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Nowadays, consumers are more aware of the effects of their diet on their health, and thus demand natural or minimally processed food products. Therefore, research has focused on processes that assure safe products without jeopardizing their nutritional properties. In this context, this work aimed to evaluate the effects of high-pressure processing (550 MPa/3 min/15 °C, HPP) on a fruit salad (composed of melon juice and pieces of Golden apple and Rocha pear) throughout 35 days of storage at 4 °C. For the physicochemical properties analysed (browning degree, polyphenol oxidase activity, antioxidant activity (ABTS assay), and volatile profile), a freshly made fruit salad was used, while for the microbiological tests (total aerobic mesophiles, and yeast and moulds) spoiled melon juice was added to the fruit salad to increase the microbial load and mimic a challenge test with a high initial microbial load. It was determined that processed samples were more microbiologically stable than raw samples, as HPP enabled a reduction of almost 4-log units of both total aerobic mesophiles and yeasts and moulds, as well as an almost 1.5-fold increase in titratable acidity of the unprocessed samples compared to HPP samples. Regarding browning degree, a significant increase (p < 0.05) was observed in processed versus unprocessed samples (roughly/maximum 68%), while the addition of ascorbic acid decreased the browning of the samples by 29%. For antioxidant activity, there were no significant differences between raw and processed samples during the 35 days of storage. An increase in the activity of polyphenol oxidase immediately after processing (about 150%) was confirmed, which was generally similar or higher during storage compared with the raw samples. Regarding the volatile profile of the product, it was seen that the compounds associated with melon represented the biggest relative percentage and processed samples revealed a decrease in the relative quantity of these compounds compared to unprocessed. Broadly speaking, HPP was shown to be efficient in maintaining the stability and overall quality of the product while assuring microbial safety (by inactivating purposely inoculated microorganisms), which allows for longer shelf life (7 versus 28 days for unprocessed and processed fruit salad, respectively). [ABSTRACT FROM AUTHOR]

Aegle marmelos (L.) Correa is an economically and therapeutically valuable tree. It is cultivated as a fruit plant in southeast Asian countries. In this research, we investigated the allelopathy and possible allelochemicals in the leaves of A. marmelos. Aqueous methanol extracts of A. marmelos exhibited significant inhibitory effects against the growth of Lepidium sativum, Lactuca sativa, Medicago sativa, Echinochloa crusgalli, Lolium multiflorum, and Phleum pratense. Bioassay-directed chromatographic purification of the A. marmelos extracts resulted in identifying five active compounds: umbelliferone (1), trans-ferulic acid (2), (E)-4-hydroxycinnamic acid methyl ester (3), trans-cinnamic acid (4), and methyl (E)-3'-hydroxyl-4'-methoxycinnamate (5). The hypocotyl and root growth of L. sativum were considerably suppressed by these compounds. Methyl (E)-3'-hydroxyl-4'-methoxycinnamate also suppressed the coleoptile and root growth of E. crusgalli. The concentrations of these compounds, causing 50% growth reduction (I50) of L. sativum, were in the range of 74.19–785.4 μM. The findings suggest that these isolated compounds might function in the allelopathy of A. marmelos. [ABSTRACT FROM AUTHOR]

Alzheimer's disease (AD) is the most common neurodegenerative disease that results in memory impairment. Aegle marmelos (L.) Correa (AM) is used as a traditional medicine. AM leaves have the potential to inhibit acetylcholinesterase activity. This study used scopolamine to induce AD in rats. The aim of this study was to investigate the effects of AM leaf extract using this model. Motor and memory functions were tested by the motor activity and Morris water maze (MWM) tests, respectively. The density of the synaptophysin and dendritic spines in the CA1 were detected by immunofluorescence and Golgi impregnation, respectively. The hippocampal histology was reviewed by H&E staining. After the treatment, the latency times in the MWM tests of the AD groups reduced, while the motor activities showed no difference. The density of the synaptophysin of the AD groups increased after the treatments, and that of the dendritic spines also increased in all AD groups post-treatment. The hippocampal tissue also recovered. AM leaf extract can improve cognitive impairment in AD models by maintaining the presynaptic vesicle proteins and dendritic spines in a dose-dependent manner. [ABSTRACT FROM AUTHOR]

The leaves of the Aegle marmelos plant were used for the green synthesis of copper oxide nanoparticles and further characterized by different techniques, including (Ultra Violet-Visible) UV-Vis, Scanning electron microscopy (SEM), Energy dispersive X-ray (EDX), Transmission electron microscopy (TEM) and X-ray diffraction (XRD). The UV-Vis showed a peak at 330 nm, which may be due to the Surface Plasmon Resonance phenomenon. XRD analysis showed the crystalline nature of copper oxide nanoparticles (CuO NPs). In contrast, SEM showed that nanoparticles were not aggregated or clumped, EDX showed the presence of elemental copper., and further, the TEM analysis revealed the average particle size of copper oxide nanoparticles to be 32 nm. The Minimum Inhibitory Concentration (MIC) for Escherichia coli (E. coli) and Staphylococcusaureus (S. aureus) was found to be 400 µg/mL, whereas for Candida albicans (C. albicans) and Candida dubliniensis (C. dubliniensis) it was 800 µg/mL. The zone of inhibition in the well diffusion assay showed the antimicrobial activity of copper oxide nanoparticles, and it also showed that as the concentration of copper oxide nanoparticles increased, the zone of inhibition also increased. Further, the electron microscopic view of the interaction between copper oxide nanoparticles and C. albicans cells showed that CuO NPs were internalized and attached to the cell membrane, which caused changes in the cellular structure and caused deformities which eventually led to cell death. The prepared CuO NPs showed significant photocatalytic degradation of organic dyes in the presence of sunlight. [ABSTRACT FROM AUTHOR]

Apple vinegar is highly recommended for nutrition due to its health benefits and bioactive components. However, the apple cultivar greatly influences the quality of the vinegar. In this research, our focus was on examining the impact of four different apple cultivars on the physicochemical attributes, chemical composition, as well as biological properties—including antidepressant and anti-inflammatory activities—of vinegar. Interestingly, the physicochemical properties of vinegar and the contents of acetic acid and polyphenols depend on the apple cultivars. HPLC chromatographic analysis showed that citric acid (820.62–193.63 mg/100 g) and gallic acid (285.70–54.40 µg/g) were mostly abundant in the vinegar samples. The in vivo results showed that administration of Golden Delicious apple vinegar (10 mL/kg) to adult Wistar rats reduced carrageenan-induced inflammation by 37.50%. The same vinegar sample exhibited a significant antidepressant effect by reducing the rats' immobility time by 31.07% in the forced swimming test. Due to its high acidity, Golden Delicious vinegar was found to be more effective against bacteria, particularly Bacillus subtilis and Candida albicans, resulting in a MIC value of 31.81 mg/mL. Furthermore, the antioxidant activity of various vinegar samples was found to be powerful, displaying optimal values of IC50 = 65.20 mg/mL, 85.83%, and 26.45 AAE/g in the DPPH, β-carotene decolorization and TAC assays, respectively. In conclusion, the apple cultivars used in this study impact the chemical composition and biological activities of vinegar, which may help demonstrate the importance of raw material selection for the production of vinegar. [ABSTRACT FROM AUTHOR]

Krill oil (KO) and the impregnation process to enrich food matrices with bioactive compounds have become relevant. The impregnation process of KO microcapsules into Golden apple tissue was evaluated in this study. A KO emulsion was used at different temperatures to impregnate Golden apple slices. The results show that an increase in temperature causes greater removal of water and penetration of the KO microcapsules. At 60 °C, the volume changes of the samples were evident, obtaining the highest concentration of astaxanthin. Through the impregnation process, it is possible to produce a new food product with potential functional properties. [ABSTRACT FROM AUTHOR]

The aim of the study was to assess the impact of N Pro and Seactiv Complex technologies based on sea algae extracts on the yield and quality of Golden Delicious apple trees compared to the standard fertilization program. Research conducted in the years 2008–2017 showed that the tree fertilization technology and the year of research had a significant impact on the quality and yield parameters. The abundance of seaweed extracts had a positive effect on the greater number of fruits per tree (pcs.), their weight (t × ha−1; kg tree−1), the percentage of fruits exceeding 7.5 cm in size (%) and the average weight of fruits (g) compared to the control combination. In most of the analyzed study years, total yield exceeded the 90 t × threshold regardless of the fertilization program. The use of technology based on sea algae extracts during 10 years of research had a positive effect on the quality and weight compared to the standard fertilization technology commonly used in cultivation. [ABSTRACT FROM AUTHOR]

The conservation of threatened species and the restoration of ecosystems have emerged as crucial ecological prerequisites in the context of a changing global environment. One such species of significant commercial value is the Bael tree, scientifically known as Aegle marmelos, which is native to semi-arid regions in Pakistan. However, the species faces threats in Pakistan due to overexploitation and changing land use. To support sustainable production practices and agricultural planning, it is important to investigate how climate change has affected the geographic distribution of Aegle marmelos. Additionally, the impact of climate change on its frequency and distribution remains uncertain. To address these concerns, we employed species distribution modeling techniques using MaxEnt and GIS to predict the present and future distribution of favorable habitats for Aegle marmelos. Based on our findings, several key bioclimatic variables were identified as significant influencers of Aegle marmelos distribution. These variables include soil bulk density (bdod), isothermality (bio03), precipitation during the warmest quarter (bio18), and mean temperature during the wettest quarter (bio08). Currently, the potential suitable habitat for Aegle marmelos spans an area of approximately 396,869 square kilometers, primarily concentrated in the regions of Punjab, Khyber Pakhtunkhwa, and Balochistan in Pakistan. The habitats deemed highly suitable for Aegle marmelos are predominantly found in upper and central Punjab. However, if climate change persists, the suitable habitats in Pakistan are likely to become more fragmented, resulting in a significant shift in the overall suitable area. Moreover, the distribution center of the species is expected to relocate towards the southeast, leading to increased spatial separation over time. The results of this research significantly contribute to our understanding of the geo-ecological aspects related to Aegle marmelos. Furthermore, they provide valuable recommendations for the protection, management, monitoring, and sustainable production of this species. [ABSTRACT FROM AUTHOR]

Apples represent a category of products frequently consumed by people, owing both to their beneficial effects on human health and to their antioxidant effects. Microwave (MW) treatment is a simple and fast method that can be used successfully in the food industry to obtain dry apple slices, rich in bioactive compounds and with a pleasant aspect. This study presents the effect of applying microwave treating to apple slices from two cultivars (Golden Delicious and Idared), for three, four, and five minutes, respectively, at a power of 450 W, in order to reduce the browning effect. For this purpose, the browning index (BI), chromatic parameters (CIE L*a*b*), total phenolic content (Folin-Ciocâlteu method), and antioxidant capacity (by Ferric Reducing Antioxidant Power (FRAP assay) were evaluated in the case of apple slices before and after MW treatment. Based on the results obtained, it can be argued that the microwave treatment results in a significant increase in the total phenolic content and enhances antioxidant capacity in the case of both apple cultivars. Apples from the Idared cultivar have a higher total phenolic content than apples from the Golden cultivar, and this concentration increased by 56.14% and 48.9%, respectively, after MW treatment. In terms of antioxidant capacity, Idared apples also recorded a higher value compared to Golden cultivars. According to the results of the multivariate analysis, there are variations between the two apple categories with regard to the phenolic content and the browning process; browning was inhibited at the five-minute exposure to microwaves. The apple processing domain may use our findings in order to produce high-quality finished products, with a pleasant aspect, which retain the bioactive compounds of the fruit. [ABSTRACT FROM AUTHOR]

Aegle mamelons (A. marmelos) or Indian Bael leaves possess anti-cancerous and antibacterial properties and are used in the traditional medicine system for the treatment of oral infections. In the present study, the essential oil of the leaves of A. marmelos was explored for its anticancer, antioxidant, and anti-cariogenic properties. The hydro-distilled oil of A. marmelos leaves was analyzed using gas chromatography coupled with mass spectrometry (GC-MS). Monoterpene limonene (63.71%) was found to have the highest percentage after trans-2-Hydroxy-1,8-cineole and p-Menth-2,8-dien-1-ol. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay was used to investigate the anticancer activity of the extracted oil against human oral epidermal carcinoma (KB), and the results showed significantly higher (**** p < 0.0001) anticancer activity (45.89%) in the doxorubicin (47.87%) when compared to the normal control. The antioxidant activity of the essential oil was evaluated using methods of DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)). The results showed a significant (*** p < 0.001) percentage of inhibition of DPPH-induced free radical (70.02 ± 1.6%) and ABTS-induced free radical (70.7 ± 1.32%) at 100 µg/mL with IC50, 72.51 and 67.33 µg/mL, respectively, comparatively lower than standard compound ascorbic acid. The results of the molecular docking study of the significant compound limonene with the receptors tyrosinase and tyrosine kinase 2 supported the in vitro antioxidant potential. The anti-cariogenic activity was evaluated against Streptococcus mutans (S. mutans). Results showed a significant minimum inhibitor concentration of 0.25 mg/mL and the killing time was achieved at 3 to 6 h. The molecular-docking study showed that limonene inhibits the surface receptors of the S. mutans c-terminal domain and CviR protein. The study found that A. marmelos leaves have potential anti-carcinoma, antioxidant, and anti-cariogenic effects on human oral epidermal health, making them a valuable natural therapeutic agent for managing oral cancer and infections. [ABSTRACT FROM AUTHOR]

This work aims to estimate the shelf life of Golden Delicious apple fruit stored at room temperature by determining the changing trend in color every week using a non-destructive measurement method. Moreover, the study will measure the changes in weight loss (Δm) and the total soluble solids (TSS) contained in the apple. The research focuses on the last stage of ripening and the effect of shelf life affecting consumer behavior; therefore, the examined fruits were picked at the end of the season and were stored at an ambient temperature and in controlled laboratory conditions for six weeks, at 24 °C under 60% RH relative. Color measurements were performed with a portable color sensor, which provided a simple and effective examination method in the case of an appropriate number of fruit samples. The findings showed a significant increase in TSS and weight loss over time. Color varying (ΔE) and chroma (C*) parameters increased with prolonged storage duration, meaning that the color of the apples became darker and more color-saturated at the end of storage. While weight loss and TSS follow a linear tendency in the given storage period, the color changes in deteriorating fruits were characterized by exponential asymptotic regression. It was found that although the moisture content reaches its limit value during the drying process of apples, the saturation of color coordinates allows for determination of the limit parameters of consumption in the linear stage of weight loss. The saturation limits (70.97; 12.77; 56.34 CIELAB L*; a*; b*), the dominant color part (b*), and the growth rate of the saturation curves allow an accurate characterization of ripening from the visual aspect, thus determining the limit parameters of shelf life and improving the critical analysis factors affecting the life of fruits after harvest. By assessing color characteristics using a non-destructive technology, customers may quickly evaluate the quality of apples and make better decisions during their purchase. [ABSTRACT FROM AUTHOR]

Obesity and overweight have serious health outcomes. "Phikud Tri-Phon" (PTP) is a traditional Thai medicine comprising three dried fruits from Aegle marmelos L., Morinda citrifolia L., and Coriandrum sativum L. Whether this medicine impacts on metabolic disease is unclear. This study aimed to investigate the phenolic and flavonoid contents of PTP and each of its herbal components, and further assess their antioxidant and anti-adipogenetic activities. Oil-red O staining was measured for lipid accumulation in 3T3-L1 adipocytes. The chemical profiles of PTP and each herbal extract were determined by LC-ESI-QTOF-MS/MS. Our results show that the total phenolic and flavonoid contents of PTP water extract were 22.35–108.42 mg of gallic acid equivalents and PTP ethanolic extract was 1.19–0.93 mg of quercetin equivalents and the DPPH scavenging capacity assay of PTP ethanolic extract (1 mg/mL) was 92.45 ± 6.58 (Trolox equivalent)/g. The PTP extracts and individual herbs had inhibitory adipogenesis activity, which reduced lipid accumulation by approximately 31% in PTP water extract and 22% in PTP ethanolic extract compared with control cells. These results provided insights into the traditional preparation method of using boiling water as a vehicle for PTP. In conclusion, PTP has antioxidant and anti-adipogenesis potential, indicating it is a promising ingredient in functional food and herbal health products. [ABSTRACT FROM AUTHOR]

Aegle marmelos (L.) Correa (Bael) fruit, a member of the Rutaceae family, is a major cultivated fruit plant in tropical and subtropical regions in countries of southeast Asia. Bael fruit has been a major topic for studies in recent years mainly due to its high nutritional (carbohydrates, proteins, minerals, and vitamins) value and presence of various phytochemicals, which attributed to its high medicinal value. These phytochemicals include various compounds, e.g., alkaloids, flavonoids, and phenolic acids (protocatechuic acid, gallic, and ellagic acid). The fruit extract of bael has been also an important study area for its pharmacological activities, including antidiarrheal, antioxidant, antidiabetic, hepatoprotective, radioprotective, anticancer, antiulcer properties. The current review mainly highlighted the nutritional and pharmacological activities of bael fruit. The nutritional profile and phytochemical profile were discussed in the review, along with their concentration in the fruit. Moreover, the experiments carried out in vivo and in vitro of bael fruit extracts with respect to their pharmacological activities were also discussed in the article. The recent literature based on nutritional and pharmacological values of bael fruit showed its high potential as a food and pharmaceutical product. Despite having high nutritional and pharmacological value, research related to molecular mechanisms of bael fruit is still limited, and clinical trials are needed to ensure its safety as a product in the food and pharma industries. [ABSTRACT FROM AUTHOR]

This paper presents a simple method of obtaining a bacterial cellulose (BC) composite with the addition of graphene oxide (GO) using an in situ method and studies the influence of GO nanoparticles on the structure and properties of the obtained membranes. Microorganisms obtained from Golden Delicious apple vinegar were used to obtain the BC. During the biosynthesis, GO was introduced in the amounts of 3.7, 5.4 and 7.1% w/w. The resulting BC/GO composite was characterized by high water content (~400%), a thickness of about 1.1 mm (in wet form) and a cellulose nanofiber diameter of ~100 nm. The possibility of using the resulting composite membranes as potential active dressings with the sustained-release analgesic medicine—paracetamol—was investigated. The BC/GO composite membranes were characterized by a medicine sorption of 60 mg/g of BC, a slow desorption time, a constant medicine concentration over time and an 80% paracetamol release rate after 24 h. The morphology of membrane surfaces and cross-sections were examined by means of scanning electron microscopy (SEM). Infrared spectroscopy (FTIR), X-ray structure studies (WAXS) as well as thermal analysis (TGA) demonstrated the presence of GO in the BC matrix and interactions between the matrix and the additive. [ABSTRACT FROM AUTHOR]

Apples are easily damaged during transportation due to extrusion and collision, resulting in structural damage and deterioration. To better understand apples' mechanical–structural damage behavior, a texture analyzer platform combined with in situ observation was established. The effects of extrusion distance, speed, working temperature, and typical kinds of apple were considered for damage mechanisms. Apple damage was analyzed via the finite element method (FEM). The results indicated that the apple extrusion behavior can be divided into elastic interaction and plastic damage. Compression displacement effects were obviously significant in terms of structural damage, and apple samples were in an elastic stage with displacement of less than 2.3 mm, and no structural damage. The peak force energy-displacement mathematical model was established, showing an "s" shape and upward parabolic shape. The critical compression energy was around 100N·mm during elastic interaction. The damaged area was positively correlated with the compression energy. The FEM simulation results were consistent with the damage distribution of apples. The effects of speed on the three apple types were different. Red Fuji apples with a bruised area were not sensitive to pressure speed. The effect on the crack forming of Ralls apples was significant. Golden Delicious apples with a bruised area and crack formation showed an intermediate effect. The peak force–temperature fitting curve showed a downward parabolic shape and an R2 determination factor of 0.99982. Apple squeeze damage mechanisms provide theoretical guidance for apple damage control. [ABSTRACT FROM AUTHOR]

The (Forests) Editorial Office wishes to make the following changes to the author's paper [[1]].Affiliation Update:In the publication, there was an error regarding the affiliation for "Prashant Kaushik". The updated affiliation should be: "Independent Researcher, 46022 Valencia, Spain". And the email address should be "prashantumri@gmail.com".The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.By Prashant Kaushik and Shashi KumarReported by Author; Author [Extracted from the article]

There are many methods to detect plant pests and diseases, but they are primarily time-consuming and costly. Computer vision techniques can recognize the pest- and disease-damaged fruits and provide clues to identify and treat the diseases and pests in their early stages. This study aimed to identify common pests, including the apple capsid (Plesiocoris rugicollis)/AC, apple codling moth (Cydia pomonella)/ACM, Pear lace bug (Stephanitis pyri)/PLB, and one physiological disease-apple russeting/AR in two cultivars, Golden Delicious and Red Delicious, using the digital image processing and sparse coding method. The Sparse coding method is used to reduce the storage of the elements of images so that the matrix can be processed faster. There have been numerous studies on the identification of apple fruit diseases and pests. However, most of the previous studies focused only on diagnosing a pest or disease, not on computational volume reduction and rapid detection. This research focused on the comprehensive study on identifying pests and diseases of apple fruit using sparse coding. The sparse coding algorithm in this work was designed using Matlab software. The apple pest and disease detection were performed based on 11 characteristics: R, G, B, L, a, b, H, S, V, Sift, and Harris. The class detection accuracy using the sparse coding method was obtained for 10 classes with three views of apple for S. pyri of red apple as 81%, S. pyri of golden apple as 88%, golden apple russeting as 85%, S. pyri and russeting of red apple as 100%, S. pyri and russeting of golden apple as 80%, codling moth of red apple as 86%, codling moth of golden apple as 72%, S. pyri of red apple as 83%, S. pyri of golden apple as 90%, codling moth and S. pyri of red apple as 80%, and codling moth and S. pyri of golden apple as 67%. The total processing time for developing the dictionary was 220 s. Once the dictionary was developed, pest and disease detection took only 0.175 s. The results of this study can be useful in developing automatic devices for the early detection of common pests and diseases of apples. Although the study was focused on apple diseases, results for this work have huge potential for other crops. [ABSTRACT FROM AUTHOR]

Recipes for Thai traditional herbal teas have been formulated based on Thai traditional medicine, which tries to achieve a balance of the body's elements (fire, water, wind and earth) in each season. This study aims to assess the benefits of Thai traditional herbal teas through evaluating their antioxidant properties and measuring the satisfaction levels of healthy volunteers. Antioxidant activity tests performed on 11 tea recipes and on green tea (positive control) included 2,2-diphenyl-1-picrylhydrazyl (DPPH), a radical scavenging assay and a 2,2'-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) assay. In addition, total phenolic contents were determined using a Folin-Ciocalteu assay. Tests of satisfaction were performed on volunteer subjects aged between 18 and 30 years old, using the criteria of color, flavor, taste and overall satisfaction. Among the 11 tea recipes tested, the summer recipe 1 containing Aegle marmelos (L.) Correa, Stevia rebaudiana (Bert.) Bertoni, Pandanus amaryllifolius Roxb. and Morus alba L. demonstrated the most promising antioxidant properties (p > 0.05) and a phenolic content (p > 0.05), which was comparable to green tea. Furthermore, it got the highest satisfaction scores in every assessed category. Therefore, this study shows that Thai traditional herbal teas, developed based on the concepts of Thai traditional medicine, could be a good choice for people looking for health-promoting beverages. [ABSTRACT FROM AUTHOR]

The role of melatonin during the growth and ripening of apple fruit was studied using local varieties. The evolution of the growth and ripening parameters, including fruit size and weight, firmness, color change, sugar content, and ethylene production, was different in the five varieties studied, with yellow apples (Reineta and Golden) initiating the ripening process earlier than reddish ones (Teórica, Sanroqueña, and Caguleira). Changes in the melatonin and melatonin isomer 2 contents during growth and ripening were studied in Golden apples, as was the effect of the melatonin treatment (500 µM, day 124 post-anthesis) on the apple tree. Melatonin content varied greatly, with higher value in the skin than in the flesh. In the skin, melatonin increased at day 132 post-anthesis, when ethylene synthesis started. In the flesh, melatonin levels were high at the beginning of the growth phase and at the end of ripening. Melatonin isomer 2 was also higher once the ripening started and when ethylene began to increase. The melatonin treatment significantly advanced the ethylene production and increased the fruit size, weight, sugar content, and firmness. The data suggest that melatonin stimulates fruit ripening through the induction of ethylene synthesis, while melatonin treatments before ripening improve the final fruit quality. [ABSTRACT FROM AUTHOR]

For many years, Aegle marmelos (A. marmelos) has been used medicinally and as a dietary supplement. Despite this, there are minimal research data on A. marmelos phytochemical properties and pharmacological effects. This study aimed to explore the phytoconstituents, cytotoxicity, glucose uptake, and antioxidant and antidiabetic potential of an alcoholic extract of A. marmelos leaf. The cytotoxicity of A. marmelos in HepG2 cells was tested in vitro, and the results revealed that it has strong cytocompatibility and cytoprotective properties. The extract's antioxidant activities were investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. Antioxidant potential was shown to be quite impressive. The enzymes α-amylase and α-glycosidase were found to be substantially inhibited by A. marmelos, with IC50 values of 46.21 and 42.07 mg/mL, respectively. In HepG2 cells, A. marmelos significantly reduced ROS levels that were elevated due to high glucose and enhanced glucose consumption (p < 0.05). These activities might be due to the enrichment of bioactive phytoconstituents analyzed chromatographically using GC/MS and HPLC. The findings of this study show that A. marmelos could be an effective restorative therapy for diabetes and related diseases. [ABSTRACT FROM AUTHOR]

The Pikad Tri-phol-sa-mut-than (TS) remedy, a Thai traditional medicine, is officially recorded in Tamra Paetsart Sonkrau Chabub Anurak for its capabilities in treating kidney deficiency. TS remedy is composed of three fruit species—Aegle marmelos (L.) Corrêa., Coriandrum sativum L., and Morinda citrifolia L.—in an equal part by weight. The quality of the raw material is one of the essential factors that can affect the effectiveness and safety of treatment by herbal remedy. The pharmacognostic evaluation and DNA barcode of the three fruit species and TS remedy were performed in this study to authenticate them from contamination, and to provide the scientific database for further uses. Macroscopic and microscopic examination, chemical profile by TLC, and DNA barcoding were employed to positively identify the raw materials bought from the herbal market, especially the powder form. Consequently, the outcomes of this investigation can be used to develop an essential and effective tool for the authentication of crude drugs and herbal remedies. [ABSTRACT FROM AUTHOR]

The current study explores the methanolic extracts of the leaves and fruit of Aegle marmelos (Bael) for their total phenolic content (TPC), total flavonoids content (TFC), antioxidants, and antibiofilms, as well as its in ovo antiviral potential against Newcastle disease virus (NDV). The drug-likeliness thereof and the potential identification of an interaction—their molecular docking of ligands with target proteins by GOLD—was determined in silico using the Swiss ADME software. The total flavonoids content (TFC) was 135.17 ± 2.02 and 111.2 ± 3.67 mg QE/g, while the total phenolics content (TPC) was 185.02 ± 2.15 and 171.13 ± 6.73 mg GAE/g, in the fruit and leaves extracts, respectively. In a DPPH assay, the IC50 value for the methanolic extracts of leaves and fruit was 63.52 ± 1.48 and 52.06 ± 1.62. μg/mL d.w. The fruit extract of A. marmelos showed significantly higher reducing power (i.e., 59.32 ± 0.05 µmol/g d.w) than the leaves extract (p < 0.05). The biofilm-inhibition activity of the fruit extract of A. marmelos was 65.78 ± 0.65 µg/mL. Both parts of the plant showed potent antiviral potential at higher concentrations. A study in silico, using the molecular docking of three compounds, showed good interaction with the HN protein, with considerable binding affinities and fulfilled docking parameters. This work shows that Aegle marmelos and its phytoconstituents can be used as a potential remedy for NDV. [ABSTRACT FROM AUTHOR]

The development of textile finishing with improved functional properties has been a growing interest among industry and scientists worldwide. The recent global pandemic also enhanced the awareness amongst many toward improved hygiene and the use of antimicrobial textiles. Generally, natural herbal components are known to possess antimicrobial properties which are green and eco-friendly. This research reports a novel and innovative method of developing and optimising nano-emulsions using two combinations of herbal extracts produced from Moringa Oleifera, curry leaf, coconut oil (nano-emulsion 1) and other using Aegle marmelos with curry leaf and coconut oil (nano-emulsion 2). Nano-emulsions were optimised for their pH, thermal stability, and particle size, and percentage add-on. Organic cotton fabrics (20 and 60 gsm) were finished with nano-emulsions using continuous and batch processes and characterised for their surface morphology using scanning electron microscopy, energy dispersive X-ray (EDX) analysis and Fourier transform infrared spectroscopy (FTIR) analysis. The finished fabrics were evaluated for their Whiteness Index, assessed for antimicrobial resistance against Gram-positive (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) using AATCC 100 and 147 methods. In addition, fabrics were assessed for their antifungal efficacy (AATCC 30), tensile strength and air permeability. Results suggested that finished organic fabrics with nano-emulsions had antimicrobial resistance, antifungal, wash fastness after 20 washing cycles, and sufficient strength. This novel finishing method suggests that organic cotton fabrics treated with nano-emulsions can be used as a durable antimicrobial textile for healthcare and hygiene textiles. [ABSTRACT FROM AUTHOR]

Four different varieties of apples have been considered (Limoncella, Annurca, Red Delicious, and Golden Delicious) to estimate the extent of colon polyphenolics release after in vitro sequential enzyme digestion. Since several studies report a positive effect of apple polyphenols in colonic damage, we found of interest to investigate the colon release of polyphenols in different varieties of apples in order to assess their prevention of colonic damage. UHPLC-HRMS analysis and antioxidant activity (ABTS, DPPH, and FRAP assays) were carried out on the apple extracts (peel, flesh, and whole fruit) obtained from not digested samples and on bioaccessible fractions (duodenal and colon bioaccessible fractions) after in vitro digestion. Polyphenolic content and antioxidant activities were found to vary significantly among the tested cultivars with Limoncella showing the highest polyphenol content accompanied by an excellent antioxidant activity in both flesh and whole fruit. The overall trend of soluble antioxidant capacity from the soluble duodenal phase (SDP) and soluble colonic phase (SCP) followed the concentrations of flavanols, procyandinis, and hydroxycinnamic acids under the same digestive steps. Our results highlighted that on average 64.2% of the total soluble antioxidant activity was released in the SCP with Limoncella exhibiting the highest values (82.31, 70.05, and 65.5%, respectively for whole fruit, flesh, and peel). This result suggested that enzymatic treatment with pronase E and viscozyme L, to reproduce biochemical conditions occurring in the colon, is effective for breaking the dietary fiber-polyphenols interactions and for the release of polyphenols which can exercise their beneficial effects in the colon. The beneficial effects related to the Limoncella consumption could thus be of potential great relevance to counteract the adverse effects of pro-oxidant and inflammatory processes on intestinal cells. [ABSTRACT FROM AUTHOR]

The application of coatings with essential oils for food preservation is an alternative way to keep minimally processed apple slices fresh, nutritious, safe, sensory palatable, and accessible for consumers. In the present study, the effect of three bioactive coatings on quality variables of minimally processed Golden Delicious apple slices for 25-days at 4 °C was evaluated. The coatings were CT1-chitosan-based, CT2-guar gum-based, and CT3-composite guar gum-starch-based; all three coatings contained cinnamon essential oil and were compared with UCT0-uncoated apple slices. The quality variables evaluated were weight-loss, firmness, browning index, total phenolic content, total soluble solids, titratable acidity, respiration rate, microbial analysis, and sensory evaluation. All coatings improved the preservation and sensorial quality variables of Golden Delicious apples; however, although the CT1-chitosan-based coating was capable of extending the shelf-life of minimally processed apple, it demonstrated less sensorially favorable scores for flavor, odor, and overall acceptance attributes. [ABSTRACT FROM AUTHOR]

The cuticle, a protective cuticular barrier present in almost all primary aerial plant organs, has a composition that varies between plant species. As a part of the apple peel, cuticle and epicuticular waxes have an important role in the skin appearance and quality characteristic in fresh fruits destined for human consumption. The specific composition and structural characteristics of cutin from two apple varieties, "golden delicious" and "red delicious", were obtained by enzymatic protocols and studied by means of cross polarization magic angle spinning nuclear magnetic resonance (CP-MAS 13C NMR), attenuated total reflection infrared spectroscopy (ATR-FTIR), and mass spectrometry, and were morphologically characterized by specialized microscopy techniques (atomic force microscopy (AFM), confocal laser scanning microscopy (CLMS), and scanning electron microscopy (SEM)). According to CP-MAS 13C NMR and ATR-FTIR analysis, cutins from both varieties are mainly composed of aliphatics and a small difference is shown between them. This was corroborated from the hydrolyzed cutins analysis by mass spectrometry, where 9,10,18-trihydroxy-octadecanoic acid; 10,20-Dihydroxy-icosanoic acid; 10,16-dihydroxy hexadecenoic acid (10,16-DHPA); 9,10-epoxy-12-octadecenoic acid; and 9,10-epoxy-18-hydroxy-12-octadecenoic acid were the main monomers isolated. The low presence of polysaccharides and phenolics in the cutins obtained could be related to the low elastic behavior of this biocomposite and the presence of cracks in the apple cutin's surface. These cracks have an average depth of 1.57 µm ± 0.57 in the golden apple, and 1.77 µm ± 0.64 in those found in the red apple. The results obtained in this work may facilitate a better understanding that mechanical properties of the apple fruit skin are mainly related to the specific aliphatic composition of cutin and help to much better investigate the formation of microcracks, an important symptom of russet formation. [ABSTRACT FROM AUTHOR]

Different polyvinyl alcohol (PVA) coating formulations incorporating starch (S) and carvacrol (C) as the active agent were applied to Golden Delicious apples to evaluate their effectiveness at controlling weight loss, respiration rate, fruit firmness, and fungal decay against B. cinerea and P. expansum throughout storage time. Moreover, the impact of these coatings on the sensory attributes of the fruit was also analyzed. The application of the coatings did not notably affect the weight loss, firmness changes, or respiration pathway of apples, probably due to the low solid surface density of the coatings. Nevertheless, they exhibited a highly efficient disease control against both black and green mold growths, as a function of the carvacrol content and distribution in the films. The sensory analysis revealed the great persistence of the carvacrol aroma and flavor in the coated samples, which negatively impact the acceptability of the coated products. [ABSTRACT FROM AUTHOR]

Neglected and underutilized fruit species (NUFS) can make an important contribution to the economy, food security and nutrition requirement for Sri Lanka. Identifying suitable areas for cultivation of NUFS is of paramount importance to deal with impending climate change issues. Nevertheless, limited studies have been carried out to assess the impact of climate change on the potential distribution of NUFS. Therefore, we examined the potential range changes of NUFS in a tropical climate using a case study from Sri Lanka. We prioritized and modeled the potentially suitable areas for four NUFS, namely Aegle marmelos, Annona muricata, Limonia acidissima and Tamarindus indica under current and projected climates (RCP 4.5 and RCP 8.5) for 2050 and 2070 using the maximum entropy (Maxent) species distribution modeling (SDM) approach. Potentially suitable areas for NUFS are predicted to decrease in the future under both scenarios. Out of the four NUFS, T. indica appears to be at the highest risk due to reduction in potential areas that are suitable for its growth under both emissions scenarios. The predicted suitable area reductions of this species for 2050 and 2070 are estimated as >75% compared to the current climate. A region of potentially higher climatic suitability was found around mid-county for multiple NUFS, which is also predicted to decrease under projected climate change. Further, the study identified high-potential agro-ecological regions (AERs) located in the mid-country's wet and intermediate zones as the most suitable areas for promoting the cultivation of NUFS. The findings show the potential for incorporating predictive modeling into the management of NUFS under projected climate change. This study highlights the requirements of climate change adaptation strategies and focused research that can increase the resilience of NUFS to future changes in climate. [ABSTRACT FROM AUTHOR]

Aegle marmelos (L.) Corr. is a medicinally and horticulturally important tree member of the family Rutaceae. It is native to India, where it is also known as Bael. Despite its importance, the genomic resources of this plant are scarce. This study presented the first-ever report of expressed transcripts in the leaves of Aegle marmelos. A total of 133,616 contigs were assembled to 46,335 unigenes with minimum and maximum lengths of 201 bp and 14,853 bp, respectively. There were 7002 transcription factors and 94,479 simple sequence repeat (SSR) markers. The A. marmelos transcripts were also annotated based on information from other members of Rutaceae; namely Citrus clementina and Citrus sinensis. A total of 482 transcripts were annotated as cytochrome p450s (CYPs), and 314 transcripts were annotated as glucosyltransferases (GTs). In the A. marmelos leaves, the monoterpenoid biosynthesis pathway was predominant. This study provides an important genomic resource along with useful information about A. marmelos. [ABSTRACT FROM AUTHOR]