1. Prominent IL-12 Production and Tumor Reduction in Athymic Nude Mice after Toxoplasma gondii Lysate Antigen Treatment
- Author
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Kyoung Ho Pyo, You Won Lee, Eun Hee Shin, Bong Kwang Jung, Jong-Yil Chai, and Chun Feng Xin
- Subjects
Cellular immunity ,anti-tumorigenesis ,Angiogenesis ,athymic mouse ,Athymic mouse ,Mice, Nude ,Toxoplasma gondii ,Antigens, Protozoan ,NF-κB ,TIMP-1 ,Antigen ,Neoplasms ,medicine ,Animals ,Mice, Inbred BALB C ,Innate immune system ,biology ,Macrophages ,MyD88 ,biology.organism_classification ,Interleukin-12 ,Immunity, Innate ,Disease Models, Animal ,Treatment Outcome ,Infectious Diseases ,medicine.anatomical_structure ,IL-12 ,Myeloid Differentiation Factor 88 ,Immunology ,Cancer research ,Interleukin 12 ,Original Article ,Parasitology ,Immunotherapy ,Bone marrow ,Toxoplasma - Abstract
Toxoplasma gondii is an intracellular protozoan parasite that causes a Th1 cellular immunity. Our previous study showed that T. gondii lysate antigen (TLA) treatment in S180 tumor-bearing mice resulted in tumor reduction by suppressing CD31 expression, a marker of angiogenesis. In the present study, to investigate tumor suppressive effect of TLA under the absence of T lymphocytes, athymic nude mice were compared with euthymic mice in the anti-tumorigenic effect triggered by TLA in CT26 tumors. According to the results, intratumorally injected TLA reduced tumor growth and TIMP-1 level, a metastatic marker, in both euthymic and athymic mice. TLA treatment led to a sharp increase in IL-12 expression in serum cytokine profiling of athymic mice, and increased MyD88 signals in macrophages derived from the bone marrow, implying the activation of innate immunity. The selective induction of IL-12 by TLA treatment had an anti-tumorigenic effect.
- Published
- 2014