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Simple Summary: The insect epidermis covers multiple parts of the insect body, fulfilling both structural and protective roles. Primarily composed of chitin and epidermal proteins, the insect epidermis displays considerable diversity among insects. The CPR family, the largest and most diverse group of cuticular protein (CP) genes identified to date, plays crucial roles in insect development, including body length regulation and cuticle formation. Steroid hormones, such as ecdysteroids, regulate the expression of epidermal proteins. In addition, the insecticide Methoxyfenozide mimics the action of 20E, thereby affecting insect growth. Our study on Heortia vitessoides Moore elucidates the role of HvCP3L and offers novel insights into pest management strategies for Aquilaria sinensis (Lour.) Spreng. Cuticle proteins (CPs) constitute a multifunctional family; however, the physiological role of Cuticle Protein 3-like (CP3L) in Heortia vitessoides Moore remains largely unclear. In this study, we cloned the HvCP3L gene from the transcriptional library of Heortia vitessoides Moore. RT-qPCR results revealed that HvCP3L exhibited high expression levels during the larval stage of Heortia vitessoides Moore, particularly at the L5D1 stage, observed in both larval and adult heads. Through RNA interference, we successfully silenced the HvCP3L gene, resulting in a significant reduction in the survival rate of Heortia vitessoides Moore, with the survival rate from larvae to adults plummeting to a mere 17.7%, accompanied by phenotypic abnormalities. Additionally, we observed that the knockdown of HvCP3L led to the inhibition of genes in the chitin pathway. Following exposure to methoxyfenozide stress, the HvCP3L gene exhibited significant overexpression, coinciding with phenotypic abnormalities. These findings underscore the pivotal role of HvCP3L in the growth and development of Heortia vitessoides Moore. [ABSTRACT FROM AUTHOR]

Simple Summary: Heortia vitessoides Moore is a leaf-eating pest that affects Aquilaria sinensis. In the outbreak period, the leaves of Aquilaria sinensis can be eaten up in a short time, resulting in the death of trees and great economic losses. Chitin is the main component of insect cuticle, peritrophic membrane, and tracheal intima. Chitin synthesis in insects is a complex process that requires the cooperation of many enzymes. Chitin synthase is one of the key enzymes in the process. Chitin synthase is divided into two types: chitin synthase A gene (Chsa) and chitin synthase B gene (Chsb). Chsb is mainly responsible for the tissue synthesis of chitin in midgut peritrophic membrane. It was found that the expression of HvChsb was inhibited, the growth and development were abnormal, and the mortality rate was increased. These findings provide a reference for the prevention and control of the pest from the perspective of gene manipulation. The chitin synthase B gene is a key enzyme in the chitin synthesis of insect peritrophic matrix (PM), which affects insects' feeding behavior. The chitin synthase B gene was cloned from the transcription library of Heortia vitessoides Moore. RT-qPCR showed that HvChsb was highly expressed in the larval stage of H. vitessoides, especially on the first day of the pre-pupal stage, as well as in the midgut of larvae and the abdomen of adults. After starvation treatment, HvChsb was found to be significantly inhibited over time. After 48 h of starvation, the feeding experiment showed that HvChsb increased with the prolongation of the re-feeding time. The experimental data showed that feeding affected the expression of HvChsb. HvChsb was effectively silenced via RNA interference; thus, its function was lost, significantly decreasing the survival rate of H. vitessoides. The survival rate from larval-to-pupal stages was only 43.33%, and this rate was accompanied by abnormal phenotypes. It can be seen that HvChsb plays a key role in the average growth and development of H. vitessoides. [ABSTRACT FROM AUTHOR]

In the current study, corn stalk rinds were used as feedstock for the production of solid-fuel pellets. In an effort to comprehensively analyze the effects of different operational parameters on the physical properties of pellets, response surface methodology (RSM) was employed in conjunction with a Box–Behnken experimental design (BBD). By assessing multiple variables simultaneously and examining their interactions, BBD facilitates the development of a reliable response model that can predict how changes in independent variables will impact response variables. The recorded responses included relaxed density, mechanical durability, and compressive strength. Based on the results, greater R2 values of 0.9467, 0.8669, and 0.9196, could be, respectively, attained for the quadratic regression models. The analysis of variance revealed that all independent variables had significant effects on the responses. The optimal processing condition for the pellets was established by determining the ideal combination of operational parameters. The process entailed the choice of a particle dimension measuring 0.5 mm, a moisture level of 11.35%, the application of heat at 125.7 °C on the die, and the utilization of a molding pressure of 154.2 MPa. Based on these factors, the predicted response values were determined to be 1639.61 kg/m3 for relaxed density, 97.95% for mechanical durability, and 10.18 MPa for compressive strength. The values obtained experimentally under the optimized conditions were similar to the predicted values with a desirability value of 1.00. [ABSTRACT FROM AUTHOR]

In order to reduce the noble metal palladium dosage in the preparation of CL-20 so as to reduce its production cost, several carbon supports were prepared successfully using the hydrothermal carbonization method in the absence or presence of urea using microcrystalline cellulose (MC) as the carbon source, and the corresponding Pd(OH)2/C catalysts were fabricated using the deposition–precipitation method, which showed high activity in the debenzylation reaction of hexabenzylhexaazaisowurtzitane (HBIW) and tetraacetyldibenzylhexaazaisowurtzitane (TADB). It was found that all the catalysts showed a high efficiency in the debenzylation of HBIW, indicating that the structure of the used carbon supports had a limited impact on the catalyst performance in this reaction. On the contrary, the activities of the catalysts in the debenzylation of TADB were quite different. The results of the nitrogen sorption isotherm measurement (BET), scanning electron microscope (SEM), scanning transmission electron microscopy (STEM), powder X-ray diffraction (XRD), element analysis and temperature programmed desorption (TPD), as well as X-ray photoelectron spectra (XPS) characterizations of the supports and catalysts, showed that the relatively high activity of Pd/HTC in the debenzylation of TADB was related to the high Pd dispersion and novel mesoporous structure, while the further higher activity and stability of Pd/HTC-N1:1 in the same reaction were related with its high Pd dispersion, high total oxidized Pd species, and high surface pyridinic N contents as well as the eggshell distribution of Pd species on the support. [ABSTRACT FROM AUTHOR]

D. magna can affect the composition of planktonic bacteria, and can also significantly inhibit the growth of Cyanobacteria at high density. In this study, the inhibitory effects of low-density D. magna groups were stronger than high-density groups due to increases in Acidobacteria abundance in water. Meanwhile, D. magna can inhibit the growth of Planktothrix and Microcystis, but especially the growth of the latter. Alternatively, M. aquaticum and C. demersum can change the community structure of planktonic bacteria. Among them, the inhibitory effect of M. aquaticum on Microcystis and Planktothrix is strong, but it also increases the relative abundance of Chlamydia in water and the risk of pathogenic bacteria. In contrast, the inhibitory effect of C. demersum was more significant on Microcystis than on Planktothrix. Therefore, the combination of "submerged plants-Daphnia", especially the combination of low density D. magna with M. aquaticum, had a significant inhibitory effect on Planktothrix and Microcystis. [ABSTRACT FROM AUTHOR]

The effect of microwave coupled hot air drying on rehydration ratio (RR) and total sugar content (TSC) of Chinese yam was investigated. Single factor test and response surface methodology were used for process parameter optimization with hot air temperature, hot air velocity, slice thickness, and microwave power density as variables and RR and TSC of dried products as responses. The effect of variables on RR followed the order: slice thickness > hot air temperature > microwave power density > hot air velocity. The effect of variables on TSC followed the order: slice thickness > microwave power density > hot air velocity > hot air temperature. The optimized process parameters were hot air velocity of 2.5 m/s, hot air temperature of 61.7 °C, slice thickness of 8.5 mm, and microwave power density of 5.9 W/g. Under the optimal conditions, the predicted values of RR and TSC were 1.90 g/g and 5.74 g/100 g, respectively, which is very close to corresponding actual values (1.83 g/g and 5.72 g/100 g). The desirability of 0.913 further validated the effectiveness of the model. The findings from this work may apply to other agricultural products. [ABSTRACT FROM AUTHOR]