Introduction: The main cause of anemia in the body is iron deficiency. Iron supplementation is one of the effective methods for reducing iron deficiency and iron-deficiency anemia in women. Accordingly, the present study aimed to determine the challenges of national iron supplementation in female students from the perspective of stakeholders. Methods: This is a qualitative study of content analysis that was conducted in Gonabad city in 2017. Data were analyzed by semi-deep interviews and focus group discussion method. Participants in the present study included high school girl students, parents, school principals, and school health educators. The sampling in the present study was targeted and collected data continued until data saturation. Data management was done with NVivo software, version 11, but data analysis and interpretation were done manually. Also, data were analyzed using the five steps of Graneheim and Lundman. Results: Based on the stakeholder's perspectives, 12 themes of perceived executive or management benefits, perceived individual benefits, physical disadvantages, tablets disadvantages, disadvantages of the program, disadvantages of programming, barriers of pill consumption, educational and information barriers, management and administrative barriers, reform the shape of pills, individual perception modification, and modifying the executive program were effective in the national iron supplementation. Conclusions: Based on the results for implementation a more effective Iron supplement program, it is necessary to design and implement appropriate interventions at individual and interpersonal levels. Also, at the level of inter-organizational and intraorganizational, it is necessary to increase coordination and cooperation. [ABSTRACT FROM AUTHOR]