1. Human parainfluenza virus 3 neuraminidase activity contributes to dendritic cell maturation.
- Author
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Horga MA, Macip S, Tuyama AC, Tan MC, and Gusella GL
- Subjects
- Cell Differentiation, Chemokines biosynthesis, Clostridium perfringens enzymology, Clostridium perfringens immunology, Cytokines biosynthesis, Genes, Viral, Humans, In Vitro Techniques, Mutation, Neuraminidase genetics, Parainfluenza Virus 3, Human genetics, Parainfluenza Virus 3, Human pathogenicity, Respirovirus Infections immunology, Respirovirus Infections pathology, Species Specificity, Dendritic Cells immunology, Dendritic Cells pathology, Neuraminidase immunology, Parainfluenza Virus 3, Human enzymology, Parainfluenza Virus 3, Human immunology
- Abstract
Mechanisms of dendritic cells (DCs) immunomodulation by parainfluenza viruses have not been characterized. We analyzed whether the human parainfluenza 3 (HPF3) virus hemagglutinin-neuraminidase glycoprotein (HN) might influence DC maturation. HN possesses a receptor binding function and a neuraminidase or desialidating activity. To assess whether the neuraminidase activity of HN affects DC maturation, human myeloid DCs were exposed to either live or UV-inactivated HPF3 viruses containing wild type or a mutated form of HN with decreased neuraminidase activity. Exposure of human DCs to either UV-inactivated or live virus induced up-regulation of CD83 and CD86 surface markers, morphological changes, and a cytokine expression pattern consistent with maturation. However, the level of maturation was found to be lower in DCs infected with the neuraminidase deficient variant as compared to the wild type. These results suggest that during the course of viral infection, HN's neuraminidase activity may play an important role contributing to maturation and activation of DCs.
- Published
- 2005
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