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Start Over Author "R. Serino" Publisher lippincott williams & wilkins
3 results on '"R. Serino"'

1. Centrally administered orexin/hypocretin activates HPA axis in rats.

Author

Kuru M, Ueta Y, Serino R, Nakazato M, Yamamoto Y, Shibuya I, and Yamashita H

Subjects
Adrenocorticotropic Hormone blood, Animals, Carrier Proteins pharmacology, Corticosterone blood, Gene Expression drug effects, Genes, fos, Histocytochemistry, In Situ Hybridization, Injections, Intraventricular, Male, Neuropeptides pharmacology, Orexins, Osmolar Concentration, Paraventricular Hypothalamic Nucleus metabolism, RNA, Messenger metabolism, Rats, Rats, Wistar, Carrier Proteins administration & dosage, Hypothalamo-Hypophyseal System drug effects, Intracellular Signaling Peptides and Proteins, Neuropeptides administration & dosage, Pituitary-Adrenal System drug effects
Abstract

The effects of i.c.v. administration of orexin/hypocretin on plasma ACTH, corticosterone and c-fos mRNA in the paraventricular nucleus (PVN) of the rat were examined. Plasma ACTH levels were markedly increased at 30 min after i.c.v. administration of orexin-A. Plasma corticosterone levels were significantly increased in a dose-related manner 30 min after i.c.v. administration of orexin-A and orexin-B. In situ hybridization histochemistry revealed that the induction of the c-fos mRNA in the parvocellular division of the PVN was increased in a dose-related manner 30 min after i.c.v. administration of orexin-A and orexin-B. These results suggest that central orexin/hypocretin activates hypothalamo-pituitary-adrenal (HPA) axis and may be involved in stress-induced activation of the HPA axis.

Published
2000
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2. Upregulation of neuronal NOS mRNA in the PVN and SON of inherited diabetes insipidus rats.

Author

Yamamoto Y, Ueta Y, Nomura M, Serino R, Kabashima N, Shibuya I, and Yamashita H

Subjects
Animals, Diabetes Insipidus genetics, Gene Expression, Histocytochemistry, In Situ Hybridization, Rats, Rats, Brattleboro, Rats, Wistar, Up-Regulation, Diabetes Insipidus metabolism, Neurons metabolism, Nitric Oxide Synthase genetics, Paraventricular Hypothalamic Nucleus metabolism, RNA, Messenger metabolism, Supraoptic Nucleus metabolism
Abstract

We investigated the expression of neuronal nitric oxide synthase (nNOS) gene in the paraventricular (PVN) and supraoptic nuclei (SON) of rats with inherited diabetes insipidus (DI), using in situ hybridization histochemistry. The DI rats showed hypo-osmotic polyuria and polydipsia with arginine vasopressin (AVP) deficiency. The expression of nNOS gene in the PVN and SON in homozygous (di/di) rats was significantly increased in comparison to normal Wistar and heterozygous (di/+) rats. nNOS gene-expressing cells were distributed throughout the PVN and SON, including the divisions of AVP and oxytocin gene expressing cells in di/di rats. These results suggest that the expression of nNOS gene is upregulated in the magnocellular neurons in the PVN and SON of inherited DI rats.

Published
1997
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3. PACAP type I receptor gene expression in the paraventricular and supraoptic nuclei of rats.

Author

Nomura M, Ueta Y, Serino R, Kabashima N, Shibuya I, and Yamashita H

Subjects
Animals, Arginine Vasopressin biosynthesis, Arginine Vasopressin genetics, Corticotropin-Releasing Hormone biosynthesis, Corticotropin-Releasing Hormone genetics, Gene Expression, Histocytochemistry, In Situ Hybridization, Male, Neuropeptides metabolism, Neuropeptides physiology, Neurotransmitter Agents metabolism, Neurotransmitter Agents physiology, Oxytocin biosynthesis, Oxytocin genetics, Pituitary Adenylate Cyclase-Activating Polypeptide, Rats, Rats, Wistar, Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I, Receptors, Pituitary Hormone genetics, Sulfur Radioisotopes, Paraventricular Hypothalamic Nucleus metabolism, Receptors, Pituitary Hormone biosynthesis, Supraoptic Nucleus metabolism
Abstract

We have examined the distribution of pituitary adenylate cyclase-activating polypeptide (PACAP) type I receptor gene expression in the rat paraventricular (PVN) and supraoptic nuclei (SON) using in situ hybridization histochemistry. PACAP type I receptor gene was expressed moderately in the whole area of the PVN and the SON. In particular, PACAP type I gene transcripts were found in both the magnocellular and the parvocellular parts of the PVN. The results are consistent with the hypothesis that neurosecretory cells in the PVN and the SON might be modulated by PACAP through PACAP type I receptor.

Published
1996
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