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Plant growth regulator is an essential pesticide to date while the available active ingredient is not well understood unlike fungicide, insecticide and herbicide. This study was aimed to evaluate a new chemical class of plant growth regulator, and the total of 92 benzene derivatives were screened for their germination and early stage of the root growth regulation on Brassica campestris. Thirty benzaldehydes, nine acids, one amide, and one ester showed potent root growth inhibitory activity (>70 % inhibition) while only salicylaldehyde showed potent germination inhibition ($IC_{50}

The fruits of Acanthopanax sessiliflorus Seeman(Araliaceae) were extracted with 70% aqueous ethanol at roomtemperature. The concentrated extract was partitioned with ethylacetate (EtOAc), n-butyl alcohol, and H 2 O, successively. From theEtOAc fraction, two compounds were isolated through therepeated silica gel, octadecyl silica gel, and Sephadex LH-20column chromatographies. According to the results of physico-chemical and spectroscopic data including NMR, mass spectrometry,and infrared spectroscopy, the chemical structures of thecompounds were determined as 3,5-dihydroxycinnamic acid (1)and protocatechuic acid (2). Compound 1 was isolated from thefruits of A. sessiliflirus Seeman for the first time. And thecompounds were evaluated for the radical scavenging theantioxidant capacity using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)diammonium salt, 1,1-diphenyl-2-picrylhydrazy, andoxygen radical absorbance capacity assay.Keywords Acanthopanax sessiliflorus · 2,2'-azino-bis(3-ethyl-benzthiazoline-6-sulfonic acid)diammonium salt ·3,5-dihydroxycinnamic acid · 1,1-diphenyl-2-picrylhydrazyl · NMR · oxygen radical absorbance capacity · protocatechuic acid서론오가피(Acanthopanax sessiliflorus Seeman)는 두릅나무과(Araliaceae)에 속하는 식물로 낙엽활엽관목이다. 해발 700 m 이상의 고산지대에 자생하는 내건성 및 내한성 식물로, 한국, 일본 북해도,중국 만주 지역, 러시아의 우수리 지역에 분포하고 있다. 줄기에는 드물게 큰 가시가 있고, 잎은 호생하며 3~5 소엽으로 된장상복엽이다. 꽃은 산형화서로 무경 또는 단경이고 과실은 광타원형으로 9월에 검게 익으며 화주는 합생으로 갈라지지 않는다(Lee 등, 1996; Ryoo 등, 2003).오가피는 대한약전에 수재된 생약으로 “오갈피나무 A.sessiliflorus Seem. 또는*Corresponding author (N.-I. Baek: nibaek@khu.ac.kr) 동속식물(두릅나무과 Araliaceae)의 뿌

Repeated SiO 2 and octadecyl silica gel (ODS) columnchromatographies of the EtOAc fraction from Magnolia obovatafruits, 10 neolignans, named magnolol (1), honokiol (2),isoobovatol (3), isomagnolol (4), obovatol (5), obovatal (6), 9-methoxyobovatol (7), magnobovatol (8), obovaaldehyde (9), and2-hydroxyobovaaldehyde (10) were isolated and identified. Allisolated compounds were evaluated for in vitro cytotoxicityagainst seven human cancer cell lines.Keywords cancer cell · cytotoxicity · honokiol · Magnolia obovata · magnolol · neolignanMagnolia obovata Thunb. is a deciduous tree that is distributedthroughout Korea, China, and Japan. This plant has been used forthe treatment of fever, headache, diarrhea, anxiety, and relief ofasthma in Chinese medicine. M. obovata has been reported tohave anti-platelet (Pyo et al., 2002a; 2002b), anti-gastritic (Cho etal., 2008), anti-inflammatory (Tzeng and Liu, 2004; Seo et al.,2013) and cytotoxic (Min et al., 2008; Youn et al., 2008) activities.So far, magnolol, honokiol, and obovatol have been reported toshow cytotoxic activities against human cancer cells (Kim andRyu, 1999; Lin et al., 2001; Yang et al., 2003; Youn et al., 2008;Patrick et al., 2011). However cytotoxic effects of neolignans fromM. obovata fruits have never been reported. Therefore, in thepresent study, the neolignans from M. obovata fruits wereevaluated for cytotoxic effects against human cancer cell lines invitro using the micro culture tetrazolium (MTT) assay. We isolated10 neolignans from M. obovata fruits using SiO