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Legumes enhance pasture health and soil productivity by fixing atmospheric nitrogen and boosting soil microbiota. We investigated the effects of tropical pasture legumes, including butterfly pea (Clitoria ternatea), seca stylo (Stylosanthes scabra), desmanthus (Desmanthus virgatus), lablab (Lablab purpureus), and Wynn cassia (Chamaecrista rotundifolia), on the soil microbial community and buffel grass (Cenchrus ciliaris) gene expression. Additionally, we explored the impact of a phytogenic bioactive product (PHY) in the coculture system. A pot trial using soil enriched with cow paunch compost included four treatments: monoculture of buffel grass and five legume species with and without PHY supplementation and coculture of buffel grass with each legume species with and without PHY supplementation. Actinobacteriota and Firmicutes were the dominant bacterial phyla. Regardless of PHY application, the coculture of buffel grass with legumes positively influenced microbial composition and diversity. Transcriptomic analysis revealed significant gene expression changes in buffel grass shoots and roots, with each legume uniquely affecting nitrogen metabolism. Lablab and Wynn cassia exhibited similarities in modulating metabolic processes, butterfly pea contributed to mycotoxin detoxification, and desmanthus balanced cell death and growth. Seca stylo enhanced root cell growth and regeneration. These findings offer insights for optimizing legume–grass coculture systems, enhancing soil activity and promoting sustainable agriculture. [ABSTRACT FROM AUTHOR]

Simple Summary: The last decade of intensive research on the role and importance of intestinal microbial communities has brought abundant novel information and a high appreciation of microbiome contribution to medical and veterinary research that stretches beyond probiotic and pathogenic effects. We can now investigate the complex microbial community inhabiting major host cavities and colonising organs. In poultry research, it is common to collect cloacal swabs or faecal droppings for non-invasive sample collection or caecum and other intestinal origins to investigate their specific microbial communities. Litter samples are less frequently collected for microbial community investigation despite them being in close contact with birds and playing a significant role in microbiota colonisation, development, and maturation. Here, we explored the microbiota of litter and compared them with selected gut sections and bird health status. Probiotics provided from hatch have a major influence on microbiota development, and together with environmental and bedding microbiota, shape the microbial community of the litter. We investigated the influence of probiotic supplementation and a leaky gut challenge induced using dexamethasone (DEX) on the litter microbial community and litter parameters. The probiotic product was a mix of three Bacillus amyloliquefaciens strains. The litter microbiota were compared to the microbial communities from other gut sections. The litter samples had higher microbial diversity compared to the caecum, gizzard, jejunum, and jejunal mucosa. The high similarity between the litter phylum-level microbiota and gizzard microbiota detected in our study could be a consequence of ingested feed and litter passing through the gizzard. Moreover, the litter microbial community is fundamentally distinct from the intestinal microbiota, as evidenced by the number of genera present in the litter but absent from all the intestinal sections and vice versa. Furthermore, LEfSe analysis identified distinct microbial taxa across different groups, with specific genera associated with different treatments. In terms of litter quality, the birds in the DEX groups had a significantly higher moisture content, indicating successful leaky gut challenge, while probiotic supplementation did not significantly affect the moisture levels. These findings provide comprehensive insights into the distinct microbiota characteristics of litter. [ABSTRACT FROM AUTHOR]

This study explores the impact of a novel phytogenic product containing citric acid, carvacrol, and cinnamaldehyde on buffel grass (Cenchrus ciliaris) seedling emergence. A dilution series of the phytogenic solution revealed a concentration range that promoted seedling emergence, with an optimal concentration of 0.5%. Transcriptomic analysis using RNA-seq was performed to investigate gene expression changes in seedlings under the influence of the phytogenic product. The results revealed that the phytogenic treatment significantly altered the gene expression, with a prevalent boost in transcriptional activity compared to the control. Functional analysis indicated the positive alteration of key metabolic pathways, including the tricarboxylic acid (TCA) cycle, glycolysis, and pentose phosphate pathways. Moreover, pathways related to amino acids, nucleotide biosynthesis, heme biosynthesis, and formyltetrahydrofolate biosynthesis showed substantial modulation. The study provides valuable insights into the molecular mechanisms underlying the phytogenic product's effects on grass seedling establishment and highlights its ability to promote energy metabolism and essential biosynthetic pathways for plant growth. [ABSTRACT FROM AUTHOR]

Pasture production is vital in cattle farming as it provides animals with food and nutrients. Australia, as a significant global beef producer, has been experiencing pasture dieback, a syndrome of deteriorating grassland that results in the loss of grass and the expansion of weeds. Despite two decades of research and many remediation attempts, there has yet to be a breakthrough in understanding the causes or mechanisms involved. Suggested causes of this phenomenon include soil and plant microbial pathogens, insect infestation, extreme heat stress, radiation, and others. Plants produce a range of phytomolecules with antifungal, antibacterial, antiviral, growth-promoting, and immunostimulant effects to protect themselves from a range of environmental stresses. These products are currently used more in human and veterinary health than in agronomy. In this study, we applied a phytogenic product containing citric acid, carvacrol, and cinnamaldehyde, to investigate its ability to alleviate pasture dieback. The phytogenic liquid-based solution was sprayed twice, one week apart, at 5.4 L per hectare. The soil microbial community was investigated longitudinally to determine long-term effects, and pasture productivity and plant morphometric improvements were explored. The phytogenic liquid significantly improved post-drought recovery of alpha diversity and altered temporal and spatial change in the community. The phytogenic liquid reduced biomarker genera associated with poor and polluted soils and significantly promoted plant and soil beneficial bacteria associated with plant rhizosphere and a range of soil benefits. Phytogenic liquid application produced plant morphology improvements and a consistent enhancement of pasture productivity extending beyond 18 months post-application. Our data show that phytogenic products used in the livestock market as an alternative to antibiotics may also have a beneficial role in agriculture, especially in the light of climate change-related soil maintenance and remediation. [ABSTRACT FROM AUTHOR]

Pasture dieback is a syndrome of unknown cause affecting grasses in Australia, creating significant economic losses to farmers by reducing available livestock feed and paddock carrying capacity. RC3 is a commercial plant growth stimulant tri-sodium salt of trimercapto-S-triazine (TMT) and potassium humate as active ingredients. TMT is commonly used for soil and wastewater remediation by capturing and binding heavy metals, while potassium humate is an organic compound used as a plant growth promoter. We investigated the ability of RC3 to restore soil health and productivity under pasture dieback conditions. RC3 was applied on pasture dieback affected paddock replicate plots once, at a rate of 4 mL/m2, and soil core samples were taken weekly to analyse microbial communities. Plants were collected regularly to measure dry matter and plant morphometrics. Twenty weeks after a single application, dry matter increased in RC3 plots by 900 kg/ha compared to control plots, and at week 48, eleven months after the single application, RC3 plots showed a trend of more grass and dicot species than the control. Morphometric measures suggest minor improvements in dicotyledon plants. Alpha diversity did not change with the application of RC3. Temporal correlation analysis shows that RC3 steadily reduced the presence of genera predominant in poor soils and with extreme environmental conditions over time and prevented the decline of beneficial genera, such as Marmoricola, Actinomadura, Dactylosporangium, and mle1-7. [ABSTRACT FROM AUTHOR]

Pasture dieback (PD) is a grassland deteriorating syndrome resulting in grass loss and weed expansion in Australian pastures, with current estimates indicating that over four million hectares are affected. PD creates financial losses to the industry by reducing animal carrying capacity and producing poor-quality feed, resulting in diminished productivity. After more than a decade since PD first appeared in Australia, the causes and effective treatments are still unknown. Suggested causes include soil microbiota dysbiosis, pathogens, insects, climate change and overuse of chemical fertilisers. Sea minerals have been suggested as capable of improving plants' yield, quality, taste, and nutritional value, but were never brought into conventional practice as an alternative to chemical fertilisers. Here, we investigated the capacity of sea minerals to improve grass health and yield of PD-affected soil. The replicate plots were treated with water or with 4 mL/m2 of commercially available sea mineral product to investigate the soil chemistry profile, plant morphometrics, pasture productivity, soil microbiota profile, and microbiota-nutrient interactions. Sea mineral application significantly increased total dry matter 20 weeks after a single application, translating to an additional 967 kg/ha; this benefit was still present at 498 kg/ha eleven months post a single application. Sea mineral application improved soil microbiota by boosting beneficial taxa while reducing genera associated with arid and toxic soils. Additionally, sea mineral application increased the number of grassroots up to eleven months post a single application. Our data suggest the benefits of sea mineral application to damaged, unproductive or exhausted soils could be further explored as a natural, affordable, and non-toxic alternative to chemical fertilisers. [ABSTRACT FROM AUTHOR]

Poultry production is among the most challenging industries for pathogen control. High animal density and abundance of faecal material demand strict biosecurity measures and continual vigilance in monitoring animal health parameters. Despite this vigilance, dealing with disease outbreaks is a part of farmers' routines. Phytogenic feed additives comprised of herbs, spices, essential oils, and oleoresins have potent antimicrobial and anti-inflammatory actions. Related studies are gaining substantial interest in human and animal health worldwide. In this study, a commercial blend phytogenic feed additive was supplemented to layers in an industrial free-range production system with 20,000 birds in both control and treatment groups. At the end of the trial, the ileum tissue was sampled for RNAseq transcriptomic analysis to study the host reaction to the supplement. Phytogenic supplement significantly inhibited four cholesterol-related pathways and reduced the Arteriosclerosis disease category towards improved cardiovascular health. The supplemented birds exhibited reduced disease susceptibility for 26 cancer categories with p-values in the range from 5.23 × 10−4 to 1.02 × 10−25. Major metabolic shifts in Lipid metabolism in combination with Carbohydrate metabolism have resulted in a decrease in the Obesity category, altering the ratio of fat and carbohydrate metabolism toward lower fat storage. [ABSTRACT FROM AUTHOR]

This study was conducted to examine the effects of a plant extract mixture, a microencapsulated product composed of eugenol and garlic tincture (PE), on intestinal health in broilers under necrotic enteritis (NE) challenge. A total of 960 d-old mixed-sex Cobb 500 chicks were randomly distributed to 48-floor pens housing 20 birds per pen. Six treatments were applied: UC, unchallenged control; CC, challenged control; PE, challenged group plus PE; AM, challenged group plus antimicrobial (AM); FAP, challenged group plus a full dose of AM with PE; HAP, challenged group plus a half dose of AM with PE in starter, grower and finisher phases. Birds in the challenged groups were inoculated with Eimeria spp. on d 9 and Clostridiumperfringens on d 14. On d 16, the CC group had increased serum fluorescein isothiocyanate dextran (FITC-d), reduced villus surface area, goblet cell number, upregulated CLDN1, JAM2 genes and reduced microbial diversity compared to the UC group (p < 0.05). Birds fed PE had reduced FITC-d, increased goblet cell number and Bifidobacterium compared to the CC group (p < 0.05). Birds fed PE had reduced CLDN5 expression in male birds, and Bacteroides spp. in female birds than CC group (p < 0.05). These findings suggest that PE supplementation mitigates the effect of NE by improving the intestinal health of birds. [ABSTRACT FROM AUTHOR]

Simple Summary: The use of phytogenic products has entered mainstream use in the livestock industry as an antibiotic alternative. These products, often based on herbs and spices with established antimicrobial properties, are generally considered as safe and natural, however, they are often administered in high doses and frequency. The direct effects of these products on the livestock animals remains under-reported. Using a transcriptomics, we show that supplementing 2% oregano in feed has direct effects on gene expression in the livers of broilers with a potential range of beneficial and negative side effects. Pathogen control is re-emerging as a significant challenge to the health of both humans and animals. The livestock industry is in the process of massively replacing in-feed antibiotics with organic production friendly plant-based products. Nutrigenomics as a science of the effects of food constituents on gene expression is shedding more light on both benefits and detrimental side-effects of feed additive prolonged consumption on the host, indicating the need to understand the feed-host interactions and their influence on the host disease profile. In this study, we investigated the effects of 2% oregano powder supplementation on the liver gene expression in healthy male broilers from the hatch to 6 weeks of age. Deep RNAseq was performed on average 113.3 million paired and quality trimmed sequences per sample and four samples for the control and treatment each. The results demonstrate the severity of oregano effect on liver gene expression with substantial modifications in steroid hormone regulation, fat and carbohydrate metabolism alterations and strong influence on the host disease and function profile. Oregano supplementation was able to interfere with the transcriptional effects of a range of registered drugs and to significantly transcriptionally inhibit a range of cancer disease categories including liver cancer, and to modify fat and carbohydrate metabolism. [ABSTRACT FROM AUTHOR]