Objective: To compare physician practices, attitudes, and barriers toward human papillomavirus (HPV) vaccination., Materials and Methods: A Web-based survey that assessed the practice of HPV vaccination, perceived barriers, and opinions of potential vaccine effects was distributed to obstetrician/gynecologists, internists, and pediatricians within a single health care system. A total of 1,488 physicians located in tertiary, community, and private settings were sent the survey. Data were analyzed using multivariate logistic regression., Results: A total of 424 (28.9%) of 1,488 physicians participated. Of these physicians, 80% offer the vaccine. Female, primary care, and community and private practice physicians were associated with increased vaccination, compared with male, subspecialty, and tertiary care physicians, respectively. Physicians who reported no barriers to vaccination were more likely to offer the vaccine (odds ratio = 1.74, 95% CI = 1.04-2.93, p =.04). The greatest barrier to vaccination was reimbursement concerns., Conclusions: Most physicians offer the HPV vaccine. Addressing barriers to vaccination and resolving practical concerns may increase HPV vaccination.