1. Treadmill exercise with bone marrow stromal cells transplantation potentiates recovery of locomotor function after spinal cord injury in rats
- Author
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Tae-Beom Seo, Chang-Ju Kim, Eun-Sang Ji, and You-Mi Kim
- Subjects
medicine.medical_specialty ,Stromal cell ,Cord ,Physical Therapy, Sports Therapy and Rehabilitation ,Spinal cord injury ,Locomotor function ,Brain-derived neurotrophic factor ,Lesion ,03 medical and health sciences ,0302 clinical medicine ,Neurotrophic factors ,Internal medicine ,medicine ,Orthopedics and Sports Medicine ,Synapsin-I ,business.industry ,Bone marrow stromal cells ,030229 sport sciences ,Anatomy ,medicine.disease ,Transplantation ,medicine.anatomical_structure ,Endocrinology ,nervous system ,Treadmill exercise ,Original Article ,Bone marrow ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
Transplantation of bone marrow stromal cells (BMSCs) is regarded as a promising candidate for the spinal cord injury (SCI). In the present study, we investigated whether treadmill exercise potentiate the effect of BM-SCs transplantation on the functional recovery in the SCI rats. The spi-nal cord contusion injury applied at the T9–T10 level using the impactor. Cultured BMSCs were transplanted into the lesion at 1 week after SCI induction. Treadmill exercise was conducted for 6 weeks. Basso-Beat-tie-Bresnahan (BBB) scale for locomotor function was determined. Sprouting axons in the lesion cavity were detected by immunofluores-cence staining for neurofilament-200. Brain-derived neurotrophic factor (BDNF) and synapsin-I expressions were analyzed using western blot-ting. BMSCs transplantation improved BBB score and increased ex-pressions of neurofilament-200, BDNF, and synapsin-I in the SCI rats. Treadmill exercise potentiated the improving effect of BMSCs trans-plantation on BBB score in the SCI rats. This potentiating effect of treadmill exercise could be ascribed to the enhancement of BDNF ex-pression in the SCI rats.
- Published
- 2017