtained results, oilseed cake extracts obtained by subcritical water have great potential to be used for the fortification of different food products, as well as in cosmetics, This research was funded by the Ministry of education, science and technological development of the Republic of Serbia, grant number 451-03-68/2020-14/200134 and from project 5537 DRI, Sérvia 2020/21–Development of functional foods incorporating a chestnut shells extract obtained by subcritical water, supported by Portuguese Foundation for Science and Technology and by FCT/MCTES through national funds. Further, this work received financial support from FEDER (through COMPETE funds), Fundação para a Ciência e Tecnologia (by project UIDB/50006/2020), and through FCT/MCTES—CEEC Individual 2018 Program Contract (CEECIND/03988/2018; Elsa F. Vieira). Funding acknowledgement is also given to the Italian Ministry of University (MIUR) call PRIN 2017, project 2017LEPH3M “PANACEA: A technology PlAtform for the sustainable recovery and advanced use of NAnostructured CEllulose from Agro-food residues.”