1. Extracellular Matrix Degradation Products Downregulate Neoplastic Esophageal Cell Phenotype.
- Author
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Saldin LT, Patel S, Zhang L, Huleihel L, Hussey GS, Nascari DG, Quijano LM, Li X, Raghu D, Bajwa AK, Smith NG, Chung CC, Omstead AN, Kosovec JE, Jobe BA, Turner NJ, Zaidi AH, and Badylak SF
- Subjects
- Animals, Apoptosis, Autophagy, Cell Cycle, Cell Line, Tumor, Cell Proliferation, Cell Shape, DNA Replication, Esophageal Neoplasms genetics, Esophageal Neoplasms metabolism, Gene Expression Regulation, Neoplastic, Humans, Phenotype, Phosphatidylinositol 3-Kinases metabolism, Phosphorylation, Proto-Oncogene Proteins c-akt metabolism, Signal Transduction, Swine, Urinary Bladder metabolism, Down-Regulation, Esophageal Neoplasms pathology, Extracellular Matrix metabolism
- Abstract
Impact Statement: Extracellular matrix (ECM) biomaterials were used to treat esophageal cancer patients after cancer resection and promoted regrowth of normal mucosa without recurrence of cancer. The present study investigates the mechanisms by which these materials were successful to prevent the cancerous phenotype. ECM downregulated neoplastic esophageal cell function (proliferation, metabolism), but normal esophageal epithelial cells were unaffected in vitro , and suggests a molecular basis (downregulation of PI3K-Akt, cell cycle) for the promising clinical results. The therapeutic effect appeared to be enhanced using homologous esophageal ECM. This study suggests that ECM can be further investigated to treat cancer patients after resection or in combination with targeted therapy.
- Published
- 2019
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