1. Molecular detection of Coxiella burnetii in tick and blood samples from small ruminants in northwest of Iran.
- Author
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Ghazanabad AE, Esfandiari N, Najafi M, Mehrabi S, Sarani S, Khademi P, and Maurin M
- Subjects
- Animals, Iran epidemiology, Sheep, Tick Infestations veterinary, Tick Infestations epidemiology, Argasidae microbiology, Female, Polymerase Chain Reaction veterinary, Male, Coxiella burnetii isolation & purification, Coxiella burnetii genetics, Goats, Goat Diseases epidemiology, Goat Diseases microbiology, Goat Diseases parasitology, Sheep Diseases epidemiology, Sheep Diseases microbiology, Sheep Diseases parasitology, Ixodidae microbiology, Q Fever veterinary, Q Fever epidemiology
- Abstract
This survey sought to molecularly detect Coxiella burnetii in Argasidae and Ixodidae ticks attached to small ruminants in the region of West Azerbaijan (Northwest of Iran) and blood samples collected from the same animals. 451 tick samples and 927 blood samples were obtained from sheep (n = 536) and goats (n = 391) and tested by nested PCR for detection of C. burnetii insertion sequence IS1111 or icd gene sequence. The collected ticks were morphologically classified as Rhipicephalus sanguineus, Rhipicephalus turanicus, Hyalomma asiaticum, Hyalomma anatolicum, or Argas reflexus. 14% of ticks (65 in total 43 for IS1111 and 22 for icd gene) tested positive for C. burnetii, none of which were from the Argas genus. Among the 927 blood samples, 218 (23.5%) tested positive for C. burnetii. The positive result from analysis targeting the genes IS1111 and icd were 131 and 87 respectively. As Q fever is a tickborne zoonosis and endemic to Iran, such information is critical for creating effective, coordinated, and strategic tick and pathogen control programs to prevent disease outbreak in domestic animals and humans., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
- Published
- 2024
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