1. Anti-Inflammatory Effect of Heme Oxygenase-1 Toward Porphyromonas gingivalis Lipopolysaccharide in Macrophages Exposed to Gomisins A, G, and J
- Author
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Sang-Joon Lee, Rajaseker Seetharaman, Young-Whan Choi, Young Hun Kim, Jum Soon Kang, Sunyoung Park, Da Jung Park, Sun Gun Kim, and Eun Yeon Ryu
- Subjects
Lipopolysaccharides ,Lipopolysaccharide ,medicine.medical_treatment ,Interleukin-1beta ,Anti-Inflammatory Agents ,Medicine (miscellaneous) ,Inflammation ,Dioxoles ,Biology ,Lignans ,Cell Line ,Proinflammatory cytokine ,Microbiology ,Cyclooctanes ,Mice ,Gingivitis ,chemistry.chemical_compound ,medicine ,Animals ,Polycyclic Compounds ,Porphyromonas gingivalis ,Schisandra ,Nutrition and Dietetics ,Interleukin-6 ,Plant Extracts ,Tumor Necrosis Factor-alpha ,NF-kappa B ,Membrane Proteins ,biology.organism_classification ,Heme oxygenase ,Cytokine ,chemistry ,Fruit ,Tumor necrosis factor alpha ,medicine.symptom ,Full Communications ,Heme Oxygenase-1 ,Signal Transduction - Abstract
Periodontitis, a chronic inflammatory periodontal disease that develops from gingivitis, is caused by periodontal pathogenic bacteria such as Porphyromonas gingivalis. Recent studies have focused on the antioxidant, anti–human immunodeficiency virus, anticarcinogenic, and anti-inflammatory properties of gomisins. However, the anti-inflammatory activities of gomisin plants through heme oxygenase-1 (HO-1) signals remain poorly defined. We found that gomisins' anti-inflammatory activity occurs via the induction of HO-1 expression. Gomisins G and J inhibit the production of the pro-inflammatory cytokines tumor necrosis factor-α, interleukin-1β, and interleukin-6 and also block nuclear factor-κB activation in Raw264.7 cells stimulated with P. gingivalis lipopolysaccharide. Furthermore, pro-inflammatory cytokine production is inhibited through the induction of HO-1 expression. HO-1 expression is induced by all gomisins, but their anti-inflammatory activity via HO-1 signaling is observed with gomisins G and J, and not A. We found that gomisins G and J extracted from Schisandria chinensis can inhibit the P. gingivalis lipopolysaccharide induced-inflammatory responses in Raw264.7 cells.
- Published
- 2011