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Your search keyword '"Feng Liu"' showing total 4 results
Start Over Author "Feng Liu" Topic transcription factors Publisher mdpi
4 results on '"Feng Liu"'

1. Genome-Wide Identification and Characterization of the Mitochondrial Transcription Termination Factors (mTERFs) in Capsicum annuum L

Author

Xiang Cheng, Junheng Lv, Ting Yi, Xie Lingling, Zou Xuexiao, Feng Liu, Huiping Yang, and Bingqian Tang

Subjects
0106 biological sciences, 0301 basic medicine, Mitochondrial DNA, abiotic stress, Biology, Mitochondrion, 01 natural sciences, Genome, Catalysis, Article, lcsh:Chemistry, Inorganic Chemistry, Mitochondrial Proteins, 03 medical and health sciences, Gene family, expression analysis, Physical and Theoretical Chemistry, lcsh:QH301-705.5, Molecular Biology, Gene, Spectroscopy, Plant Proteins, Genetics, GO analysis, Abiotic stress, phylogenetic analysis, Organic Chemistry, food and beverages, Promoter, General Medicine, mTERF gene family, Computer Science Applications, Mitochondria, Gene Expression Regulation, Neoplastic, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, capsicum, Function (biology), 010606 plant biology & botany, Genome-Wide Association Study, Transcription Factors
Abstract

Mitochondrial transcription termination factors (mTERFs) regulate the expression of mitochondrial genes and are closely related to the function of the mitochondrion and chloroplast. In this study, the mTERF gene family in capsicum (Capsicum annuum L.) was identified and characterized through genomic and bioinformatic analyses. Capsicum was found to possess at least 35 mTERF genes (CamTERFs), which were divided into eight major groups following phylogenetic analysis. Analysis of CamTERF promoters revealed the presence of many cis-elements related to the regulation of cellular respiration and photosynthesis. In addition, CamTERF promoters contained cis-elements related to phytohormone regulation and stress responses. Differentially expressed genes in different tissues and developmental phases were identified using RNA-seq data, which revealed that CamTERFs exhibit various expression and co-expression patterns. Gene ontology (GO) annotations associated CamTERFs primarily with mitochondrion and chloroplast function and composition. These results contribute towards understanding the role of mTERFs in capsicum growth, development, and stress responses. Moreover, our data assist in the identification of CamTERFs with important functions, which opens avenues for future studies.

Published
2019

2. Expression Characteristics and Functional Analysis of the ScWRKY3 Gene from Sugarcane

Author

Ming-Jian Dai, Feng Liu, Youxiong Que, Yachun Su, Liping Xu, Yufeng Chen, Shengxiao Yu, Wenju Wang, Tingting Sun, Ling Wang, and Xu Zhang

Subjects
0106 biological sciences, 0301 basic medicine, Nicotiana benthamiana, Acetates, Sodium Chloride, 01 natural sciences, Polyethylene Glycols, lcsh:Chemistry, protein-protein interaction, chemistry.chemical_compound, Gene Expression Regulation, Plant, sugarcane, subcellular localization, lcsh:QH301-705.5, Abscisic acid, Spectroscopy, Phylogeny, Plant Proteins, Ralstonia solanacearum, Methyl jasmonate, biology, food and beverages, General Medicine, Computer Science Applications, Saccharum, Organ Specificity, Plant hormone, Salicylic Acid, Cyclopentanes, Catalysis, Article, Microbiology, Inorganic Chemistry, 03 medical and health sciences, Stress, Physiological, Oxylipins, Physical and Theoretical Chemistry, Molecular Biology, transient overexpression, Plant Diseases, Cell Nucleus, Organic Chemistry, fungi, WRKY, biology.organism_classification, WRKY protein domain, gene expression pattern, Plant Leaves, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, chemistry, Smut, 010606 plant biology & botany, Abscisic Acid, Transcription Factors
Abstract

The plant-specific WRKY transcriptional regulatory factors have been proven to play vital roles in plant growth, development, and responses to biotic and abiotic stresses. However, there are few studies on the WRKY gene family in sugarcane (Saccharum spp.). In the present study, the characterization of a new subgroup, IIc WRKY protein ScWRKY3, from a Saccharum hybrid cultivar is reported. The ScWRKY3 protein was localized in the nucleus of Nicotiana benthamiana leaves and showed no transcriptional activation activity and no toxic effects on the yeast strain Y2HGold. An interaction between ScWRKY3 and a reported sugarcane protein ScWRKY4, was confirmed in the nucleus. The ScWRKY3 gene had the highest expression level in sugarcane stem pith. The transcript of ScWRKY3 was stable in the smut-resistant Saccharum hybrid cultivar Yacheng05-179, while it was down-regulated in the smut-susceptible Saccharum hybrid cultivar ROC22 during inoculation with the smut pathogen (Sporisorium scitamineum) at 0⁻72 h. ScWRKY3 was remarkably up-regulated by sodium chloride (NaCl), polyethylene glycol (PEG), and plant hormone abscisic acid (ABA), but it was down-regulated by salicylic acid (SA) and methyl jasmonate (MeJA). Moreover, transient overexpression of the ScWRKY3 gene in N. benthamiana indicated a negative regulation during challenges with the fungal pathogen Fusarium solani var. coeruleum or the bacterial pathogen Ralstonia solanacearum in N. benthamiana. The findings of the present study should accelerate future research on the identification and functional characterization of the WRKY family in sugarcane.

Published
2018

3. Transcriptomic Analysis of Phenotypic Changes in Birch (Betula platyphylla) Autotetraploids.

Author

Huai-Zhi Mu, Zi-Jia Liu, Lin, Lin, Hui-Yu Li, Jing Jiang, and Gui-Feng Liu

Subjects
TRANSCRIPTION factors, PHENOTYPES, PLANT chromosomes, AUTOTETRAPLOIDY, BIRCH, PLANT breeders, FORESTS & forestry, GENE expression in plants
Abstract

Plant breeders have focused much attention on polyploid trees because of their importance to forestry. To evaluate the impact of intraspecies genome duplication on the transcriptome, a series of Betula platyphylla autotetraploids and diploids were generated from four full-sib families. The phenotypes and transcriptomes of these autotetraploid individuals were compared with those of diploid trees. Autotetraploids were generally superior in breast-height diameter, volume, leaf, fruit and stoma and were generally inferior in height compared to diploids. Transcriptome data revealed numerous changes in gene expression attributable to autotetraploidization, which resulted in the upregulation of 7052 unigenes and the downregulation of 3658 unigenes. Pathway analysis revealed that the biosynthesis and signal transduction of indoleacetate (IAA) and ethylene were altered after genome duplication, which may have contributed to phenotypic changes. These results shed light on variations in birch autotetraploidization and help identify important genes for the genetic engineering of birch trees. [ABSTRACT FROM AUTHOR]

Published
2012
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4. Genome-Wide Analysis of a TaLEA-Introduced Transgenic Populus simonii x Populus nigra Dwarf Mutant.

Author

Hong-Mei Yuan, Su Chen, Lin Lin, Rui Wei, Hui-Yu Li, Gui-Feng Liu, and Jing Jiang

Subjects
BLACK poplar, AGROBACTERIUM tumefaciens, CHROMOSOMES, TRANSCRIPTION factors, POLYMERASE chain reaction
Abstract

A dwarf mutant (dwf1) was obtained among 15 transgenic lines, when TaLEA (Tamarix androssowii late embryogenesis abundant gene) was introduced into Populus simonii x Populus nigra by Agrobacterium tumefaciens-mediated transformation. Under the same growth conditions, dwf1 height was significantly reduced compared with the wild type and the other transgenic lines. Because only one transgenic line (dwf1) displayed the dwarf phenotype, we considered that T-DNA insertion sites may play a role in the mutant formation. The mechanisms underlying this effect were investigated using TAIL-PCR (thermal asymmetric interlaced PCR) and microarrays methods. According to the TAIL-PCR results, two flanking sequences located on chromosome IV and VIII respectively, were cloned. The results indicated the integration of two independent T-DNA copies. We searched for the potential genes near to the T-DNA insertions. The nearest gene was a putative poplar AP2 transcription factor (GI: 224073210). Expression analysis showed that AP2 was up-regulated in dwf1 compared with the wild type and the other transgenic lines. According to the microarrays results, a total of 537 genes involved in hydrolase, kinase and transcription factor activities, as well as protein and nucleotide binding, showed significant alterations in gene expression. These genes were expressed in more than 60 metabolic pathways, including starch, sucrose, galactose and glycerolipid metabolism and phenylpropanoids and flavonoid biosyntheses. Our transcriptome and T-DNA insertion sites analyses might provide some useful insights into the dwarf mutant formation. [ABSTRACT FROM AUTHOR]

Published
2012
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