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To study the fatigue failure and microstructure evolution behavior of SS304, low-cycle fatigue tests are conducted at room temperature (RT), 300 °C, and 650 °C. The results indicate that, because of the influence of the dislocation walls, carbon-containing precipitates, and deformation twins, the cyclic hardening behavior is presented at RT. However, different from the cyclic hardening behavior at RT, the cyclic softening behavior of SS304 can be observed due to the dynamic recovery and recrystallization containing dislocation rearrangement and annihilation at 300 °C and 650 °C. In addition, two fatigue crack initiation modes are observed. At RT, the single fatigue crack initiation mode is observed. At high temperatures, multiple crack initiation modes are presented, resulting from the degradation of material properties. Furthermore, a new fatigue life prediction model considering the temperature is conducted as a reference for industrial applications. [ABSTRACT FROM AUTHOR]

In this study, the addition of oregano oil chitosan nanoparticles (OEO-CSNPs) was conducted to enhance the comprehensive properties of gelatin films (GA), and the optimal addition ratio of nanoparticles was determined for its application in the preservation of mullet. Oregano oil chitosan nanoparticles were organically combined with gelatin at different concentrations (0%, 2%, 4%, 6% and 8%) to obtain oregano oil–chitosan nanoparticle–GA-based composite films (G/OEO-CSNPs), and thereafter G/OEO-CSNPs were characterized and investigated for their preservative effects on mullet. Subsequent analysis revealed that OEO-CSNPs were uniformly dispersed in the GA matrix, and that G/OEO-CSNPs had significantly improved mechanical ability, UV-visible light blocking performance and thermal stability. Furthermore, the nanoparticles exhibited excellent antioxidant and antibacterial properties, and they improved the films' suitability as edible packaging. The attributes of the G/OEO-CSNPs were optimized, the films had the strongest radical scavenging and lowest water solubility, and electron microscopy also showed nanoparticle penetration into the polymer when the concentration of OEO-CSNPs was 6% (thickness = 0.092 ± 0.001, TS = 47.62 ± 0.37, E = 4.06 ± 0.17, water solubility = 48.00 ± 1.11). Furthermore, the GA-based composite film containing 6% OEO-CSNPs was able to inhibit microbial growth, slow fat decomposition and protein oxidation, reduce endogenous enzyme activity, and delay the spoilage of mullet during the refrigeration process, all of which indicate its excellent potential for meat preservation application. [ABSTRACT FROM AUTHOR]

The effect of strain rate and temperature on the thermomechanical behavior and microstructure of MarBN steel is studied with the strain rates of 5 × 10−3 and 5 × 10−5 s−1 from room temperature (RT) to 630 °C. At high strain rates of 5 × 10−3 s−1, the Holloman and Ludwigson equations can better predict tensile plastic properties. In contrast, under low strain rates of 5 × 10−5 s−1, coupling of the Voce and Ludwigson equations appears to predict the flow relationship at RT, 430, and 630 °C. However, the deformation microstructures have the same evolution behavior under strain rates and temperatures. Geometrically necessary dislocations appear along the grain boundaries and increase the dislocation density, which results in the formation of the low-angle grain boundaries and a decrease in the number of twinning. The strengthening sources of MarBN steel include grain boundary strengthening, dislocation interactions, and multiplication. The fitted R2 values of these models (JC, KHL, PB, VA, ZA) to plastic flow stress at 5 × 10−5 s−1 are greater than 5 × 10−3 s−1 for MarBN steel. Due to the flexibility and minimum fitting parameters, the phenomenological models of JC (RT and 430 °C) and KHL (630 °C) give the best prediction accuracy under both strain rates. [ABSTRACT FROM AUTHOR]

The presence of mycotoxins in cereals can pose a significant health risk to animals and humans. China is one of the countries that is facing cereal contamination by mycotoxins. Treating mycotoxin-contaminated cereals with established physical and chemical methods can lead to negative effects, such as the loss of nutrients, chemical residues, and high energy consumption. Therefore, microbial detoxification techniques are being considered for reducing and treating mycotoxins in cereals. This paper reviews the contamination of aflatoxins, zearalenone, deoxynivalenol, fumonisins, and ochratoxin A in major cereals (rice, wheat, and maize). Our discussion is based on 8700 samples from 30 provincial areas in China between 2005 and 2021. Previous research suggests that the temperature and humidity in the highly contaminated Chinese cereal-growing regions match the growth conditions of potential antagonists. Therefore, this review takes biological detoxification as the starting point and summarizes the methods of microbial detoxification, microbial active substance detoxification, and other microbial inhibition methods for treating contaminated cereals. Furthermore, their respective mechanisms are systematically analyzed, and a series of strategies for combining the above methods with the treatment of contaminated cereals in China are proposed. It is hoped that this review will provide a reference for subsequent solutions to cereal contamination problems and for the development of safer and more efficient methods of biological detoxification. [ABSTRACT FROM AUTHOR]

The uniaxial tensile behavior of MarBN steel with a constant strain rate of 5 × 10−5 s−1 under various temperatures ranging from room temperature to 630 °C was analyzed. This study aimed to identify the effect of the temperature on the tensile behavior and to understand the microstructure deformation by electron backscatter diffraction. The tensile results showed that the yield and ultimate tensile strength decreased with increasing temperature. Serrated flow was observed from 430 °C to 630 °C. The electron backscatter diffraction analysis showed that the low-angle grain boundaries decreased at the medium deformation and increased at the maximum deformation. In contrast, they decreased with increasing temperatures. In addition, the number of voids increased with the increasing plastic strain. As the strain increased, the voids joined together, and the tiny cracks became larger and failed. Three mechanisms were responsible for the tensile deformation failure at various temperatures: grain rotation, the formation and rearrangement of low angle grain boundaries, and void nucleation and propagation. Finally, the formation of the low-angle grain boundaries and voids under different degrees of deformation is discussed. [ABSTRACT FROM AUTHOR]

To reduce harmful gas emission and improve the operational efficiency, advanced ultra-supercritical power plants put forward higher requirements on the high temperature mechanical properties of applied materials. In this paper, the tensile behavior and deformation mechanisms of MarBN steel are discussed at different strain rates (5 × 10−3 s−1, 5 × 10−4 s−1, and 5 × 10−5 s−1) under room temperature and 630 °C. The results show that the tensile behavior of the alloy is dependent on temperature and strain rate, which derived from the balance between the average dislocation velocity and dislocation density. Furthermore, observed dynamic recrystallized grains under severe deformation reveal the existence of dynamic recovery at 630 °C, which increases the elongation compared to room temperature. Finally, three typical constitutive equations are used to quantitatively describe the tensile deformation behavior of MarBN steel under different strain rates and temperatures. Meanwhile, the constitutive model of flow stress for MarBN steel is developed based on the hyperbolic sine law. [ABSTRACT FROM AUTHOR]

Derivates of natural products have been wildly utilized in the treatment of malignant tumors. Isorhamnetin (ISO), a most important active ingredient derived from flavonoids, shows great potential in tumor therapy. However, the therapeutic effects of ISO on gastric cancer (GC) remain unclear. Here, we demonstrate that ISO treatment dramatically inhibited the proliferation of two types of GC cells (AGS-1 and HGC-27) both in vitro and in vivo in time- and dose-dependent manners. These results are consistent with the transcriptomic analysis of ISO-treated GC cells, which yielded hundreds of differentially expressed genes that were enriched with cell growth and apoptosis. Mechanically, ISO treatment initiated the activation of caspase-3 cascade and elevated the expression of mitochondria-associated Bax/Bcl-2, cytosolic cytochrome c, followed by the activation of the cleavage of caspase-3 as well as poly ADP-ribose polymerase (PARP), resulting in the severe reduction of the mitochondrial potential and the accumulation of reactive oxygen species (ROS), while pre-treatment of the caspase-3 inhibitor could block the anti-tumor effect. Therefore, these results indicate that ISO treatment induces the apoptosis of GC cells through the mitochondria-dependent apoptotic pathway, providing a potential strategy for clinical GC therapy. [ABSTRACT FROM AUTHOR]

Tensile deformation behavior and microstructure of nickel-base superalloy Inconel 625 are investigated under different strain rates of 5 × 10−4 s−1 and 5 × 10−5 s−1. According to the experimental results, yield strength and ultimate tensile strength of the alloy increase with the increase in strain rate in room temperature. Microstructure results indicate that the size of dimples is smaller in the tensile fracture surface at low strain rate than the high strain rate, and the number of dimples is also related to the strain rates and twins appear earlier in the specimens with higher strain rates. Apart from Hollomon and Ludwik functions, a new formula considering the variation trend of strength in different deformation stages is deduced and introduced, which fit closer to the tensile curves of the 625 alloy used in the present work at both strain rates. Furthermore, the Schmid factors of tensile samples under two strain rates are calculated and discussed. In the end, typical work hardening behavior resulting from the dislocations slip behavior under different strain rates is observed, and a shearing phenomenon of slip lines cross through the δ precipitates due to the movement of dislocations is also be note. [ABSTRACT FROM AUTHOR]

Exosomes are essential early biomarkers for health monitoring and cancer diagnosis. A prerequisite for further investigation of exosomes is the isolation, which is technically challenging due to the complexity of body fluids. This paper presents the development of an integrated microfluidic chip for exosomes isolation, which combines the traditional immunomagnetic bead-based protocol and the recently emerging microfluidic approach, resulting in benefits from both the high-purity of the former and the automated continuous superiority of the latter. The chip was designed based on an S-shaped micromixer with embedded baffle. The excellent mixing efficiency of this micromixer compared with Y-shaped and S-shaped micromixers was verified by simulation and experiments. The photolithography technique was employed to fabricate the integrated microfluidic chip, and the manufacturing process was elucidated. We finally established an experimental platform for exosomes isolation with the fabricated microfluidic chip built in. Exosomes isolation experiments were conducted using this platform. The distribution and morphology of the isolated exosomes were observed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Quantitative size analyses based on transmission electron micrographs indicated that most of the obtained particles were between 30 and 150 nm. Western blot analyses of the isolated exosomes and the serum were conducted to verify the platform's capability of isolating a certain subpopulation of exosomes corresponding to specified protein markers (CD63). The complete time for isolation of 150 μL serum samples was approximately 50 min, which was highly competitive with the reported existing protocols. Experimental results proved the capacity of the established integrated microfluidic chip for exosomes isolation with high purity, high integrity, and excellent efficiency. The platform can be further developed to make it possible for practical use in clinical applications as a universal exosomes isolation and characterization tool. [ABSTRACT FROM AUTHOR]