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The use of sanitizers in the COVID19 pandemic is very common. While using sanitizers in Nepal, we have found people having problems with skin irritation and rashes; hence it was felt to know the amount of glycerol used as an emollient. A validated UV visible spectrophotometric and Reverse-phase High-performance liquid chromatography (RP-HPLC) method was designed to determine the amount of glycerol in the locally available sanitizers. The glycerol in sanitizers showed variation in amount, ranging from 0.78 to 1.66 g and 0.75 to 1.62 g/100 mL by HPLC and UV, respectively. The sanitizer samples with less purity or failed to meet the specification limit should be withdrawn, and their use must be limited. Hence this method seems to be easy, reliable, and cost-effective for determining glycerol based on the chemical derivatization technique. [ABSTRACT FROM AUTHOR]