1. [In vivo determination of alkaline phosphatase activity of the intestine of the rainbow trout (Salmo gairdneri R.)].
- Author
-
Bussière D, Bogé G, and Pérès G
- Subjects
- Animals, Glucose metabolism, Glucosephosphates metabolism, Hydrogen-Ion Concentration, Intestinal Absorption drug effects, Kinetics, Microvilli enzymology, Nitrophenols metabolism, Organophosphorus Compounds metabolism, Phlorhizin pharmacology, Alkaline Phosphatase metabolism, Intestines enzymology, Salmonidae metabolism, Trout metabolism
- Abstract
This work has concerned the description of a method suitable for the measurement, on living trout, of the intestinal alkaline phosphatase activity. The experiments consist in the perfusion, in the lumen, of an appropriate substrate and in the prevention of its absorption or that of the products of its hydrolysis. This condition has not been satisfied by using paranitrophenyl phosphate which has been replaced by glucose-1-phosphate. The absorption of glucose produced has been inhibited by phloridzin (10(-4) M) in a KCl (150 mM) solution. Under these conditions an hydrolysis of glucose phosphate has been shown at alkaline pH, which remains linear with time up to 60 min. A research of the Km of the enzyme activity has been carried out.
- Published
- 1987