1. The Urinary Transcriptome as a Source of Biomarkers for Prostate Cancer.
- Author
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Solé, Carla, Goicoechea, Ibai, Goñi, Alai, Schramm, Maike, Armesto, María, Arestin, María, Manterola, Lorea, Tellaetxe, Maitena, Alberdi, Aitor, Nogueira, Leonor, Roumiguie, Mathieu, López, Jose Ignacio, Sanz Jaka, Juan Pablo, Urruticoechea, Ander, Vergara, Itziar, Loizaga-Iriarte, Ana, Unda, Miguel, Carracedo, Arkaitz, Malavaud, Bernard, and Lawrie, Charles H.
- Subjects
BIOMARKERS ,BLADDER ,CENTRIFUGATION ,POLYMERASE chain reaction ,PROSTATE tumors ,BENIGN prostatic hyperplasia ,PROSTATE-specific antigen ,GENE expression profiling ,EARLY detection of cancer - Abstract
Prostate cancer (PCa) is the second most common cancer of men and is typically slow-growing and asymptomatic. The use of blood PSA as a screening method has greatly improved PCa diagnosis, but high levels of false positives has raised much interest in alternative biomarkers. We used next-generation sequencing (NGS) to elucidate the urinary transcriptome of whole urine collected from high-stage and low-stage PCa patients as well as from patients with the confounding diagnosis of benign hyperplasia (BPH). We identified and validated five differentially expressed protein-coding genes (FTH1 BRPF1, OSBP, PHC3, and UACA) in an independent validation cohort of small-volume (1 mL) centrifuged urine (n = 94) and non-centrifuged urine (n = 84) by droplet digital (dd)PCR. These biomarkers were able to discriminate between BPH and PCa patients and healthy controls using either centrifuged or non-centrifuged whole urine samples, suggesting that the urinary transcriptome is a valuable source of non-invasive biomarkers for PCa that warrants further investigation. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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