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Simple Summary: Advances in pain research challenge the concept that animals lack pain senses, showing that they have similar neural pathways to humans and experience pain similarly. Understanding brain circuits for effective pain control is crucial for adjusting pain control to individual patient responses and conditions. Pain management in oncological patients aims to lessen the impact of tumor cell development and its consequences on the immune system. Researchers have focused on improving algological approaches to better respond to patient needs, which requires a deeper understanding of how analgesics work, interact with other drugs, and affect patients' conditions. Opioids, although linked to tumor progression, remain the mainstay for managing oncologic pain. Advancements in understanding pain physiopathology have historically challenged animals' absence of pain senses. Studies have demonstrated that animals have comparable neural pain pathways, suggesting that cats and dogs likely experience pain similarly to humans. Understanding brain circuits for effective pain control has been crucial to adjusting pain management to the patient's individual responses and current condition. The refinement of analgesic strategies is necessary to better cater to the patient's demands. Cancer pain management searches to ascertain analgesic protocols that enhance patient well-being by minimizing or abolishing pain and reducing its impact on the immune system and cancer cells. Due to their ability to reduce nerve sensitivity, opioids are the mainstay for managing moderate and severe acute pain; however, despite their association with tumor progression, specific opioid agents have immune-protective properties and are considered safe alternatives to analgesia for cancer patients. [ABSTRACT FROM AUTHOR]

Simple Summary: Staphylococcus pseudintermedius is a common bacterium that causes skin infections in dogs and can occasionally infect humans, posing a public health risk. The presence of the mecA gene in this bacterium can lead to resistance against methicillin (oxacillin) and other beta-lactam antibiotics, which limits treatment options. This study investigated the characteristics of oxacillin-resistant and oxacillin-susceptible mecA-positive S. pseudintermedius isolates from the skin and nasal cavities of dogs with pyoderma. We found a high prevalence of multidrug resistance and significant genetic diversity among the isolates, both within the dog itself and among others, highlighting the complexity of S. pseudintermedius colonization and infection dynamics in pyoderma dogs. Careful monitoring and treatment strategies are essential to manage the spread of antibiotic resistance effectively. Understanding the epidemiology of mecA-positive Staphylococcus pseudintermedius strains, including those that are oxacillin-susceptible but potentially inducible to resistance, is crucial for developing effective treatment strategies and mitigating public health risks. This study characterized 87 mecA-positive S. pseudintermedius isolates obtained from skin lesions and nasal orifices of 46 dogs with pyoderma enrolled at a referral hospital in Thailand between 2019 and 2020. All isolates underwent antibiogram profiling, SCCmec typing, and pulsed-field gel electrophoresis (PFGE) for phenotypic and genetic analysis. Among the 87 isolates, 33 isolates (37.9%) recovered from 15 dogs were oxacillin-resistant (OR-MRSP), while 54 isolates (62.1%) from 31 dogs were oxacillin-susceptible (OS-MRSP). All OR-MRSP isolates exhibited multidrug resistance (MDR), and 44% of the OS-MRSP isolates also showed MDR. SCCmec typing revealed type V as predominant among OR-MRSP isolates (69.7%), while many oxacillin-susceptible isolates (70.4%) were non-typeable. The OR-MRSP isolates from the same dog showed consistent antibiogram and SCCmec types, while OS-MRSP isolates displayed both identical and diverse patterns. No dominant pulsotypes were observed among the OR-MRSP or OS-MRSP strains. Genetic diversity was also noted among the isolates within the same dogs and among the others, highlighting the complexity of S. pseudintermedius colonization and infection dynamics in pyoderma-affected dogs. [ABSTRACT FROM AUTHOR]

Simple Summary: For more than a decade, researchers have been gathering evidence suggesting pregnancy may alter the resistance of women to the development of diseases later in life. We wondered if parity—pregnancy resulting in live offspring—could enhance or diminish resistance of bitches to subsequent non-reproductive disease conditions, a hypothesis previously untested in domestic dogs. To pursue this goal, we constructed detailed lifetime reproductive and medical histories from 65 purebred Rottweiler bitches enrolled in an exceptional longevity study. An advantage of studying these bitches was that we could conduct a long-term evaluation of the relationship between parity and the lifelong occurrence of a commonly diagnosed degenerative disease, cranial cruciate ligament (CCL) rupture. Notably, we found parous bitches had a significantly lower likelihood of CCL rupture than nulliparous bitches. This 5.5-fold lower incidence of CCL rupture among parous females could not be explained by differences in diet, physical activity, overweight body condition, or reason for nulliparity. Our work may guide future mechanistic studies on the apparent association between parity and CCL rupture resistance. The research may also stimulate investigations into other non-reproductive health outcomes, such as autoimmune diseases, whose occurrence later in life may be impacted by parity. Emerging evidence in women supports the notion that pregnancy may reset disease resistance, thereby providing protection against subsequent adverse health outcomes, but this hypothesis has not been adequately explored in domestic dogs. Cranial cruciate ligament (CCL) rupture is a degenerative orthopedic disease that frequently affects pet dogs, and its risk has been associated with disruption of the reproductive hormone axis. Our research team is conducting a lifetime cohort study of purebred Rottweilers in North America that have lived 30% longer than breed-average. Detailed medical and reproductive histories of 33 nulliparous and 32 parous Rottweilers were generated from questionnaires and review of medical records. Interviews with owners of bitches in the nulliparous group served to limit selection bias, confirming that in no instance was the reason for nulliparity based upon the owner's suspicion that a bitch had a heightened risk for CCL rupture. The risk of CCL rupture associated with parity and other exposure variables was estimated using multivariate logistic regression. Overall, CCL rupture was diagnosed in 17 of 65 (26%) bitches. Median age at first litter and CCL rupture were 3.6 and 6.5 years, respectively. Compared to nulliparous, parous bitches had a significant 94% reduction in CCL rupture risk adjusted for duration of ovary exposure, overweight body condition, dietary pattern, habitual physical activity, and work/sport activity [ORadjusted (95% CI) = 0.06 (0.01–0.46); (p = 0.006)]. The observed parity-associated CCL rupture risk reduction remained robust in sensitivity analysis excluding six nulliparous bitches for which decision not to breed was based on diagnosis of hip or elbow dysplasia, conditions which may be genetically linked to CCL rupture [ORadjusted (95% CI) = 0.08 (0.01–0.58); (p = 0.01)]. This work sets the stage for replication studies in other canine populations that should begin to explore the mechanistic basis for parity-associated CCL rupture risk reduction and to pursue other non-reproductive health outcomes in bitches whose incidence or severity may be parity-sensitive. [ABSTRACT FROM AUTHOR]

Simple Summary: This study investigates the reasons behind the relinquishment of companion animals, specifically cats and dogs, to an open-admission shelter in the US. We analyzed 2836 randomly selected owner relinquishment records over a six-year period (2018–2023) using content analysis, a systematic method for categorizing qualitative data. The most common reasons identified for relinquishment included behavioral issues (28%), housing/moving (18%), and the inability to care for the animals (16%). Aggression, social conflict, and soiling were the most common behavioral-issue reasons reported. However, differences were observed in the pattern of relinquishment reasons based on statistical analyses of species, type of relinquishment, and year. The study found no significant increase in relinquishments due to financial or housing pressures during the peri-pandemic period, but there was an increase in relinquishments due to owners being otherwise unable to care for their pets in 2022 and 2023. This research provides valuable insights for animal welfare organizations and policymakers to develop strategies to better understand animal relinquishment and improve the welfare of both animals and their owners. The relinquishment of companion animals to shelters presents significant challenges for animal welfare organizations and has substantial implications for the well-being of both the animals and their owners. This study aims to investigate the reasons for voluntarily relinquishing animals to shelters, to examine species-specific differences (cats and dogs), to compare initial relinquishments to returns (re-relinquishments or readmissions), and to identify potential changes over a 6-year period framing the onset of the COVID-19 pandemic. A retrospective analysis of owner relinquishment records from an open admission shelter in the US was conducted using content analysis, a novel approach in this area of research. A total of 13 relinquishment reason categories were identified, as well as 9 separate categories for specific behavioral issues. Among 2836 randomly selected records, the most frequent reasons cited were Behavior Issues (28%), Housing/Moving (18%), Unable to Care (16%), Too Many Pets (10%), Financial (6%), and Owner Allergies (5%). The most common behavioral reasons reported were Aggression (32% of behavioral surrenders), Social Conflict (28%), and Soiling (13%). However, differences were observed in the pattern of relinquishment reasons based on statistical analyses of species, type of relinquishment, and year. Regarding temporal trends, Housing/Moving and Financial reasons were not found to have changed significantly since the pandemic, but the relative frequency of the category Unable to Care increased significantly in 2022 and 2023. Collectively, these findings partially replicate those from research spanning the past several decades in this area that has employed less systematic methodology and can further be used to help identify and understand the primary drivers of owner relinquishments. [ABSTRACT FROM AUTHOR]

Simple Summary: Factors affecting dog owners' compliance with veterinary medication recommendations were examined, and barriers and aids to administering medications were identified. Findings revealed that older dogs had more compliant owners, and clients who used no aids for medication were more likely to be compliant. Almost half of owners reported not being shown how to administer the medication, and one-third faced challenges, mainly due to resistant pets. Nearly half of the surveyed clients were noncompliant, which is higher than previously reported in other studies. Our study highlights the necessity of enhancing client compliance with veterinary medication instructions for dogs. It emphasizes the importance of providing standardized instructions and guidance, including demonstrating how to administer medications. Ensuring that prescribed oral medications are palatable and easy to administer before patient discharge may contribute to improved patient outcomes. Client compliance with prescribed medication instructions to treat their pets is a concern. This study describes factors associated with the noncompliance of dog owners with veterinary recommendations for medication, as well as client-reported barriers and aids to administering medications. A cross-sectional survey of dog owners' compliance with veterinary medication recommendations was performed from 9 January 2019 to 18 July 2020. A convenience sample of owners who prescribed medication for their dogs during or following elective veterinary examination was surveyed regarding medication administration experience and compliance. Owners were followed up to determine if the course of medication had been completed. Compliance data were analyzed descriptively. Logistic regression was performed with compliance as the outcome. Medication noncompliance was recorded for 47% (71/151) of owners. Increasing dog age was associated with better owner compliance (p < 0.05). Pet owners who used "nothing" as an aid to medicating were less likely to be noncompliant (p < 0.05). Forty-seven percent (71/151) of owners reported that "nobody" showed them how to administer the medication. One-third of dog owners (47/151) reported challenges in medicating their pets. The most common reason cited by clients reporting challenges was a resistant pet. Demonstration of medication administration techniques and discussion about available aids to medicating a pet may improve client compliance. [ABSTRACT FROM AUTHOR]

Simple Summary: Pulmonary hypertension (PH) is a common and severe complication in dogs infected with Dirofilaria immitis, often persisting even after treatment. This study aimed to evaluate the progression of PH in dogs by assessing changes in radiographic parameters and the Right Pulmonary Artery Distensibility (RPAD) Index before and after treatment. Parameters were measured on the day of diagnosis (Day 0), at discharge (Day 90), and six months post-discharge (Day 270). The results indicated that in non-hypertensive dogs, the RPAD Index significantly improved following treatment. In contrast, hypertensive dogs exhibited a persistently low RPAD Index throughout the study, indicating ongoing PH. Additionally, hypertensive dogs showed consistently elevated VHS, CrPA/R4, and CdPA/R9 ratios compared to non-hypertensive dogs. These findings underscore the persistence of PH despite treatment, suggesting that regular radiographic monitoring of VHS, CrPA/R4, and CdPA/R9 ratios is crucial for assessing and managing long-term outcomes in dogs with heartworm disease. Pulmonary hypertension (PH) is a prevalent and severe complication in dogs infected with Dirofilaria immitis. This study aimed to elucidate the progression of PH by analyzing radiographic parameters and the Right Pulmonary Artery Distensibility (RPAD) Index at three key time points: diagnosis (day 0), discharge (day 90), and six months post-discharge (day 270). Fifty-two heartworm-infected dogs were divided into two groups: non-hypertensive and hypertensive. Radiographic measurements, including Vertebral Heart Size (VHS), CrPA/R4 ratio, and CdPA/R9 ratio, along with the RPAD Index, were assessed on Days 0, 90, and 270. Results indicated that, in Group A, the RPAD Index improved significantly from 42% on Day 0 to 43.16% on Day 90, with no significant change by Day 270 (42%). In contrast, hypertensive dogs exhibited a persistently low RPAD Index, averaging 17% throughout this study (p < 0.001). Radiographic parameters in hypertensive dogs showed continuous elevation compared to non-hypertensive dogs, with significant increases in VHS, CrPA/R4, and CdPA/R9 ratios on day 270 compared to day 0 (p < 0.05). The results confirmed that PH persisted in dogs with D. immitis after adulticide treatment, highlighting the importance of regular radiographic monitoring for assessing and managing long-term outcomes in dogs with PH during and after adulticide treatment. Continuous surveillance is thus essential for the effective post-treatment management of PH in dogs. [ABSTRACT FROM AUTHOR]

Simple Summary: In daily veterinary practice, canine otitis externa is a common presentation and, therefore, many studies were conducted to evaluate predispositions. This study on the other hand aims to evaluate factors that have potentially statistically reduced risks for developing otitis externa. Regarding the results, Rhodesian Ridgebacks and Collies have shown significantly low odds for otitis externa. Furthermore, regarding dogs with endocrinopathies, it was shown that those who are overweight seem to develop otitis externa more likely than those with a normal weight. Especially the latter result should be used in communication with pet owners to point out the importance of strict weight management and, therefore, increasing the quality of life of their dogs. Background: Canine otitis externa is a common presentation in small animal practice. The clinical signs vary individually from patient to patient. Regardless of the expression, they all decrease the quality of life. Therefore, this study aimed to identify factors that showed reduced odds for otitis externa. Methods: Clinical case records were searched for key terms regarding canine otitis. Statistical analyses were applied to evaluate associations with otitis externa. p-values of <0.05 were considered statistically significant. Results: Rhodesian Ridgeback and dogs with underlying infectious diseases had a reduced risk for otitis externa, while Retrievers and dogs with allergies had an increased risk. Furthermore, in the group of dogs with endocrinopathies, those with normal weight showed a reduced risk of developing canine otitis in comparison to those which were overweight. Conclusions: These results show a new point of view to reduce the prevalence of canine otitis by reducing the animals' weight due to an additional risk of otitis in obese or overweight dogs. Furthermore, for the first time, Rhodesian Ridgebacks were identified in our study population to have a lower risk of developing otitis externa. Thus, breeders should reflect—within other responsibilities for the quality of life in their offspring—on breed-specifics about otitis when planning ahead. [ABSTRACT FROM AUTHOR]

Simple Summary: This research aims to address the significant knowledge gap regarding microsatellite instability (MSI) in canine cancers. While MSI has been extensively studied in human oncology, its prevalence and importance in canine tumors remain largely unexplored. We seek to provide a comprehensive analysis of MSI across various canine cancer types using a large dataset of whole-exome sequencing samples. By elucidating the landscape of MSI in canine cancers, the study aims to uncover potential implications for cancer development, progression, and treatment strategies. The findings from this research may significantly impact the veterinary oncology community by identifying new biomarkers for prognosis and treatment response, particularly in relation to immunotherapy approaches. Moreover, the study's novel "MSI-burden" score and its correlation with tumor mutational burden could provide valuable insights into canine cancer biology. Ultimately, this research may open new avenues for targeted therapies and personalized medicine in veterinary oncology, potentially improving outcomes for canine cancer patients. Microsatellite instability (MSI) is a crucial feature in cancer biology, yet its prevalence and significance in canine cancers remain largely unexplored. This study conducted a comprehensive analysis of MSI across 10 distinct canine cancer histotypes using whole-exome sequencing data from 692 tumor-normal sample pairs. MSI was detected in 64% of tumors, with prevalence varying significantly among cancer types. B-cell lymphomas exhibited the highest MSI burden, contrasting with human studies. A novel "MSI-burden" score was developed, correlating significantly with tumor mutational burden. MSI-high (MSI-H) tumors showed elevated somatic mutation counts compared to MSI-low and microsatellite stable tumors. The study identified 3632 recurrent MSI-affected genomic regions across cancer types. Notably, seven of the ten cancer types exhibited MSI-H tumors, with prevalence ranging from 1.5% in melanomas to 37% in B-cell lymphomas. These findings highlight the potential importance of MSI in canine cancer biology and suggest opportunities for targeted therapies, particularly immunotherapies. The high prevalence of MSI in canine cancers, especially in B-cell lymphomas, warrants further investigation into its mechanistic role and potential as a biomarker for prognosis and treatment response. [ABSTRACT FROM AUTHOR]

Simple Summary: The performance of search dogs is limited by their ability to cool down their body because the main cooling ability—panting—contradicts sniffing. We investigated the effect of hot environments on the general body condition of detection dogs working in Kenya. These dogs search for cheetah scats in their arid and hot habitat, where it is especially challenging for them to work. After a working day in the fields, these dogs showed a softer stool, which means a higher stress level, and elevated body temperature during the routine check-in the next morning. Our results showed that the use of search dogs in hot conditions is possible and useful but requires increased attention to prevent heat-related illness. Body temperature is an important physiological parameter that influences the performance of working dogs. The main cooling mechanism in dogs is panting to support evaporative cooling, which reduces the dog's ability to detect scents. In this study, we investigated the general body condition of four detection dogs searching for cheetah scats in a hot environment in northern Kenya. We evaluated the effect on the dog's body temperature post-work in the short term (within hours) and long term (12–24 h). The fecal consistency and mean body temperature of the investigated dogs differed significantly between individuals but not between locations (moderate Nairobi and hot Samburu). On the morning after fieldwork, the dogs showed a significantly increased body temperature (37.9 ± 0.8 °C) compared to resting days (37.5 ± 2.2 °C). In the short term, on the first day of fieldwork, the dog's body temperature (n = 2) decreased after 10 min of rest. On the second consecutive day of fieldwork, the 10-min recovery period was too short, and the body temperature did not decrease significantly. Our data showed that the use of detection dogs in hot conditions is possible and useful but requires increased attention to prevent heat-related illness. [ABSTRACT FROM AUTHOR]

Simple Summary: The main components of dogs' species-specific behavior are thought to be mainly influenced by domestication. This implies that these phenotypes are expected to be present almost uniformly across the vast number and variety of dogs. However, the evolution of dogs did not end with domestication, as humans subsequently selected them for distinctly different tasks and levels of interactivity with their handlers. We hypothesized that 'cooperative' dog breeds would also show higher levels of dependency toward humans in a difficult problem-solving task than those dogs that were selected for being independent problem solvers. Our target behavior was 'looking back at the human', which is a typical reaction of socialized dogs to a difficult task. Indeed, cooperative dogs performed more gaze alternations between the reward and the nearby humans, and they looked back more than the independent dog breeds did. Importantly, the results cannot be explained by different levels of persistence between the breed types. Functional selection in the recent past of dogs can be considered as an excellent basis for biologically relevant explanations for the breed-level variability in dog behavior. It is still largely unknown to what extent domestication, ancestry, or recent functional selection are responsible for the behavioral differences in whether dogs look back to a human when presented with a difficult task. Here, we tested whether this ubiquitous human-related response of companion dogs would appear differently in subjects that were selected for either cooperative or independent work tasks. We tested N = 71 dogs from 18 cooperative and 18 independent breeds. Subjects learned in a five-trial warming-up phase that they could easily obtain the reward from a container. In trial six, the reward became impossible to take out from the locked container. When the task was easy, both breed groups behaved similarly, and their readiness to approach the container did not differ between the last 'solvable' and the subsequent 'unsolvable' trial. Task focus, looking at the container, touching the container for the first time, or interacting with the container with a paw or nose did not differ between the breed groups, indicating that their persistence in problem solving was similar. However, in the 'unsolvable' trial, cooperative dogs alternated their gaze more often between the container and the humans than the independent dogs did. The frequency of looking back was also higher in cooperative dogs than in the independent breeds. These are the first empirical results that suggest, in a balanced, representative sample of breeds, that the selection for different levels of cooperativity in working dogs could also affect their human-dependent behavior in a generic problem-solving situation. [ABSTRACT FROM AUTHOR]

Simple Summary: Pain scoring in dogs can be challenging, particularly in a busy clinical setting and when staff with diverse training and veterinary students are involved. Consequently, concerns about dogs receiving inadequate analgesia were raised at this institution. This study was performed to investigate if veterinary students, veterinary nurses, veterinary surgeons with no specific training in anaesthesia, and diplomates in veterinary anaesthesia and analgesia perform pain scoring using the short form of the canine Glasgow Composite Measure Pain Scale (CMPS-SF) in a similar manner. The results obtained indicated good to excellent pain assessment agreement between groups. Nevertheless, the overall agreement amongst all assessors was poor and the intra-group agreement was poor to moderate, suggesting significant individual differences when pain scoring dogs. Veterinary students tend to pain score higher than more experienced assessors. Several pain scoring systems have been validated to measure pain in dogs. However, pain may not be adequately assessed since these tools are associated with high-level inter-observer variation. The aim of this study is to evaluate the agreement of pain assessment using the CMPS-SF between veterinary students, veterinary nurses, veterinary surgeons, and European College of Veterinary Anaesthesia and Analgesia (ECVAA) diplomates. Forty-five client-owned dogs presented to a teaching hospital were enrolled in this prospective, observational study. All dogs were pain-scored in vivo, while a video of the assessment was recorded and subsequently evaluated by twenty assessors, with five per group. Mean scores between groups were compared, and agreement within groups and agreement of the average scores between groups were assessed by calculating the intraclass correlation coefficient (ICC). The intervention point at which dogs were deemed to require additional analgesia was also evaluated. Overall agreement of pain assessment was poor (ICC = 0.494). Nurses had the best inter-observer agreement (ICC = 0.656), followed by ECVAA diplomates (ICC = 0.540), veterinary surgeons (ICC = 0.478), and veterinary students (ICC = 0.432). The best inter-group agreement was between veterinary surgeons and nurses (ICC = 0.951) and between ECVAA diplomates and nurses (ICC = 0.951). Students were more likely to determine that additional analgesia was required compared to other groups. Pain assessment is key for animal welfare, and training in this area should be reinforced to improve consistency. [ABSTRACT FROM AUTHOR]

Although therapies based on mesenchymal stromal cells (MSCs) are being implemented in clinical settings, many aspects regarding these procedures require further optimization. Domestic dogs suffer from numerous immune-mediated diseases similar to those found in humans. This study aimed to assess the immunomodulatory activity of canine (c) Wharton jelly (WJ)-derived MSCs and refer them to human (h) MSCs isolated from the same tissue. Canine MSC(WJ)s appeared to be more prone to in vitro aging than their human counterparts. Both canine and human MSC(WJ)s significantly inhibited the activation as well as proliferation of CD4+ and CD8+ T cells. The treatment with IFNγ significantly upregulated indoleamine-2,3-dioxygenase 1 (IDO1) synthesis in human and canine MSC(WJ)s, and the addition of poly(I:C), TLR3 ligand, synergized this effect in cells from both species. Unstimulated human and canine MSC(WJ)s released TGFβ at the same level (p > 0.05). IFNγ significantly increased the secretion of TGFβ in cells from both species (p < 0.05); however, this response was significantly stronger in human cells than in canine cells. Although the properties of canine and human MSC(WJ)s differ in detail, cells from both species inhibit the proliferation of activated T cells to a very similar degree and respond to pro-inflammatory stimulation by enhancing their anti-inflammatory activity. These results suggest that testing MSC transplantation in naturally occurring immune-mediated diseases in dogs may have high translational value for human clinical trials. [ABSTRACT FROM AUTHOR]

Most existing studies on pet breeds classification focus on images with simple backgrounds, leading to the unsatisfactory performance of models in practical applications. This paper investigates training pet breeds classification models using complex images and constructs a dataset for identifying breeds of pet cats and dogs. We use this dataset to fine-tune three SOTA models: ResNet34, DenseNet121, and Swin Transformer. Specifically, in terms of top-1 accuracy, the performance of DenseNet is improved from 89.10% to 89.19%, while that of the Swin Transformer is increased by 1.26%, marking the most significant enhancement. The results show that training with our dataset significantly enhances the models' classification capabilities in complex environments. Additionally, we offer a lightweight pet breeds identification model based on PBI-EdgeNeXt (Pet Breeds Identification EdgeNeXt). We utilizes the PolyLoss function and Sophia optimizer for model training. Furthermore, we compare our model with five commonly used lightweight models and find that the proposed model achieves the highest top-1 accuracy of 87.12%. These results demonstrate that the model achieves high accuracy, reaching the SOTA level. [ABSTRACT FROM AUTHOR]

Simple Summary: It is well known that highly inbred dogs are more prone to diseases than less inbred or outbred dogs. This is because inbreeding increases the number of bad mutations present in both paternal and maternal chromosomes (recessive mutations) of the dogs. Using the genome data from 392 dogs belonging to 83 breeds, we investigated the association between the abundance of recessive bad mutations and dog health. The frequency of visits to veterinary clinics for non-routine care was used as the measure of dog health. Our results revealed a highly significant positive relationship between the number of recessive harmful mutations and the degree of dog health. The dog breeds that required more veterinary care had two times higher harmful mutations than those that required less care. The results of this study could be useful for understanding the disease burden on breed dogs and as a guide for dog breeding programs. It is well known that highly inbred dogs are more prone to diseases than less inbred or outbred dogs. This is because inbreeding increases the load of recessive deleterious variants. Using the genomes of 392 dogs belonging to 83 breeds, we investigated the association between the abundance of homozygous deleterious variants and dog health. We used the number of non-routine veterinary care events for each breed to assess the level of morbidity. Our results revealed a highly significant positive relationship between the number of homozygous deleterious variants located within the runs of homozygosity (RoH) tracts of the breeds and the level of morbidity. The dog breeds with low morbidity had a mean of 87 deleterious SNVs within the RoH, but those with very high morbidity had 187 SNVs. A highly significant correlation was also observed for the loss-of-function (LoF) SNVs within RoH tracts. The dog breeds that required more veterinary care had 2.3 times more homozygous LoF SNVs than those that required less veterinary care (112 vs. 50). The results of this study could be useful for understanding the disease burden on breed dogs and as a guide for dog breeding programs. [ABSTRACT FROM AUTHOR]

To understand the large inter-species variations in drug effects on repolarization, the properties of the rapid (IKr) and the slow (IKs) components of the delayed rectifier potassium currents were compared in myocytes isolated from undiseased human donor (HM), dog (DM), rabbit (RM) and guinea pig (GM) ventricles by applying the patch clamp and conventional microelectrode techniques at 37 °C. The amplitude of the E-4031-sensitive IKr tail current measured at −40 mV after a 1 s long test pulse of 20 mV, which was very similar in HM and DM but significant larger in RM and GM. The L-735,821-sensitive IKs tail current was considerably larger in GM than in RM. In HM, the IKs tail was even smaller than in DM. At 30 mV, the IKr component was activated extremely rapidly and monoexponentially in each studied species. The deactivation of the IKr component in HM, DM, and RM measured at −40 mV. After a 30 mV pulse, it was slow and biexponential, while in GM, the IKr tail current was best fitted triexponentially. At 30 mV, the IKs component activated slowly and had an apparent monoxponential time course in HM, DM, and RM. In contrast, in GM, the activation was clearly biexponential. In HM, DM, and RM, IKs component deactivation measured at −40 mV was fast and monoexponential, while in GM, in addition to the fast component, another slower component was also revealed. These results suggest that the IK in HM resembles that measured in DM and RM and considerably differs from that observed in GM. These findings suggest that the dog and rabbit are more appropriate species than the guinea pig for preclinical evaluation of new potential drugs expected to affect cardiac repolarization. [ABSTRACT FROM AUTHOR]

Simple Summary: The benefit of spatially fractionated radiotherapy for brain tumors is maximized through Synchrotron Microbeam Radiation Therapy (MRT). In 2021, the clinical transfer phase of MRT began: the first brain-tumor-bearing dog patients were treated under clinical conditions in view of the forthcoming clinical transfer. As a primary endpoint, the tolerance of normal brain tissues to MRT was evaluated, while the efficacy in reducing tumor volume was considered as a secondary endpoint. We here present acute to subacute neurologic radiotolerance and tumor volume reduction after MRT for brain tumor treatment in canine patients included in our ongoing veterinary trial, proving that MRT is a safe tool for spontaneous brain tumor treatment in dogs. Synchrotron Microbeam Radiation Therapy (MRT) has repeatedly proven its superiority compared with conventional radiotherapy for glioma control in preclinical research. The clinical transfer phase of MRT has recently gained momentum; seven dogs with suspected glioma were treated under clinical conditions to determine the feasibility and safety of MRT. We administered a single fraction of 3D-conformal, image-guided MRT. Ultra-high-dose rate synchrotron X-ray microbeams (50 µm-wide, 400 µm-spaced) were delivered through five conformal irradiation ports. The PTV received ~25 Gy peak dose (within microbeams) per port, corresponding to a minimal cumulated valley dose (diffusing between microbeams) of 2.8 Gy. The dogs underwent clinical and MRI follow-up, and owner evaluations. One dog was lost to follow-up. Clinical exams of the remaining six dogs during the first 3 months did not indicate radiotoxicity induced by MRT. Quality of life improved from 7.3/10 [±0.7] to 8.9/10 [±0.3]. Tumor-induced seizure activity decreased significantly. A significant tumor volume reduction of 69% [±6%] was reached 3 months after MRT. Our study is the first neuro-oncologic veterinary trial of 3D-conformal Synchrotron MRT and reveals that MRT does not induce acute to subacute radiotoxicity in normal brain tissues. MRT improves quality of life and leads to remarkable tumor volume reduction despite low valley dose delivery. This trial is an essential step towards the forthcoming clinical application of MRT against deep-seated human brain tumors. [ABSTRACT FROM AUTHOR]

Simple Summary: The Medog region in southeastern Tibet harbors diverse wildlife, yet our understanding of these mammals' species diversity, distribution, and conservation status remains inadequate. To address this, a camera-trapping survey was conducted in Gedang, Medog, spanning from April 2023 to May 2024. The study revealed 25 mammalian species across five orders and 14 families, including Endangered and Vulnerable species. Notably, the Gongshan muntjac's underrepresentation in the IUCN was addressed, and new insights into the coexistence of Himalayan red pandas and Chinese red pandas were documented. The White-cheeked macaque, Gongshan muntjac, and Himalayan serow were frequently detected with high RAI. However, the high detection of domestic dogs, livestock, and human disturbances may pose threats to wildlife. Conservation efforts are crucial to protect these mammals and maintain ecological balance in the region. The Medog in southeastern Tibet is home to a diverse range of wild animals. However, research on these mammals' species directories, distribution, and conservation status remains insufficient, despite their crucial role in maintaining ecological balance. The study carried out a camera-trapping survey to assess mammal biodiversity and the significance of mammal protection in their natural habitats in Gedang, Medog. Future directions and application prospects of the study for wildlife conservation in the southeastern Tibetan mountains were also discussed. The survey, spanning from April 2023 to May 2024, with 19,754 camera trap days, revealed 25 mammalian species across five orders and 14 families. Among these, four classified as Endangered, five as Vulnerable, two as Near Threatened on the IUCN Red List, nine were categorized as Critically Endangered or Endangered on the Red List of China's Vertebrates, and seven were China's national first-class key protected wildlife. The order Carnivora exhibited the highest diversity, comprising 12 species. Furthermore, the study filled the knowledge gap regarding the underrepresentation of Gongshan muntjac Muntiacus gongshanensis in IUCN and provided new insights into the recorded coexistence of the Himalayan red panda Ailurus fulgens and Chinese red panda Ailurus styani along the Yarlung Zangbo River for the first time, and also documented new upper elevation limits for four large to medium-sized species. Regarding the relative abundance indices (RAI) captured by camera traps, the most prevalent species identified was the White-cheeked macaque Macaca leucogenys, followed by the Gongshan muntjac and Himalayan serow Capricornis thar. The monitoring also captured a number of domestic dogs and livestock, as well as human disturbances. These findings underscore the importance of conserving these mammals and emphasize the need for conservation efforts to protect their habitats and reduce human activities that threaten their survival, thereby maintaining the ecological balance of the region. Additionally, the research highlighted Gedang's significance to global conservation efforts for mammalian diversity, providing essential data for effective wildlife conservation strategies. [ABSTRACT FROM AUTHOR]

The use of tyrosine kinase inhibitors (TKI) has been growing in veterinary oncology and in the past few years several TKI have been tested in dogs. However, different from human medicine, we lack strategies to select patients to be treated with each TKI. Therefore, this study aimed to screen different tumor subtypes regarding TKI target immunoexpression as a predictor strategy to personalize the canine cancer treatment. It included 18 prostatic carcinomas, 36 soft tissue sarcomas, 20 mammary gland tumors, 6 urothelial bladder carcinomas, and 7 tumors from the endocrine system. A total of 87 patients with paraffin blocks were used to perform immunohistochemistry (IHC) of human epidermal growth factor receptor 2 (HER-2), epidermal growth factor receptors 1 (EGFR1), vascular endothelial growth factor receptor 2 (VEGFR-2), platelet derived growth factor receptor beta (PDGFR-β), c-KIT, and extracellular signal-regulated kinase 1/2 (ERK1/ERK2). The immunohistochemical screening revealed a heterogeneous protein expression among histological types with mesenchymal tumors showing the lowest expression level and carcinomas the highest expression. We have demonstrated by IHC screening that HER2, EGFR1, VEGFR-2, PDGFR-β and ERK1/ERK2 are commonly overexpressed in dogs with different carcinomas, and KIT expression is considered relatively low in the analyzed samples. [ABSTRACT FROM AUTHOR]

This study aimed to develop fecal low-odor adult dog feed using processed corn protein powder as the primary raw material and evaluate its effectiveness through feeding experiments. The objectives included analyzing the fundamental nutritional indicators, comparing the quality of fecal low-odor adult dog feed with commercially available dog feed, and assessing the changes in the eating behavior, physical condition, and physiological and biochemical indicators before and after feeding on the fecal low-odor adult dog feed. This study involved formulating dog feed using processed fermented corn protein powder and conducting nutritional analyses to compare moisture, crude protein, ash, crude fat, and starch contents. Physical properties such as hardness and cohesion were also evaluated. Feeding experiments were conducted with adult dogs to monitor the changes in the eating behavior and physical condition. Physiological and biochemical indicators, including ammoniacal nitrogen and putrefactive odor in feces, were measured both before and after feeding to assess the impact of the fecal low-odor adult dog feed. The analysis showed no significant difference in moisture (p > 0.05), crude protein (p > 0.05), ash (p > 0.05), crude fat (p > 0.05), and starch (p > 0.05) contents between the fecal low-odor adult dog feed and commercially available dog feed. Similarly, no significant difference was noted in the hardness (p > 0.05) and cohesion (p > 0.05) between the two types of dog feed. Both types of dog feed contained rich volatile compounds with varying compositions. The commercially available dog feed had a sour odor, whereas the fecal low-odor adult dog feed had a barbecue and resin-like smell. After feeding with the fecal low-odor adult dog feed, the liquid nitrogen content significantly decreased (p < 0.01). Also, the indole content, a major contributor to fecal odor, based on the data, decreased after feeding. [ABSTRACT FROM AUTHOR]

Simple Summary: Soft tissue sarcomas (STSs) are rare tumors that develop from mesenchymal cells and can occur in both humans and dogs. Despite their rarity, STSs are challenging due to their tendency to recur and spread. Dogs represent valuable models for studying human STSs because they develop similar tumors naturally and share environmental risk factors with humans. Current treatments for STSs include surgery, radiation, and chemotherapy, but there is a need for new therapies due to the high failure rates of new drugs. In this study, new therapeutical approaches will be reviewed, since understanding the tumor microenvironment is crucial for developing better treatments, such as immunotherapy, for both dogs and humans. Soft tissue sarcomas (STSs) represent a diverse group of tumors arising from mesenchymal cells, affecting both humans and animals, including dogs. Although STSs represent a class of rare tumors, especially in humans, they pose significant clinical challenges due to their potential for local recurrence and distant metastasis. Dogs, as a model for human STSs, offer several advantages, including exposure to similar environmental risk factors, genetic diversity among breeds, and the spontaneous development of tumors. Furthermore, canine tumors closely mimic the heterogeneity and complexity of human tumors, making them valuable for research into disease progression and treatment effectiveness. Current treatment approaches for STSs in both dogs and humans primarily involve surgery, radiation therapy, and chemotherapy, with treatment decisions based on tumor characteristics and patient factors. However, the development of novel therapeutic strategies is essential, given the high failure rate of new drugs in clinical trials. To better design new tailored treatments, comprehension of the tumor microenvironment (TME) is fundamental, since it plays a crucial role in STS initiation and progression by modulating tumor behavior, promoting angiogenesis, and suppressing immune responses. Notably, TME features include cancer-associated fibroblasts (CAFs), extracellular matrix (ECM) alterations, and tumor-associated macrophages (TAMs) that, depending on their polarization state, can affect immune responses and thus the patient's prognosis. In this review, new therapeutical approaches based on immunotherapy will be deeply explored as potential treatment options for both dogs and humans with STSs. In conclusion, this review provides an overview of the current understanding of STSs in dogs and humans, emphasizing the importance of the TME and potential treatment strategies. [ABSTRACT FROM AUTHOR]

Simple Summary: Canine cutaneous mast cell tumors have variable rates of recurrence and metastasis. We sought to evaluate how various prognostic factors affect survival, recurrence, and metastasis in a retrospective study of 90 dogs with cutaneous mast cell tumors treated with surgery and vinblastine chemotherapy. Factors evaluated included age, breed, grade, margins, local tumor control, mitotic count, lymph node metastasis, response to vinblastine chemotherapy in the gross disease setting, and timing of vinblastine in relation to surgery. Eighteen dogs received neoadjuvant vinblastine, and no dogs progressed locally before surgery. The use of neoadjuvant vinblastine was associated with a higher chance of local recurrence (p = 0.03) but not survival. Significantly shorter survival times were found for high-grade tumors (p < 0.001), grade 3 tumors (p < 0.001), and tumors with a mitotic count of >5 (p < 0.001). Dogs with grade 2/low grade cutaneous mast cell tumors lived longer than those with grade 2/high grade tumors (p < 0.001). Both grading systems and mitotic count were prognostic for survival in this population of dogs, supporting the need for standard reporting of histopathologic findings. Neoadjuvant chemotherapy can be effective in downsizing canine cutaneous mast cell tumors but does not influence survival. Objective: Canine cutaneous mast cell tumors (cMCTs) have variable rates of recurrence and metastasis. We evaluated how various prognostic factors affect survival, recurrence, and metastasis in dogs with cMCT who underwent surgery and vinblastine chemotherapy. Animals: 90 dogs with cMCT treated with surgery and vinblastine at a veterinary referral institution were included. Methods: Medical records were retrospectively reviewed. Prognostic factors were evaluated. Results: Most dogs (94%) had grade 2 or 3 cMCTs. Neoadjuvant vinblastine was used in 18 dogs, and none progressed locally before surgery. The use of neoadjuvant vinblastine was associated with a higher chance of local recurrence (p = 0.03) but not survival. Shorter survival times were found for tumors that were high-grade (p < 0.001), grade 3 (p < 0.001), or a MC of >5 (p < 0.001). Dogs with grade 2 tumors that were low-grade lived longer than those with high-grade tumors (p < 0.001). Histologic tumor-free margins and the ability to achieve local tumor control were not associated with outcome. Clinical Relevance: Both grading systems and MC were prognostic for survival in this population of dogs, supporting the need for the standard reporting of histopathologic findings. Neoadjuvant chemotherapy can be effective in downsizing cMCTs but does not influence survival. These findings are consistent with previous publications, showing the benefits of a more modern population of patients, surgical treatments, and histopathologic assessments. [ABSTRACT FROM AUTHOR]

Simple Summary: This study investigated using a high-quality computed tomography (CT) scanner to aid the diagnosis of upper airway disease in dogs without the need for sedation or anaesthesia. Data from seventeen dogs with breathing problems were included, and were classified into three groups based on their condition and whether they had previously undergone airway surgery. Commonly reported clinical signs included noisy breathing and difficulty exercising. Results showed this imaging technique may provide an efficient and safe technique to evaluate the anatomical structures of the upper airway. Understanding upper airway diseases better can improve treatment outcomes, especially for breeds prone to airway disease, laying a foundation for further advancements in veterinary care. This study investigated using a 160-slice multidetector computed tomography (CT) scanner for conscious image acquisition in dogs with upper airway disease, and describes findings in dogs that had previously undergone soft palate surgery. Seventeen client-owned dogs with upper airway disease were retrospectively reviewed, and classified into three groups: group I, "untreated brachycephalic obstructive airway syndrome (BOAS) patients"; group II, "previously treated BOAS patients"; and group III, "patients with respiratory disease other than BOAS". Data included signalment, clinical history, direct laryngoscopy and endoscopy findings, previous surgeries and CT findings. CT scans in group I revealed overlong and thickened soft palates in all dogs, and signs of laryngeal collapse in four dogs. Patients in group II exhibited normal soft palate lengths, while patients in group III displayed various findings such as nasopharyngeal narrowing and tracheal collapse. Upper airway examinations under general anaesthesia confirmed most CT findings. This study demonstrates the feasibility and value of conscious CT scanning for assessing upper airway diseases in dogs, providing valuable diagnostic information while eliminating the need for chemical immobilisation, thus reducing patient stress and cost. These findings offer new insight into upper airway anatomy in awake patients, especially in brachycephalic breeds, and lay a foundation for future research. [ABSTRACT FROM AUTHOR]

Simple Summary: Toxoplasmosis is a disease caused by the parasite Toxoplasma gondii, which can infect humans and animals, including dogs. Dogs become infected by accidentally ingesting the parasite's oocysts from the environment and eating infected rodents or other small animals. Following infection, dogs develop antibodies against T. gondii. The current study investigated the percentage of dogs living in Greece that have antibodies against the parasite as an indirect way to assess the level of environmental contamination with T. gondii oocysts. Blood samples were collected from 1282 dogs living in both urban and rural regions of Greece, including Attica and Thessaloniki. Overall, 47.6% of the dogs had antibodies against the parasite, indicating they had been exposed to T. gondii. Seropositive dogs were more commonly found in rural areas (53.8%) than in urban areas (43.9%) (p < 0.001). The findings of this study suggest that T. gondii infection is widespread in dogs across Greece, especially in rural regions, and the parasite is highly present in the environment, posing a potential risk for human exposure in these areas. Control measures are necessary to prevent dogs from being infected and to reduce this risk for dog owners. Dog owners should also follow basic hygiene practices, like washing their hands after petting their dogs, to protect themselves. Toxoplasmosis, caused by the protozoan Toxoplasma gondii, is a zoonotic disease that affects various animal species, including dogs, that can serve as sentinels for indirectly estimating the environmental contamination. The current study aimed to determine the seroprevalence of T. gondii IgG and IgM antibodies in dogs across different regions of Greece and assess their living area as a potential risk factor. In total, 1282 blood samples were collected from dogs in urban and rural areas of Greece, including Attica and Thessaloniki. Serum samples were tested for T. gondii-specific IgG and IgM antibodies using an indirect immunofluorescent antibody test. A chi-square test was performed to assess the association between seropositivity for T. gondii and geographical location (urban/rural). The overall T. gondii seroprevalence was 47.6%, while the seroprevalence of IgG and IgM antibodies was 34.3% and 22.2%, respectively. Dogs from rural areas exhibited a significantly higher seroprevalence (53.8%) than those from urban areas (43.9%) (p < 0.001), with the estimated odds ratio being equal to 1.49 (95% CI, 1.18 to 1.65) and the relative risk increased by 22.4%. Dogs in Greece are highly exposed to T. gondii, particularly in rural areas. Measures to prevent canine infections are necessary, and basic hygiene practices, such as hand washing after petting dogs, are required to reduce human infection risk and safeguard public health. [ABSTRACT FROM AUTHOR]

Background: The manifestation of complex trauma results from exposure to severe and repetitive stressors occurring within the caregiver system. Frequently associated with child maltreatment, complex trauma can lead to impairments in multiple domains, including attachment, affect and behavioural regulation, and cognition. Treatments, including Trauma-Focused Cognitive Behavioural Therapy, have been shown to be efficacious, but high attrition rates point to the need for complementary methods that boost client retention and treatment efficacy. This systematic review examines whether dog-assisted therapy has beneficial impacts on the treatment of complex trauma and whether it can decrease treatment attrition among children presenting with complex trauma exposure. Methods: We followed PRISMA guidelines to locate relevant research reports. Seven published research reports matched the inclusion criteria. Results: Dog-assisted therapy may increase the efficacy of standard interventions for complex trauma, although only one study formally assessed treatment retention. Across most studies, there is an overall lack of detailed information on the nature of therapeutic activities involving the dog and how these activities may complement or enhance therapy as usual. Studies were of varying methodological quality, impacting the reliability of findings. Conclusions: Future studies should aim to better describe and justify dog-assisted therapy techniques and evaluate these in comparison with standard evidence-based approaches to the treatment of childhood complex trauma. [ABSTRACT FROM AUTHOR]

Leishmaniosis is a vector-borne disease caused by protozoan parasites of the genus Leishmania, which are zoonotic and have an important impact on animal and public health globally. Between 2009 and 2023, blood samples from domestic dogs with clinical suspicion of leishmaniosis were received from 286 veterinary medical centres throughout mainland Portugal. An enzyme-linked immunosorbent assay (ELISA) was utilised to detect antibodies against Leishmania infantum antigens. Additionally, a complete blood count and tests for total proteins, urea, creatinine and alanine aminotransferase, as well as protein electrophoresis, were also performed. No significant relationship between sex and breed was observed. The age distribution was bimodal, with the highest prevalence of disease occurring at 2–5 years of age and a secondary peak occurring at 6 years or over (p < 0.001). No statistical correlation was observed between creatinine and urea across the ELISA serological groups. In contrast, both the gamma globulin levels (r = 0.45; p < 0.001) and the albumin/globulin ratio (r = −0.36; p < 0.001) exhibited moderate correlations with the ELISA. These findings support recent seroprevalence studies in dogs, with some geographical areas in Northern Portugal exhibiting the highest values, which may be the result of geographical shifts in parasite circulation due to climate change. [ABSTRACT FROM AUTHOR]

Humans can be infected with anthroponotic (Ancylostoma duodenale and Necator americanus) and with zoonotic (Ancylostoma ceylanicum, A. caninum, A. braziliense, and Uncinaria stenocephala) hookworms from dogs. Anthroponotic species are usually thought not to infect dogs. We used the internal transcribed spacer–1 (ITS1) gene in a quantitative PCR to detect anthroponotic and zoonotic hookworm species in fecal samples from 54 children and 79 dogs living in an indigenous community in tropical Northwestern Ecuador. Hookworm DNA was detected in 59.3% of children and 92.4% of dogs. Among samples from children, zoonotic hookworms were detected in 24.1% (A. ceylanicum 14.8%, A. caninum 11.1%, and A. braziliense 1.9%), whilst in dog samples, anthroponotic species were detected in 19.0% (N. americanus 12.4% and A. duodenale 6.3%). Sanger sequencing was performed successfully on 60 qPCR-positive samples (16 from children and 44 from dogs), and consensus sequences were obtained with >98% homology to GenBank references for hookworm spp. Phylogenetic analysis showed a close relationship between anthroponotic and zoonotic Ancylostoma species and no heterogeneity between A. duodenale and A. caninum; in human samples, we found A. ceylanicum but not A. braziliense sequences and we were unable to identify N. americanus in the dog samples. No infections with U. stenocephala were detected. Our data provide evidence for high rates of hookworm infections in indigenous children and dogs in a marginalized rural setting in coastal Ecuador. We also found evidence for potential cross-transmission of hookworm spp. between humans and dogs that represent a potential domestic reservoir for zoonotic and anthroponotic hookworms. [ABSTRACT FROM AUTHOR]

Dermatophytosis, commonly referred to as ringworm, is a common superficial fungal infection in companion animals and humans. Between 2012 and 2023, plucked hair and scraped scale samples from domestic dogs and cats with clinical suspicion of dermatophytosis were collected from 355 veterinary medical centres across mainland Portugal. A total of 4716 animal samples were inoculated onto DERM agar, incubated at 25 °C for up to 4 weeks, and periodically examined macro- and micro-scopically to observe and evaluate fungal growth. Of these, 271 samples were removed due to contaminant fungi. Of the 568 positive cultures, the highest number were from the North (48.1%; 95% CI: 44.0–52.2%) and Centre (32.4%; 95% CI: 28.7–36.4%) regions. Microsporum canis was the most frequently isolated species (63.9%), followed by Trichophyton spp. (20.3%) and Nannizia gypsea (formerly Microsporum gypseum) (8.1%). Felines exhibited a higher frequency (17.4%) compared with dogs (9.1%) (p < 0.001). In dogs, the Yorkshire Terrier, West Highland White Terrier, Miniature Pinscher, Dalmatian and Miniature Schnauzer demonstrated a significant predisposition to dermatophytosis (p < 0.05). In cats, the Persian and Scottish Fold breeds were significantly predisposed (p < 0.05). No significant differences were found between sexes (p > 0.05). These findings underscore dermatophytosis as an increasing public health concern due to its zoonotic and contagious nature, providing comprehensive insights into the epidemiology of dermatophytosis in Portugal. [ABSTRACT FROM AUTHOR]

Differentiating between Bacillus species is relevant in human medicine. Bacillus thuringiensis toxins might be effective against Strongyloides stercoralis, a nematode causing relevant human morbidity. Our first objective was to evaluate genomic and MALDI-TOF identification methods for B. thuringiensis. Our secondary objective was to evaluate a possible negative selection pressure of B. thuringiensis against S. stercoralis. PCR and Sanger were compared to MALDI-TOF on a collection of 44 B. cereus group strains. B. thuringiensis toxin genes were searched on 17 stool samples from S. stercoralis-infected and uninfected dogs. Metagenomic 16S rRNA was used for microbiome composition. The inter-rate agreement between PCR, Sanger, and MALDI-TOF was 0.631 k (p-value = 6.4 × 10−10). B. thuringiensis toxins were not found in dogs' stool. Bacteroidota and Bacillota were the major phyla in the dogs' microbiome (both represented >20% of the total bacterial community). Prevotella was underrepresented in all Strongyloides-positive dogs. However, the general composition of bacterial communities was not significantly linked with S. stercoralis infection. The genomic methods allowed accurate differentiation between B. thuringiensis and B. cereus. There was no association between B. thuringiensis and S. stercoralis infection, but further studies are needed to confirm this finding. We provide the first descriptive results about bacterial fecal composition in dogs with S. stercoralis infection. [ABSTRACT FROM AUTHOR]

Simple Summary: We studied canine mammary tumors to better understand similar human breast cancer using a technique called liquid biopsy, which analyzes blood samples to detect disease, focusing on the detection of tiny particles called small extracellular vesicles. These structures are very interesting because they can carry proteins that may indicate the presence of cancer. In this study, we collected blood from healthy dogs, dogs with benign and malignant CMTs, and those in remission and also with recurrence. We found no differences in the size or amount of the vesicles among the groups but identified specific proteins that could serve as markers for cancer. These proteins could potentially help in the diagnosis, prognosis and monitoring of mammary cancer. (Background). Canine mammary tumors (CMTs) have emerged as an important model for understanding pathophysiological aspects of human disease. Liquid biopsy (LB), which relies on blood-borne biomarkers and offers minimal invasiveness, holds promise for reflecting the disease status of patients. Small extracellular vesicles (SEVs) and their protein cargo have recently gained attention as potential tools for disease screening and monitoring. (Objectives). This study aimed to isolate SEVs from canine patients and analyze their proteomic profile to assess their diagnostic and prognostic potential. (Methods). Plasma samples were collected from female dogs grouped into CMT (malignant and benign), healthy controls, relapse, and remission groups. SEVs were isolated and characterized using ultracentrifugation (UC), nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM). Proteomic analysis of circulating SEVs was conducted using liquid chromatography–mass spectrometry (LC–MS). (Results). While no significant differences were observed in the concentration and size of exosomes among the studied groups, proteomic profiling revealed important variations. Mass spectrometry identified exclusive proteins that could serve as potential biomarkers for mammary cancer. These included Inter-alpha-trypsin inhibitor heavy chain (ITIH2 and ITI4), phosphopyruvate hydratase or alpha enolase (ENO1), eukaryotic translation elongation factor 2 (eEF2), actin (ACTB), transthyretin (TTR), beta-2-glycoprotein 1 (APOH) and gelsolin (GSN) found in female dogs with malignant tumors. Additionally, vitamin D-binding protein (VDBP), also known as group-specific component (GC), was identified as a protein present during remission. (Conclusions). The results underscore the potential of proteins found in SEVs as valuable biomarkers in CMTs. Despite the lack of differences in vesicle concentration and size between the groups, the analysis of protein content revealed promising markers with potential applications in CMT diagnosis and monitoring. These findings suggest a novel approach in the development of more precise and effective diagnostic tools for this challenging clinical condition. [ABSTRACT FROM AUTHOR]

Simple Summary: Similar to humans, dogs could suffer a syndrome similar to Attention-Deficit/Hyperactivity Disorder (ADHD). Several studies suggest that dogs can serve as a model for studying ADHD. This condition leads to behavioral issues like impulsivity, attention problems, hyperactivity, and sometimes aggression, affecting both the dog's and their caregiver's quality of life. ADHD-like behavior in dogs is linked to disruptions in neurotransmitters like serotonin and dopamine, similar to humans. It seems to result from a mix of genetics and environment. In addition to the mentioned symptoms, dogs with ADHD-like behavior may also display compulsive behaviors, aggression, inappropriate elimination, fearfulness, epilepsy, foreign body ingestion, and itchiness. While there is no clear veterinary diagnosis for ADHD-like behavior in dogs, validated questionnaires can be helpful, but these cannot be used as a unique diagnostic tool. Treatment may involve medications like fluoxetine, along with environmental enrichment, relaxation techniques, and behavior modification to improve the dog's and caregiver's quality of life. Similar to humans, dogs could suffer an Attention-Deficit/Hyperactivity Disorder-like syndrome (ADHD-like). In fact, several studies highlight the use of dogs as a model for studying ADHD. This condition entails behavioral problems expressed through impulsivity, attention issues, hyperactivity, and/or aggression, compromising the quality of life for both the caregiver and the dog. The pathophysiology of ADHD-like is complex and is associated with dysregulation of various neurotransmitters such as serotonin and dopamine. The expression of ADHD-like behavior in dogs would appear to depend on a classical gene–environment interaction as is the case with many neurological disorders in humans. In addition to the described symptomatology, ADHD-like dogs can exhibit strong comorbidities with compulsive behaviors, aggressiveness, inappropriate elimination and fearfulness, in addition to epilepsy, foreign body ingestion, and pruritus. In spite of the fact that there is no veterinary consensus about the diagnosis of ADHD-like, some validated questionnaires could be helpful, but these cannot be used as a unique diagnostic tool. The use of drugs, such as fluoxetine, in addition to an adequate environmental enrichment, relaxation protocols, and behavior modification can achieve an adequate quality of life for both the dog and caregivers. [ABSTRACT FROM AUTHOR]

Simple Summary: Heartworm disease is a zoonotic illness primarily affecting dogs and cats, which poses a significant risk to public health. Gran Canaria (Canary Island, Spain) serves as a hyperendemic region for this disease, offering a model for its study. This research aimed to track Dirofilariosis prevalence and distribution among dogs, cats, and residents from 1994 to 2020. Data from 5841 dogs, 1203 cats, and 1604 humans were analyzed, considering geographical and climatic factors. Results revealed a decline in Dirofilaria immitis prevalence over the years: from 67.02% in dogs (1994) to 15.81% (2020), and from 33.03% (2010) to 17.20% (2020) in cats. Human incidence dropped from 18.66% (2008) to 8.27% (2020). Prevalence was highest in temperate cold zone (TC) and temperate mild zone (TM) climates. Despite a decrease in prevalence, Gran Canaria remains hyperendemic. The study underscores the significance of a "One Health" approach and highlights ongoing risks of disease transmission. Dirofilariosis is a zoonotic disease that mainly affects dogs and cats, with a high risk to public health. The island of Gran Canaria (Spain) has been shown to be a hyperendemic area of infection and, therefore, a model for studying the evolution of the disease. The objective of this study was to track the prevalence and distribution of heartworm in dogs, cats, and residents of Gran Canaria from 1994 to 2020, using published and unpublished data. Blood samples from 5841 dogs, 1203 cats, and 1604 humans were collected in the years analyzed, considering geographical and isoclimatic factors. In 1994, a prevalence of Dirofilaria immitis of 67.02% in dogs was reported, while in 2020 it was 15.81%. In cats, the seroprevalence in 2010 was 33.03%, compared to 17.20% in 2020. The incidence of D. immitis in humans in 2008 was 18.66%, while in 2020 it was 8.27%. For all study groups, temperate cold zone (TC) and temperate mild zone (TM) climates had the highest prevalence. Throughout these 20 years, the prevalence of heartworm disease has decreased. Despite this, it continues to be a hyperendemic island. This study highlights the importance of using the "One Health" perspective and the risks of contagion of the disease. [ABSTRACT FROM AUTHOR]

Fine-grained few-shot image classification is a popular research area in deep learning. The main goal is to identify subcategories within a broader category using a limited number of samples. The challenge stems from the high intra-class variability and low inter-class variability of fine-grained images, which often hamper classification performance. To overcome this, we propose a fine-grained few-shot image classification algorithm based on bidirectional feature reconstruction. This algorithm introduces a Mixed Residual Attention Block (MRA Block), combining channel attention and window-based self-attention to capture local details in images. Additionally, the Dual Reconstruction Feature Fusion (DRFF) module is designed to enhance the model's adaptability to both inter-class and intra-class variations by integrating features of different scales across layers. Cosine similarity networks are employed for similarity measurement, enabling precise predictions. The experiments demonstrate that the proposed method achieves classification accuracies of 96.99%, 98.53%, and 89.78% on the CUB-200-2011, Stanford Cars, and Stanford Dogs datasets, respectively, confirming the method's efficacy in fine-grained classification tasks. [ABSTRACT FROM AUTHOR]

The interest in the development and therapeutic application of long-acting injectable products for chronic or long-term treatments has experienced exponential growth in recent decades. TV-46000 (Uzedy, Teva) is a long-acting subcutaneous (sc) injectable formulation of risperidone, approved for the treatment of schizophrenia in adults. Following sc injection, the copolymers together with risperidone precipitate to form a sc depot under the skin to deliver therapeutic levels of risperidone over a prolonged period of either 1 month or 2 months, depending upon the dose. This work presents the strategy and the results of the physiologically-based pharmacokinetic (PBPK) modeling and establishing of in vitro–in vivo correlation (IVIVC) for the prediction of TV-46000 pharmacokinetic profile in humans, using in vitro release, intravenous (iv), and sc single-dose pharmacokinetic data in beagle dogs. The resulting simulated TV-46000 PK profile in humans showed that the shape of the predicted risperidone and its active metabolite 9-OH-risperidone PK profiles was different from the observed one, thus suggesting that the TV-46000 release profile was species-dependent and cannot be directly extrapolated from dog to human. In conclusion, while level A IVIVC cannot be claimed, this work combining PBPK and IVIVC modeling represents an interesting alternative approach for complex injectable formulations where classical methods are not applicable. [ABSTRACT FROM AUTHOR]

Simple Summary: Attention-deficit/hyperactivity disorder (ADHD) is a prevalent neurodevelopmental disorder in humans, characterised by inattention, hyperactivity, and impulsivity. Evidence suggests that family dogs can exhibit behaviours similar to symptoms of ADHD seen in humans. While past research mainly used questionnaires to assess ADHD-like behaviours in dogs, we aimed to create a simple behavioural test battery covering key ADHD symptom dimensions: inattention, hyperactivity, and impulsivity. Using our previously validated questionnaire (Dog ADHD and Functionality Rating Scale–DAFRS; 2024) in a study involving 59 dogs, we correlated owner-rated DAFRS scores with behavioural test outcomes to further validate our questionnaire. In a subsample of 38 dogs, we also determined whether there is a difference in how owner- and trainer-rated ADHD scores are linked with the test variables. Our test battery included attention, plush dog, leash, and sit tests, all showing significant correlations with the relevant questionnaire scores in an expected direction. Both owner- and trainer-rated ADHD questionnaire factor scores showed correspondence with the relevant behavioural test variables. Dogs' training status only showed association with the sit test. The test battery proved to be consistent in time; test–retest analyses demonstrated moderate-to-excellent agreement across all tests. Our findings endorse the validity of our questionnaire and behavioural tests, providing valuable tools for assessing inattention, hyperactivity, and impulsivity in dogs. Family dogs exhibit neuropsychological deficits similar to attention-deficit/hyperactivity disorder (ADHD) symptoms in humans. Questionnaire methods have mostly been used to assess ADHD-like behaviours in dogs. In addition to our validated questionnaire (Dog ADHD and Functionality Rating Scale–DAFRS; 2024), we developed a simple behavioural test battery covering the ADHD symptom domains (i.e., inattention, hyperactivity, and impulsivity) in dogs. Our main aim was (i) to provide a final external validation step to the DAFRS by examining its associations with the test variables (N = 59); and (ii) to compare owner- and trainer-rated factor scores' associations with the test variables (n = 38). We developed four tests covering the ADHD symptom domains: the attention test (inattention), the plush dog test (impulsivity), the leash test, and the sit test (hyperactivity). All four behavioural variables correlated with their respective questionnaire scores, i.e., the strongest for hyperactivity, and the least strong for inattention. Both owner- and trainer-rated scores (n = 38) correlated with the relevant test variables in an expected direction. Dogs' training status was linked only to the sit test results. Test–retest analyses (n = 34) indicated moderate-to-excellent agreement across all behavioural variables. Our findings support the validity of our novel human-analogue questionnaire for dogs as the behavioural tests strongly correlate with the relevant questionnaire scores, indicating that the two constructs together can effectively assess inattention, hyperactivity, and impulsivity in dogs. [ABSTRACT FROM AUTHOR]

Unprotected areas with endangered species generally face severe human disturbance. Domestic dogs are a special form of human disturbance and are sympatrically distributed with critically endangered Chinese pangolins in remote mountainous regions of Guangdong, China. Conflicts in habitat utilization between domestic dogs and Chinese pangolins have rarely been evaluated, yet these conflicts might result in a decline in population viability in the wild. To address how domestic dogs affect Chinese pangolins (Manis pentadactyla) in spatiotemporal niches, we used camera traps to obtain information on the distribution and activity of Chinese pangolins and GPS collars to track free-ranging domestic dog activity in the Wuqinzhang and Pengzhai forest areas of Guangdong, China. Combined with environmental variables, we used individual and cave locations to predict a potentially suitable habitat for Chinese pangolins with Maxent. We used the minimum convex polygon method (MCP) to obtain the home ranges of the domestic dogs. Then, we calculated the overlap between the potentially suitable habitat for Chinese pangolins and the home ranges of free-ranging domestic dogs. In the temporal niche, we compared the daily activity rhythms between domestic dogs and Chinese pangolins and assessed the influences of domestic dogs on Chinese pangolins through avoidance–attraction ratios (AARs). Our results show that the potentially suitable habitat of the Chinese pangolin comprises only approximately 24% of the Wuqinzhang forest area and 12% of the Pengzhai forest area. The percentages of habitat overlap were approximately 48% and 71% in the Wuqinzhang and Pengzhai forest areas, respectively. There was less overlap in the temporal niche between Chinese pangolins and free-ranging domestic dogs, but their AAR was significant. Our results reveal that the Chinese pangolin is facing severe disturbances from free-ranging domestic dogs in spatial niches in unprotected areas. We suggest that assessments of Chinese pangolins' survival status should be conducted as soon as possible, especially in unprotected areas. To expand and optimize established nature reserves for the Chinese pangolin, further strengthening of domestic dog management is necessary. [ABSTRACT FROM AUTHOR]

Progressive retinal atrophies (PRAs) are a genetically heterogeneous group of inherited eye diseases that affect over 100 breeds of dog. The initial clinical sign is visual impairment in scotopic conditions, as a consequence of rod photoreceptor cell degeneration. Photopic vision degeneration then follows, due to progression of the disease to the cone photoreceptors, and ultimately results in complete blindness. Two full-sibling English Shepherds were diagnosed with PRA at approximately 5 years old and tested clear of all published PRA genetic variants. This study sought to identify the novel PRA-associated variant segregating in the breed. We utilised a combined approach of whole genome sequencing of the probands and homozygosity mapping of four cases and 22 controls and identified a short interspersed nuclear element within an alternatively spliced exon in FAM161A. The XP_005626197.1 c.17929_ins210 variant was homozygous in six PRA cases and heterozygous or absent in control dogs, consistent with a recessive mode of inheritance. The insertion is predicted to extend exon 4 by 39 aberrant amino acids followed by an early termination stop codon. PRA is intractable to treatment, so the development of a genetic screening test, based on the associated variant, is significant, because it provides dog breeders/owners with a means of reducing the frequency of the disease variant within this breed as well as minimising the risk of breeding puppies that will develop this blinding disease. [ABSTRACT FROM AUTHOR]

Simple Summary: The presence of Bacteroidetes and Fusobacteria phyla are reported to decrease in dogs with chronic enteropathies, likely due to increased oxidative stress in the colon's inflammatory environment. Adherence to the colonic mucosa is considered an essential step for these bacteria to colonize and interact with the host's epithelium and immune system. No studies on dogs have investigated the adhesion of Bacteroides vulgatus and Fusobacterium varium on paraffin-embedded canine colonic mucosa. This study aims to examine the adhesion capacities of these bacterial species to paraffin-embedded colonic mucosa from healthy dogs and investigate their hydrophobicity properties to determine if this factor explains differences in adhesion capability. The results indicated that both canine B. vulgatus and F. varium adhered in high numbers to canine colonic mucosa; however, B. vulgatus had higher hydrophobicity but adhered in lower numbers than F. varium. In conclusion, both bacteria have probiotic potential, but further research is necessary to determine the efficacy and safety of the strains to be used, and other factors influencing attachment beyond hydrophobicity. The relative abundances of Bacteroidetes and Fusobacteria phyla have been reported to be decreased in dogs with chronic enteropathies. In colitis, obligate anaerobes (e.g., Bacteroides and Fusobacterium) are likely to vanish in response to the heightened oxidative stress in the colon's inflammatory environment. The ability to adhere to the colonic mucosa is viewed as an essential step for obligate anaerobic bacteria to colonize and subsequently interact with the host's epithelium and immune system. The reintroduction of a balanced community of obligate anaerobic bacteria using probiotics can restore the microbial function in the intestine. We found no studies on dogs regarding the adhesion properties of Bacteriodes vulgatus and Fusobacterium varium on paraffin-embedded canine colonic mucosa. Thus, the objective of this study is to investigate the adhesion capacities of these two bacterial species to paraffin-embedded colonic mucosa from healthy dogs. Additionally, we investigated their hydrophobicity properties to determine whether differences in adhesion capability can be explained by this factor. The results of our study showed that B. vulgatus adhered significantly lower than F. varium to the canine colonic mucosa (p = 0.002); however, B. vulgatus showed higher hydrophobicity (46.1%) than F. varium (12.6%). In conclusion, both bacteria have potential as probiotics, but further studies will be required to determine the efficacy and safety of the strains to be used, which strains to use, and the reasons other than hydrophobicity for attachment. [ABSTRACT FROM AUTHOR]

Simple Summary: In regard to Sardinia, studies on canine vector-borne diseases are scarce. This study aimed to examine the most common vector-borne diseases affecting domestic dogs in this area and how clinical signs are associated with the disease. The results highlight that 48% of the tested dogs were positive for at least one pathogen, with 34.5% of them having significant clinical symptoms resembling vector-borne disease infections and 65.5% having no signs. These results suggest that preventive measures should be taken to control the spread of vectors and to reduce the infection risk to humans and pets. Canine vector-borne diseases (CVBDs) comprise a group of infectious diseases caused by a wide range of pathogens transmitted by arthropod vectors. Clinical signs commonly involve symptoms such as fever, anorexia, weight loss, blood disorders, hepatosplenomegaly, and others that can lead to death in dogs with comorbidities. Some pathogens responsible for CVBDs constitute a serious threat to human health due to their zoonotic transmission. This study aimed to determine the prevalence of zoonotic vector-borne diseases (Rickettsia rickettsii, Anaplasma phagocytophilum, Ehrlichia canis, Bartonella henselae, and Leishmania infantum) in domestic Sardinian dogs with and without clinical signs of these pathogens. Blood serum samples were collected from 142 dogs and examined through serological analysis. Clinical signs suggestive of these pathogens were also evaluated. The results obtained showed that 33 (33/140; 23.6%), 22 (22/134; 16.4%), 14 (14/142; 9.9%), 20 (20/66; 30.3%), and 26 (26/108; 24.1%) dogs were seropositive for Rickettsia sp., Anaplasma sp., Ehrlichia sp., Bartonella sp., and Leishmania sp. antibodies, respectively. Among these dogs, 12 dogs presented with at least one clinical sign (8.5%), while 18 (12.7%) showed more than two symptoms at the same time. Furthermore, among the asymptomatic dogs (93/142; 65.5%), 13% (n = 12) tested positive for A. phagocytophilum, 12% (n = 11) tested positive for B. henselae, 9% (n = 8) tested positive for E. canis, 12% (n = 11) tested positive for L. infantum, and 19% (n = 18) tested positive for R. rickettsii. This survey represents the first study assessing different canine vector-borne pathogens in dogs from North Sardinia. Since the pathogens detected here represent emerging zoonotic diseases, these results highlight the need to undertake further studies to increase the knowledge of these under-reported vector-borne pathogens in Sardinia. [ABSTRACT FROM AUTHOR]

Simple Summary: This retrospective study aimed to compare clinical and diagnostic features of Trichuris vulpis-infected dogs with those also harboring other intestinal parasites. Forty-five dogs were included, twenty-five solely infected with T. vulpis (categorized as G1) and twenty with concurrent infections (categorized as G2). Weight loss was more frequent in G2 than in G1 (p = 0.006). No significant differences were observed in laboratory abnormalities between G1 and G2. Only diarrhea was more frequent in younger dogs compared to adults (p = 0.007), while adult dogs had higher egg shedding compared to young ones (p = 0.04). A significant positive correlation (r = 0.41; p = 0.005) between egg shedding and age was also found. These findings suggest T. vulpis may cause clinical signs in dogs regardless of age or co-infection. Trichuris vulpis is a parasite of the large intestine of canids and has a global distribution. Despite its well-established epidemiology, the question of its pathogenicity in dogs remains debated. It has been suggested that younger age and concurrent infection with Ancylostoma caninum may be responsible for more severe clinical presentations. This retrospective study aimed to describe the clinical and diagnostic features of T. vulpis-infected dogs and to compare these findings with dogs infected with both T. vulpis and other intestinal parasites (poly-infected dogs). Forty-five dogs were included, with twenty-five being solely infected by T. vulpis and twenty poly-infected dogs. Only weight loss was more frequent (p = 0.006) in poly-infected dogs compared to T. vulpis mono-infected dogs. No significant differences were observed in laboratory abnormalities between mono-infected and poly-infected dogs. Only diarrhea was more frequent (p = 0.007) in younger dogs compared to adults. The egg shedding pattern was significantly higher (p = 0.04) among adult dogs compared to young ones, and there was a significant positive correlation between egg shedding and age (r = 0.41; p = 0.005). These findings suggest that T. vulpis might be responsible for both clinical signs and laboratory abnormalities in dogs, irrespective of the host's age and the presence of other intestinal parasites. [ABSTRACT FROM AUTHOR]

Simple Summary: The microbiome refers to the vast community of microorganisms such as bacteria that inhabit a particular host or ecosystem. Recently, it was discovered that the urine from healthy dogs hosts its own unique microbiome, known as the urobiome. These microbial organisms play important roles in maintaining urinary system health and preventing disease. Although the gut microbiome is heavily influenced by diet, it remains unknown how nutritional features impact the urobiome. Therefore, the purpose of this work was to determine how dietary features alter the urobiome's composition in clinically healthy dogs. Specifically, we examined how nutrient intake (protein, fat, and crude fiber), commercial diet brands, and dietary diversity (i.e., the number of unique food sources consumed each day) altered the abundance and diversity of bacteria present in the canine urobiome. We discovered that both commercial diet brands and dietary diversity were associated with distinct shifts in the composition of the urobiome. This discovery highlights the complex relationships between diet and urinary microbes, and these findings could ultimately lead to novel dietary strategies to promote urobiome health. Nutrition plays an important role in shaping the gut microbiome composition, although the impact of diet on the urinary microbiome (i.e., urobiome) remains unknown. The aim of this pilot study was to discover how nutritional features affect the diversity and composition of the urobiome in dogs. Dietary histories were obtained for 15 clinically healthy adult dogs, including limited nutrient (protein, fat, crude fiber), commercial diet brand, and dietary diversity profiles. The urine samples were collected via cystocentesis, followed by sequencing of the bacterial 16S rRNA gene. The data were analyzed to determine associations between major nutrients and dietary sources with the urobiome's composition. The protein, fat, and crude fiber contents had no statistically significant effect on the alpha or beta diversity. However, the beta diversity values differed (PERMANOVA; p = 0.017, R2 = 0.10) between dogs fed one commercial diet brand compared to dogs consuming any other brand. The beta diversity values also differed (p = 0.019, R2 = 0.10) between dogs consuming more diverse daily diets compared to those consuming less diverse diets (≥3 or <3 unique food sources, respectively). Overall, the results of this pilot study suggest that diet might impact the urobiome and support further exploration of the relationship between diet and the urobiome's composition in dogs. [ABSTRACT FROM AUTHOR]

Heartworm disease caused by Dirofilaria immitis is a vector-borne zoonotic disease responsible for the infection of mainly domestic dogs and cats, or these are those for which the most data are known. Humans are an accidental host where a benign, asymptomatic pulmonary nodule may originate. Dirofilaria immitis also harbours the endosymbiont bacteria of the genus Wolbachia, which play a role in moulting, embryogenesis, inflammatory pathology, and immune response. When Wolbachia sp. is released into the bloodstream, endothelial and pulmonary damage is exacerbated, further encouraging thrombus formation and pulmonary hypertension, facilitating congestive heart failure and death of the animal. Previous studies have shown that parasite excretory/secretory products are able to activate the pro-angiogenic pathway (formation of new vessels) to facilitate parasite survival. The aim of this study was to analyse the role of Wolbachia sp. and its relationship with the cellular processes and the angiogenic pathway in a model of human endothelial cells in vitro. The use of recombinant Wolbachia Surface Protein (rWSP) showed that its stimulation exerted an anti-angiogenic effect by detecting an increase in the production of VEGFR-1/sFlt1 and sEndoglin and did not affect the production of VEGFR-2 and mEndoglin (pro-angiogenic molecules). Furthermore, it did not stimulate cell proliferation or migration, although it did negatively stimulate the formation of pseudocapillaries, slowing down this process. These cellular processes are directly related to the angiogenic pathway so, with these results, we can conclude that Wolbachia sp. is related to the stimulation of the anti-angiogenic pathway, not facilitating the survival of D. immitis in vascular endothelium. [ABSTRACT FROM AUTHOR]

Simple Summary: Cancer of the lymphatic system is a prevalent disease in dogs and cats, with older pets being the most affected group. However, it is crucial to recognize that animals of any age can develop this condition. Intestinal lymphoma, a type of neoplasm, arises when lymphocytes—white blood cells—become cancerous and proliferate uncontrollably. Recent research suggests that the microbiota, the community of bacteria and other microorganisms in the intestines, may play a role in the development of various diseases, including intestinal lymphoma. A healthy microbiota supports the immune system and prevents harmful cellular changes. In contrast, an imbalance in these microorganisms, potentially caused by poor diet, antibiotic use, or illness, can lead to inflammation and increase the risk of cancer. Recent advancements have significantly enhanced our understanding of the crucial role animal microbiomes play in veterinary medicine. Their importance in the complex intestinal environment spans immune modulation, metabolic homeostasis, and the pathogenesis of chronic diseases. Dysbiosis, a microbial imbalance, can lead to a range of diseases affecting both individual organs and the entire organism. Microbial disruption triggers inflammatory responses in the intestinal mucosa and disturbs immune homeostasis, increasing susceptibility to toxins and their metabolites. These dynamics contribute to the development of intestinal lymphoma, necessitating rigorous investigation into the role of microbiota in tumorigenesis. The principles explored in this study extend beyond veterinary medicine to encompass broader human health concerns. There are remarkable parallels between the subtypes of lymphoproliferative disorders in animals and humans, particularly Hodgkin's lymphoma and non-Hodgkin's lymphoma. Understanding the etiology of a cancer of the lymphatic system formation is critical for developing both preventive strategies and therapeutic interventions, with the potential to significantly improve patient outcomes. The aim of this study is to discuss the optimal composition of the microbiome in dogs and cats and the potential alterations in the microbiota during the development of intestinal lesions, particularly intestinal lymphoma. Molecular and cellular analyses are also incorporated to detect inflammatory changes and carcinogenesis. A review of the literature on the connections between the gut microbiome and the development of lymphomas in dogs and cats is presented, along with potential diagnostic approaches for these cancers. [ABSTRACT FROM AUTHOR]

Simple Summary: Canine chronic inflammatory enteropathies and Giardia infection are gastrointestinal diseases characterised by inflammation of the digestive mucosa. This inflammatory process can induce alterations in components of the intestinal structure, such as lipids, as well as in the homeostasis and intestinal environment. Consequently, we have theorised that variations in the composition of faecal fatty acids could exist between different digestive disorders and could help in their differentiation as well as a more personalised dietary treatment. Chronic inflammatory enteropathies (CIEs) are classified based on treatment trials, and new methods are being sought for earlier differentiation and characterization. Giardia infection (GIA) is one of the first differential diagnoses and may be present in CIE-affected dogs. The aim of this study was to evaluate the faecal characteristics and faecal fatty acid profile (short, medium, long, and branched-chain fatty acids) in dogs with food-responsive enteropathy (FRE), immunosuppressant-responsive enteropathy (IRE), and dogs infected with Giardia compared to healthy control (HC) animals as a potential non-invasive indicator of intestinal health that helps in the differentiation of CIEs. The C16:1n-7 percentage (p = 0.0001) and C16:1n-7/C16:0 ratio (p = 0.0001) served to differentiate between HC, FRE, and IRE. IRE dogs presented lower levels of short-chain fatty acids (∑SCFAs) (p = 0.0008) and acetic acid (C2) (p = 0.0007) compared to the other three groups and lower propionic acid (C3) (p = 0.0022) compared to HCs. IRE and GIA presented higher faecal fat content (p = 0.0080) and ratio of iso/anteiso branched-chain fatty acids (BCFAs) compared to HC and FRE. Correlations between some fatty acids and desaturation indices with the canine inflammatory bowel disease activity index and faecal characteristics were observed, suggesting that these compounds could play an important role in the pathogenesis of these diseases. [ABSTRACT FROM AUTHOR]

Simple Summary: Screening puppies carefully for heart murmurs during the first veterinary health visit is essential to disclose hidden congenital heart diseases. However, murmurs in puppies can also be innocent. Ideally, puppies with a possible congenital heart disease should not be sold to a new owner by their breeders. Our study aimed to investigate how easy veterinarians find differentiating innocent from pathologic murmurs, and how they manage puppies with a pathological murmur. We sent a questionnaire to veterinary practices in the Netherlands and Belgium, and analyzed 452 responses. Though 88% of the respondents find detecting a heart murmur easy, only 9% find differentiating innocent from pathologic murmurs in puppies easy. Only 80% of veterinarians recommend immediate referral of puppies with a pathologic heart murmur. Most respondents recognize that normal growth and lack of clinical signs do not rule out congenital heart disease. Though there are several centers with a veterinary cardiology specialist employed in the surveyed countries, only 43% of the respondents recommended the referral of a puppy to a veterinary cardiology specialist for murmur investigation. In conclusion, most veterinarians understand the importance of an echocardiography of puppies with loud murmurs, but they acknowledge the difficulty in distinguishing innocent from pathological murmurs. Background: Heart murmurs in puppies can be innocent or pathologic; the latter is almost always related to a congenital heart disease. Differentiating between these murmurs can be challenging for practicing veterinarians, but this differentiation is essential to ensure the best prognosis for puppies having a congenital heart disease. Our study aimed to reveal how veterinarians manage puppies with a heart murmur. Methods: A web-based questionnaire was sent to Dutch and Belgian veterinary practices. Results: Data from 452 respondents were analyzed. Though 88% of the respondents find detecting a heart murmur easy, only 9% find differentiating innocent murmurs from pathologic murmurs in puppies easy. Of the respondents, only 80% recommend immediate additional examination when detecting a loud heart murmur during the first veterinary health check at 6 weeks of age. Most of the respondents are aware that normal growth and the absence of clinical signs do not exclude severe congenital heart disease. Of the respondents, 31% were uncertain whether early surgical intervention could lead to improved outcomes. Conclusions: Veterinarians are aware of the importance of echocardiography for puppies with a loud heart murmur, and recognize their limitations when differentiating an innocent from a pathological heart murmur in a puppy. [ABSTRACT FROM AUTHOR]

Simple Summary: Changes in renal blood flow may play an important role in the onset and progression of kidney disease. Evaluation of this parameter is of great interest because it may be reduced early in the progression of renal disease even before other indications of renal dysfunction. Non-invasive measurement of renal blood flow would greatly advance our understanding of renal disease and aid in evaluating therapeutic approaches. An imaging method that allows this and offers several advantages over other imaging methods is the magnetic resonance imaging (MRI) method, arterial spin labeling (ASL). However, ASL-MRI has not been previously used for renal perfusion assessment in dogs and parameters required for accurate quantification in this species are unknown. In this study, critical parameters for renal perfusion quantification with ASL-MRI in dogs were determined. The use of dog-specific parameters obtained from this study resulted in lower perfusion values than those obtained by using standard scanner settings. In conclusion, this study determined preliminary parameters essential for ALS-MRI-based renal blood flow quantification in dogs. These optimized parameters could provide a more reliable estimate of renal blood flow for dogs when using ASL-MRI. Further research is needed to confirm these values, but it can help guide future research. Arterial spin labeling (ASL) MRI allows non-invasive quantification of renal blood flow (RBF) and shows great potential for renal assessment. To our knowledge, renal ASL-MRI has not previously been performed in dogs. The aim of this pilot study was to determine parameters essential for ALS-MRI-based quantification of RBF in dogs: T1, blood (longitudinal relaxation time), λ (blood tissue partition coefficient) and TI (inversion time). A Beagle was scanned at 3T with a multi-TI ASL sequence, with TIs ranging from 250 to 2500 ms, to determine the optimal TI value. The T1 of blood for dogs was determined by scanning a blood sample with a 2D IR TSE sequence. The water content of the dog's kidney was determined by analyzing kidney samples from four dogs with a moisture analyzer and was subsequently used to calculate λ. The optimal TI and the measured values for T1,blood, and λ were 2000 ms, 1463 ms and 0.91 mL/g, respectively. These optimized parameters for dogs resulted in lower RBF values than those obtained from inline generated RBF maps. In conclusion, this study determined preliminary parameters essential for ALS-MRI-based RBF quantification in dogs. Further research is needed to confirm these values, but it may help guide future research. [ABSTRACT FROM AUTHOR]

Simple Summary: The proximodistal patellar position in canine pathologies, including patella luxation, cruciate ligament rupture, and osteoarthrosis, is not fully understood, while this topic is much better understood in human medicine. Various methods are used to confirm the proximodistal patellar position, but they all require calculations and are infrequently used in everyday practice. In a two-step study with three observers, we investigated, for the first time in veterinary medicine, the applicability of a new, simple method derived from human medicine called the tibial plateau–patella angle (TPPA). This method is independent of the knee angle and magnification, does not require any calculations, and consists of just two lines. This study started with cadavers at different stifle angles. Subsequently, we used 100 X-rays at the optimal stifle angle based on these findings. In both study phases, our results revealed strong agreement among observers. The TPPA varied with the stifle angle but remained consistent across the different weight groups. We concluded that the TPPA is directly dependent on the stifle angulation and tends to be lower with a higher stifle extension angle. It could be difficult for some observers to establish the exact caudal border of the tibial plateau while measuring the TPPA. While the TPPA method shows promise, further evaluation, including breed-specific and pathological considerations, is necessary. Estimating a dog's patellar position involves various methods, which categorize it as norma, alta (high), or baja (low). However, they require various calculations. We aimed to evaluate the clinical applicability of a new method, the tibial plateau–patella angle (TPPA). This could aid in planning patella luxation surgery, estimating the patella position after TPLO and various osteotomies. We conducted a two-step study: first, on 15 stifles without pathologies from nine canine cadavers, and second, using 100 patient X-rays from the archive. Three stifle angle positions (45 ± 5°, 90 ± 5°, and 135 ± 5°) and three weight groups (S, M, and L) were evaluated in the first part of this study. Based on these results, the second part of this study was conducted using 100 pathology-free radiographs at the optimal stifle angle (90 ± 5°) from the archive. All radiographs were measured by three observers with varying levels of experience. Our results indicate that the stifle angle significantly impacted the TPPA, whereby lower values were detected with higher stifle angles, which remained consistent within the weight groups. High inter- and intra-observer agreement was achieved. The physiological TPPA values ranged from 26.7° to 48.8°, remaining consistent within the various weight groups. Observer 3 in Group S exhibited a 20% (insignificant) deviation, possibly due to challenges in determining the caudal point of the tibial plateau. In contrast with humans, TPPA values in dogs are negatively correlated with stifle angles, independent of weight. Our reliable and reproducible protocol suggests the potential benefits of training on small-breed dogs stifles. [ABSTRACT FROM AUTHOR]

Simple Summary: Nutritional secondary hyperparathyroidism in dogs arises from either a deficiency in vitamin D or an improper calcium-to-phosphorus ratio in their diet. This report covers four cases of large-breed puppies fed exclusively boneless, non-supplemented raw meat diets. These puppies exhibited severe pain and difficulty walking. Imaging studies, including radiographs and computed tomography scans, showed decreased bone density, and two of the puppies suffered pathological fractures, resulting in their euthanasia. The other two puppies recovered quickly after receiving pain relief and a balanced commercial diet. Nutritional secondary hyperparathyroidism is triggered by low levels of active vitamin D and calcium, causing elevated parathyroid hormone levels in the blood to correct mineral imbalances. This mechanism includes pulling calcium from the bones, making the bones weak and increasing fracture risk. Once uncommon due to balanced commercial dog foods, nutritional secondary hyperparathyroidism has reappeared with the trend of feeding raw meat diets. Timely diagnosis and treatment are vital for recovery, highlighting the necessity of balanced diets to prevent serious skeletal and neurological problems in developing puppies. Nutritional secondary hyperparathyroidism (NSH) in dogs is a condition that develops in response to a vitamin D deficiency or an imbalanced calcium-to-phosphorus ratio in dog food. Puppies of large-breed dogs exclusively fed a non-supplemented, boneless raw meat diet are especially susceptible to developing NSH due to their elevated calcium requirement. Reports on NSH in companion animals have been sparse in the last decades due to dog owners having easy access to commercially balanced dog foods. However, with the rising popularity of meat-based raw feeding, this condition has re-emerged. In this case series, four large-breed puppies fed exclusively non-supplemented, boneless raw meat diets presented with complaints of acute onset of pain and paresis. Radiographs and/or computed tomography (CT) scans showed reduced radio density of the skeleton in all four puppies. Two of the dogs had pathological fractures, and these two puppies were euthanized. One was subjected to a post mortem examination, which revealed cortical bone resorption and hypertrophy of the parathyroid glands. The remaining two puppies rapidly improved after receiving pain medication and a commercial, balanced diet. This case series demonstrates a risk of young dogs developing severe neurological deficits when fed a non-supplemented, boneless raw meat diet. [ABSTRACT FROM AUTHOR]

Simple Summary: Septic exudates in the body cavities of dogs and cats are considered a critical clinical condition. The current elective diagnostic tools for detecting septic effusion are bacterial culture and fluid cytology. Although culture is considered the gold standard, clinicians may not have access to the results for several days. This may result in a delayed diagnosis of septic effusion, which may have adverse effects on patient outcomes. This study compared the performances of cytology and bacterial culture in the identification of septic exudative body cavity effusions in dogs and cats. The results of our investigation indicated moderate agreement between cytology and microbiology. Cytology and bacterial culture results for exudative body cavity effusions in dogs and cats can be misleading when conducted individually. To improve diagnostic accuracy, these two methodologies should be integrated. The elective test for the determination of the effusions etiopathogenesis is represented by physico-chemical analysis and cytology. Nevertheless, the bacterial culture and antibiotic sensitivity tests are crucial for setting therapy and for the outcome. This study compared cytology with microbiology in the etiologic diagnosis of exudative body cavity effusions in dogs and cats collected from October 2018 to October 2022. All samples underwent aerobic and anaerobic culture and cytology examination. Bacterial identifications were confirmed using matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry, whereas cytological samples were blindly evaluated either in May Grunwald-Giemsa (MGG) or Gram-stained samples by two board-certified clinical pathologists. A moderate agreement (κ = 0.454) between cytology and bacterial culture was revealed. The sensitivity of the cytological evaluation in our study ranged from 38.5% to 67.9%, and the specificity ranged from 88.9% to 100%, depending on the type of the effusion, so cytology may not be representative of the etiopathogenesis, whereas bacterial culture can misidentify or fail to isolate the correct pathogen for difficult in vitro growing due to the presence of inhibitory substances or contamination. Cytology and bacterial culture results for exudative body cavity effusions in dogs and cats can be misleading if conducted separately, so these two tests should be performed together to increase diagnostic accuracy. [ABSTRACT FROM AUTHOR]