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Combining ability and heterosis analysis was done to identify the potential parents and best performing hybrids for seed cotton yield, yield contributing traits and fiber quality parameters. Six lines, viz., VH-259, IUB-222, CRS-456, AA-703, KZ-191, PB-39 and four testers PB-39, CIM-608, BH-163 and PB-900 were crossed in line × tester crossing fashion to develop 20 F1 hybrids. Analysis of variance displayed significant differences among parents and hybrids indicating the presence of genetic variability except fiber fineness. d2GCA/d2SCA ratio depicted the predominance of non-additive types of gene action for plant height, number of monopodial branches, number of sympodial branches, numbers of bolls, boll weight, seed cotton yield, lint percentage, fiber length and fiber strength. The parents VH-259, CRS-456 and AA-703 among lines and PB-39 among testers displayed significant GCA effects for most of the traits. VH-259 × PB-39, CRS-456 × CIM-608 and AA-703 × PB-900 registered significant SCA effects and positive heterosis and hetrobeltiosis for most of the trait investigated. [ABSTRACT FROM AUTHOR]

The present investigations were carried out to study the genetic variability, coefficient of variations, heritability, genetic advance and correlation among 20 F1 hybrids and 9 parents in upland cotton. Line × tester mating design was deployed to develop crosses and 29 cotton genotypes were field planted in randomized complete block design (RCBD) in three replications to assess the genotypes for plant height (cm), number of sympodial branches, number of monopodial branches, seed cotton yield (g), number of bolls, boll weight (g), lint percentage (%), fiber strength (g/tex), fiber length (mm) and fiber fineness (µg/inch). Analysis of variance revealed significant differences at 1% level of significance for all traits except fiber fineness. The mean performance of F1 populations were better than parents. Maximum GCV was recorded for number of monopodial branches followed by number of sympodial branches and seed cotton yield. High broad sense heritability estimates were reported for seed cotton yield and all yield contributing traits except fiber quality with high response to selection. High heritability with genetic advance suggested vigorous phenotypic selection in breeding population for the improvement of crop traits. The correlation coefficient depicted that seed cotton yield is positively associated with other yield contributing traits suggesting that more than one trait can be used as indirect selection criteria in segregating populations. [ABSTRACT FROM AUTHOR]

Major intrinsic proteins (MIPs) of chickpea (Cicer arietinum L.), barrel medic (Medicago truncatula L.) and maize (Zea mays L.) were targeted in current studies. Amino acid sequences of major intrinsic proteins of chickpea, barrel medic and maize were retrieved from NCBI database followed by BLASTP. Pairwise and multiple alignment of sequences was done by using ClustalX software, and phylogenetic trees were constructed by using MEGA5 software. Medicago truncatula found to have total 35 MIPs which further comprised of 10 MtPIPs, 6 MtTIPs, 17 MtNIPs and 2 MtSIPs. Cicer arietinum genome comprised of 41 MIPs which were consisted of 10 CaPIPs, 12 CaTIPs, 15 CaNIPs and 4 CaSIPs protein sequences. Genome of Zea mays was carrying 36 ZmMIPs which were categorized into 12 ZmPIPs, 13 ZmTIPs, 8 ZmNIPs and 3 ZmSIPs. Phylogenetic trees were constructed separately for every sub-families of barrel medic, chickpea and maize. PIPs were consisted of two main groups for all of three crops which were named as PIP-A and PIP-B. NIPs clustered into six subgroups which were named as NIP-1, NIP-2, NIP-3, NIP-4, NIP-5, and NIP6. TIPs and SIPs were scattered in four groups and entitled as TIP-1, SIP-1, TIP-2, SIP-2, TIP-3, SIP-3 and TIP-4 respectively. Collective phylogenetic tree was constructed for all of MIPs which showed that PIPs, TIPs, NIPs and SIPs of all of three crops clustered in respective groups and no outliers were present. [ABSTRACT FROM AUTHOR]