1. FBXW7Mutations Promote Cell Proliferation, Migration, and Invasion in Cervical Cancer
- Author
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Yang Zou, Yang Bicheng, Jiang-Yan Zhou, Ouping Huang, Mei-rong Liang, Feng Wang, Ziyu Zhang, Yong Luo, and Fa-Ying Liu
- Subjects
0301 basic medicine ,Cervical cancer ,Mutation ,Cell growth ,Protein subunit ,Cell ,General Medicine ,Biology ,medicine.disease_cause ,medicine.disease ,Ubiquitin ligase ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Germline mutation ,030220 oncology & carcinogenesis ,medicine ,biology.protein ,Cancer research ,Gene ,Genetics (clinical) - Abstract
Aim: Cervical cancer is the most common gynecological cancer. Recent studies have revealed that the F-box and WD repeat domain containing 7 (FBXW7) gene, which encodes a subunit of Skp1-Cul1-F-box protein (SCF) ubiquitin ligase, is frequently mutated in cervical squamous cell carcinomas. In this study, we investigated whether Chinese cervical cancer cells also harbor these mutations. Methods: Using PCR and sequencing assays, a total of 190 specimens from Han Chinese patients with cervical cancer were analyzed for FBXW7 mutations. Results: Two FBXW7 mutations (p.R479P and p.L443H), were identified from a study of 145 (1.4%) cervical squamous cell carcinomas. The p.L443H somatic mutation has not been previously reported. Functional assays showed that both of these FBXW7 mutations could promote cell proliferation, migration, and invasion. Conclusion: A low frequency (1.4%) of cervical squamous cell carcinomas were identified with FBXW7 mutations. We did, however, identify a novel FBXW7 mutation. Our results also demonstrated that the identified FBXW7 mutations could promote cell proliferation, migration, and invasion in cervical cancer cells.
- Published
- 2019