1. Strawberry Vein Banding Virus P6 Protein Is a Translation Trans-Activator and Its Activity Can be Suppressed by FveIF3g.
- Author
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Li, Shuai, Hu, Yahui, Jiang, Lei, Rui, Penghuan, Zhao, Qingqing, Feng, Jiying, Zuo, Dengpan, Zhou, Xueping, and Jiang, Tong
- Subjects
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CAULIMOVIRUSES , *STRAWBERRY diseases & pests , *RNA interference , *MESSENGER RNA , *NICOTIANA benthamiana - Abstract
The strawberry vein banding virus (SVBV) open reading frame (ORF) VI encodes a P6 protein known as the RNA silencing suppressor. This protein is known to form inclusion like granules of various sizes and accumulate in both the nuclei and the cytoplasm of SVBV-infected plant cells. In this study, we have determined that the P6 protein is the only trans-activator (TAV) encoded by SVBV, and can efficiently trans-activate the translation of downstream gfp mRNA in a bicistron derived from the SVBV. Furthermore, the P6 protein can trans-activate the expression of different bicistrons expressed by different caulimovirus promoters. The P6 protein encoded by SVBV from an infectious clone can also trans-activate the expression of bicistron. Through protein-protein interaction assays, we determined that the P6 protein could interact with the cell translation initiation factor FveIF3g of Fragaria vesca and co-localize with it in the nuclei of Nicotiana benthamiana cells. This interaction reduced the formation of P6 granules in cells and its trans-activation activity on translation. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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