1. Cloning and characterization of a second form of the rice adenine phosphoribosyl transferase gene (OsAPT2) and its association with TGMS.
- Author
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Zhou CJ, Li J, Zou JC, Liang FS, Ye CJ, Jin DM, Weng ML, and Wang B
- Subjects
- Adenine chemistry, Adenine Phosphoribosyltransferase chemistry, Adenosine chemistry, Adenosine Monophosphate metabolism, Alleles, Amino Acid Sequence, Blotting, Northern, Cloning, Molecular, Computational Biology methods, DNA Primers chemistry, DNA, Complementary metabolism, Down-Regulation, Exons, Gene Expression Regulation, Plant, Genes, Plant, Genetic Vectors, Homozygote, Hot Temperature, Introns, Light, Models, Genetic, Molecular Sequence Data, Nucleotides chemistry, Oligonucleotides, Antisense chemistry, Oryza enzymology, Phenotype, Plant Leaves metabolism, Plant Physiological Phenomena, Pollen metabolism, Polymerase Chain Reaction, Protein Structure, Secondary, RNA, Messenger metabolism, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Amino Acid, Temperature, Transgenes, Adenine Phosphoribosyltransferase genetics, Oryza genetics
- Abstract
A rice gene, OsAPT2, which encodes a putative adenine phosphoribosyl transferase (APRT), was cloned and characterized. Analysis of the cDNA and genomic sequences revealed seven exons and six introns in the OsAPT2. The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously isolated APRTs. RT-PCR analysis indicated that the OsAPT2 transcript in the young panicles of 'Annong S-1' is down-regulated at 29 degrees C, the critical temperature for induction of 'Annong S-1' fertility conversion. Since the panicle is likely the thermo-sensitive organ at the early stages of pollen fertility alternation, the observed heat-induced change in the OsAPT2 expression pattern in young panicles may mediate, at least in part, thermo-sensitive genic male sterility (TGMS) in 'Annong S-1'. An antisense strategy was used to suppress the expression of the OsAPT2 homolog in Arabidopsis, and the obtained homozygous transgenic plants contained lower AMP content, displayed lower pollen germination rates and exhibited some abnormalities in leaf phenotypes and flowering timing. These data suggest that OsAPT2 is likely to be involved in TGMS in the rice line 'Annong S-1'.
- Published
- 2006
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