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Mixed data is a combination of continuous and categorical variables and occurs frequently in fields such as agriculture, remote sensing, biology, medical science, marketing, etc., but only limited work has been done with this type of data. In this study, data on continuous and categorical characters of 452 genotypes of cotton (Gossypium hirsutum) were obtained from an experiment conducted by the Central Institute of Cotton Research (CICR), Sirsa, Haryana (India) during the Kharif season of the year 2018–2019. The machine learning (ML) classifiers/models, namely k-nearest neighbor (KNN), Classification and Regression Tree (CART), C4.5, Naïve Bayes, random forest (RF), bagging, and boosting were considered for cotton genotypes classification. The performance of these ML classifiers was compared to each other along with the linear discriminant analysis (LDA) and logistic regression. The holdout method was used for cross-validation with an 80:20 ratio of training and testing data. The results of the appraisal based on hold-out cross-validation showed that the RF and AdaBoost performed very well, having only two misclassifications with the same accuracy of 97.26% and the error rate of 2.74%. The LDA classifier performed the worst in terms of accuracy, with nine misclassifications. The other performance measures, namely sensitivity, specificity, precision, F1 score, and G-mean, were all together used to find out the best ML classifier among all those considered. Moreover, the RF and AdaBoost algorithms had the highest value of all the performance measures, with 96.97% sensitivity and 97.50% specificity. Thus, these models were found to be the best in classifying the low- and high-yielding cotton genotypes. [ABSTRACT FROM AUTHOR]