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The Fruit Harvest Helper, a mobile application developed by Northwest Nazarene University's (NNU) Robotics Vision Lab, aims to assist farmers in estimating fruit yield for apple orchards. Currently, farmers manually estimate the fruit yield for an orchard, which is a laborious task. The Fruit Harvest Helper seeks to simplify their process by detecting apples on images of apple trees. Once the number of apples is detected, a correlation can then be applied to this value to obtain a usable yield estimate for an apple tree. While prior research efforts at NNU concentrated on developing an iOS app for blossom detection, this current research aims to adapt that smart farming application for apple detection across multiple platforms, iOS and Android. Borrowing ideas from the former iOS app, the new application was designed with an intuitive user interface that is easy for farmers to use, allowing for quick image selection and processing. Unlike before, the adapted app uses a color ratio-based image-segmentation algorithm written in C++ to detect apples. This algorithm detects apples within apple tree images that farmers select for processing by using OpenCV functions and C++ code. The results of testing the algorithm on a dataset of images indicate an 8.52% Mean Absolute Percentage Error (MAPE) and a Pearson correlation coefficient of 0.6 between detected and actual apples on the trees. These findings were obtained by evaluating the images from both the east and west sides of the trees, which was the best method to reduce the error of this algorithm. The algorithm's processing time was tested for Android and iOS, yielding an average performance of 1.16 s on Android and 0.14 s on iOS. Although the Fruit Harvest Helper shows promise, there are many opportunities for improvement. These opportunities include exploring alternative machine-learning approaches for apple detection, conducting real-world testing without any human assistance, and expanding the app to detect various types of fruit. The Fruit Harvest Helper mobile application is among the many mobile applications contributing to precision agriculture. The app is nearing readiness for farmers to use for the purpose of yield monitoring and farm management within Pink Lady apple orchards. [ABSTRACT FROM AUTHOR]

In recent years, an increase in the frequency of low-sunlight conditions due to climate change has resulted in a decline in the yield and quality of crops for greenhouse farmers, leading to significant challenges in maintaining optimal plant growth. The crop growth model can be used to predict changes in cucumber yield in response to variations in sunlight, which can help efficiently address sunlight shortages. The objective of this study was to improve and validate the model for predicting cucumber yield under different light environment conditions, including shading and supplemental lighting. The model comprises three steps: LAI prediction, daily assimilate yield prediction, and fruit yield prediction, each of which involves modifying the coefficients applied to suit the cucumber cultivar and environment condition. The improved model demonstrated a high degree of accuracy in predicting cucumber yields in the control and low-sunlight treatments (10, 20, and 30% shading), with a coefficient of determination (R2) > 0.98. When supplemental lighting was incorporated into the control and shading treatments, the accuracy of the improved model in predicting cucumber yield was also high, with a coefficient of determination (R2) > 0.99. The model also accurately predicted the decrease in cucumber fruit yield under low-sunlight conditions (shading treatments) and the increase in yield due to supplemental lighting. The findings of this study indicate that the improved cucumber yield prediction model can be applied to assess the efficacy of yield reduction in low-sunlight conditions and the potential for yield enhancement through supplemental lighting. [ABSTRACT FROM AUTHOR]

Optimal fruit production from many tree crops relies on the transfer of cross-pollen between trees of different cultivars rather than the transfer of self-pollen between trees of the same cultivar. However, many orchards are established with wide blocks of single cultivars, which can result in high percentages of self-fertilised fruit and sub-optimal yield and quality. We aimed to determine whether outcrossing rates and yield of Hass avocado fruit decline with increasing distance from polliniser trees of cultivar Shepard and whether selfed fruit are smaller than outcrossed fruit. Outcrossing rates declined from 49% at six trees (40 m) from a block of Shepard trees to 30% at thirty trees (160 m) from a block of Shepard trees. Tree yield across this distance declined by 44% as a result of a 69% decline in the number of outcrossed fruit per tree, without a significant decline in the number of selfed fruit per tree. Outcrossed Hass fruit were 12% heavier than selfed Hass fruit, with 3% greater diameter and 5% greater length. The study results demonstrate the importance of interplanting Type B avocado pollinisers closely with Type A Hass trees to increase fruit yield and size. [ABSTRACT FROM AUTHOR]

The practice of intercropping in Rosa roxburghii Tratt orchards holds potential for enhancing fruit yield and financial benefits, yet remains insufficiently explored. To address this, we delved into the effects of intercropping on fruit yield and financial viability of R. roxburghii orchards in Longli County, southern China. Orchards of varying ages (4 years old and 5 years old; 7 years old and 8 years old) were subjected to different treatments: (i) Zea mays and Capsicum annuum intercropping, and clean tillage for younger orchards, and (ii) Lolium perenne, natural grass, and clean tillage for older orchards. Each treatment was assessed for its impact on fruit yield and financial benefits. In younger orchards, intercropping with Z. mays and C. annuum did not significantly elevate fruit yield compared to clean tillage in the 4-year-old orchard; however, C. annuum intercropping significantly improved fruit yield in the 5-year-old orchard. Concurrently, intercropping significantly augmented the total financial benefit by 9234.35–10,486.25 CNY ha−1 (Z. mays) and 14,304.90–16,629.18 CNY ha−1 (C. annuum) compared to clean tillage. In older orchards, L. perenne intercropping significantly elevated fruit yield by 598.84–803.64 kg·ha−1, while natural grass reduced it by 394.61–986.24 kg·ha−1, compared to clean tillage. Additionally, L. perenne intercropping significantly boosted the total financial benefit by 8873.92–9956.56 CNY ha−1, whereas natural grass negatively impacted financial benefits by 78.42–2444.94 CNY ha−1 compared to clean tillage. Collectively, our results illustrate that judicious selection of intercrops, based on orchard age and conditions, can significantly enhance both fruit yield and financial advantages in R. roxburghii orchards. This study furnishes vital insights for orchard management and accentuates the prospective merits of intercropping in fruit production systems. [ABSTRACT FROM AUTHOR]

Simple Summary: Genetic diversity of the different origin genotypes is one of the most important topics to evaluate the desired properties and select high-yield genotypes. Coriander (Coriandrum sativum L.) is an annual plant native to the Mediterranean region, Western Europe, and Asia that belongs to the Apiaceae (Umbelliferae) family. The fruits and essential oils of coriander are used for spice, folk remedies, perfumery, food, tobacco, soft and alcoholic beverages, and pharmaceutical industries in the different parts of the world. The yield and some quality characteristics of coriander genotypes of different origins should be investigated for the breeding program. In this study, both phenotypic, morphological, and yield values showed wide variations. Also, some analyses, such as cluster, heat map, and PCA analyses, revealed important results for the coriander genotypes. In this study, 119 different coriander genotypes (38 different countries), including 114 genotypes and five cultivars, were undertaken to characterize the genotypes based on phenotypic, morphological, yield, and International Union for the Protection of New Varieties of Plants (UPOV) properties, along with some chemical properties. The yield components were between 1.34 and 21.49 g for thousand-grain weight, 0.02–9.58 g/plant for fruit yield, 0.01–50.78 g/plant for biological yield, and 8.48–73.36% for harvest index. Similarly, the results of this study revealed significant variations in essential oil (0.05–1.86%v/w) and fixed oil content (10.22–34.03%v/w). The main components of the essential oil were determined as linalool (3.13–45.70%v/v), p-cymene (0.10–15.77%v/v), ɣ-terpinene (0.04–13.80%v/v), while the fixed oil main acids were determined as petroselinic (24.47–87.70%v/v), palmitic (7.13–23.04%v/v), elaidic (1.55–47.44%v/v), and behenic acids (3.17–12.56%v/v). The cluster, heat map, correlation, and principal coordinate (PCA) analyses were conducted to determine the genetic diversity and relationship among the genotypes based on the examined properties. The cluster and heat map analyses showed differences in the same origin genotypes. Petroselinic acid was the major contributing factor for PCA. As a result of this study, Ames 13900 and Ames 18595 genotypes had high values for fruit yield, fixed oil content, and essential oil content. [ABSTRACT FROM AUTHOR]

The growth of root system directly affects the absorption and utilization of soil water and nitrogen, and understanding the responses of root characteristics to water and nitrogen regimes is thus crucial for optimizing water and nitrogen management. The root characteristics of each soil layer, i.e., root length, root surface area, and root volume, as well as fruit yield and water use efficiency of greenhouse tomato under drip fertigation in response to different irrigation levels and nitrogen rates were explored in northwest China. There were four irrigation levels, i.e., 50% ETC (W1), 75% ETC (W2), 100% ETC (W3), and 125% ETC (W4), where ETC is the crop evapotranspiration, and four nitrogen rates, i.e., 0 kg ha−1 (N1), 150 kg ha−1 (N2), 250 kg ha−1 (N3), and 350 kg ha−1 (N4). The results showed that reasonable irrigation and nitrogen regimes (W3N3) significantly increased fruit yield by 31.64% and root length, root surface area, and root volume by 45.03%, 61.24%, and 148.21% compare to W3N1, respectively. The promoting effect of increasing irrigation level on root characteristics increased with soil depth and had the greatest increases in root volume by 27.07%, 123.43%, and 211.47% for the 0–10, 10–20, and 20–30 cm soil layers, respectively. In addition, reducing irrigation level significantly increased the percentages of roots in the top soil by 29.71%, 26.77%, and 18.53% for root length, root surface area, and root volume, respectively. The reasonable nitrogen rate (N3) significantly increased fruit yield by 41.11%, water use efficiency by 34.42%, and root length, root surface area, and root volume by 40.42%, 41.44%, and 112.76%, respectively. The over-application of nitrogen (N4) reduced root characteristics of all soil layers, fruit yield, and water use efficiency. The promoting effect of increasing nitrogen rate on root length of each soil layer decreased with soil depth, by 71.01%, 48.96%, and 15.71% for 0–10, 10–20, and 20–30 cm soil layers, respectively. Irrigation level was the main factor dominating the root growth of each soil layer. The correlation analysis showed that fruit yield had significantly positive correlations with root characteristics in all soil layers, while water use efficiency had significantly positive correlations with the percentages of root length and root surface area in the 0–10 cm soil layer. In conclusion, rational water and nitrogen regimes achieved better fruit yield by promoting root growth of greenhouse tomato, and the water use efficiency of greenhouse tomato was improved by increasing the root percentage in the topsoil layer to alleviate the adverse effects under water stress conditions. This study reveals how irrigation volume and nitrogen application can enhance tomato yield and water use efficiency by regulating root characteristics and vertical root distribution, providing support for understanding the response of root systems to changes in soil water and nitrogen conditions. [ABSTRACT FROM AUTHOR]

Girdling is an important means of improving the yield and quality of jujube trees, but this measure can easily cause injury, or even death, to jujube trees. A technology for increasing yield and improving quality, based on local thermal damage of jujube bark, is proposed to address a series of issues in current jujube-tree-girdling technology. First, we measured the thermophysical parameters of jujube bark and established a heat-transfer model for jujube bark. Then, in order to investigate the impact of local thermal damage on jujube-tree yield and fruit quality, local heating experiments were conducted on jujube-tree bark, using the heat-transfer model. The experimental results indicated that heating the jujube bark at a certain temperature for an appropriate time can effectively improve the yield and quality of jujube fruit. Compared with traditional girdling techniques, this method has less impact on the health of jujube trees and does not form permanent wounds on them. The research results provide new ideas for exploring sustainable yield-increase methods for fruit trees. [ABSTRACT FROM AUTHOR]

Fennel fruits (Foeniculum vulgare Mill.) represent one of the plant-based natural spices. This study aims to improve the fruit yield and essential oil (EO) quality by reducing the undesirable component, estragole, under different fertilizer treatments. The fertilizers included chemical nitrogen, phosphorus, and potassium (NPK), and also the organic additive, rabbit manure (RM). For bio-fertilization, plants were inoculated with a mixture of N-fixing bacteria, and P- and K-solubilizing bacteria with/without vesicular-arbuscular mycorrhizal fungi. The results showed that fruit and EO yield parameters, total phenolic content (TPC), total flavonoid content (TFC), and DPPH scavenging activity of fruit extracts were enhanced by fertilizer treatments in both growing seasons. NPK at 150% of the recommended dose (NPK150) and RM at 60 m3/fed (RM60) recorded the highest values of plant height, umbel number/plant, 100-fruit weight, fruit yield, chlorophyll, carbohydrates, N and P content, EO content, and yield. TPC and TFC were enhanced by using biological fertilizers. DPPH scavenging activity was higher in organically and biologically fertilized fennel. The GC-MS analyses of EO revealed higher contents of the desirable trans-anethole in the organically and chemically fertilized fennel. However, the highest proportion of estragole, the undesirable compound, was recorded for NPK150 and unfertilized plants. On the contrary, increases in the EO content and yield of fennel fruits were achieved by RM along with a reduction in estragole, enhancement in trans-anethole, and increments in other favorable compounds such as fenchone and limonene. In addition, the inhibition of estragole formation was recorded with bio-fertilizers, which also increased the trans-anethole content. Furthermore, the trans-anethole/estragole ratio was significantly higher with the application of organic and bio-fertilization. Hence, organic and bio-fertilizer resources can produce high-quality fennel fruit and EO. The reduction in the use of chemical fertilizers can help to reduce environmental pollution. [ABSTRACT FROM AUTHOR]

Clean tillage frequently causes the loss of soil nutrients and weakens microbial ecosystem service functions. In order to improve orchard soil nutrient cycling, enhance enzyme activities and microbial community structure in a "Jiro" sweet persimmon orchard, sod culture management was carried out to clarify the relationship among soil nutrient, microbial communities, and fruit yield and quality in persimmon orchard. The results showed that sod culture management increased the content of organic matter, total organic carbon, nitrogen, phosphorus, and potassium in the soil, thus improving soil fertility. Compared with clean tillage orchards, sod culture methods significantly increased soil enzyme activities and microbial biomass carbon (MBC) content. The abundance-based coverage estimator (ACE) and the simplest richness estimators (Chao l) indices of the bacterial community and all diversity and richness indices of the fungal community significantly increased in the sod culture orchard, which indicated that sod culture could increase the richness and diversity of the soil microbial community. The dominant bacterial phyla were Proteobacteria (32.21~41.13%) and Acidobacteria (18.76~23.86%), and the dominant fungal phyla were Mortierellomycota (31.11~83.40%) and Ascomycota (3.45~60.14%). Sod culture drove the composition of the microbial community to increase the beneficial microbiome. Correlation analyses and partial least squares path modeling (PLS-PM) comparative analyses showed that the soil chemical properties (mainly including soil organic matter content, total organic carbon content, total potassium content, and total nitrogen content), soil enzyme activities and soil microorganisms were strongly correlated with fruit yield and quality. Meanwhile, soil nutrient, soil enzyme, and soil microbes had also influenced each other. Our results showed that long-term ryegrass planting could improve soil fertility, enzyme activities, and microbial community compositions. Such changes might lead to a cascading effect on the fruit yield and quality of "Jiro" sweet persimmons. [ABSTRACT FROM AUTHOR]

An outdoor experiment was performed for six months to evaluate the effects of organic fertilizers obtained from marine residual materials on strawberry plants. Three types of organic fertilizers were used, i.e., cod (Gadus morhua) bone powder, common ling (Molva molva) bone powder, and pellets obtained by mixing small cod bone powder and rockweed (Ascophyllum nodosum) residues. A tabletop system for strawberry cultivation was designed, in which two bare-root strawberry plants of cultivar 'Albion' were planted in a peat substrate in each pot. Five treatments were applied, i.e., cod bone powder (F1), common ling bone powder (F2), small cod bone powder and rockweed residue pellets (FA), chemical fertilizer (E), and a control (C). The number of leaves and their nutrient content, fruit yield and quality characteristics of the strawberries grown using the organic fertilizers were similar or better than those corresponding to treatments E and C. Organic fertilizers derived from the residues of fish and macroalgae could be a promising alternative to chemical fertilizers in strawberry production. [ABSTRACT FROM AUTHOR]

In viticulture, choosing the most suitable rootstock for a specific scion cultivar is an efficient and cost-effective way to increase yield and enhance the physicochemical characteristics of the fruit. The objective of this study was to evaluate the agronomic performance of the 'BRS Tainá' grapevine on different rootstocks under the conditions of the Sub-Middle São Francisco Valley. The main experimental factor consisted of eight rootstocks (IAC 313, IAC 572, IAC 766, 101-14 MgT, Paulsen 1103, Ramsey, SO4, and Teleki 5C), arranged in randomized blocks with four replicates. The experiment was conducted over four production cycles, from 2021 to 2023, in a commercial crop area in Petrolina, PE, Brazil. There were significant effects of rootstocks for the yield and number of bunches per plant, as well as berry length and firmness. 'BRS Tainá' achieved the highest yield (22.2 kg per plant) when grafted onto the Paulsen 1103 rootstock, which was superior to the yield on 101-14 MgT, IAC 313, and IAC 572 rootstocks. The highest number of bunches (88) was obtained with 'BRS Tainá' grafted on Paulsen 1103, while the lowest number (63) was obtained on IAC 572; both these rootstocks were not significantly different from the other rootstocks. For all scion–rootstock combinations, the mean values for soluble solid (SS) content, titratable acidity (TA), and the SS/TA ratio were similar to those previously described for 'BRS Tainá', meeting the commercialization standard. The results for the yield and number of bunches per plant indicate the suitability of grafting 'BRS Tainá' on Paulsen 1103 under the semi-arid tropical conditions of the São Francisco Valley. [ABSTRACT FROM AUTHOR]

The cacao fruit is a rich source of polyphenols, including flavonoids and phenolic acids, which possess significant health benefits. The accurate identification and quantification of these bioactive compounds extracted from different parts of the cacao fruit, such as pods, beans, nibs, and cacao shells, require specific treatment conditions and analytical techniques. This review presents a comprehensive comparison of extraction processes and analytical techniques used to identify and quantify polyphenols from various parts of the cacao fruit. Additionally, it highlights the environmental impact of these methods, exploring the challenges and opportunities in selecting and utilizing extraction, analytical, and impact assessment techniques, while considering polyphenols' yield. The review aims to provide a thorough overview of the current knowledge that can guide future decisions for those seeking to obtain polyphenols from different parts of the cacao fruit. [ABSTRACT FROM AUTHOR]

Mango sudden death (MSD) or quick decline (QD) is the most destructive disease found in mango orchards of Pakistan and is characterized by collapse of the vascular system by Ceratocystis fimbriata and Lasiodiplodia theobromae. Cultural practices, chemicals, and biological control are the most valuable tools for the management of MSD, but the role of micronutrient deficiencies has remained an area that is heavily ignored by the farming community. To study the impact of micronutrients, four mango orchards were selected at different locations where different combinations of micronutrients, i.e., Zinc (Zn), Boran (B), and Copper (Cu) in the form of Zinc sulphate (ZnSO4), Borax/Boric acid (H3BO3), and Copper Sulphate (CuSO4), were applied both foliar and in drench along with the recommended doses of Nitrogen: Phosphorous: Potassium (NPK), and Farmyard manure (FYM), respectively. The quantities of micronutrients were determined from the soil and leaves before and after application of the treatments. The impact of micronutrients was measured in terms of reduction in disease severity and increase in fruit yield. The results revealed that the application of all three micronutrients both in soil drench and in foliar form significantly decreased the disease severity at three locations and increased the yield in all four mango orchards. Application of ZnSO4 (0.8%), +H3BO3 (0.8%), +CuSO4 (0.5%) and as soil drench ZnSO4 (400 g) + Borax (200 g) + CuSO4 200 g plant−1 proved to be the best treatments, with an average of 12.88 and 14.03% reduction in disease severity and with an average yield of 128 and 119 kg, respectively. The application of micronutrients would be a promising solution in an integrated disease management program used to tackle MSD. [ABSTRACT FROM AUTHOR]

Tomato production faces challenges such as high input costs, pests, diseases, and climate change. Biostimulants like Nurspray®, based on hydroxycinnamic acid oligomers, offer a promising solution. This study evaluated the impact of different application timings of Nurspray® on tomato yield and fruit quality under open-field conditions. Four treatments were tested: T1 (control), T2 (Nurspray® at BBCH 19–51), T3 (Nurspray® at BBCH 19–51–61), and T4 (Nurspray® at BBCH 19–23–51). The results showed significant improvements in root development, canopy cover, and flowering for T3 and T4, with root systems increasing by up to 103% in T4 compared to the control. Fruit yield and quality were enhanced, with a reduction in blossom-end rot (BER) incidence to 0% in T3 and T4, compared to 5% in the control. Additionally, T4 produced the highest dry matter content in fruits. The optimal application strategy was three doses of Nurspray® at BBCH 19–23–51, enhancing both yield and fruit quality. This study highlights the potential of Nurspray® as an organic biostimulant in sustainable tomato farming. [ABSTRACT FROM AUTHOR]

Water is crucial for agriculture and needs to be used effectively due to climate change and drought in the Mediterranean region. For this reason, to adapt to water deficit scenarios, deficit irrigation applications are increasing in importance. The aim of this research was to determine the effect of varying levels of irrigation on growth parameters and concentration of nutrients in tomato plants grown under greenhouse conditions. The irrigation schedule used in this study was designed to include 100% (control), 90%, 80% and 70% of evaporation from the class-A pan. Water deficit was found to cause a stress effect in tomato plants, which was reflected in changes in the physiological function plants, such as flowering and early ripening. In addition, the SPAD values were examined, for which the lowest value of the green color intensity of the leaves was 47.3 (I3) and the highest was 48.7 (I4). However, the results of statistical analyses show that the difference was not significant. We also observed that the height values of tomato plants were the highest in the period of seedling and fruit ripening under full irrigation. Furthermore, analysis of the macronutrient content of tomato leaves showed that the obtained values were below the threshold values recommended for manganese. Based on these and similar studies, we believe that the application of water stress is most effective during the phases in which the plants are least affected. We believe that determining the periods during which tomato or any other crop is be affected by the least water stress will be more accurate for both plant development and economic production. [ABSTRACT FROM AUTHOR]

Verticillium dahliae (Vd) and Fusarium oxysporum f. sp. lycopersici (Fol) are two major fungal pathogens that infect tomato plants, causing significant challenges in their control since both pathogens can persist in the soil for several years even in the absence of a host plant and no effective fungicides are available at present. This study investigated the efficacy of two biocontrol formulations, Clonotri (containing Trichoderma and Clonostachys microorganisms) and Strepse (comprising Streptomyces and Pseudomonas microorganisms), against Vd and Fol and their impact on tomato fruit quality and yield under greenhouse conditions. The pathogenicity experiment demonstrated that the Clonotri formulation, containing Trichoderma and Clonostachys spores, significantly reduced Fusarium wilt disease by 32% compared to the control group. However, in the Vd pathogenicity experiment, the formulations did not exhibit disease reduction, although treatment with Strepse, containing Streptomyces and Pseudomonas microorganisms, resulted in a preserved total fruit number when compared to uninfected plants. Analysis of fruit quality attributes revealed no significant differences among the various interventions. Furthermore, Fol infection in the first fruit set significantly increased fruit firmness, while Vd infection resulted in elevated levels of total soluble solids in fruits. These findings demonstrate that the evaluated biocontrol formulations provide a degree of protection against fungal wilt pathogens in tomato plants and can increase yield in greenhouse conditions while having minimal impact on overall fruit quality attributes. [ABSTRACT FROM AUTHOR]

The accurate identification of citrus fruits is important for fruit yield estimation in complex citrus orchards. In this study, the YOLOv7-tiny-BVP network is constructed based on the YOLOv7-tiny network, with citrus fruits as the research object. This network introduces a BiFormer bilevel routing attention mechanism, which replaces regular convolution with GSConv, adds the VoVGSCSP module to the neck network, and replaces the simplified efficient layer aggregation network (ELAN) with partial convolution (PConv) in the backbone network. The improved model significantly reduces the number of model parameters and the model inference time, while maintaining the network's high recognition rate for citrus fruits. The results showed that the fruit recognition accuracy of the modified model was 97.9% on the test dataset. Compared with the YOLOv7-tiny, the number of parameters and the size of the improved network were reduced by 38.47% and 4.6 MB, respectively. Moreover, the recognition accuracy, frames per second (FPS), and F1 score improved by 0.9, 2.02, and 1%, respectively. The network model proposed in this paper has an accuracy of 97.9% even after the parameters are reduced by 38.47%, and the model size is only 7.7 MB, which provides a new idea for the development of a lightweight target detection model. [ABSTRACT FROM AUTHOR]

Implementing sustainable crop protection practices is crucial to protect global harvests and ensure high-quality food supplies. While priming is an established method in seed production for the fortification of plants against various stresses, it is not yet a standard practice in transplant cultivation. Thus, we evaluated the long-term effects of thermopriming—a heat-based priming technique—on the growth, development, and fruit yield of tomato plants. Following a recovery period of about six weeks for thermoprimed plants without stress inducers, we subjected them to subsequent salt stress to ascertain the persistence of the priming effects. Additionally, we compared the efficacy of thermopriming with benzothiadiazole (BTH), a chemical elicitor, in enhancing plant resilience to abiotic stress. While BTH application negatively impacted both plant growth and fruit health, thermopriming showed no such adverse effects on these parameters. Instead, thermopriming initially enhanced the plant defense mechanisms by increasing the accumulation of protective phenols and flavonoids in the leaves. Interestingly, while thermopriming did not alter the response to salt stress, it notably strengthened the overall resilience of the plants. Our findings underscore both the potential and temporal constraints of thermopriming memory. Nonetheless, primed plants exhibited temporarily increased stress tolerance, offering a means to safeguard the offspring. [ABSTRACT FROM AUTHOR]

Drought stress is one of the main abiotic stresses that limit plant growth and affect fruit quality and yield. Plants primarily lose water through leaf transpiration, and wax effectively reduces the rate of water loss from the leaves. However, the relationship between water loss and the wax formation mechanism in goji (Lycium barbarum) leaves remains unclear. 'Ningqi I' goji and 'Huangguo' goji are two common varieties. In this study, 'Ningqi I' goji and 'Huangguo' goji were used as samples of leaf material to detect the differences in the water loss rate, chlorophyll leaching rate, wax phenotype, wax content, and components of the two materials. The differences in wax-synthesis-related pathways were analyzed using the transcriptome and metabolome methods, and the correlation among the wax components, wax synthesis genes, and transcription factors was analyzed. The results show that the leaf permeability of 'Ningqi I' goji was significantly lower than that of 'Huangguo' goji. The total wax content of the 'Ningqi I' goji leaves was 2.32 times that of the 'Huangguo' goji leaves, and the epidermal wax membrane was dense. The main components of the wax of 'Ningqi I' goji were alkanes, alcohols, esters, and fatty acids, the amounts of which were 191.65%, 153.01%, 6.09%, and 9.56% higher than those of 'Huangguo' goji, respectively. In the transcriptome analysis, twenty-two differentially expressed genes (DEGs) and six transcription factors (TFs) were screened for wax synthesis; during the metabolomics analysis, 11 differential metabolites were screened, which were dominated by lipids, some of which, like D-Glucaro-1, 4-Lactone, phosphatidic acid (PA), and phosphatidylcholine (PE), serve as prerequisites for wax synthesis, and were significantly positively correlated with wax components such as alkanes by the correlation analysis. A combined omics analysis showed that DEGs such as LbaWSD1, LbaKCS1, and LbaFAR2, and transcription factors such as LbaMYB306, LbaMYB60, and LbaMYBS3 were strongly correlated with wax components such as alkanes and alcohols. The high expression of DEGs and transcription factors is an important reason for the high wax content in the leaf epidermis of 'Ningqi I' goji plants. Therefore, by regulating the expression of wax-synthesis-related genes, the accumulation of leaf epidermal wax can be promoted, and the epidermal permeability of goji leaves can be weakened, thereby reducing the water loss rate of goji leaves. The research results can lay a foundation for cultivating drought-tolerant goji varieties. [ABSTRACT FROM AUTHOR]

An open-field experiment was conducted in Cabuyao, Laguna, Philippines to investigate the effects of combining chemical fertilizers, vermicompost, arbuscular mycorrhizal fungi (AMF), and nitrogen-fixing bacteria (Azospirillum spp.) on the growth, nutrient uptake, and yield of tomato plants. The experiment was arranged in a randomized complete block design replicated four times. The treatments include the recommended rate of chemical fertilizer (RRC) and three integrated nutrient management (INM) strategies. The results revealed that AMF inoculation significantly increased the uptake of P, K, Ca, and Mg while INM 3 recorded the highest N uptake. Treatments with vermicompost application recorded a significantly higher uptake of Cu. INM 1, INM 2, and INM 3 gave a significantly higher fruit yield than the RRC with an increase of 8%, 13%, and 14%, respectively. The percentage of mycorrhizal root colonization and number of rhizosphere spores were higher in mycorrhizal plants. Fruit yield and AMF root colonization were positively correlated with the uptake of several nutrients. INM strategies obtained a higher net income than the current fertilizer recommendation by 4–15%. These findings imply that the INM strategies can increase tomato productivity, reduce the amount of chemical fertilizer inputs, increase profitability, and potentially lead to soil health and environmental benefits. [ABSTRACT FROM AUTHOR]

Light downconversion films can modulate incident light wavebands on crops, converting less utilised wavebands in an efficient way. In this experiment, red (conversion of green into red light wavebands), pink (conversion of UV and green into blue and red light but to a smaller degree than red film), and blue (conversion of UV into blue light) light downconversion films were used to cover blackberry plants throughout all phenological stages (from leaf emergence to fruit harvesting). The plants' physiological and biometric performance, and fruit yield and quality were evaluated. Plants under blue and red films showed a higher net photosynthetic rate with +23.1% and +14.9%, respectively, and a higher stomatal conductance with +56.0% and +23.6%, respectively, with respect to controls, maintaining stability across stages, except for a decrease under the red film during fruiting. Both films significantly boosted the fruit yield, with the red film increasing the fruit number (+49.8%) and the blue film enhancing the berry shape (+10.7) and fresh weight (+36.6). Notably, no significant differences in nutraceutical quality, including total flavonoid and anthocyanin content, were observed. These findings suggest that light downconversion films, particularly red and blue films, can effectively enhance the photosynthetic performance and fruit production in blackberry plants without compromising the fruit quality. Future research on this topic should focus on balancing plant growth, fruit productivity, and enhancing fruit nutraceutical properties. [ABSTRACT FROM AUTHOR]

Cucumber is a vegetable crop of economic importance in Mexico. In 2023, fresh or frozen cucumber and pickle international sales reached USD 783M, placing Mexico in second rank among countries with the highest currency inflow in the world. This crop is mostly grown under protected environments with new production technologies, including rootstocks that are tolerant to biotic and abiotic factors that limit the yield and quality of the harvest. Therefore, the goal of this work was to assess three cucurbits as rootstocks (Cucurbita maxima, Cucurbita moschata and Citrullus lanatus), as well as studying the response of cucumber crop to the tested rootstocks. The following treatments were assessed: T1:PSP = cucumber Cv. Paraizo F1 without rootstock; T2:P/MA = cucumber grafted over Cucurbita maxima; T3:P/MO = cucumber grafted over Cucurbita moschata; T4:P/CL = cucumber grafted over Citrullus lanatus Citroides Var. The assessed variables included the number of fruits per plant (NFPP), fruit weight per plant (PFPP), average fruit weight (PPF), plant dry weight (PSP), xylem vessel area (AVX), number of xylem vessels (NVX), vascular bundle area (AHV) and number of vascular bundles (NHV) in roots (R), basal stalk (TB) and apical stalk (AT). Significant differences among treatments were found in the results for variables: NFPP, highlighting that T2:P/MA was superior by 46.15% versus T1:PSP and by 67.69% versus T3:P/MO. Regarding PFPP, T2:P/MA achieved the highest weight (5445 g), as it proved superior by 99.45% than the cucumber without rootstock (T1:PSP). Regarding PSP, T2:P/MA achieved the highest weight (196.65 g), significantly exceeding T1 by 137.5%. Regarding AVXR, T4:P/CL had the highest value (30,063 µm2). Regarding NHVR, watermelon had (T4:P/CL) 39.33% more vascular bundles than the root system of cucumber (T1:PSP). Treatments T2:P/MA and T4:P/CL had the highest values at AVXTB, while for variable NVXTB, treatments T3:P/MO and T2:P/MA were almost equal, surpassing non-grafted cucumber by 175.47%. However, T2:P/MA showed the greatest AHVTB, and T1:PSP was significantly superior to T3:P/MO, by 34.93%, for NHVTB. Furthermore, positive and significant correlations were found among PFPP, AHVR, and AVXTB, as well as among PSP, AVXTB, and AHVTB. Therefore, we may say that having a larger area of vascular vessels in the roots and a larger area of xylem vessels in the basal stalk leads to higher fruit yields per plant. In the same vein, the use of rootstocks is a sustainable alternative to increase fruit yield. [ABSTRACT FROM AUTHOR]

Pomegranate (Punica granatum L.) is a fruit tree that is globally distributed, especially in warm areas with low annual rainfall and limited water availability. This species exemplifies the critical role of water in agriculture and the need for efficient irrigation practices due to its characteristics, cultivation requirements, and geographic diffusion. In this study, we investigated the effects of drip irrigation and mulching on the vegetative growth, yield, and fruit quality attributes of pomegranate. The experiment involved three irrigation regimes (100% of evapotranspiration, 80%, and 60%) and three mulching treatments (no mulch, plastic mulch, and organic mulch) in a factorial combination. Both irrigation and mulching had significant positive influences on the yield and fruit quality attributes. Specifically, deficit irrigation strategies showed a negative impact on the fruit yield per tree, with a greater effect observed as the severity of the irrigation deficit increased. Mulching, on the other hand, led to a significant increase in the fruit yield, primarily attributed to an increase in fruit size. Furthermore, the analysis indicated that irrigation and mulching treatments had distinct effects on fruit traits such as the fruit length, width, volume, and rind thickness. Interestingly, the study highlighted that the effects of irrigation and mulching on fruit quality attributes were mostly independent of each other, suggesting an additive influence rather than an interaction between the two factors. These findings underscore the importance of considering irrigation and mulching practices for optimizing fruit quality in pomegranate cultivation, particularly in semi-arid regions. The results contribute valuable insights for farmers and researchers seeking to enhance fruit production and quality. [ABSTRACT FROM AUTHOR]

To overcome the scarcity of fresh water, researchers have turned to investigating different techniques that enable using saline water to irrigate crops, aiming to increase the efficiency of using available water resources. A glasshouse experiment was conducted to investigate the yield responses of grafted and non-grafted (self-rooted) tomato plants grown hydroponically under three levels of water salinity (2.5, 6.0, and 9.5 dS m−1). Three tomato varieties (Ghandowra-F1, Forester-F1, and Feisty-Red) were grafted onto five rootstocks (Maxifort, Unifort, Dynafort, Vivifort, and Beaufort). The implemented treatments were studied in terms of tomato fruit yield and quality parameters. Although increasing the concentration of salts in the nutrient solution led to a decrease in fruit yield, the moderate salinity level (S-2: 6.0 dS m−1) showed its superiority over both low salinity (S-1: 2.5 dS m−1) and high salinity (S-3: 9.5 dS m−1) in terms of tomato yield parameters. The studied rootstocks did not significantly improve the tomato fruit yield, but the interaction between the grafting combinations and salinity was significant for both production and quality. More specifically, tomato plants grafted onto the rootstocks "Vivifort and Beaufort" rendered the highest yield at a low salinity level (S-1: 2.5 dS m−1) and a moderate salinity level (S-2: 6.0 dS m−1), respectively, while at high salinity (S-3: 9.5 dS m−1), grafting did not improve tomato productivity, irrespective of the rootstock. These results confirm that tomatoes can be successfully grown under hydroponic systems using salinity levels of up to 6.0 dS m−1 without sacrificing fruit yield and quality. Among the studied tomato varieties, Feisty-Red was found to be appropriate for hydroponic production. The results also demonstrated that Vivifort and Beaufort rootstocks are suitable for grafting hydroponic tomatoes under low and moderate salinity levels, respectively. [ABSTRACT FROM AUTHOR]

Intercropping orchards with cover crops is an important practice for achieving sustainable soil management. However, little research has addressed the development of a soil quality index (SQI) to evaluate cover crop effects on orchard soil quality. The aim of this study was to ascertain whether cover cropping improves soil quality and fruit yield of Goji (Lycium barbarum L.) while reducing or replacing organic fertilizer application. The main treatments were the traditional management of L. barbarum as a monocrop (M) and intercropping Goji with radish (Raphanus sativus L.) as an annual cover crop (I). Within the main treatments, different levels of organic fertilizer were applied at 0 kg·plant−1 (M0), 2 kg·plant−1 (M1), and 4 kg·plant−1 (M2). After six years of planting, we analyzed the changes in soil quality caused by cover cropping with different organic fertilizer levels based on the SQI method. Goji yields were used for validation of the SQI derived from a minimum data set of soil quality indicators. In contrast with traditional monocropping, cover cropping increased soil total nitrogen, available nitrogen, and available phosphorus contents (by 78.60%, 30.30%, and 138.08%, respectively). There were also increased microbial biomass carbon and nitrogen contents (by 79.01% and 184.01%, respectively), enhanced urease and sucrase activities (by 41.02% and 56.81%, respectively), and reduced bulk density (by 1.92%) in the soil as a result of cover cropping. Compared with IM0 treatment, soil microbial biomass carbon and nitrogen contents considerably increased under IM1 treatment, whereas soil available nitrogen and potassium contents as well as electrical conductivity increased under IM2 treatment. The SQI, which varied among treatments in the order IM1 > IM2 > MM2 > MM1 > IM0 > MM0, was positively correlated with Goji yield. From the soil quality and Goji yield perspective, cover cropping with a medium level of organic fertilizer is the optimal soil management practice for the L. barbarum planting system in arid areas of Ningxia, Northwest China. [ABSTRACT FROM AUTHOR]

The size and quality of the melon fruit yield depend on the cultivar, climatic and agronomic factors. A three-year field experiment investigated the effect of arbuscular mycorrhizal fungi (AMF) application and irrigation (IR) on the fruit yield of melon (Cucumis melo L. var. saccharinus Naud.) cultivars. The study was conducted on a certified organic farm located in south-eastern Poland (51.36° N, 22.83° E). The factors of the experiment were as follows: cultivar (Melba, Emir F1, Seledyn F1, Oliwin) and cultivation method (AMF and IR; AMF and non-IR; non-AMF and IR; non-AMF and non-IR as control). The dry matter (%), soluble solid (%), total sugar and reducing sugar (% fresh weight, FM), L-ascorbic acid (mg · 100 g−1 FM), and carotenoid (mg · 100 g−1 FM) contents of the fruit were determined. The highest total and marketable fruit yields were obtained using AMF and IR. Fruit from the AMF series (IR and non-IR) had the most carotenoids (respectively: 801.5 and 788.8 µg · 100 g−1 FM). The fruits of the AMF and IR plants contained the most total sugars (5.98%) and reducing sugars (2.91%) compared to the others. The control plants had the lowest number of marketable fruit, total and marketable fruit yield, and accumulated the least L-ascorbic acid, total sugars and reducing sugars. We suggest that AMF and IR can be recommended as a practical agronomic solutions for the field cultivation of melon under temperate climate conditions. [ABSTRACT FROM AUTHOR]

Organic materials added to soil create anaerobic conditions that can reduce soil-borne pathogens that reduce the yield and quality of agricultural crops. Anaerobic soil disinfestation (ASD) requires relatively large quantities of readily available, inexpensive organic materials. We evaluated the impact of ASD with rice bran and biochar organic materials on changes to the soil's physicochemical properties, microbial taxa, and strawberry fruit yield. We found that the organic materials applied at different dose rates significantly increased the control effect of the soil Fusarium spp. and Phytophthora spp. to 69–99% and 63–98%, respectively. In addition, ASD significantly increased soil organic matter and ammonium nitrogen contents. Strawberry yield also increased significantly after ASD treatment with biochar applied at 10 t/ha, which was positively correlated with increased soil nutrients and a significant reduction in pathogens. High-throughput gene sequencing showed that ASD significantly increased the abundance of some beneficial microorganisms such as Bacillus, Pseudomonas, and Mortierella, possibly due to changes in the soil's physicochemical properties that favored their survival. We found for the first time that biochar applied at 10 t/ha could create anaerobic conditions that effectively reduced soil-borne pathogens and increased crop yield. [ABSTRACT FROM AUTHOR]

We studied the effect of shade on the phenology, growth, berry yield, and chemical content of two common blueberry species (Vaccinium myrtilloides and V. angustifolium) in Northwestern Ontario. We hypothesized that high shade would delay vegetative and reproductive phenology and decrease berry yield by increasing resource allocation to vegetative vs. reproductive growth, whereas moderate shade would increase berry phenolic content and antioxidant capacity. We subjected transplanted blueberry plants to a controlled shade treatment and evaluated plant phenological events, vegetative and reproductive growth, berry phenolics, and antioxidant capacity. High shade caused an earlier leaf maturation in V. myrtilloides, delayed flowering in V. angustifolium, and prolonged fruit maturation in both. The berry yield of both species decreased with increasing shade. High shade reduced the berry phenolic content and antioxidant capacity, especially in V. myrtilloides. We concluded that shade shifts species-specific vegetative and reproductive phenology, leading to a difference in resource acquisition, resulting in lower berry yield and antioxidant activity. [ABSTRACT FROM AUTHOR]

The use of covers to protect blueberry orchards from adverse weather events has increased due to the variability in climate patterns, but the effects of rain covers and netting materials on yield and fruit quality have not been studied yet. This research evaluated the simultaneous effect of an LDPE plastic cover, a woven cover, and netting material on environmental components (UV light, PAR, NIR, and growing degree days (GDDs)), plant performance (light interception, leaf area index, LAI, yield, and flower development), and fruit quality traits (firmness, total soluble solids, and acidity) in two blueberry cultivars. On average, UV transmission under the netting was 11% and 43% higher compared to that under woven and LDPE plastic covers, while NIR transmission was 8–13% higher with both types of rain covers, with an increase in fruit air temperature and GDDs. Yield was 27% higher under the woven cover with respect to netting, but fruit firmness values under the netting were 12% higher than those of the LDPE plastic cover. Light interception, LAI, and flower development explained 64% (p = 0.0052) of the yield variation due to the cover material's effect. The obtained results suggest that the type of cover differentially affects yield and fruit quality in blueberries due to the specific light and temperature conditions generated under these materials. [ABSTRACT FROM AUTHOR]

The importance of implementing recirculating drainage for greenhouse strawberries is often overlooked because of the low electrical conductivity (EC) of drainage and transpiration despite the large area of cultivation in Korea. In this study, we analyzed the growth of strawberry crops and their water and individual nutrient use efficiency when using closed-loop hydroponics in greenhouses. The study consisted of two parts: Experiment (Exp) 1 and Exp 2, each of which employed a different closed-loop hydroponic control method. In Exp 1, the system was controlled solely based on the EC of the drainage mixed with raw water. In Exp 2, the nutrient solution (NS) was corrected according to the ion concentrations in the drainage, with correction intervals of 2 weeks, 4 weeks, and no correction, while the control treatment was open-loop. In Exp 1, a prolonged increase in drainage EC resulted in an imbalance in NS ion composition and reduced fruit yield. In Exp 2, the NS composition was stabilized through periodic nutrient correction, and the fruit yield of the closed-loop treatments did not differ from the open-loop treatment. However, the closed-loop with 2- and 4-week correction in Exp 2 showed 94% and 88% higher nutrient use efficiency (NUE), respectively, than an open-loop system. Among the closed-loop treatments, the 2- and 4-week correction intervals had 36% and 32% higher NUE than the no-correction treatment. Furthermore, the 2-week correction interval showed a 3% improvement in NUE compared to the 4-week interval. These findings highlight the importance of considering a shorter ion correction interval for optimal NUE and normal plant growth in recirculating hydroponic systems of strawberry cultivation. [ABSTRACT FROM AUTHOR]

This study evaluated the effect of salicylic acid (SA) application on yield, fruit quality, and post-harvest storage in Prunus avium subjected to deficit irrigation (DI). A field experiment with six-year-old P. avium cv. Lapins was performed under two water treatments: irrigation at 100% of crop evapotranspiration (ETc) [full irrigation (FI)] and irrigation at 60% ETc from the second fruit phase to harvest time (DI). A single 0.5 mM SA was applied to both water treatments at fruit color change. At harvest time, fruits were collected to determine yield, fruit quality, and quality during post-harvest storage (0, 10, 20, and 30 days). The DI reduced fruit yield (11%), fruit weight (8%), and caliber (6%) and increased firmness (7%) and total soluble solids (TSS) (5%) in P. avium compared with FI plants at harvest time. Our study showed that SA application recovered fruit yield (9%), fruit weight (5%), and caliber (4%), improving TSS in DI plants at day 0. Interestingly, SA application significantly reduced P. avium fruit cracking (78% in FI and 82% in DI). Fruit weight was reduced in all treatments, mainly decreasing by 14% in FI and 13% in DI plants at day 30 of post-harvest storage. Fruit weight did not change during post-harvest storage with SA, except on day 30, where a slight reduction was observed. TSS showed no significant differences during post-harvest storage for all treatments. Therefore, SA could be an interesting tool to mitigate the impact of DI on the yield and fruit quality of P. avium and to reduce fruit cracking and prolong fruit quality during post-harvest storage. [ABSTRACT FROM AUTHOR]

Nitrogen (N) fertilizer is routinely applied in highbush blueberry (Vaccinium corymbosum L.) production. The recommended N fertilizer rate increases as the plants mature, and is usually determined based on regional growing conditions. However, the effects of N fertilizer rates and application methods over the long term remain poorly understood. In this study, ammonium sulfate was applied as an N source at the recommended rate (100%), which corresponds to a maximum of 155 kg N ha−1 for plants older than eight years, along with higher rates at 150% and 200% of the recommended level, as well as a control treatment of no N. Treatments were applied to the blueberry cultivar 'Duke' as either broadcast (BROAD) or fertigation (FERT), and impacts were analyzed after 12 and 13 years of treatment. In the 14th year, the 100% N rate was uniformly applied as BROAD across all plants to separate the effects of different N rates from those caused by long-term soil condition changes. The BROAD treatment at the 100% N rate achieved the highest yield, and the FERT treatment at 200% resulted in the lowest yield in the 12th year, suggesting that excessive N rates can reduce fruit yield. However, no significant yield differences were observed in the 13th year. Higher N rates were associated with reduced titratable acidity in fruits and fewer flower buds. The soil pH declined across all N treatments, with the FERT at 200% showing the most significant reduction. All N treatments generally increased soil electrical conductivity (EC). High N rates also decreased plant accumulation of magnesium, calcium, and copper, with the latter reaching deficiency levels. These findings emphasize the importance of adhering to recommended N application rates and adjusting soil pH and EC to mitigate the adverse effects of prolonged N treatments. [ABSTRACT FROM AUTHOR]

Tomato (Solanum lycopersicum L.), the second most important vegetable crop globally, faces a significant threat from various viral diseases. A newly emerging disease, characterised by distinctive shoestring symptoms on leaves and the development of unripe, small, and hard fruit, poses a serious challenge to tomato cultivation in India. An initial survey in an experimental field revealed more than 50% of the plants displayed symptoms of the shoestring disease, resulting in substantial reductions in fruit yield and quality. Transmission electron microscopy (TEM) and molecular analyses identified an isolate of the tomato mottle mosaic virus (ToMMV) in the affected plants. When the partially purified virus was mechanically inoculated into tomato cv. Pusa Ruby plants, it reproduced the characteristic shoestring symptoms, confirming its causal relationship with the disease. Notably, the present shoestring isolate of ToMMV (ToMMV-Tss) was found to induce similar shoestring symptoms in most of the major commercial tomato varieties when inoculated under controlled experimental conditions in the glasshouse, indicating its aggressive nature. Host range studies demonstrated that the ToMMV-Tss can infect several solanaceous species, while cucurbitaceous hosts remained unaffected. Moreover, the virus was found to be seed-transmissible, with a small percentage of seedlings from infected plants displaying symptoms. These findings underscore the significant impact of ToMMV on tomato production in India and emphasise the need for reliable diagnostic tools and effective management strategies to curb the spread and mitigate the impact of this virus on commercial tomato cultivation. [ABSTRACT FROM AUTHOR]

Climate change has emerged as a crucial global issue that significantly threatens the survival of plants. In particular, low temperature (LT) is one of the critical environmental factors that influence plant morphological, physiological, and biochemical changes during both the vegetative and reproductive growth stages. LT, including abrupt drops in temperature, as well as winter conditions, can cause detrimental effects on the growth and development of tomato plants, ranging from sowing, transplanting, truss appearance, flowering, fertilization, flowering, fruit ripening, and yields. Therefore, it is imperative to understand the comprehensive mechanisms underlying the adaptation and acclimation of tomato plants to LT, from the morphological changes to the molecular levels. In this review, we discuss the previous and current knowledge of morphological, physiological, and biochemical changes, which contain vegetative and reproductive parameters involving the leaf length (LL), plant height (PH) stem diameter (SD), fruit set (FS), fruit ripening (FS), and fruit yield (FY), as well as photosynthetic parameters, cell membrane stability, osmolytes, and ROS homeostasis via antioxidants scavenging systems during LT stress in tomato plants. Moreover, we highlight recent advances in the understanding of molecular mechanisms, including LT perception, signaling transduction, gene regulation, and fruit ripening and epigenetic regulation. The comprehensive understanding of LT response provides a solid basis to develop the LT-resistant varieties for sustainable tomato production under the ever-changing temperature fluctuations. [ABSTRACT FROM AUTHOR]

Seasonal drought and summer soil high-temperature stress in Southern China often lead to decreased yield and quality of Annona squamosa. It is important to explore reasonable and effective water and fertilizer management measures as well as cover measures to improve the soil hydrothermal conditions in orchards to realize the increase in yield and quality of Annona squamosa. This study involved a two-year (2022–2023) field experiment in Yun County, Lincang City, Yunnan Province, using three factors and a three-level orthogonal test, resulting in nine different experimental treatments for water, fertilizer and heat. The three irrigation levels were W1 (soil moisture content of 55% of field moisture capacity), W2 (soil moisture content of 75% of field moisture capacity) and W3 (soil moisture content of 85% of field moisture capacity). The three fertilizer levels were F1 (1666 kg·hm−2), F2 (2083 kg·hm−2) and F3 (2500 kg·hm−2), and the three cover methods were A1 (no cover), A2 (fresh grass cover) and A3 (straw cover). The effects of these treatments on soil hydrothermal conditions, growth indices and fruit yield and quality of Annona s1uamosa were systematically monitored and analyzed, and the relationships between these treatments and yield and quality was analyzed based on a Mantel test. The results showed that T5 (W2F2A3) had the highest average soil moisture content over two years, followed by T7 (W3F1A3). The T7 (W3F1A3) treatment effectively reduced soil temperature by 5 °C compared to T1 (W1F1A1). T5 (W2F2A3) had the highest average yield over two years, with an increase of 33.99% compared to T1 (W1F1A1). Additionally, T5 (W2F2A3) has the highest average soluble solids, soluble sugars and vitamin C content over two years, with increases of 28.13%, 13.36% and 4.86%, respectively, compared to T1 (W1F1A1). A Pearson correlation analysis showed that there was a significant correlation between Annona squamosa growth and soil moisture content and soil temperature, and the Mantel test showed that soil hydrothermal conditions had significant influence on the growth and yield. T5 (W2F2A3) has the highest comprehensive benefit in promoting growth, increasing yield and improving quality for the plant. The effects of different irrigation quantities, fertilizer amounts and different cover measures on the coupling interaction for soil hydrothermal status in the root zone, growth, yield and quality of Annona squamosa were investigated, providing reliable theoretical support for the scientific planting model of Annona squamosa in the low-heat river valley of Yunnan Province. [ABSTRACT FROM AUTHOR]

At a time when growers have to increase food production, while facing many environmental challenges, biostimulants and plant defence stimulators (PDS) may help reduce the use of chemical fertilizers and pesticides and to promote agriculture that is more respectful of the environment. For organic farming, they may contribute to increasing plant resilience and crop productivity. Several studies have shown that plant-derived protein hydrolysates may increase nutrient use efficiency and promote plant resistance to abiotic or biotic stresses. We therefore hypothesized that soy protein hydrolysates increase tomato growth and productivity, while promoting plant disease resistance. Our results showed that one or two drench applications of soy protein hydrolysates (SPH13 and SPH18 at 10 g L−1) to the growing medium increased tomato ('Micro Tom') plant growth and fruit production, while one studied hydrolysate enriched in glycine (SPH18) increased the expression levels of PR1 and PR8, two defence-related genes. Although no significant effect was observed on Botrytis cinerea resistance of Micro Tom plants, SPHs significantly increased 'M82' plant resistance to foliar inoculation with Pseudomonas syringae pv. tomato DC3000, which further confirmed the systemic activation of plant defence mechanisms by SPHs in tomatoes. [ABSTRACT FROM AUTHOR]

Carbon dioxide enrichment inside a greenhouse is a sustainable approach to increasing crop production worldwide. Recently, the F1 hybrid strawberry became an alternative to runner-propagated cultivation as an innovative method to shorten the production period and increase strawberry production. This work aims to present CO2 enrichment as a sustainable tool that improves the yield in a controlled greenhouse and addresses the efficiency of three F1 hybrid strawberry varieties grown under Saudi Arabian conditions. A greenhouse experiment was conducted at the National Research and Development Center for Sustainable Agriculture (Estidamah), KSA, to study the impact of two CO2 levels (400 ppm ("ambient") and 600 ppm ("enrichment")) on the growth, photosynthesis traits, fruit yield and fruit quality of three F1 hybrid strawberry varieties grown under soilless culture conditions. The results show that CO2 enrichment significantly improved the phenotyping of strawberry growth traits at 60 days post-transplanting. The physiological response of the varieties to CO2 enrichment reveals a significant increase in the photosynthetic rate (129.7%) and intercellular CO2 (43.7%) in the leaves of strawberry exposed to CO2 enrichment rather than in ambient conditions, combined with a significant increase in the number of fruits per plant (27.5%) and total fruit yield (42.2%). A similar pattern was observed with varieties D and S in terms of fruit number, length and diameter. However, CO2 at 600 ppm promoted total soluble solid accumulation and vitamin C for the tested varieties. In contrast, CO2 enrichment significantly decreased nitrogen, phosphorus, potassium and magnesium accumulation in the leaves of the exposed plants in comparison to 400 ppm of CO2. These results suggest that increasing CO2 enrichment could contribute to an increase in strawberry yield and nutritional value and demonstrate that understanding the response of each variety to CO2 enrichment is important to support selecting suitable greenhouse strawberry varieties to improve crop yield. [ABSTRACT FROM AUTHOR]

The global agriculture industry is encountering challenges due to labor shortages and the demand for increased efficiency. Currently, fruit yield estimation in guava orchards primarily depends on manual counting. Machine vision is an essential technology for enabling automatic yield estimation in guava production. To address the detection of guava in complex natural environments, this paper proposes an improved lightweight and efficient detection model, V-YOLO (VanillaNet-YOLO). By utilizing the more lightweight and efficient VanillaNet as the backbone network and modifying the head part of the model, we enhance detection accuracy, reduce the number of model parameters, and improve detection speed. Experimental results demonstrate that V-YOLO and YOLOv10n achieve the same mean average precision (mAP) of 95.0%, but V-YOLO uses only 43.2% of the parameters required by YOLOv10n, performs calculations at 41.4% of the computational cost, and exhibits a detection speed that is 2.67 times that of YOLOv10n. These findings indicate that V-YOLO can be employed for rapid detection and counting of guava, providing an effective method for visually estimating fruit yield in guava orchards. [ABSTRACT FROM AUTHOR]

In Florida, almost all citrus trees are affected with Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (CLas). We characterized various parameters of HLB-affected sweet orange trees in response to yield-improving nutritional treatment, including canopy volume, canopy density and CLas Ct values, and found that the treatment improved yield and maintained canopy density for over three years, whereas untreated HLB-affected trees declined in canopy density. The nutritional treatment did not affect CLas titer or the tree canopy volume suggesting that canopy density is a better indicator of fruit yield. To further validate the importance of canopy density, we evaluated three independent orchards (different in tree age or variety) to identify the specific traits that are correlated with fruit yields. We found that canopy density and fruit detachment force (FDF), were positively correlated with fruit yields in independent trials. Canopy density accurately distinguished between mild and severe trees in three field trials. High and low producing HLB trees had the same Ct values. Ct values did not always agree with CLas number in the phloem, as visualized by transmission electron microscopy. Our work identifies canopy density as an efficient trait to predict yields of HLB-affected trees and suggests canopy health is more relevant for yields than the CLas population. [ABSTRACT FROM AUTHOR]

This research examines the effects of two irrigation strategies on water use efficiency and fruit yield components of 'Royal Gala' and 'Brookfield Gala' apple orchards in south-central Chile. The study was carried out during the 2008–2009 and 2009–2010 growing seasons at two sites. A randomized block experimental design was established with two water application treatments: theoretical volume required by the plant (T1) and farm protocol (T2). Soil water content, plant water potential and yield components were evaluated. The soil water content in T2 was near field capacity while T1 was between the permanent wilting point and field capacity for both seasons and varieties. With T1, the seasonal volume applied was 21 to 28% less compared to T2, with season savings of 1600 m3 ha−1. No effect on plant water potential was observed. In 'Royal Gala' the lower volume applied in T1 did not lead to significant differences in fruit diameter, weight, or yield in either season as compared to T2. In 'Brookfield Gala', yield during the 2008–2009 season increased significantly, by 22.9% in treatment T1, and in the 2009–2010 season, significant reductions (p < 0.05) in fruit diameter (5.3%) and weight (12.9%) were observed in T2. Productivity per volume of applied water in T1 was 32% to 56% greater than that obtained with T2. The results show the effectiveness of the irrigation strategy considering the theoretical volume of water required by the plant. [ABSTRACT FROM AUTHOR]

Pre-harvest nitrogen (N) application has been proven effective for improving fruit yield and quality. However, a full understanding of how differences in N availability/plant N status influence the transcriptome and metabolism underlying yield formation and quality remains elusive. Here, a combined analysis of the morpho-physiological qualities, transcriptome, and metabolite of peach plants was performed under different nitrogen levels at fruit pit hardening (PH) and fruit expansion (FE). Nitrogen fertilizer directly affected the yield, fruit quality, and metabolites of peach at different growth stages. RNA-Seq was used to analyze the influence of N levels at PH and FE in peach. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the differentially expressed genes (DEGs) focused on flavonoid biosynthesis and secondary metabolite biosynthetic processes. The differential metabolites among the different treatments were mainly involved in flavonoid metabolism. Transcriptome analysis showed that applying different nitrogen fertilizers at different growth stages of peach mainly affected the synthesis of flavonoids in fruit. Overall, these results suggest that the impacts of pre-harvest N application on fruit yield and quality differ between developmental stages. This research provides a full understanding of the metabolic processes underlying fruit growth and development in peach, providing a theoretical basis for the improvement of nitrogen use efficiency in peach trees. [ABSTRACT FROM AUTHOR]

The citrus yellow vein clearing virus (CYVCV) is an emerging threat to the U.S. citrus industry. Reports from China shows it cause significant reductions in fruit yield and growth, particularly in lemon trees. In 2022, CYVCV was detected in a wide range of citrus cultivars in localized urban properties in Tulare, California. In 2024, a CYVCV-infected lemon tree was detected in Hacienda Heights in Los Angeles County, California, geographically separated from the Tulare foci. Through long-read sequencing technology, the whole-genome sequence of a CYVCV isolate from Hacienda Heights (designated as CYVCV-CA-HH1, Accession number PP840891.1) was obtained. Sequence alignments and neighbornet analysis strongly suggested that the CYVCV-CA-HH1 isolate has a different origin than the Tulare CYVCV (CYVCV CA-TL) isolates. The CYVCV CA-TL isolates were grouped with those from South Asia (India and Pakistan) and the Middle East (Türkiye), while the CYVCV-CA-HH1 isolate was grouped with isolates from East Asia (China and South Korea). Maximum likelihood phylogenetic analysis further supports this finding, showing that the CYVCV-CA-HH1 isolate shares the most recent common ancestor with a South Korean lineage, which derives from Chinese isolates. Together, our data suggest a diverse geological origin of CYVCV isolates in California. [ABSTRACT FROM AUTHOR]

Olive anthracnose outbreaks caused by the Colletotrichum species complex in the Mediterranean region decrease both fruit yield and olive oil production while also drastically degrading olive oil quality. The presence of various Colletotrichum species able to produce disease symptoms in olive fruits significantly deteriorates the efforts for an efficient crop protection strategy. In this report, the major olive productive area of Peloponnese was screened for Colletotrichum species capable of generating anthracnose symptoms. Olive fruits of 12 different olive cultivars were collected from 60 groves distributed analogously in the Peloponnese. Thirty-two fungal strains isolated from asymptomatic olive drupes were identified morphologically as Colletotrichum spp. and were multilocus genetically analyzed. The 32 isolates were grouped into two primary lineages resembling the previously characterized Colletotrichum acutatum and Colletotrichum nymphaeae based on the conducted genetic analysis for five genetic loci. The virulence of 16 Colletotrichum spp. strains were evaluated in a detached fruit assay of 10 Greek olive cultivars. The results clearly suggested that fungal isolates belonging to both C. acutatum and C. nymphaeae exhibited different levels of pathogenicity in a cultivar-dependent manner. Thus, cultivars examined in terms of the % Disease Index (%DI) were divided into highly tolerant, tolerant, and susceptible, and those analyzed regarding the % Disease Severity Index (%DSI) were divided into tolerant and susceptible. Our results suggest that the Greek cultivars of Athinolia and Megaritiki are highly tolerant to the vast majority of Colletotrichum strains isolated from Peloponnesian groves and consist of a significant genetic material for the future design of crop protection programs against anthracnose breakouts. [ABSTRACT FROM AUTHOR]

Korla fragrant pear is a high-quality local pear variety native to Xinjiang, China. Currently, the low fruit-setting rate and low calyx shedding rate problems in Korla fragrant pears have been highlighted, which seriously affect the fruit yield and quality. It is of great significance to research the fruiting characteristics and molecular-assisted identification of Korla fragrant pear bud mutation materials for enriching the germplasm resources of Korla fragrant pear. In this research, a natural pollination group (YB) of Korla fragrant pear bud mutation materials and a natural pollination group (CK) of Korla fragrant pears were established. On the fruiting characteristics, the fruit-setting rate and calyx-removal rate of the two groups were investigated. In terms of fruit quality, the fruit shape index, fruit specific gravity, soluble solids content, sugar:acid ratio, soluble sugar content, and other indicators were measured. For the anatomical structure of the calyx tube, the detachment cells were observed. The formation time of the two groups of detached cells was compared. In the GBS simplified genome sequencing, a phylogenetic tree was constructed based on the obtained SNP sites. A principal component analysis, population genetic structure analysis, and genetic diversity index analysis were carried out. In the aspect of SSR molecular marker identification, the SSR types were counted. Polyacrylamide gel electrophoresis was performed. The results demonstrate the following: (1) the fruit-setting rate (30.87%) and calyx-removal rate (68.11%) in the YB group were significantly higher than those in the CK group (19.37%) and the calyx-removal rate (55.18%). (2) There was no significant difference in fruit quality indexes, such as average fruit weight (127.10–130.00 g) and soluble sugar content (9.47–9.56%) between the two groups. (3) Abscission-layer cells were observed at 2, 4, 6, 8, and 10 h after calyx tube discoloration in the YB group and at 48, 72, and 96 h after calyx tube discoloration in the CK group. (4) The genetic background of the YB group and the CK group was similar at the GBS level, but there were differences at the DNA level. This research finally shows that Korla fragrant pear bud mutation material is a good germplasm resource. This germplasm resource can promote the structural optimization of Korla fragrant pear varieties and the healthy development of the industry. [ABSTRACT FROM AUTHOR]

Citrus tristeza virus (CTV) is a globally pervasive and economically significant virus that negatively impacts citrus trees, leading to substantial reductions in fruit yield. CTV occurs within the phloem of infected plants, causing a range of disease phenotypes, such as stem pitting (SP), quick decline (QD), and other detrimental diseases. Research on CTV is challenging due to the large size of its RNA genome and the diversity of CTV populations. Comparative genomic analyses have uncovered genetic diversity in multiple regions of CTV isolates' genomes, facilitating the classification of the virus into distinct genotypes. Despite these challenges, notable advancements have been made in identifying and controlling CTV strains through serological and molecular methods. The following review concentrates on the techniques of nucleic acid identification and serological analysis for various CTV isolates, assisting in the comparison and evaluation of various detection methods, which are crucial for the effective management of CTV diseases, and so contributes to the innovation and development of CTV detection methods. [ABSTRACT FROM AUTHOR]

Despite its significant thermal requirements, melon is a vegetable species that holds the potential for expanding the crop range in temperate climate regions. The selection of appropriate varieties and agronomic practices facilitates its cultivation in these regions. This experiment, employing a randomized block design, was conducted from 2019 to 2021, and this study evaluated the response of three melon varieties—'Seledyn F1', 'Melba', and 'Malaga F1' (factor I)—to various mulching materials (factor II): black polyethylene film (PE), black polypropylene nonwoven (PP), biodegradable film (Fbio), and giant miscanthus straw. Control plots were left unmulched. Melon seeds were sown on 15 April, and seedlings were transplanted on 31 May at a spacing of 100 × 80 cm. This study assessed yield, fruit number, individual fruit weight, and vertical and horizontal fruit diameters. Under optimal conditions, the varieties Seledyn F1 and Malaga F1 produced fruits with the highest individual mass. The application of synthetic mulches led to a two-fold increase in fruit yield compared to unmulched plots, with a 23.7% increase in fruit number. On average, the largest fruits were obtained with PE mulch. Melons grown on Fbio mulch yielded, on average, 40% less and produced 18.8% fewer fruits compared to those grown with PE mulch. [ABSTRACT FROM AUTHOR]

The productivity and quality of strawberries in southern Brazil are affected by the lack of cultivars adapted to different regions and by their susceptibility to diseases. This study aims to evaluate the resistance of cultivars and genotypes to mycosphaerella leaf spot (MLS) and powdery mildew (PM) and the quantitative and qualitative fruit agronomic parameters (FAP) of strawberries in southern Brazil during the 2019/2020 and 2020/2021 growing seasons. The incidence and severity of MLS and PM were evaluated from the beginning of symptoms appearances until harvest. Cultivars and genotypes were compared based on the area under the incidence (AUIDPC) and severity (AUSDPC) disease progress curves and the quantitative and qualitative FAP. The data were subjected to analysis of variance, and multivariate analysis was performed using principal component analysis (PCA). The cultivars Irma, Bellalinda, Kiara, Jonica, and Pircinque exhibited the lowest values of AUIDPC and AUSDPC, demonstrating the highest resistance to MLS and PM. The cultivars Kiara, Jonica, and Pircinque, along with genotype FRF LAM 119.1, showed the best qualitative and quantitative FAP. However, the FRF LAM 119.1 genotype exhibited the highest values of AUIDPC and AUSDPC, indicating high susceptibility to MLS and PM. CPA data indicated that the cultivars Kiara, Jonica, and Pircinque had the highest level of resistance, which was associated with the highest total fruit yield and the best fruit quality in terms of skin color and flavor balance. Kiara, Jonica, and Pircinque proved to be good options for strawberry production in southern Brazil, combining resistance to MLS and PM with good FAP. [ABSTRACT FROM AUTHOR]