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Abstract: Purpose: Ingestion of whey protein increases myofibrillar but not muscle connective protein synthesis rates. Recently, we defined a whey and collagen protein blend (5:1-ratio) to optimize post-prandial plasma amino acid availability. Here, we assessed the ability of this blend to increase myofibrillar and muscle connective protein synthesis rates at rest and during early recovery from exercise.Methods: In a randomized, double-blind, parallel design, 28 men (age: 25 ± 5 y; BMI: 23.6 ± 2.3 kg/m2) were randomly allocated to ingest either 30 g of protein (25 g whey/5 g collagen; BLEND, n = 14) or a non-caloric placebo (PLA, n = 14) following a single session of unilateral leg resistance-type exercise. Participants received primed continuous L-[ring-13C6]-phenylalanine infusions with blood and muscle biopsy samples collection for 5 hours post-prandially to assess myofibrillar and muscle connective protein synthesis rates.Results: Protein ingestion strongly increased plasma amino acid concentrations, including plasma leucine and glycine concentrations (P < 0.001), with no changes following placebo ingestion (P > 0.05). Post-prandial myofibrillar and muscle connective protein synthesis rates were higher in the exercised compared to the rested leg (P < 0.001). In addition, myofibrillar protein synthesis rates were higher in BLEND compared to PLA in both the rested (0.038 ± 0.008 and 0.031 ± 0.006%·h-1, respectively; P < 0.05) and exercised (0.052 ± 0.011 and 0.039 ± 0.009%·h-1, respectively; P < 0.01) leg. Muscle connective protein synthesis rates were higher in BLEND compared to PLA in the rested (0.062 ± 0.013 and 0.051 ± 0.010%·h-1, respectively; P < 0.05), but not the exercised (0.090 ± 0.021 and 0.079 ± 0.016%·h-1, respectively; P = 0.11) leg.Conclusions: Ingestion of a whey (25 g) plus collagen (5 g) protein blend increases both myofibrillar and muscle connective protein synthesis rates at rest and further increases myofibrillar but not muscle connective protein synthesis rates during recovery from exercise in recreationally active, young men., Competing Interests: Conflict of Interest and Funding Source: This study was funded by a public-private cooperation between Maastricht University, The Netherlands, GELITA AG, Germany, and TKI Health~Holland, The Netherlands. L. J. C. vL. and his laboratory has received research grants, consulting fees, speaking honoraria, or a combination of these for research on the impact of exercise and nutrition on muscle metabolism, which include research funding from companies that produce collagen such as GELITA AG and PB Leiner. A full overview on research funding is provided at https://www.maastrichtuniversity.nl/l.vanloon. U. B. is an employee of GELITA AG. K. Baar has received has also received grants, consulting fees, speaking honoraria, and donations from nutritional companies such as PepsiCo, Bergstrom Nutrition, Ynsect, and GelTor to study the effect of dietary collagen on endogenous collagen synthesis. T. S. was supported by a research grant from GELITA AG to perform studies on collagen protein ingestion and post-exercise recovery. T. A., J. K., W. J. H. H., F. K. H., A. M. H., J. M. S., J. M. X. vK. and J. P. G. G. report no conflicts of interest related to this work. The results of the study are presented clearly, honestly, and without fabrication, falsification, or inappropriate data manipulation. The results of the present study do not constitute endorsement by the American College of Sports Medicine., (Copyright © 2024 by the American College of Sports Medicine.)

Introduction: Plant-derived proteins have received considerable attention as an alternative to animal-based proteins and are now frequently used in both plant-based diets and sports nutrition products. However, little information is available on the anabolic properties of potato-derived protein. This study compares muscle protein synthesis rates after the ingestion of 30 g potato protein versus 30 g milk protein at rest and during recovery from a single bout of resistance exercise in healthy, young males., Methods: In a randomized, double-blind, parallel-group design, 24 healthy young males (24 ± 4 yr) received primed continuous l -[ ring - 13 C 6 ]-phenylalanine infusions while ingesting 30 g potato-derived protein or 30 g milk protein after a single bout of unilateral resistance exercise. Blood and muscle biopsies were collected for 5 h after protein ingestion to assess postprandial plasma amino acid profiles and mixed muscle protein synthesis rates at rest and during recovery from exercise., Results: Ingestion of both potato and milk protein increased mixed muscle protein synthesis rates when compared with basal postabsorptive values (from 0.020% ± 0.011% to 0.053% ± 0.017%·h -1 and from 0.021% ± 0.014% to 0.050% ± 0.012%·h -1 , respectively; P < 0.001), with no differences between treatments ( P = 0.54). In the exercised leg, mixed muscle protein synthesis rates increased to 0.069% ± 0.019% and 0.064% ± 0.015%·h -1 after ingesting potato and milk protein, respectively ( P < 0.001), with no differences between treatments ( P = 0.52). The muscle protein synthetic response was greater in the exercised compared with the resting leg ( P < 0.05)., Conclusions: Ingestion of 30 g potato protein concentrate increases muscle protein synthesis rates at rest and during recovery from exercise in healthy, young males. Muscle protein synthesis rates after the ingestion of 30 g potato protein do not differ from rates observed after ingesting an equivalent amount of milk protein., (Copyright © 2022 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American College of Sports Medicine.)