Search

Your search keyword '"de Boeck, S."' showing total 2 results
Start Over Author "de Boeck, S." Publisher kluwer academic/plenum publishers
2 results on '"de Boeck, S."'

2. Novel techniques for identification and characterization of proteins loaded on gels in femtomole amounts.

Author

Gevaert K, De Mol H, Verschelde JL, Van Damme J, De Boeck S, and Vandekerckhove J

Subjects
Amino Acid Sequence, Chromatography, High Pressure Liquid, Cloning, Molecular, DNA, Complementary genetics, Electrophoresis, Polyacrylamide Gel, Microchemistry methods, Molecular Sequence Data, Proteins chemistry, Proteins isolation & purification, Sensitivity and Specificity, Software, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Spectrophotometry, Ultraviolet, Acrylic Resins chemistry, Proteins analysis
Abstract

A combination of techniques is presented allowing gel-purified protein identification in the femtomole range using matrix-assisted-laser-desorption-ionization mass spectrometry. The proteins are detected in the primary gel by a sensitive negative staining procedure, transferred, and concentrated in a secondary gel matrix. There, they are digested in the presence of H218O and their sequences are predicted (1) by peptide mass fingerprinting, (2) by comparing the post-source-decay (PSD) spectra with theoretical spectra of candidate isobaric peptides using a computer algorithm called MassFrag, and (3) by a manual readout of the 18O/16O-labeled fragmentation ions in the PSD spectra.

Published
1997
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results