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Aim: The purpose of the present study was to evaluate the short-term storage of meta-genomic DNA from native oral biofilms on FTA® paper. Materials and Methods: Thirteen volunteers of both sexes received an acrylic splint for intraoral biofilm formation over a period of 48 hours. The biofilms were collected, resuspended in phosphate-buffered saline, and either stored on FTA® paper or directly processed by standard laboratory DNA extraction. The nucleic acid extraction efficiencies were evaluated by 16S rDNA targeted SSCP fingerprinting. The acquired banding pattern of FTA-derived meta-genomic DNA was compared to a standard DNA preparation protocol. Sensitivity and positive predictive values were calculated. Results: The volunteers showed inter-individual differences in their bacterial species composition. A total of 200 bands were found for both methods and 85% of the banding patterns were equal, representing a sensitivity of 0.941 and a false-negative predictive value of 0.059. Conclusion: Meta-genomic DNA sampling, extraction, and adhesion using FTA® paper is a reliable method for storage of microbial DNA for a short period of time. [ABSTRACT FROM AUTHOR]

TEX file as is needed for processing, selecting the file designation of TeX/LaTeX Suppl File. Graph Professor Waterman is one of the founders of the field of computational biology and has made many fundamental contributions to the field. [Extracted from the article]

Planetary health omics has been featured in many articles in I OMICS i over the past several years, and builds upon the rise of planetary health (Horton et al., [4]), the lessons learned from the COVID-19 pandemic, and the introduction of digital technologies to systems science. Planetary health omics is a new field that expands the boundaries and tools of its predecessor fields public health genomics and global health. Planetary health omics as a new field of inquiry in systems science is relevant and timely going forward with COVID-19 as an endemic disease (Katzourakis, [5]), and for both communicable and noncommunicable chronic diseases. [Extracted from the article]

ABSTRACTThe effect of stem bark powder from paper mulberry (PMSB) on serum glucose, insulin, fructosamine, and lipid concentrations, as well as enzyme activities that serve as liver injury markers, was investigated in genetically diabetic Otsuka Long-Evans Tokushima fatty (OLETF) rats. Both nondiabetic Long-Evans Tokushima Otsuka (LETO) rats and diabetic OLETF rats (30 weeks old) were fed a semisynthetic diet with or without 50 g/kg PMSB for 8 weeks and then compared. The OLETF control rats showed a high amount of daily water intake in comparison to those in the LETO group. The concentrations of glucose, fructosamine, total lipid, triglyceride, total cholesterol, and high-density lipoprotein-cholesterol and the activities of aspartate aminotransferase and alanine aminotransferase (ALT) in serum were higher in the OLETF control rats than those in the LETO control rats. However, PMSB ingestion decreased the serum levels of glucose, fructosamine, triglyceride, and total cholesterol and the activity of ALT in the OLETF rats, but not in the LETO rats. The concentration of serum insulin was also significantly increased by PMSB consumption in the OLETF rats compared to the OLETF control rats. These results suggest that PMSB might have an antihyperglycemic effect in the OLETF rat and that the increased blood insulin level would be an important regulatory factor for improving hyperglycemia in the current animal model. [ABSTRACT FROM AUTHOR]

I Cellular Reprogramming i is the only peer-reviewed journal dedicated to cellular reprogramming mechanisms, technologies, and applications. The special issue will cover a broad range of topics linked to cellular reprogramming including, but are not limited to: Somatic cell nuclear transfer Cloning and cell fusion Induced pluripotent stem cells Embryonic stem cells and pluripotency Cellular reprogramming biology and technologies Cell and gene therapy based on cell reprogramming paradigms Disease modeling and drug screening utilizing stem cells B Deadline for article submission: April 30, 2023 b Please upload your submission under the manuscript category entitled "25th Anniversary Special Issue (2023).". [Extracted from the article]

In addition to the genetic determinants, inflammation can also be triggered by multiple nongenetic factors including microbiome, toxic compounds, diet, and stress, which affects the epigenome and secretome of tissue microenvironment. Decades of research have shown how the clinical manifestation of inflammation can also be pathological and prime the tissue niche for the progression of myriad complex diseases. [Extracted from the article]

Innovative applications of digital technologies that help decipher the systems biology of SARS-CoV-2, for example, the IoMT, artificial intelligence and machine learning to strengthen phenomics, discovery science, and translational medicine. More than 2 years into the COVID-19 pandemic, the global struggle with SARS-CoV-2 continues. [Extracted from the article]

Graph: hum.2020.29106.cfp6 figure1.jpg CRISPR-based genome editing has revolutionized experimental biology and is being harnessed for human gene therapy. While initial applications of the CRISPR-Cas system were primarily based on the Cas9 endonuclease function, protein engineering has enabled a variety of other DNA- and RNA-altering functions to be guided by the Cas-sgRNA mechanism. I Human Gene Therapy i is now seeking original papers and review articles that describe the design, optimization, and application of these next-generation CRISPR approaches, including, but not limited to, the following technologies: Base editing Epigenome editing CRISPR-transactivation RNA editing Prime editing Anti-CRISPR proteins Novel Cas-based fusion proteins that confer novel functions, sequence-targeted by Cas-sgRNA Demonstrations of the utility of one or more of such technologies in animal models of specific human diseases, including monogenic disorders, is of particular interest. [Extracted from the article]

Graph: hum.2020.29106 figure1.jpg CRISPR-based genome editing has revolutionized experimental biology and is being harnessed for human gene therapy. While initial applications of the CRISPR-Cas system were primarily based on the Cas9 endonuclease function, protein engineering has enabled a variety of other DNA- and RNA-altering functions to be guided by the Cas-sgRNA mechanism. I Human Gene Therapy i is now seeking original papers and review articles that describe the design, optimization, and application of these next-generation CRISPR approaches, including, but not limited to, the following technologies: Base editing Epigenome editing CRISPR-transactivation RNA editing Prime editing Anti-CRISPR proteins Novel Cas-based fusion proteins that confer novel functions, sequence-targeted by Cas-sgRNA Demonstrations of the utility of one or more of such technologies in animal models of specific human diseases, including monogenic disorders, is of particular interest. [Extracted from the article]

Graph: hum.2020.29106.cfp4 figure1.jpg CRISPR-based genome editing has revolutionized experimental biology and is being harnessed for human gene therapy. While initial applications of the CRISPR-Cas system were primarily based on the Cas9 endonuclease function, protein engineering has enabled a variety of other DNA- and RNA-altering functions to be guided by the Cas-sgRNA mechanism. I Human Gene Therapy i is now seeking original papers and review articles that describe the design, optimization, and application of these next-generation CRISPR approaches, including, but not limited to, the following technologies: Base editing Epigenome editing CRISPR-transactivation RNA editing Prime editing Anti-CRISPR proteins Novel Cas-based fusion proteins that confer novel functions, sequence-targeted by Cas-sgRNA Demonstrations of the utility of one or more of such technologies in animal models of specific human diseases, including monogenic disorders, is of particular interest. [Extracted from the article]

Graph CRISPR-based genome editing has revolutionized experimental biology and is being harnessed for human gene therapy. While initial applications of the CRISPR-Cas system were primarily based on the Cas9 endonuclease function, protein engineering has enabled a variety of other DNA- and RNA-altering functions to be guided by the Cas-sgRNA mechanism. I Human Gene Therapy i is now seeking original papers and review articles that describe the design, optimization, and application of these next-generation CRISPR approaches, including, but not limited to, the following technologies: Base editing Epigenome editing CRISPR-transactivation RNA editing Prime editing Anti-CRISPR proteins Novel Cas-based fusion proteins that confer novel functions, sequence-targeted by Cas-sgRNA Demonstrations of the utility of one or more of such technologies in animal models of specific human diseases, including monogenic disorders, is of particular interest. [Extracted from the article]